2.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
3.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
4.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
5.Expression of vascular endothelial growth factors (VEGF)-A, -C and -D and their prognostic significance and relationship with angio- and lymphangiogenesis in gastric cancer.
Xiao-lei WANG ; Zi-sheng AI ; Jian-ping FANG ; Ru-yong TANG ; Xi-mei CHEN
Chinese Journal of Oncology 2008;30(11):837-843
OBJECTIVETo investigate the expressions of vascular endothelial growth factors (VEGF)-A, -C and -D and their prognostic significance and relation to angio- and lymphangiogenesis in gastric cancer.
METHODSThe expression of VEGF-A, -C and -D in 123 primary gastric cancers was detected by immunohistochemical staining. The lymphatic vessel density (LVD) and microvessel density (MVD) were assessed after immunohistochemical double-staining with D2-40 and CD34, respectively. The correlation between the expression of those VEGF factors and clinicopathological parameters were analyzed by univariate method. The overall survival was evaluated by Kaplan-Meier method and log-rank test. Multivariate analysis was carried out using Cox proportion hazard model.
RESULTSThe positive expression rate of VEGF-A, -C and -D in primary gastric cancer samples were 64.2%, 65.9% and 41.5%, respectively. High expression of VEGF-A, or -C or -D, or any two of them was correlated with high LVD (P < 0.05). High expression of both VEGF-A and -C was associated with high MVD, lymph node metastasis, LVI and MVI (P < 0.05). Both VEGF-C and -D high expression was correlated with LVI and lymph node metastasis (P < 0.05). The patients with high expression of these factors had a statistically shorter overall survival (P < 0.05). The patients with both VEGF-A and -C expression had the shortest survival (56 months). Multivariate analysis showed that VEGF-A high expression, MVD, lymph node metastasis and depth of tumor invasion were independent survival predictors (P = 0.033, 0.002, 0.019 and P < 0.001, respectively).
CONCLUSIONHigh expression of both VEGF-A and -C imply high potential of lymphangiogenesis, metastasis and poorer survival in gastric cancer patients. High expression of VEGF-C and -D may induce lymphangiogenesis and promote lymph node metastasis, but only VEGF-A is an independent predictor of survival.
Adult ; Aged ; Aged, 80 and over ; Female ; Follow-Up Studies ; Humans ; Lymphangiogenesis ; Lymphatic Metastasis ; Lymphatic Vessels ; pathology ; Male ; Microvessels ; pathology ; Middle Aged ; Neovascularization, Pathologic ; Proportional Hazards Models ; Stomach Neoplasms ; metabolism ; pathology ; Survival Rate ; Vascular Endothelial Growth Factor A ; metabolism ; Vascular Endothelial Growth Factor C ; metabolism ; Vascular Endothelial Growth Factor D ; metabolism
6.Study on tissue culture of pasqueflower.
Zi-xue ZHANG ; Wei-qun DING ; Yong TANG ; Wei-jing SHI ; Wen-cai YE
China Journal of Chinese Materia Medica 2004;29(3):215-218
OBJECTIVETo speed up seedling production of pasqueflower (Puzlsatilla chinenses) and their modernization in pasqueflower.
METHODWith tissue culture method, primary culture of different explants, culture of cluster buds and their rooting culture were conducted on medium of treatment combinations of adding different hormones.
RESULTThe appropriate medium for different culture stages were MS + 6-BA 1.0-3.0 mg x L(-1) + NAA 0-0.05 mg x L(-1) + Sucrose 30 g x L(-1) in primary culture, MS + 6-BA 0.2 mg x L(-1) + NAA 0.02 mg x L(-1) + BR 0.00001 mg x L(-1) + Sucrose 30 g x L(-1) in differentiation and subculture of cluster buds, 1/2 MS + NAA 0.4 mg x L(-1) + Sucrose 20 g x L(-1) in rooting.
CONCLUSIONApplying stem tip and flower buds as explants, high frequency propagation of seedlings can be achieved with plant tissue culture in Pasqueflower.
