OBJECTIVE: To establish a detection method for zirconium and its compounds in the workplace air with inductively coupled plasma-mass spectrometry( ICP-MS). METHODS: The samples in workplace air were collected by micro filtration membrane. The water soluble zirconium compounds were eluted with 2. 0 mol / L hydrochloric acid and then pre-digested by nitric acid. The water insoluble zirconium compounds were digested by ammonium sulfate solution and then determined by ICP-MS. RESULTS: There were good linear relationship in the range of 0. 000-1. 000 mg / L, and the correlation coefficient was 0. 999 9. The detection limit was 0. 1 μg / L,and the minimum detection concentration was 0. 07μg / m~3(sample volume was 75 L). For the water soluble zirconium compounds the average recovery of standard addition was 95. 0 %-98. 8 %. The with-in run relative standard deviation( RSD) was 2. 0 %-3. 9 % and the between-run RSD was2. 8 %-6. 6 %. For the water insoluble zirconium compounds, the average recovery of standard addition was 94. 4 %-98. 7 %. The with-in run RSD was 2. 1 %-4. 2 % and the between-run RSD was 3. 2 %-4. 7 %. The samples could be stored at room temperature for at least 30 days. CONCLUSION: The method is rapid,accurate and high sensitivity. It can be used for quantitative detection of zirconium and its compoundsin workplace air.

Objective: To study the effects of single photon emission computed tomography/computed tomography (SPECT/CT) and magnetic resonance imaging (MRI) in the diagnosis of primary bone tumors. Methods: A total of 104 patients with primary bone tumors in Zibo Central Hospital hospital from January 2016 to January 2018 were enrolled in this study. SPECT/CT and MRI examinations were performed on these patients, and the results of the examinations were compared with the results of surgical pathology. The diagnostic efficacy of the two methods for primary bone tumors were compared. Results: ⑴Results of pathological examination: 70 cases were malignant tumors, and 34 cases were benign tumors; results of SPECT/CT: 72 cases were malignant tumors, and 32 cases were benign tumors; MRI: 76 cases were malignant tumors, and 28 cases were benign tumors; there was no difference between the two methods in the diagnosis of primary bone tumors. ⑵ The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of SPECT/CT were 92.86%, 79.41%, 88.46%, 90.28% and 84.38% respectively, slightly higher than those of MRI (91.43%, 64.71%, 82.69%, 84.21% and 78.57%), but the difference was not statistically significant (P>0.05). ⑶ There was a high consistency between SPECT/CT and MRI in the diagnosis of primary bone tumors (Kappa value=0.613). ⑷ SPECT/CT is more accurate in the diagnosis of osteosarcoma, giant cell tumor, myeloma and fibrous dysplasia, while MRI is more accurate in the diagnosis of osteosarcoma, chordoma, Ewing's sarcoma and schwannoma. Conclusions SPECT/CT fusion imaging and MRI have high sensitivity and accuracy in the diagnosis of primary bone tumors, and the results of the two diagnostic methods have high consistency. SPECT/CT has a high accuracy in the diagnosis of ossification and calcification, while MRI has a high accuracy in the diagnosis of bone tumors in bone soft tissue and nervous system. Therefore, the joint application of the two methods can make up for each other's shortcomings and make the diagnosis of primary bone tumor more accurate.

OBJECTIVE: To find out the relativity between the time when the traumatic prolapse of intervertebral disc occurred and the change of the CT numerical value. METHODS: The CT numerical value of the vertebral pulp of herniation (denoted as A) and the CT numerical value of the vertebral pulp without herniation (denoted as B) were measured in for 198 patients and analyzed the relation between this parameter and the time when the prolapse of intervertebral disc occurred. RESULTS: It's found find out that there is relativity between the parameter and the time when the prolapse of intervertebral disc occurred, and this difference has the value for the statistics, when the parameter is at about -12 to -40 HU, the average value is -25.8 HU (A < B), it can be determined the prolapse of intervertebral disc occurred within 3 months ago. When the parameter is at about -13 to 0 HU, the average value is -6.6HU (A is similar with B), it can be determined the prolapse of intervertebral disc occurred at about 4-6 months ago. When the parameter is at about 37 to 289HU, the average value is 96.1 HU (A > B). It can be determined the prolapse of intervertebral disc occurred more than 12 months ago. CONCLUSION The approximate time can be determined when the prolapse of interverterbral disc occurred by the paremeter.
Adolescent ; Adult ; Female ; Hernia/pathology* ; Humans ; Intervertebral Disc Displacement/etiology* ; Lumbar Vertebrae/pathology* ; Male ; Middle Aged ; Time Factors ; Tomography, Spiral Computed ; Young Adult

