1.Stability analysis of allelopathic effects of Panax notoginseng on main crops by AMMI model.
Zi-long ZHANG ; Jun-ling HOU ; Wen-quan WANG
China Journal of Chinese Materia Medica 2015;40(2):191-197
This paper is aimed to study the differences of allelopathic effects of Panax notoginseng under different allelopathic chemicals resources and selection of appropriate rotation crops. The additive main effects and multiplicative interaction ( AMMI) model had been used to evaluate the stability of allelopathic effects of P. notoginseng on the varieties of corn, wheat and rice properly. The model could use not only to evaluate the stability of non-regional trial data but also explore the interaction between the rotation crop genotypes and donor substances more efficiently. Meanwhile, correspondence analysis can be used in the AMMI to evaluate genotype stability and donor substances. Ejingza No. 1 (g6) had stronger allelopathic effects with high stability, but Yunrui No. 1 (g9) which was appropriate rotation crop genotype, had weaker allelopathic effects with high stability. These findings will aid in choosing appropriate rotation crops and establishing proper rotation system.
Allelopathy
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Crops, Agricultural
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Panax notoginseng
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chemistry
2.Allelopathic effects of aqueous extracts from Panax notoginseng on three maize varieties (Zea mays).
Zi-Long ZHANG ; Jun-Ling HOU ; Wen-Quan WANG ; Zhi-Xin ZHANG ; Shi-Xiu ZHANG
China Journal of Chinese Materia Medica 2014;39(4):594-600
It has been showed that there were obvious obstacle effects of Panax notoginseng replanting. Crop rotation was the main effective technique to overcome the obstacle. To find a reasonable crop rotation system for P. notoginseng, aqueous extracts from root, stem and leaf of P. notoginseng were analyzed for allelopathic effect on three maize varieties (which are often grown in regions where P. notoginseng grown). The main results were as follows: (1) Allelopathic effect of P. notoginseng stem and leaf extracts on the three other tested plants was stronger than that of root extracts; (2) Corn was more vulnerable to the effects of allelochemicals at seedling stage than at germination stage, and the corn root was more sensitive than aerial part to allelochemicals; (3) Lusan No. 3 and Yunrui No. 1 showed resistance to P. notoginseng allelopathy, with respective comprehensive sensitivity indexes (M3) of - 0.089 3 and -0.159 2, while Bainuo No. 1 is sensitive at M3 = -0.261 0. It then can be concluded that Lusan No. 3 and Yunrui No. 1 may be an alternative rotation plants for overcoming P. notoginseng continuous cropping obstacle.
Allelopathy
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Panax notoginseng
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chemistry
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growth & development
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Pheromones
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pharmacology
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Plant Extracts
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pharmacology
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Plant Leaves
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chemistry
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Plant Roots
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chemistry
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Plant Stems
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chemistry
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Zea mays
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drug effects
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growth & development
3. Advances in biotechnological production of santalenes and santalols
Wen-long ZHA ; Jia-chen ZI ; Wen-long ZHA ; Jia-chen ZI
Chinese Herbal Medicines 2021;13(1):90-97
Sandalwood essential oil has been widely used not only as natural medicines but also in perfumery and food industries, with sesquiterpenoids as its major components including (Z)- α-santalol and (Z)-β-santalol and so on. The mature heartwoods of Santalum album, Santalum austrocaledonicum and Santalum spicatum are the major plant resources for extracting sandalwood essential oil, which have been overexploited. Synthetic biology approaches have been successfully applied to produce natural products on large scale. In this review, we summarize biosynthetic enzymes of santalenes and santalols, including various santalene synthases (STSs) and cytochrome P450 monooxygenases (CYPs), and then highlight the advances of biotechnological production of santalenes and santalols in heterologous hosts, especially metabolic engineering strategies for constructing santalene- and santalol-producing Saccharomyces cerevisiae.
4.Expression of EIIIA+ fibronectin in incised wound of rat's skin.
Meng HE ; Hai ZHAO ; Yi-wen SHEN ; Ai-min XUE ; Long CHEN ; Yun-ju GU ; Zi-qin ZHAO
Journal of Forensic Medicine 2014;30(6):409-412
OBJECTIVE:
To explore the relationship between the expression of EIIIA+ fibronectin in incised wound of rat's skin and injury time.
