OBJECTIVE:To develop an HPLC method for the content determination of loratadine in human plasam.METHODS:The separation was performed on Kromasil C18(200 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-water-0.5 mol?L-1 potassium phosphate-phosphoric acid(52:43:5:0.1) at flow rate of 1.0 mL?min-1.The detection wavelength was set at 275 nm and injection volume was 20 ?L.The column temperature was set at room temperature with diazepam as internal standard.RESULTS:The good linearity was obtained within 0.5～100 ?g?L-1(r=0.999 3).The detection limit was 0.5 ?g?L-1 and the recovery ranged during 99.71%～102.41%.The absolute recovery was within 80.72%～83.78%.The RSD of intra-day and inter-day were less than 11%.CONCLUSION:Established method could be used for the pharmacokinetics and bioavailability study of loratadine.

Objective To analyse the effect of octreotide combined with compound coenzyme on Th17/CD4 +CD25 +Foxp3 + regulatory T cells imbalance in patients with purulent appendicitis.Methods 150 patients with acute suppurative appendicitis were randomly divided into experimental group and control group, 75 cases in each group.Patients of two groups underwent appendicitis laparoscopic resection, then the control group were given conventional treatment and experimental group were given subcutaneous injection of octreotide on the basis of conventional treatment.Th17, CD4 +CD25 +Foxp3 +regulation T cells ( Treg) , and Th17 /Treg balance were analyzed.ResuIts Compared with control group, Th17/Treg imbalance of experimental group recovered better: the proportion of Th17, Treg, and Th17/Treg ratio decreased (P<0.05); the expression levels of related transcription factor, RORγt and Foxp3 mRNA decreased (P<0.05); serum cytokine (IL-4, IL-6, IL-10, IL-17, IL-23 and TGF beta) and C-reactive protein (CRP) content decreased (P<0.05);serum interferon gamma (IFN-gamma) content increased (P<0.05).ConcIusion Octreotide combined with compound coenzyme could correct Th17/Treg imbalance in suppurative appendicitis patients, improve the immune function of patients and affect the prognosis of patients.