AIM: To compare the clinical efficacy of the Balanced energy system versus the conventional torsional ultrasound system in phacoemulsification surgeries for cataracts with varying nuclear hardness.METHODS: In this study, 120 patients(122 eyes)with age-related cataracts scheduled for surgery between November 2021 and November 2022 at our hospital were randomly divided into two groups: 58 patients(59 eyes)in the experimental group underwent surgery using the Balanced energy system, while 62 patients(63 eyes)in the control group were treated with the conventional torsional ultrasound system. Intraoperative cumulative dissipated energy(CDE), case time(CT), aspiration time(AST), and estimated fluid used(EFU)were recorded. Patients were followed-up for 3mo to examine and record the best-corrected visual acuity(BCVA)and corneal endothelial cell density(ECD), and to calculate the rate of endothelial cell loss.RESULTS: Comparing the intraoperative parameters between the two groups, there was no significant difference in CT(P>0.05), but the CDE, AST and EFU of the patients in the experimental group were lower than those of the control group(P<0.05), and the CDE of patients with grade III nuclear hardness in the experimental group was lower than the control group(P<0.05), CDE, AST and EFU in patients with grade IV nuclear hardness were lower than those in the control group(P<0.05). After 3mo of follow-up, BCVA in both groups improved significantly, and the experimental group recovered faster than the control group. At 3mo after surgery, the ECD of the two groups of patients was reduced compared with that before surgery(P<0.01), but there were no significant differences in ECD and endothelial cell loss rates between the experimental and control groups before and at 3mo after surgery(P>0.05). In grade IV nuclear hardness cataracts, the rate of endothelial cell loss in the experimental group was significantly lower than that in the control group(4.63%±4.10% vs. 6.63%±4.49%, P<0.01).CONCLUSION: The Balanced energy system and the conventional torsional ultrasound system both show high safety and efficiency in phacoemulsification of cataracts with different nuclear hardness. However, the former demonstrates substantial advantages in cases with dense nuclei, offering lower ultrasound energy, shorter aspiration and infusion times, and reduced volume of infusion fluid.

OBJECTIVETo study the value of hsCRP and Alb in evaluating the prognosis of patients with systemic lupus erythematosus (SLE).

METHODS126 SLE patients from January 2011 to January 2016 were enrolled in this study, and their clinical data were collected, including SLEDAI, hsCRP and Alb and complications. The correlation between hsCRP/Alb ratio and SLEDAI after treatment was analyzed. All patients were followed up after discharge, and the prognosis-related factors were analyzed.

RESULTSAfter treatment, hsCRP/Alb ratio of patients with SLEDAI 10-14 score was significantly higher than that of 5-9 and 0-4 score(P<0.05). hsCRP/Alb ratio was positively correlated with infection (r=0.574), renal damage (r=0.499) and cardiac injury (r=0.516) (P<0.05), while it demonstrated no correlation with blood system damage, CNS damage and lung injury(P>0.05). after treatment SLEDAI ≥10 score, hsCRP/Alb≥0.05 mg/g and complications significantly correlated with prognosis of patients(P<0.05).

CONCLUSIONhsCRP/Alb correlates with the prognosis of patients with SLE at a certam level.

Humans ; Lung ; Pneumoconiosis/diagnostic imaging* ; Retrospective Studies ; Silicosis/diagnostic imaging* ; Tomography, X-Ray Computed

OBJECTIVETo establish the method of detecting the concentrations of chlorpyrifos in air of workplace with high performance liquid chromatographic (HPLC).

METHODSAccording to standards of methods for determining the chemical substances in workplace air, chlorpyrifos in the air was collected by silicone tube, then dissolved by acetonitrile and determined by high performance liquid chromatography with UV-detector.

RESULTSThere was a linear relationship within the range of 0 ∼ 10.0 µg/ml, and regression equation was y = 5206.1x - 104.7, correlation coefficient was 0.9999, the detection limit was 0.006 µg/ml. The lowest detected concentration was 0.001 mg/m(3) (sampling volume 4.5 L). The average recoveries was 98.3% ∼ 102.5%. The within-run precision was 1.96% ∼ 4.39%, the between-run precision was 2.76% ∼ 5.87%. The desorption efficiencies were 99.0% ∼ 103.3% and the sampling efficiencies were 94%. The samples in silicone tube could be stored for 15 days at room temperature.

CONCLUSIONThe present method could meet with the requirements of Guide for establishing occupational health standards-Part 4 Determination methods of air chemicals in workplace and be feasible for determination of chlorpyrifos in workplace air.

Air Pollutants, Occupational ; analysis ; Chlorpyrifos ; analysis ; toxicity ; Chromatography, High Pressure Liquid ; methods ; Limit of Detection ; Reproducibility of Results ; Workplace

OBJECTIVEChildhood behavioral problems can predict future psychiatric disorders. Temperament development is important for a healthy personality in adulthood. This study investigated whether breastfeeding is associated with the occurrence of behavioral problems and the temperament development in preschool children.

METHODSA total of 737 children (399 boys and 338 girls) aged 4-5 years in Zibo City were recruited by stratified random cluster sampling. They were born at term with a birth weight of >/= 2.5 kg. The feeding patterns and the breastfeeding duration in infancy were collected. Behavioral and temperament developments were investigated by the Achenbach Child Behavior Checklist (CBCL), temperament questionnaire for 3-7-year-old children and a self-designed inventory questionnaire. The association of feeding patterns and the breastfeeding duration with behavioral problem occurrence and the temperament development in children were analyzed by a multivariate non-conditional logistic regression analysis and a multivariate stepwise regression analysis.

