1.Comparison of liquid-based and conventional preparations in cytologic diagnosis of pancreatobiliary lesions.
Hong JIANG ; Zi-juan QI ; Ming DU ; Xu REN ; He-ming WU ; Lin LIU
Chinese Journal of Pathology 2013;42(4):269-270
Adult
;
Aged
;
Aged, 80 and over
;
Bile Duct Diseases
;
diagnosis
;
Bile Duct Neoplasms
;
diagnosis
;
Cholangiopancreatography, Endoscopic Retrograde
;
Cytodiagnosis
;
Cytological Techniques
;
methods
;
Female
;
Humans
;
Male
;
Middle Aged
;
Pancreatic Diseases
;
diagnosis
;
Pancreatic Neoplasms
;
diagnosis
2.Identification of Pterocephalus hookeri.
Xin-lu GUAN ; Yu-ning YAN ; Zi-he REN ; Tai-ming WEI ; Fu-yin LI ; Ying-shan ZHANG
China Journal of Chinese Materia Medica 2004;29(11):1027-1030
OBJECTIVETo study the identification method of Pterocephalus hookeri.
METHODThe microscopical, Physicochemical and TLC methods were used.
RESULT AND CONCLUSIONThe convenient and effective identification methods for P. hookeri were established, which provide basis for its quality standard and development.
Chromatography, Thin Layer ; Drugs, Chinese Herbal ; analysis ; Magnoliopsida ; anatomy & histology ; chemistry ; Pharmacognosy ; Plant Leaves ; anatomy & histology ; chemistry ; Plant Roots ; anatomy & histology ; chemistry ; Plants, Medicinal ; anatomy & histology ; chemistry ; Quality Control
3.Relationship between driver gene mutation and clinicopathological features in 300 cases of non-small cell lung cancer based on next generation sequencing
Qing-Jie HUANG ; Tian-Dong CHEN ; Hai-Rui CHEN ; Jia-Zi HE ; Guo-Zhong JIANG ; Wen-Cai LI ; Ren-Yin CHEN
Chinese Journal of Clinical and Experimental Pathology 2019;35(3):286-290
Purpose To explore the mutation characteristics of common driver genes in non-small cell lung cancer (NSCLC) and its relationship with clinicopathological features.Methods Next generation sequencing (NGS) was used to detect the mutations of common driving genes such as EGFR, KRAS, ALK, ROS1, BRAF, MET, RET and HER-2 in 300 paraffin-embedded NSCLC tissues. Results In 300 patients with NSCLC, the mutation rates of EGFR, KRAS, ALK, ROS1, BRAF, MET, RET, HER-2 were 52.00%, 10.33%, 6.67%, 1.67%, 3.67%, 3.33%, 1.00%, and 2.33%, respectively. A case of EGFR 21 exon L858 R mutation was combined with LINCO1446-EGFR gene fusion. EGFR 20 th exon C797 S and T790 M existed in cis or trans form and merged with EGFR sensitive mutations in 1 case each. 3 cases of EGFR gene point mutation was associated with MET gene copy number amplification. EGFR mutations were more commonly detected in non-smoking women with lung adenocarcinoma (P<0.05).KRAS mutations were more commonly found in smoking men (P<0.05). ALK mutations were associated with age (P<0.05), and more commonly noted in patients younger than 60 years.ROS1 fusion mutations were associated with gender (P<0.05), more commonly detected in women. BRAF, MET, RET, and HER-2 gene mutations were not associated with gender, age, smoking, histological type, and c TNM stage. Conclusion EGFR can coexist with other driver gene mutations. Gene mutations and clinicopathological features like gender, age, smoking, and histological types have corresponding links. The incidence of BRAF, MET, RET, and HER-2 mutations is low, and its clinical significance remains to be explored. Coexisting gene mutations and rare mutations discovered by NGS should be taken seriously.
4.Nucleocytoplasmic transport and neurodegenerative diseases
Zi-jian WANG ; Zi-heng LI ; Yi-kun CHANG ; Tian ZHANG ; Yuan-yuan REN ; Feng-qin HE
Acta Pharmaceutica Sinica 2021;56(5):1217-1228
Nucleocytoplasmic transport is the basic cellular activity of eukaryotic cells, which plays a role in cell physiological and pathological processes. A large amount of evidences indicate that impaired nucleocytoplasmic trafficking has emerged as a mechanism contributing to the pathology of neurodegenerative diseases. The regulation of nucleocytoplasmic transport is crucial to elucidate the pathogenesis and intervention in the neurodegenerative diseases. This article summarizes the evidences in disturbed nucleocytoplasmic transport of neurodegenerative diseases in the past two decades, further explores the directions and provides a theoretical basis for the pathogenesis and drug targets in neurodegenerative diseases.