Flowers ; growth & development ; Plant Growth Regulators ; pharmacology ; Plant Roots ; growth & development ; Plant Stems ; growth & development ; Plants, Medicinal ; growth & development ; Pulsatilla ; growth & development ; Seedlings ; growth & development ; Tissue Culture Techniques ; methods
7.Role of oxotremorine in arginine vasopressin-induced hypothermia and its effects on behavioral thermoregulatory response in rats.
Zi-Ling SHEN ; Yong-Lu YANG ; Bing SUN ; Yu TANG ; Nian WANG
Chinese Journal of Applied Physiology 2012;28(2):107-112
OBJECTIVETo investigate the role of oxotremorine in arginine vasopressin (AVP)-induced hypothermia and its effects on the behavioral thermoregulatory response.
METHODSCore temperature (Tc), brown adipose tissue (BAT) temperature and motor activities were monitored in undisturbed female SD rats using radiotelemetry. The behavioral thermoregulatory response was monitored in rats using radiotelemetric temperature gradient apparatus. Effect of AVP (10 microg/kg) and oxotremorine (0.25 mg/kg) on Tc, motor activities, BAT temperature (T(BAT)), grooming activities and the behavioral thermoregulatory response were observed in rats.
RESULTSAdministration of AVP and oxotremorine caused a significant drop in Tc, T(BAT), and an increases in grooming activities, respectively. The hypothermic responses were accompanied with a preference for cooler ambient temperature. Oxotremorine augmented the reduction of Tc, T(BAT), and the elevation of grooming activities resulting from AVP, and lasting a longer time. Administration of oxotremorine followed immediately by AVP injection in rats was also shown to induce a preference for cooler ambient temperature, but there was no significant difference compared with AVP.
CONCLUSIONAVP-induced hypothermia was related with the set point temperature reduction, inhibiton of BAT thermogenesis and an increases in grooming activities. Oxotremorine could participate in peripheral AVP-induced hypothermia by affecting BAT thermogenesis and behavioral thermoregulation.
Adipose Tissue, Brown ; drug effects ; physiology ; Animals ; Arginine Vasopressin ; pharmacology ; Behavior, Animal ; Body Temperature Regulation ; Female ; Hypothermia, Induced ; Oxotremorine ; pharmacology ; Rats ; Rats, Sprague-Dawley
8.Simultaneous telemetric analyzing of the temporal relationship for the changes of the circadian rhythms of brown adipose tissue thermogenesis and core temperature in the rat.
Yong-lu YANG ; Zi-ling SHEN ; Yu TANG ; Nian WANG ; Bing SUN
Chinese Journal of Applied Physiology 2011;27(3):348-352
OBJECTIVETo measure simultaneously the time course for the circadian rhythm of brown adipose tissue(BAT) thermogenesis and core temperature, and analyzing their temporal relationship.
METHODSThe circadian rhythm of core temperature (Tc), BAT temperature (T(BAT)), axillary temperature (Tax) and motor activity were simultaneously measured by telemetry in adult male Sprague-Dawley rats at an ambient temperature of 22 degrees C during a 12-h light:12-h dark photoperiod (lights on at 06:00 h and lights off at 18:00 h).
RESULTS(1) T(BAT) was 0.67 degrees C lower than Tc group under the light phase, but it was similar to that Tc during the dark phase. The rate of increase in T(BAT) was higher than corresponding increases in Tc at the start of transition from the light to dark phase, and increase in T(BAT) commenced approximately 8 min before Tc increases. Whereas at the start of transition from the dark to light phase, decrease in T(BAT) commenced approximately 4 min before Tc decreases. (2) The amplitude of the circadian Tax rhythm was similar to that of Tc. During either the light phase or dark phase, Tax was lower than simultaneous measurement of Tc. (3) Increases in behavioral activity commenced before increases in T(BAT) and Tc at the start of transition from the light to dark phase.
CONCLUSIONBAT thermogenesis contributes to increase in core temperature during the dark phase, indicating that circadian changes of BAT thermogenesis does indeed play significant role in the overall maintenance of the circadian rhythm of core temperature.
