1.Construction and expression of recombinant adenovirus containing secreted endostatin in liver stem cell as a vector for gene therapy.
Xiao-gang ZHONG ; Wu YIN ; Shun-rong HUANG ; Qian-zi QIN ; Wei MAI ; Fei LIU ; Rui HUANG ; Dan-rong LI
Chinese Journal of Hepatology 2009;17(8):628-629
Adenoviridae
;
genetics
;
Animals
;
Carcinoma, Hepatocellular
;
therapy
;
Cell Line
;
Endostatins
;
genetics
;
metabolism
;
Genetic Therapy
;
methods
;
Genetic Vectors
;
Green Fluorescent Proteins
;
metabolism
;
Humans
;
Liver
;
cytology
;
metabolism
;
Liver Neoplasms
;
therapy
;
Plasmids
;
genetics
;
Polymerase Chain Reaction
;
RNA, Messenger
;
genetics
;
metabolism
;
Rats
;
Recombination, Genetic
;
Stem Cells
;
cytology
;
metabolism
;
Transfection
2.A Family with Congenital Dysfibrinogenemia and Blood Transfusion.
Xiang-Cheng LIAO ; Shan-Shan ZHANG ; Zi-Ji YANG ; Chun-Li ZHU ; Hui-Ni HUANG ; Rui-Xian LUO ; Si-Na LI ; Hui-Qiong XIE ; Hai-Lan LI ; Zhu-Ning MO
Journal of Experimental Hematology 2023;31(5):1469-1474
OBJECTIVE:
To investigate a family with congenital dysfibrinogenemia, and analyze the risk of hemorrhage and thrombosis and blood transfusion strategies.
METHODS:
Prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT) of the proband and her family members were detected by automatic coagulometer, fibrinogen (Fg) activity and antigen were detected by Clauss method and PT algorithm respectively. Meanwhile, thromboelastometry was analyzed for proband and her family members. Then, peripheral blood samples of the proband and her family members were collected, and all exons of FGA, FGB and FGG and their flanks were amplified by PCR and sequenced to search for gene mutations.
RESULTS:
The proband had normal APTT and PT, slightly prolonged TT, reduced level of Fg activity (Clauss method). The Fg of the proband's aunt, son and daughter all decreased to varying degrees. The results of thromboelastogram indicated that Fg function of the proband and her family members (except her son) was basically normal. Gene analysis showed that there were 6233 G/A (p.AαArg35His) heterozygous mutations in exon 2 of FGA gene in the proband, her children and aunt. In addition, 2 polymorphic loci were found in the family, they were FGA gene g.9308A/G (p.AαThr331Ala) and FGB gene g.12628G/A (p.BβArg478Iys) polymorphism, respectively. The proband was injected with 10 units of cryoprecipitate 2 hours before delivery to prevent bleeding, and no obvious bleeding occurred during and after delivery.
CONCLUSION
Heterozygous mutation of 6233G/A (p.AαArg35His) of FGA gene is the biogenetic basis of the disease in this family with congenital dysfibrinogenemia.
Humans
;
Child
;
Female
;
Fibrinogen/genetics*
;
Pedigree
;
Afibrinogenemia/genetics*
;
Mutation
;
Blood Transfusion
3.Techniques and methods evaluation on pharmaceutical stability of liposomes
Zi-yuan HUANG ; Yu-qi SUN ; Hai-yang HU ; Rui ZHUANG ; Qi XU ; Da-wei CHEN
Acta Pharmaceutica Sinica 2016;51(3):356-
Liposomes as a drug carrier is easy to form aggregation and cause drug leakage in vitro. In addition, the degradation and elimination in vivo happens frequently to reduce its delivery activity. Development and application of liposomes are restricted by the instability. The appropriate techniques and methods are great important in the study of pharmaceutical stability of liposomes. In this paper, the techniques and methods are reviewed on pharmaceutical stability evaluation of liposomes, which was done from physical, chemical and biological stability for the difference in stability of liposomes. The research strategies for establishing the stability evaluation system and improving the value of liposomes have been discussed to make full therapeutic advantage of it.
4.Effect of C21 steroidal glycoside of Cynanchum auriculatum on liver and kidney fibrosis through TLR4 pathway.
Zi-Rui ZHUANG ; Ming-Liang WANG ; Yun-Ru PENG ; Ming-Qin SHEN
China Journal of Chinese Materia Medica 2021;46(11):2857-2864
The liver and kidney fibrosis model was established by thioacetamide(TAA) and unilateral ureteral obstruction(UUO) in SD rats. The rats were randomly divided into three groups: model group, low and high-dose groups of C21 steroidal glycosides of Cynanchum auriculatum. Another blank control group was set. Four weeks later, serum was taken to detect the biochemical indexes of liver and kidney function. Urine protein and urine creatinine were detected by kits. Liver and kidney tissue samples were stained with HE and Masson staining, and hydroxyproline content was detected. Western blot was used to detect expressions of fibrotic proteins, inflammatory factors and TLR4 signaling pathways, so as to observe the preventive and therapeutic effects of C21 steroidal glycosides from C. auriculatum on hepatic and renal fibrosis and explore its molecular mechanism. Four weeks later, serum biochemical results showed that liver and kidney functions were seriously damaged, and pathological sections showed that inflammatory cell infiltration, decrease of parenchymal cells, and increase of interstitial fibrosis in liver and kidney tissues. The results showed that low and high doses(150, 300 mg·kg~(-1)) of C21 steroidal glycosides could significantly reduce the collagen deposition and the pathological changes of liver and kidney fibrosis compared with the model group. At the same time, we found that the expression levels of TLR4 and MyD88 signaling pathway proteins were significantly increased in the liver and kidney tissues of the model group, and a large number of NF-κB signaling pathway proteins migrated into the nucleus. On the contrary, the expression levels of TLR4, MyD88 signaling pathway proteins and the nuclear migration of NF-κB were significantly inhibited in the low and high dose groups of C21 steroidal glycosides from C. auriculatum. Therefore, it was speculated that the mechanism of C21 steroidal glycoside for preventive and therapeutic effect on hepatic and renal fibrosis was related to inhibit TLR4/MYD88/NF-κB inflammatory pathway, thus preventing hepatic and renal fibrosis.
Animals
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Cynanchum
;
Fibrosis
;
Glycosides
;
Kidney/pathology*
;
Liver
;
NF-kappa B/genetics*
;
Rats
;
Rats, Sprague-Dawley
;
Toll-Like Receptor 4/genetics*
5.Progress in understanding hepatic fibrosis and renal fibrosis based on the gut-liver-kidney axis
Ming-liang WANG ; Yong-fang DING ; Xin YIN ; Jiu-zhen SHAO ; Zi-rui ZHUANG ; Ting ZHANG ; Peng-liang SU ; Yun-ru PENG
Acta Pharmaceutica Sinica 2021;56(1):9-20
Fibrosis is a pathological process of abnormal hyperplasia and excessive deposition of extracellular matrix during the process of repair after tissue and organ damage. Injury/inflammation caused by variously chronic diseases is a major trigger for fibrogenesis. Fibrosis of the liver and kidney is a common organ fibrosis. Recently, the intestinal microbiota has been shown to be extensively involved in the development of liver and kidney diseases, which may follow from changes in the intestinal microbial composition and intestinal integrity. This promotes the development of liver and/or kidney fibrosis through endocrine, cell signaling and other pathways. This paper reviews the research progress in understanding liver fibrosis and kidney fibrosis based on the gut-liver-kidney axis, which may be helpful for providing new strategies and theoretical basis for the diagnosis and treatment of hepatic and renal fibrosis.