OBJECTIVE:To study the effects of Shenfu qiangxin(SFQX)pills on the expression of autophagy-associated pro-tein LC3b and pro apoptotic gene Bax in myocardial cells of rats with cardiorenal syndrome (CRS). METHODS:Rats were ran-domly divided into sham operation group(water),model group(water),positive control group(Captopril tablets 2.3 mg/kg)and SFQX pills high-dose,medium-dose and low-dose groups [13.2,6.6,3.3 g(crude drug)/kg],with 10 rats in each group. CRS mod-el was induced in those groups by abdominal-aortae-constriction+acute renal ischemia reperfusion injury except for sham operation group;and they were given relevant medicine intragastrically 8 week after operation,once a day,for consecutive 4 weeks. Plasma contents of Cr and ALD,the protein expression of LC3b and Bax in myocardial tissue of rats were detected 24 h after last medica-tion;ventricular index was calculated,and morphological change of myocardial tissue was observed. RESULTS:Compared with sham operation group,the plasma contents of Cr and ALD,ventricular index and the protein expression of LC3b in myocardial tis-sue increased significantly in model group (P<0.05 or P<0.01);and myocardial cell suffered from endochylema red deletion, myocardial cross striation disorder,intercellular space fibrosis aggravation and so on. Compared with model group,the plasma con-tents of Cr and ALD(except for positive control group)and the protein expression of LC3b and Bax in myocardial tissue decreased significantly in positive control group and SFQX pills high-dose group(P<0.05 or P<0.01);myocardial pathological change was improved;the ventricular index decreased significantly in SFQX pills low-dose and medium-dose groups (P<0.05). CONCLU-SIONS:SFQX pills can decrease the plasma contents of Cr and ALD,inhibit myocardial cell autophagy and apoptosis in CRS rats.

Objective: To establish the cardio-renal syndrome (CRS) model by coarctation of abdominal aorta (CAA) with renal ischemia reperfusion injury (RIRI), and to observe the mRNA expression of pro-renin receptor [(P)RR] in experimental rats. Methods: A total of 42 Wistar rats were randomly divided into 4 groups: Sham group, CAA group, RIRI group and CAA+RIRI group.n=10 in each group, 2 rats died during the modeling and all animals were treated for 16 weeks. Blood levels of BNP, creatinine (Cr), urea nitrogen (BUN), the activity of rennin, the contents of angiotensin-I (AT-I), AT-II and aldosterone were examined by laboratory test. The diastolic end inter-ventricular septum thickness (DEIVST), DELVPT, LVEF, ventricular weight index (VWI) and cardiac weight index were detected by small animal echocardiography. The histological changes of myocardium and kidney tissue were measured by HE staining, and the mRNA expressions of pro-renin receptor in myocardium and kidney tissues were measured by RT-PCR. Results: Compared with Sham group, blood levels of BNP were increased in the other 3 groups,P<0.05; compared with CAA group, CAA+RIRI group had increased levels of Cr and BUN,P<0.01; compared with Sham group and RIRI group, CAA+RIRI group showed increased blood level of aldosterone,P<0.05. Compared with CAA group, CAA+RIRI group presented increased rennin activity,P<0.05. Blood levels of AT-I and AT-II were not signiifcantly increased among 3 operation groups,P>0.05. Compared with CAA group, CAA+RIRI group had more obvious changes of DEIVST and LVEF,P<0.01. Compared with RIRI group, CAA+RIRI group had more obvious ventricular hypertrophy, higher VWI and cardiac weight index, allP<0.05. HE staining presented that CAA+RIRI group had broadening of myocardial cell bundle space, decreased left renal index, severe tubular atrophy and partial glomerular atrophy. RT-PCR demonstrated that compared with Sham group, the mRNA expressions of pro-renin receptor in myocardium and kidney tissues were decreased in the other 3 groups. Conclusion: Combined CAA+RIRI method may damage the cardial and renal tissues at the same time which was more severe than either CAA or RIRI. While CAA+RIRI model has better controllability and higher consistency that provides a methodological reference for pro-renin receptor in treating CRS in experimental rat’s model.

