Objective To study the immune response of dendritic cells(DC)modified with tumor antigen and SEB to transplanted tumor in nude mice.Methods Nude mice models were established with human colon cancer cells inoculated subcutaneously,grouped into control group,therapy group and retherapy group;DC was isolated and purified from PBMC of colon carcinoma patient to activate T lymphocyte with DC modified by tumour antigen and SEB.The T lymphocyte was implanted to the models to inhibit the growth of implanted tumor through tail vein.Tumor formation,growth and size,nude mice feeding and activity status were observed.Results The subcutaneously xenotransplanted tumor model of human colon carcinoma in nude mice was successfully established.After colon cancer cells had been inoculated in nude mice for 60 days,volume of transplanted tumor in the control group was(3 899?22)mm3,therapy group(1 301?16)mm3,and retherapy group(392?18)mm3.Compared with the control group,the tumor in therapy group and retherapy group could grow slowly(P=0.000).Compared with that in the therapy group,the tumor growth in retherapy group became obviously slower(P=0.000).Conclusion The immune response induced by DC can inhibit the growth of transplanted tumor in nudes mice,which may provide a new therapy for tumor.

Objective To establish the rabbit brain death model,and observe the change of kidney function and morphology in the brain death rabbits,fully evaluate the possibility of transplantation surgery carried out with brain death donor kidney.Method 40 male healthy New Zealand rabbits were randomly divided into sham group (n =20) and brain death group (n =20),and each group further was divided into four groups according to 2,4,6 and 8 h after brain death groups.At the end of the experiment,the blood samples and kidney tissues were collected.The level of blood urea nitrogen(BUN),serum creatinine (Cr) and the apoptosis of kidney cells were determined by automatic biochemistry analyzer and TUNEL method,respectively.The morphology changes of liver were detected by HE staining.Result Although BUN values were with no obviously alteration (P＞0.05) within 8 h after brain death,the renal Cr levels in the states of 2,4 and 8 h (P＜0.05) brain death were found significantly increased compared with shamed groups.The denaturalization in the epithelial cells of renal tubule,glomerulus atrophygradually,swelling and vacuolation of epithelial cells,edema in the interstitium and inflammatory cell infiltration and partly occlusion in proximal convoluted tubule gradually emerged in the brain dead groups.The inflammatory factor intercellular adhesion molecule (ICMA) and the apoptosis index in brain dead groups increased in a time dependent manner but the expressions of protective factor HSP70 conducted a contrary tendency.Conclusin Brain death could lead to the damage of kidney function and morphology and this injury aggravated in a time dependent manner.The alterations might be relevant to the production of inflammatory factors.Within 8 h after brain death there were obvious changes in the kidney function and morphology,which provide experimental basis for the rational use of brain death donor kidney transplantation.

Allografts ; COVID-19 ; China/epidemiology* ; Disease Outbreaks ; Humans ; Kidney Transplantation/adverse effects* ; SARS-CoV-2