5. Study on relationship of target organ injury of mechanism and "structure-effect-dose" of Hedysari Radix during radiotherapy-chemotherapy induced
Sha-Sha ZHAO ; Hai HE ; Zi-Yang WANG ; Yao-Ying XING ; Yuan REN ; Jing SHAO ; Sha-Sha ZHAO ; Hai HE ; Zi-Yang WANG ; Yao-Ying XING ; Jing SHAO ; Yuan REN ; Jing SHAO ; Jing SHAO
Chinese Pharmacological Bulletin 2024;40(2):371-380
Aim To explore the possible mechanism of "component-target-pathway" of Radix Hedysari against target organ damage caused by radiotherapy and chemotherapy, and to verify the " dose-effect" relationship of the main active components. Methods TCMSP, Uniprot, Swiss Target Prediction, GeneCards, Cytoscape, Omicshare and other platforms were used for network pharmacology analysis. Autodock, Pymol and Ligplot were used for molecular docking. The water extract of Radix Hedysari was used for animal experiment verification. The contents of eight main components were determined by HPLC. Results Four active components, eight key targets and four key pathways of Radix Hedysari were identified to resist the damage of target organs caused by radiotherapy and chemotherapy. Molecular docking showed that formononetin and quercetin had good binding activity with HSP90AA1, naringenin and MAPK3, and ursolic acid and TP53. Animal experiments showed that gastrointestinal factors MTL and VIP increased significantly, liver and kidney factors Cr, BUN, AST and ALT decreased significantly, inflammatory factor IL-10 increased significantly and TNF-a decreased significantly. The content of ononm was the highest (2 . 884 8 µg • g "
6. Cone-beam CT analysis of occlusal planes between different vertical skeletal types of adult class Ⅱ malocclusions in Shandong province
Tian-Zhen ZHANG ; Xin-Yu ZHENG ; Wei ZHANG ; Zi-Jing HE ; Ling-Zhi WANG ; Da-Peng REN ; Xiao YUAN ; Tian-Zhen ZHANG
Acta Anatomica Sinica 2022;53(2):210-216
[Abstract] Objective To investigate the cant of the occlusal planes in Chinese adult class Ⅱ malocclusions with hypodivergent, normodivergent, and hyperdivergent facial vertical patterns using cone-beam CT. Methods The descriptive, cross-sectional study evaluated 123 cephalogram from CBCT(CCB) of patients in Department of Orthodontics, the Affiliated Hospital of Qingdao University. According to cephalometric analysis, 89 Chinese adult class Ⅱ patients were divided into three groups according to the angle between FH-MP and the angle between SN-MP. In comparison, 34 Class I normodivergent malocclusions individuals were selected as the control group. Comparing the cephalometric result between 4 groups. Results As expected,the hyperdivergent group had steeper anterior occlusal planes(14. 73±3. 76) . and posterior occlusal planes(21. 42±5. 77)., there was a significant statistical difference between the hyperdivergent group and other 2 groups in class Ⅱ patients (P<0. 05). The height of maxillary second molar increased from high angle group to low angle group (P<0. 01). Conclusion There are different characters of the occlusal planes in varied vertical facial pattern. The inclinations of anterior and posterior occlusal planes in Chinese class Ⅱ hyperdivergent group were steeper than other 3 groups. The difference of posterior occlusal plane inclination is mainly caused by the height of maxillary second molar.
7.Traditional Uses, Phytochemistry, Pharmacology and Toxicology of Rhizoma phragmitis: A Narrative Review.
Yuan REN ; Ge-Dan CUI ; Li-Sha HE ; Huan YAO ; Chang-Yan ZI ; Yong-Xiang GAO
Chinese journal of integrative medicine 2022;28(12):1127-1136
Rhizoma phragmitis is a common Chinese herbal medicine whose effects are defined as 'clearing heat and fire, promoting fluid production to quench thirst, eliminating irritability, stopping vomiting, and disinhibiting urine'. During the Novel Coronavirus epidemic in 2020, the Weijing Decoction and Wuye Lugen Decoction, with Rhizoma phragmitis as the main herbal component, were included in The Pneumonia Treatment Protocol for Novel Coronavirus Infection (Trial Version 5) due to remarkable antiviral effects. Modern pharmacological studies have shown that Rhizoma phragmitis has antiviral, antioxidative, anti-inflammatory, analgesic, and hypoglycemic functions, lowers blood lipids and protects the liver and kidney. This review aims to provide a systematic summary of the botany, traditional applications, phytochemistry, pharmacology and toxicology of Rhizoma phragmitis.
Humans
;
Plant Extracts/pharmacology*
;
Rhizome
;
Drugs, Chinese Herbal/therapeutic use*
;
Antioxidants/therapeutic use*
;
Antiviral Agents/therapeutic use*
;
Medicine, Chinese Traditional
;
Phytochemicals/therapeutic use*
;
Ethnopharmacology
;
COVID-19 Drug Treatment
8.Changes in Cold and Hot Syndrome and Gastrointestinal Bacterial Community Structure in Mice by Intervention with Food of Different Nature.