METHODS:
The wounding model was established by cutting the dorsal skin of 48 adult SD rats. The rats were sacrificed at the pre-set injury time as immediately, 0.5 h, 1 h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin samples were taken at the margin of wound. The expression of the EIIIA? fibronectin was detected by immunohistochemistry and Western blotting and the relationship be- tween its expression and injury time was observed. Results The expression of EIIIA+ fibronectin was not observed immediately. The basal cell of skin began to show positive expression 0.5 h after injury. With the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the Western blotting analysis.
CONCLUSION
The expression of EIIIA+ fibronectin could be used for estimation of injury time in the early stage of skin injury.
Animals
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Fibronectins/metabolism*
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Immunohistochemistry
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Proteins/metabolism*
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Rats
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Rats, Sprague-Dawley
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Skin/metabolism*
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Staining and Labeling
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Time Factors
5.Spectrum-effect relationship of reducing phlegm effect of Peucedanum harrysmithii var. subglabrum.
Jian-di LIANG ; Liang-gong ZHAO ; Xiao-hua LIU ; Wen LI ; Zi-long DANG ; Jin LIANG ; Shi-lan FENG
China Journal of Chinese Materia Medica 2012;37(19):2894-2897
OBJECTIVETo analyze the relationship between high-performance liquid chromatography (HPLC) fingerprints of the chloroform extract fractions of Peucedanum harrysmithii var. subglabrum (PHS) and its phlegm-reducing effect, in order to establish "active component group for reducing phlegm".
METHODHPLC was adopted to determine and analyze HPLC fingerprints of chloroform extract fractions of PHS. Phenol red expectorant experiment was used to observe the phlegm-reducing effect in mice. Mice were administered intragastrically with chloroform extract fractions for 6 days (1.4 g x kg(-1)), with acute bronchitis syrup as the positive control drug (12 mL x kg(-1)). The phenol red secretion in mice was determined by spectrophotometer. Then the grey relational analysis was used to study the spectrum-effect relationship.
RESULTThe phlegm-reducing effect of the chloroform extract fractions of PHS were resulted from the combined effect of all of its chemical components. Its various characteristic peaks represented different chemical components, and the order of their contributions to the phlegm-reducing effect was (number of peaks) 13 > 12 > 16 > 18 > 19 > 6 > 20 > 14 > 1 > 11 > 15 > 10 > 17 > 2 > 5 > 4 > 7 > 3 > 8 > 9, in No. 1, 3, 4, 10, 13 and 16 characteristic peaks were identified as marmesin, psoralen, xanthotoxin, Pd-Ib, pteryxin and peuformosin.
CONCLUSIONThe chloroform extract fractions of PHS show strongly phlegm-reducing effect. There may be certain relationship between their HPLC fingerprint and phlegm-reducing effect.
Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Ferns ; chemistry ; Mucus ; drug effects
6.Effects of chronic cadmium loading on the testis and endocrine function of reproduction in male rats.
Long CHEN ; Wen-Hua REN ; Shan-Liang ZHU ; Wei GAO ; Juan ZHOU ; Ying-Zi JIANG ; Yu GU
Acta Physiologica Sinica 2002;54(3):258-262
Sixty healthy Sprague-Dawley male rats were used and divided randomly into control group (group C), cadmium loading group with medium dose (group M) and cadmium loading group with high dose (group H). Groups C, M and H were orally dosed daily with 0, 5 and 10 mg/kg of cadmium for over 6 weeks. Effects of cadmium loading on testis and endocrine function of reproduction in male rats were studied. The results showed that the zinc content decreased slightly in testis and plasma, and the cadmium concentration increased significantly in the testis of groups M and H; while the plasma levels of cadmium and zinc had no obvious difference as compared with those of group C; daily sperm production in the testis of group H decreased markedly during week 3 of cadmium loading, and was significantly lower in groups M and H as compared to that in group C during week 6; alkaline phosphatase (ALP) in group H and lactate dehydrogenase-X (LDH-X) in groups M and H were markedly lower compared to those of group C; plasma testosterone (T) level in both cadmium loading groups decreased and was low or significantly lower than that in group C; follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels had no apparent difference between the three groups. It is suggested that the gradual accumulation of cadmium in testis tissue induced by chronic cadmium loading results in changes in some enzyme activity, a decrease in sperm production, and defect of endocrine function activity in the testis.