RESULTSAfter controlling for confounding variables, such as family income and parental education levels, it was found that a breastfeeding duration of >/= 9 months was a protective factor against behavioral problem occurrence in boys (OR=0.184). In girls, a breastfeeding duration of >/= 9 months was also a protective factor against behavioral problem occurrence (OR=0.165), while a mixed feeding with more breast milk and less formula milk was a risk factor (OR=2.203). The factors influencing temperament development consisted of exclusive formula feeding and the duration of breastfeeding (lasting for 4-6 months or 7-9 months) as well as a mixed feeding (with more formula milk and less breast milk, more breast milk and less formula milk, or equal amount of both).

CONCLUSIONSThe fewer amounts and the shorter duration of breastfeeding are risk factors for behavioral problems occurrence in children aged 4-5 years. Children's temperament development is correlated with the feeding patterns and the breastfeeding duration.

Breast Feeding ; Child Behavior Disorders ; etiology ; Child, Preschool ; Educational Status ; Female ; Humans ; Infant ; Infant, Newborn ; Logistic Models ; Male ; Mother-Child Relations ; Temperament

Objective: To analyze the base incidence, distribution characteristics, survival status and social security of occupational pneumoconiosis (hereinafter referred to as "pneumoconiosis") in Zibo City. Methods: The new pneumoconiosis patients in Zibo City from 1949 to 2021 were selected as the research subjects using a cross-sectional survey. Household survey or telephone follow-up were carried out, and the distribution characteristics, living conditions and social security situation were retrospectively analyzed. Results: A total of 8 910 patients with pneumoconiosis were investigated, and 96.0% of them were male. The stage Ⅰ pneumoconiosis patients accounted for 91.3%. From 1949 to 2021, the number of pneumoconiosis patients showed a stepwise upward trend with time. Most of the patients suffered from coal worker pneumoconiosis and silicosis, which accounted for 48.7% and 38.9%, respectively. The average age of onset was (52.7±11.4) years, and the average length of service exposed to dust was (21.1±9.4) years. The patients were concentrated in Zichuan District, Boshan District and Zhangdian District, that accounted for 87.8%. The industry distribution was mostly mining industry and manufacturing industry, accounting for 61.1% and 31.6% respectively. Among the 8 910 cases of pneumoconiosis, 543 cases were lost in follow-up. A total of 8 367 patients were followed-up, with a follow-up rate of 93.9%. The mortality rate of patients who completed follow-up was 50.5%, and the mortality rate decreased with the increase of the stage of pneumoconiosis (P<0.01). The rate of adoring social security in the 4 138 surviving patients was 98.4%. Conclusion: The situation of prevention and treatment of pneumoconiosis in Zibo City is challenging. It is necessary to strengthen the special control of dust hazards in mining and manufacturing industries in key areas such as Zichuan District, Boshan District and Zhangdian District.

OBJECTIVE To investigate the impact of evodiamine on the proliferation， migration and invasion abilities of trophoblastic cells induced by high glucose and its potential mechanism based on advanced glycation end products （AGE）/receptor for advanced glycation end products （RAGE） signaling pathway. METHODS Human trophoblastic cells HTR-8/SVneo were divided into control group， high glucose group， evodiamine low-dose group （2 μmol/L）， evodiamine high-dose group （4 μmol/L）， pc-NC group （transfected with pc-NC plasmid+4 μmol/L evodiamine）， and pc-RAGE group （transfected with pc-RAGE plasmid+ 4 μmol/L evodiamine）. Cells in all groups except for the control group were cultured in a high sugar （25 mmol/L glucose） environment， and cells in all groups except for the control group and the model group were transfected with the corresponding plasmids and/or received the corresponding drug interventions. The survival rate， apoptotic rate， scratch healing rate， and invasion number， as well as the protein and mRNA expressions of AGE， RAGE， nuclear factor- κB p65 （NF- κB p65）， matrix metalloproteinase-2 （MMP-2）， and MMP-9 were examined in each group. RESULTS Compared with the control group， the cell survival rate， scratch healing rate， invasions number， and the mRNA and protein expressions of MMP-2 and MMP-9 in the high glucose group significantly decreased （P＜0.05）， while the apoptotic rate， the mRNA and protein expressions of AGE and RAGE， the mRNA expression of NF-κB p65， and the phosphorylation level of NF-κB p65 protein significantly increased （P＜0.05）； compared with the high glucose group， the above indexes of cells in evodiamine low-dose and high-dose groups were significantly improved， and the effect of the high-dose group was significantly better than that of the low-dose group （P＜0.05）； overexpression of RAGE attenuated the ameliorative effect of evodiamine onthe above indexes in high glucose-induced trophoblast cells （except for AGE mRNA and protein） （P＜0.05）. CONCLUSIONS Evodiamine can promote the proliferation， migration and invasion of high glucose-induced trophoblast cells and ameliorate their functional impairment， and the above effects are associated with the inhibition of the AGE/RAGE signaling pathway.