Adipose Tissue, Brown ; metabolism ; physiology ; Animals ; Body Temperature ; physiology ; Circadian Rhythm ; physiology ; Male ; Rats ; Rats, Sprague-Dawley ; Telemetry ; methods ; Thermogenesis ; physiology
9.Protein expressions of S100B and calcitonin gene related peptide and pathological changes of the hippocampus in rats with kainic acid-induced epilepsy
Jun-Lin FENG ; Jing-Zi JIANG ; Hao LI ; Xiao-Hui LIN ; Yong-Gang TANG ; Jian-Hua LU
Chinese Journal of Neuromedicine 2011;10(3):255-259
Objective To investigate the expressions of S100B and calcitonin gene related peptide (CGRP) and the pathologic alterations of the hippocampus in kainic acid (KA)-induced epileptic rats. Methods Male SD rats were randomly divided into control group (n=8) and model group (n=40).Animal models of temporal lobe epilepsy were established by intracerebroventricular injection of KA; the same volume of saline was injected into the rats in the control group. Hippocampal tissues within various phases after seizures (6, 12, 24 and 72 h, and 24 h after the success of model making) were performed Nissl staining, Timm staining and immunohistochemical staining. The expressions of S100B and CGRP were observed, and the pathologic alterations of the hippocampal neurons and glial cells were studied.Results All rat models were successfully induced with epileptic seizures. Nissl staining showed that pyknotic neuronal necrosis appeared in the CA3 area of the hippocampus in the model group with cell body atrophy and disappearance of Nissl bodies 1 week after the injection. Timm staining showed that brown particles showed stripped distribution in the CA3 area of the hippocampus and some brown particles in the molecular layer of fascia dentate. Immunohistochemical staining indicated that significant neurons lost and gliosis appeared after seizures with abundant expressions of S100B and CGRP.Conclusion KA-induced epileptic rats express abundant S100B and CGRP and appear such pathological changes as disappearance of Nissl bodies and mossy fiber sprouting, indicating that both S100B and CGRP participate in the onset of epilepsy.
10.A new mutation in the GJB1 gene of a Chinese family with Charcot-Marie-Tooth disease associated with vocal cord paresis.
Qing-hua LI ; Kai-xiang LIU ; Jun-lin FENG ; Ai-yuan ZENG ; Hao LI ; Lan WU ; Yong-gang TANG ; Mei-lin CHEN ; Xiao-hui LIN ; Jing-zi JIANG
Chinese Journal of Medical Genetics 2010;27(5):497-500
OBJECTIVETo report an X-linked dominant Charcot-Marie-Tooth disease (CMTX) Chinese family with vocal cord paresis and to identify the mutation of gap junction protein beta 1 gene (GJB1).
METHODSPart of the family members with dysphagia, dysphonia and lethal respiratory failure were studied through flexible laryngoscope, clinical, brain MRI and electrophysiological examinations. After excluding large fragment tandem duplication containing peripheral myelin protein 22 gene (PMP22), direct sequencing was performed to analyze the mutation of the GJB1 gene in 5 patients including the proband, 5 unaffected family members and 50 unrelated healthy individuals.
RESULTSEight members spanning 3 generations in this family were affected with CMTX characterized by progressive atrophy and weakness of the anterior tibial and peroneal muscles, especially in the proband. Vocal cord paresis was observed through flexible laryngoscope in total of 4 affected members with dysarthria and dysphagia, 2 of them died of severe respiratory failure due to complete bilateral vocal cord involvement. Normal brain MRI was observed in the proband. The electrophysiological data showed predominant demyelization involving the motor and sensory nerves in the proband. DNA sequencing revealed a de novo c.186 C>G missense mutation in exon 2 of the GJB1 gene, the mutation cosegregated with phenotype.
CONCLUSIONRespiratory failure associated with vocal cord involvement may be a rare and severe symptom in CMTX. The present report provides further evidence for clinical and genetic heterogeneity in the X-linked Charcot-Marie-Tooth disease.
Adolescent ; Adult ; Asian Continental Ancestry Group ; genetics ; Base Sequence ; Case-Control Studies ; Charcot-Marie-Tooth Disease ; genetics ; Connexins ; genetics ; Female ; Humans ; Male ; Molecular Sequence Data ; Mutation, Missense ; Myelin Proteins ; genetics ; Pedigree ; Vocal Cord Paralysis ; genetics ; Young Adult