Zhang-Cheng LIANG ; Wei-Xin LI ; Zhi-Gang HE ; Xiao-Zi LIN ; Xiang-Yun REN ; Xiao-Jie LIN
Chinese journal of integrative medicine 2020;26(6):448-454
OBJECTIVE:
To reveal the effect of foods with different natures on cold or hot syndrome and gastrointestinal bacterial community structure in mice.
METHODS:
Forty-five 6-week-old male ICR Kunming mice of clean grade were divided into 5 groups, 9 per group, including the control (CK), hot nature herbs (HM), Hong Qu glutinous rice wine (RW), tea rice wine (TW), and cold nature herbs (CM) groups. Distilled water or corresponding herbs were administered to mice (0.01 mL/g body weight) in the 5 groups by gastric infusion respectively, once daily for 28 d. Appearance, behavior, and serum biochemical indicators, including 5-hydroxytryptamine (5-HT), thyroid stimulating hormone (TSH), noradrenaline (NE), cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), the hot nature index, as well as the gastrointestinal bacterial community structure were analyzed in all groups after treatment.
RESULTS
After supplementation for 28 d, CM and TW mice showed different degrees of cold syndrome, and HM and RW mice showed different degrees of hot syndrome. Compared with the HM and RW mice, the TSH, NE, cAMP levels and hot nature indices in the CM and TW mice were significantly decreased and 5-HT and cGMP levels were significantly increased (P<0.05). There was no obvious change in appearance or behavior in CK mice. Results of clustering analysis showed that the gastrointestinal bacterial community structures were highly similar in TW and CM mice as well as in RW and HM mice, and that they were from the same branch, respectively, when the distance was 0.02. The key microbes associated with cold syndrome were Lachnospiraceae uncultured, Lactococcus, etc., and the key microbes associated with hot syndrome were S24-7 norank, Ruminococcaceae uncultured, etc. CONCLUSION: The interventions with different nature foods could change cold or hot syndrome in mice, leading to changes in gastrointestinal bacterial community structure.
9.Application of Optical Genome Mapping Technology in Detecting Complex Chromosomal Rearrangement
Shu-jing HE ; Zhi-qiang ZHANG ; Yi-juan HUANG ; Li-nan XU ; Yuan-qiu CHEN ; Cong FANG ; Zi REN
Journal of Sun Yat-sen University(Medical Sciences) 2023;44(6):943-948
ObjectiveTo investigate the application of optical genome mapping (OGM) technology in detecting complex chromosomal rearrangement. MethodsWe recruited five patients who were diagnosed as complex chromosomal rearrangement at the Reproductive Medicine Center of the Sixth Affiliated Hospital of Sun Yat-sen University from January 2022 to June 2023. They underwent OGM, nanopore sequencing and pre-implantation genetic testing (PGT). The results were compared with the results of karyotype and chromosomal microarray analysis (CMA)/ copy number variation sequencing (CNV-Seq). ResultsOGM could detect translocation, invert inversion, and triplet translocation, which were consistent with the results of OGM and CMA/ CNV-Seq. But OGM could not detect Robertsonian translocation. ConclusionBecause of its ultra-long reads, OGM realizes the detection across repetitive regions, and it has great advantages when applied in patients with complex chromosome rearrangement or uncertain karyotype analysis. It can accurately locate breakpoints.
10.Structures Characteristics and Bioactivity of Polysaccharide CALB-2 from Aurantii Fructus
Wu-jing WANG ; Yan-ni YANG ; Zi-he DING ; Ren-xing ZHONG ; Tian-yi XIA ; Shu-yuan LI ; Yi WANG ; Sheng-qing LI ; Zun-peng SHU
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(16):154-160
Objective:To isolate and purify a polysaccharide CALB-2 fraction from Aurantii Fructus,and analyze its basic chemical structure, morphological characteristics and bioactivity. Method:A refined CALB-2 was obtained from Aurantii Fructus by hot water extraction,then separated and purified by ion exchange resin,ion exchange agarose gel and propylene dextran gel to obtain homogeneous polysaccharide CALB-2. The molecular mass of CALB-2 was determined by high performance liquid chromatography (HPLC). Monosaccharide composition analysis of CALB-2 was conducted by methylation analysis and Smith degradation. Structural analysis and morphological characterization were conducted by infrared scanning (IR) and scanning electron microscopy (SEM) analysis. Antioxidant activity of CALB-2 was studied by using H2O2-induced cardiomyocyte oxidative damage model. Result:CALB-2 was a homogeneous polysaccharide and the molecular weight of CALB-2 was estimated to be 3.57×107 Da,which was proved to be a kind of highly branched acidic polysaccharides in IR analysis, methylation analysis and Smith degradation, mainly present in form of 1→3,4 bonds. Through SEM observations,we indicated that the molecular morphology of CALB-2 was amorphous solid. The