Alkaline Phosphatase
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drug effects
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Animals
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Cadmium
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blood
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Cadmium Chloride
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administration & dosage
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toxicity
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Follicle Stimulating Hormone
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blood
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Isoenzymes
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drug effects
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L-Lactate Dehydrogenase
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drug effects
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Luteinizing Hormone
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blood
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Male
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Reproduction
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drug effects
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Spermatogenesis
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drug effects
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Testis
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enzymology
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pathology
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Testosterone
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blood
7.Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B.
Ze-long JIAO ; Lei LI ; Zi-gang ZHAO ; Dan LIU ; Bi-wen LIN ; Heng-jin LI
Chinese journal of integrative medicine 2013;19(10):741-748
OBJECTIVETo investigate the antiinflammatory activities of aqueous extract of Occimum gratissmium (OGE) with emphasis on expression of proinflammatory cytokines in Lipopolysaccharide (LPS)-stimulated epithelial cell BEAS-2B.
METHODSEffects of OGE on cell viability were determined by MTT assay. mRNA expression were analyzed by and reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Activation of kinase cascades was investigated by immunoblot. Intracellular reactive oxygen species (ROS) was analyzed by flow cytometry.
RESULTSOGE (<200 μg/mL) treatment or pretreatment and following LPS exposure slightly affected viability of BEAS-2B cells. Increase of interleukin (IL)-6 and IL-8 and the elevated level of intracellular ROS in LPS-stimulated BEAS-2B cells were diminished by OGE pretreatment in a dose-dependent manner. OGE suppressed inflammatory response-associated mitogen-activated protein kinases (MAPKs) and Akt activation. Additionally, OGE pretreatment increased level of cellular inhibitor of κBα (IκBα) and inhibited nuclear translocation of nuclear factor kappa B (NF-κB).
CONCLUSIONThese findings indicate that significant suppression of IL-6 and IL-8 expressions in LPS-stimulated BEAS-2B cells by OGE may be attributed to inhibiting activation of MAPKs and Akt and consequently suppressing nuclear translocation of NF-κB.
Cell Nucleus ; drug effects ; metabolism ; Cell Survival ; drug effects ; Cytosol ; drug effects ; metabolism ; Epithelial Cells ; drug effects ; enzymology ; metabolism ; Gene Expression Regulation ; drug effects ; Humans ; I-kappa B Proteins ; metabolism ; Interleukin-6 ; genetics ; metabolism ; Interleukin-8 ; genetics ; metabolism ; Intracellular Space ; drug effects ; metabolism ; Lipopolysaccharides ; pharmacology ; Mitogen-Activated Protein Kinases ; metabolism ; NF-KappaB Inhibitor alpha ; NF-kappa B ; metabolism ; Ocimum ; chemistry ; Phosphorylation ; drug effects ; Plant Extracts ; pharmacology ; Protein Transport ; drug effects ; Proto-Oncogene Proteins c-akt ; metabolism ; RNA, Messenger ; genetics ; metabolism ; Reactive Oxygen Species ; metabolism ; Respiratory System ; cytology ; Water
8.Analysis on the hierarchical medical system of regional longitudinal medical consortium based on system dynamics
Zi-Xue SUN ; Jun-Rui LONG ; Guang-Feng DUAN ; Wen-Hua TIAN
Chinese Journal of Health Policy 2018;11(10):20-28
Objective: To analyze the change in community first diagnosis and two-way referral rate under the condition of the regional longitudinal medical consortium implementation in the country at large. According to the simulation results,some suggestions for hierarchical medical system were put forward. Methods : The community first diagnosis and two-way referral rates were determined using the literature meta-analysis, and were simulated under different conditions by means of the system dynamics. Results :The analysis results confirmed that simply increasing the financial subsidies can increase the community first diagnosis rate within a range from 0.08% to 0. 17%. For in case increases are made by the government in technical financial input to enhance the technical level of the community health service centers and in compensatory financial input to reduce the higher hospitals dependence on the light disease, results showed that the lower conversion rate increased within the range of 0.33%~0.45% in terms of the two-way referral rating. On the contrary, when technical financial input is increased to improve the technical level of community health service centers, the rate of change was in the range of 7.52% '- 12.61% - Conclusions : Firstly, the diversified strategy should be used to promote the achievement of graded diagnosis and treatment. Secondly, the Health Commission plays a key role in the promotion of themedical consortiumconstruction. Thirdly, medical insurance reimbursement differential strategy should be implemented and the supply-side reform should be strengthened. At last,attention should be paid to the rational development of existing referrals, especially the upper and lower ones.
