2.The effects of functional magnetic resonance imaging on motor cortex function in patients with cerebral ischemic stroke
Zi-Qian CHEN ; Ping NI ; Hui XIAO ; You-Qiang YE ; Gen-Nian QIAN ; Xi-Zhang YANG ; Jin-Liang WANG ; Shang-Wen XU ; Mei NIE ; Yu SONG ; Biyun ZHANG ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(12):-
Objective To investigate the effects of functional magnetic resonance imaging (fMRI)with acute ischemic stroke (AIS) patients,and to evaluate the relationship between brain reorganization and motor recovery.Methods Nine AIS patients and 9 healthy volunteers were assessed by fMR1 during passive finger clenching at a pace of 1 Hz.The fMRI results were analyzed using SPM2 software.Lateral indices (LIs) and activated regions were calculated,and the relationship between LI and muscle strength was examined.Results In the control group,activation was observed in the contralateral sensorimotor cortex (SMC) and the bilateral supplementary area (SMA) during the passive movement.In the AIS group,similar results were recorded dur- ing unaffected hand movement,but the ipsilateral activation areas were greater than those on the eontralateral side during movement of the affected hand.LI results confirmed that movement of the affected hand mainly elici- ted activation in the ipsilateral hemisphere.Conclusion The different fMRI manifestations of patients and nor- mal subjects reflect brain compensation,and fMRI is valuable for studying the correlation between motor function and brain reorganization.
3.Study on concentration of nuorovirus genegroup II from environmental water.
Sha LIANG ; Guang-cheng XIE ; Zi-qian XU ; Jin-song LI ; Dan-tong LI ; Shao-long FENG ; Zhao-jun DUAN
Chinese Journal of Virology 2011;27(1):58-63
A new viral sampling concentration device was designed which was equipped with a new cationic filter membrane-Nanoceram suitable for field sampling. Norovirus Genegroup II was detected from environmental water with the aid of this device. The effects on virus recovery of prefiltration, various second-concentration methods, and different eluants were investigated through pre-experiment. The concentration optimized process, and the optimal concentration process were then determined. The results showed that the prefiltration had a profound effect on virus recovery, and two second-concentration method: PEG-NaC1 precipitation and celite adsorption, had almost the same concentration effects. The Na2 HPO4 solution of 0.15 mol/L was selected as the final eluant to elute the adsorbed Nuorovirus from the celite. The virus recovery of Nanoceram was determined to be 3.02%. Finally, successful detection of Norovirus GII in sewage from Yangqiao River, Fengtai District, Beijing was acheived. All these data had shown that the Naneceram filter concentration method could concentrate Norovirus from environmental water with a steady effects.
Filtration
;
instrumentation
;
methods
;
Fractional Precipitation
;
instrumentation
;
methods
;
Genotype
;
Norovirus
;
classification
;
genetics
;
isolation & purification
;
Rivers
;
virology
;
Water Microbiology
4.An investigation of bacterial ecology and analysis of bacterial resistance to antibiotics in a burn ward in Nanning district.
Hong-mian LI ; Zi-qian LIANG ; Da-en LIU ; Cheng-yue MENG
Chinese Journal of Burns 2005;21(2):107-110
OBJECTIVETo investigate the changes in the bacterial ecology and to analyze the bacterial resistance to antibiotics in a burn ward in Nanning district during the past 15 years, so as to provide reference to the clinical management of burn infection under subtropical climate.
METHODSFive thousand eight hundred and fifty-five strains of bacteria were isolated from the wounds and blood of 2269 burn patients admitted to our hospital from April of 1989 to March of 2004. Kiry-Bauer method was employed for the detection of antibiotic sensitivity test. The bacterial examination and bacterial resistance were analyzed in spans of every five years.
RESULTSBurn patients in our district were mainly infected by the gram negative bacilli (3559 strains, accounting for 60.79%), among which Pseudomonas aeruginosa, Enterobacter cloacae and Nitrate negative bacilli were major ones in every period. Gram positive cocci accounted for 33.99% (1990 strains), which ranked the second, among which Staphylococcus aureus, Staphylococcus epidermidis, and Coagulase negative staphylococci (MRCNS) were the most predominant ones. The bacterial resistance to multiple antibiotics, such as Gentamicin, third generation of Cephalosporin, and Norfloxacin showed a tendency of increase or maintained at high level while the incidence of resistance to Imipenem and Vancomycin was very low.
CONCLUSIONThe climate and the way of using antibiotics exerted direct effects on the status of the bacterial ecology and change in bacterial resistance to various antibiotics.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anti-Bacterial Agents ; pharmacology ; Burn Units ; Burns ; microbiology ; Child ; Child, Preschool ; Colony Count, Microbial ; Drug Resistance, Bacterial ; Gram-Negative Bacteria ; drug effects ; isolation & purification ; Gram-Positive Bacteria ; drug effects ; isolation & purification ; Humans ; Infant ; Microbial Sensitivity Tests ; Middle Aged ; Young Adult
5.Effect of flavonoids from Hippophae rhamniodes residues on blood lipoid metabolism and antioxidative activity in climacteric rats.