9.Role of NADPH oxidase in high fat-induced oxidative stress injury in human umbilical vein endothelial cells
hu Wen XU ; zi Chun JIN ; long Xiao WANG ; lin Bo CONG ; Lan CUI
Chinese Pharmacological Bulletin 2017;33(11):1574-1578
Aim To explore the role of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in high fat-induced oxidative stress injury in human umbilical vein endothelial cells (HUVECs).Methods HUVECs were exposed to different concentrations of palmitic acid(0.1,0.2,0.4,0.8 mmol · L-1) for 24 h and different time points of 0.4 mmol · L-1 palmitic acid(0,12,24,48 h).Cell viability was measured by Cell Counting Kit 8,and the protein expression of NADPH oxidase subunits such as p22phox,p47phox,p67phox and gp91phox were determined by Western blot.The expression of reactive oxygen species (ROS) in HUVECs was detected by immunofluorescence.Results Cell proliferation rate of HUVECs stimulated by 0.4 mmol · L-1 palmitic acid for 24 h and 48 h was significantly reduced.In the next experiment,model group was accordingly set as HUVECs stimulated by 0.4 mmol · L-1 palmitic acid for 24 h.The expression of NADPH oxidase subunits such as p22phox,p47phox,p67phox and gp91phox significantly increased at 24 h and 48 h after 0.4 mmol· L-1 palmitic acid stimulation (P < 0.05),and the difference.between the 24 h group and the 48 h group was not significant (P > 0.05).The expression of ROS in HUVECs significantly increased at 24 h and 48 h after O.4 mmol · L-1 palmitic acid stimulation (P < 0.05),and the difference between 24 h group and 48 h group was not significant (P < 0.05).Compared with the model group (0.4 mmol · L-1 palmitic acid stimulation for 24 h),the NADPH oxidase inhibitor diphenyliodonium (DPI,10 μmol · L-1) pretreatment could significantly decrease the expression of ROS in vascular endothelial cells (P < 0.05).Conclusion Activated NADPH oxidase might play an important role in treatment of high fat-induced oxidative stress injury in vascular endothelial cells.
10.Relationship Between Helicobacter Pylori Infection and Carotid Intima Thickness by Meta–analysis
Xue-Shan DONG ; Zi-Long CHEN ; Wen-Xin FAN ; Ling CHEN
Chinese Circulation Journal 2018;33(4):366-370
Objectives: To investigate the relationship between helicobacter pylori (Hp) infection and carotid intima thickness. Methods: We searched the databases of Pubmed, Embase, CNKI, VIP and Wanfang for published studies on the relationship between Hp infection and carotid intima thickness in China and abroad. 2 reviewers independently identified and enrolled the articles, extracted specific data and assessed the quality of reference by Jadad scale. Meta-analysis was conducted by Stata 12.0 Software. Results: A total of 9 case-control studies were enrolled, all the reference quality scores≥4 which included 694 subjects with Hp positive and 676 with Hp negative. Meta-analysis indicated that carotid intima thickness was higher in Hp positive subjects than Hp negative subjects, standard mean difference (SMD)=0.803, 95% CI(0.686-0.919), P<0.05. Age related study presented that carotid intima thickness was higher in Hp positive subjects than Hp negative subjects at the age≥60 years'population, SMD=1.296, 95%CI (1.125-1.467), P<0.05; carotid intima thickness was also higher in Hp positive subjects than Hp negative subjects at the age<60 years'population, SMD=0.379, 95%CI (0.220-0.537), P<0.05. Conclusions: Hp infection has been related to the thickness of carotid intima and it could make carotid artery intima thickening.