Xian-yan YANG ; Wei-jing QU ; Zi-liang XU ; Miu-Qian ; Li-xian ZHU ; Zhi-min XU
China Journal of Chinese Materia Medica 2006;31(13):1109-1112
OBJECTIVETo study the effect of flavonoids from seed residues of Hippophae rhamnoides (FH) on the lipid metabolism and antioxidative activity in climacteric rats.
METHODMenopausal rats with aging were used in this experiment. The rats were fed with FH by gastrogavage for 13 weeks. The effect of drug on the lipid metabolism and the antioxidative activity were observed after the rats were killed.
RESULTSerum total cholesterol was decreased significantly in rats fed with FH, T-AOC and SOD in serum and liver were significantly higher than those in rats fed with water, and at the same time MDA was lower than that in rats fed with water.
CONCLUSIONFH can improve the climacteric rats' lipid metabolism, and enhance the antioxidation in climacteric rats.
Animals ; Antioxidants ; pharmacology ; Cholesterol ; blood ; Climacteric ; blood ; Female ; Flavonoids ; isolation & purification ; pharmacology ; Hippophae ; chemistry ; Lipid Metabolism ; drug effects ; Liver ; metabolism ; Malondialdehyde ; blood ; metabolism ; Plants, Medicinal ; chemistry ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Seeds ; chemistry ; Superoxide Dismutase ; blood ; metabolism ; Triglycerides ; blood
6.Clinical study on the postburn change in the hypothalamus-pituitary-adrenal hormones in severely burned patients.
Hong-mian LI ; Zi-qian LIANG ; Zuo-jie LUO
Chinese Journal of Burns 2003;19(3):169-171
OBJECTIVETo investigate the postburn dynamic changes in the hypothalamus-pituitary-adrenal hormones in severely burned patients.
METHODSFifty burn patients were enrolled in the study. The plasma contents of total GC (cortisol), ACTH and aldosterone (ALDO) and urinary contents of 17-OHO and 17-KS were determined with radio-immunological assay (RIA) method after burn injury to compare with the normal values which were well established clinically.
RESULTSThe postburn plasma and urinary contents of the above indices were increased evidently with two peak values in shock and infectious stages, whilst the majority of he indices were lower than the normal values after 6 postburn weeks (PBWs). The values of these hormones were the lowest in dying patients. On the other hand, the values approached normal levels in those patients whose burn wounds were healing.
CONCLUSIONIncreases of the plasma and urinary levels of hypothalamus-pituitary -adrenal hormones in severely burned patients were constantly seen. Burn shock and infection seemed to be the two major factors in inducing postburn stress reaction in burn victims. Abrupt decrease of the hormone levels in plasma and or urine indicated adrenal failure predicting a poor prognosis of the burn patients.
Adrenal Cortex Hormones ; metabolism ; Adult ; Burns ; metabolism ; surgery ; Female ; Humans ; Hypothalamic Hormones ; metabolism ; Male ; Pituitary Hormones ; metabolism ; Shock, Traumatic ; metabolism ; surgery ; Time Factors ; Young Adult
7.Design of myocutaneous flap with color Doppler flow imaging technique for breast reconstruction in breast cancer after operation and radiotherapy.
Hong-mian LI ; Jian-hua GAO ; Feng LU ; Da-en LIU ; Zi-qian LIANG ; Li ZHANG
Chinese Journal of Surgery 2007;45(19):1338-1341
OBJECTIVETo investigate the method and effect of axial pattern myocutaneous flap in reconstructing breast by using color doppler flow imaging (CDFI) technique.
METHODSSuitable axial myocutaneous flaps were selected according to the character of the focus in 26 cases of breast cancer after operation and radiotherapy. All the axial pattern myocutaneous flaps were designed on the basis of traditional design method before operation; then, CDFI with high resolution was used to examine the starting spot, exterior diameter, trail and length of the myocutaneous flaps' major artery. The myocutaneous flaps were redesigned according to the results of CDFI and transferred to reconstruct the breasts. The results of operation and examination were investigated.
RESULTSAccording to the CDFI, only one thoracodorsal artery's blood current was slow, its wall was rough and presented with arteriosclerosis. The blood flow was fluent and the vessel wall was smooth with other supplying arteries in the flaps. And no embolism, sclerosis or absence of blood vessel was found. The starting spots, exterior diameters, trails and anatomic layers of the major supplying arteries of the flaps were displayed clearly with CDFI, in accordance with the results of operation. Twenty-one cases of latissimus dorsi myocutaneous flap, 4 cases of the contralateral transverse abdominis myocutaneous flap and 1 cases of the bilateral transverse abdominis myocutaneous flap were used in this group. The flaps survived and healed well, the breasts were reconstructed well with perfect appearance, shape and sensation.
CONCLUSIONSCDFI is a simple, visualized and noninvasive method for designing the axial pattern myocutaneous flap in breast reconstruction, it can provide more scientific and accurate evidence for preoperative determination of myocutaneous flap transplantation.
Adult ; Breast Neoplasms ; radiotherapy ; surgery ; Female ; Follow-Up Studies ; Humans ; Mammaplasty ; methods ; Mastectomy ; Middle Aged ; Surgical Flaps ; Treatment Outcome ; Ultrasonography, Doppler, Color ; Ultrasonography, Mammary ; methods
8.Experimental study on p53 gene mutation in keloid fibroblasts.
Wang LIU ; You-Hui JIANG ; You-Liang LI ; Zi-Hao LIN ; Hua JIANG ; Qian TAN ; Jian-Lin ZHANG ; Zi-Min WANG
Chinese Journal of Burns 2004;20(2):85-87
OBJECTIVETo explore the significance and the role of the p53 gene mutation in the exon 4 to 8 in keloid fibroblasts.
METHODSTissue samples from twelve patients with keloid and twelve hyperplastic scar respectively were harvested for in vitro culture of fibroblasts, and normal skin samples from the same patients were employed as the control. Polymerase chain reaction-based single-strained conformational polymorphism (PCR-SSCP) and DNA sequencing were employed to detect p53 gene mutations of the fibroblasts.
RESULTSThe points and frameshift mutations in the exon 4, 5, 6, 7 of p53 gene were identified in 9 of the 12 keloid tissue samples. No p53 gene mutation was detected in all hyperplastic scar and normal skin samples.
CONCLUSIONp53 gene mutation might play an important role in the formation and development of keloids.
Female ; Fibroblasts ; metabolism ; Genes, p53 ; Humans ; Keloid ; genetics ; Male ; Mutation
9.Pelvic Solitary Plasmacytoma: Computed Tomography and Magnetic Resonance Imaging Findings with Histopathologic Correlation.
Ying WANG ; Xiu Liang ZHU ; Mohamad Wasil PEEROO ; Zi Hua QIAN ; Dan SHI ; Shu Mei WEI ; Ri Sheng YU
Korean Journal of Radiology 2015;16(1):146-153
OBJECTIVE: To describe the imaging features of pelvic solitary plasmacytoma and to correlate them with the pathologic grade. MATERIALS AND METHODS: A retrospective study was performed on the imaging features of 10 patients with a histological diagnosis of pelvic solitary plasmacytoma. The imaging studies were assessed for bone expansion, cortical destruction, signal intensity/density of soft tissue mass and enhancement manifestations, which were then correlated to the pathologic grade. RESULTS: The imaging features of pelvic solitary plasmacytoma revealed 3 different types: multilocular type (n = 5), unilocular type (n = 2) and complete osteolytic destruction type (n = 3) on computed tomography and MRI. Pathologically, the tumors were classified into low, intermediate and high grades. Features such as multilocular change, perilesional osteosclerosis, slight expansion, local bone cortex disruptions and masses inside bone destruction, often suggest a low-grade solitary plasmacytoma; complete osteolytic destruction, huge soft tissue mass, and osseous defects imply a higher pathologic grade. CONCLUSION: Pelvic solitary plasmacytoma has various imaging manifestations, while a slight expansile osteolytic feature with multilocular change or homogeneous enhancement highly suggests its diagnosis. The distinctive imaging features of pelvic solitary plasmacytoma are well correlated to the pathologic grade.
Adult
;
Aged
;
Female
;
Humans
;
Magnetic Resonance Imaging
;
Male
;
Middle Aged
;
Neoplasm Grading
;
Pelvic Neoplasms/*pathology/radiography
;
Plasmacytoma/*pathology/radiography
;
Retrospective Studies
;
Tomography, X-Ray Computed
10.Characteristics analysis on major genes and the encoded proteins of human G9P8 rotaviruses LL52696 and LL52727.
Dan-Di LI ; Shu-Xian CUI ; Qing ZHANG ; Miao JIN ; Jie-Mei YU ; Dong-Liang ZHANG ; Zi-Qian XU ; Jing-Yu TANG ; Zhong Shan WANG ; Zhao-Yin FANG ; Zhao-Jun DUAN
Chinese Journal of Virology 2008;24(2):144-147
Two Rotavirus G9P[8] strains (LL52696 and LL52727) were recognized during a sentinel-based survey in Lulong, China. Phylogenetic analysis of the VP7 gene showed that both strains isolated constituted a divergent genetic cluster distinct from the other G9 strains isolated in China. Analysis of VP4, VP6, and NSP4 genes revealed that these strains were closely related to Lulong strains. We hold that two strains were reassortant between G9 and Lulong predominant strains.
Amino Acid Sequence
;
Antigens, Viral
;
chemistry
;
genetics
;
Base Sequence
;
Capsid Proteins
;
chemistry
;
genetics
;
Glycoproteins
;
chemistry
;
genetics
;
Humans
;
Phylogeny
;
Rotavirus
;
classification
;
genetics
;
Toxins, Biological
;
chemistry
;
genetics
;
Viral Nonstructural Proteins
;
chemistry
;
genetics