Objective To construct an evaluation index system of scientific and technological achievements transfor-mation in affiliated hospitals of universities and colleges based on a logical model,and to provide reference for scien-tific and systematic measurement of scientific and technological achievements transformation in affiliated hospitals of universities and colleges.Methods The first draft of the evaluation index system was drawn up by combing the re-search literature and laws and policies related to the evaluation of scientific and technological achievements transfor-mation in domestic hospitals,and two rounds of Delphi expert consultation were carried out to determine the evalua-tion index system.Results The expert positive coefficient of both rounds was 100%,the authority coefficient was 0.875 and 0.874,and Kendall's W coefficient increased round by round.Finally,an evaluation system covering 4 first-level indicators,12 second-level indicators and 44 third-level indicators of scientific and technological achieve-ments transformation in affiliated hospitals of colleges and universities was determined.Conclusion The evaluation in-dex system of scientific and technological achievements transformation in affiliated hospitals of colleges and universi-ties has certain reliability and scientificity,with obvious main characteristics,and has important guiding significance and practical value for promoting the optimization of scientific and technological achievements transformation in affiliated hospitals of colleges and universities and improving the transformation efficiency.

Objective To construct an evaluation index system of scientific and technological achievements transfor-mation in affiliated hospitals of universities and colleges based on a logical model,and to provide reference for scien-tific and systematic measurement of scientific and technological achievements transformation in affiliated hospitals of universities and colleges.Methods The first draft of the evaluation index system was drawn up by combing the re-search literature and laws and policies related to the evaluation of scientific and technological achievements transfor-mation in domestic hospitals,and two rounds of Delphi expert consultation were carried out to determine the evalua-tion index system.Results The expert positive coefficient of both rounds was 100%,the authority coefficient was 0.875 and 0.874,and Kendall's W coefficient increased round by round.Finally,an evaluation system covering 4 first-level indicators,12 second-level indicators and 44 third-level indicators of scientific and technological achieve-ments transformation in affiliated hospitals of colleges and universities was determined.Conclusion The evaluation in-dex system of scientific and technological achievements transformation in affiliated hospitals of colleges and universi-ties has certain reliability and scientificity,with obvious main characteristics,and has important guiding significance and practical value for promoting the optimization of scientific and technological achievements transformation in affiliated hospitals of colleges and universities and improving the transformation efficiency.

Objective To construct an evaluation index system of scientific and technological achievements transfor-mation in affiliated hospitals of universities and colleges based on a logical model,and to provide reference for scien-tific and systematic measurement of scientific and technological achievements transformation in affiliated hospitals of universities and colleges.Methods The first draft of the evaluation index system was drawn up by combing the re-search literature and laws and policies related to the evaluation of scientific and technological achievements transfor-mation in domestic hospitals,and two rounds of Delphi expert consultation were carried out to determine the evalua-tion index system.Results The expert positive coefficient of both rounds was 100%,the authority coefficient was 0.875 and 0.874,and Kendall's W coefficient increased round by round.Finally,an evaluation system covering 4 first-level indicators,12 second-level indicators and 44 third-level indicators of scientific and technological achieve-ments transformation in affiliated hospitals of colleges and universities was determined.Conclusion The evaluation in-dex system of scientific and technological achievements transformation in affiliated hospitals of colleges and universi-ties has certain reliability and scientificity,with obvious main characteristics,and has important guiding significance and practical value for promoting the optimization of scientific and technological achievements transformation in affiliated hospitals of colleges and universities and improving the transformation efficiency.

Objective To construct an evaluation index system of scientific and technological achievements transfor-mation in affiliated hospitals of universities and colleges based on a logical model,and to provide reference for scien-tific and systematic measurement of scientific and technological achievements transformation in affiliated hospitals of universities and colleges.Methods The first draft of the evaluation index system was drawn up by combing the re-search literature and laws and policies related to the evaluation of scientific and technological achievements transfor-mation in domestic hospitals,and two rounds of Delphi expert consultation were carried out to determine the evalua-tion index system.Results The expert positive coefficient of both rounds was 100%,the authority coefficient was 0.875 and 0.874,and Kendall's W coefficient increased round by round.Finally,an evaluation system covering 4 first-level indicators,12 second-level indicators and 44 third-level indicators of scientific and technological achieve-ments transformation in affiliated hospitals of colleges and universities was determined.Conclusion The evaluation in-dex system of scientific and technological achievements transformation in affiliated hospitals of colleges and universi-ties has certain reliability and scientificity,with obvious main characteristics,and has important guiding significance and practical value for promoting the optimization of scientific and technological achievements transformation in affiliated hospitals of colleges and universities and improving the transformation efficiency.

Objective To construct an evaluation index system of scientific and technological achievements transfor-mation in affiliated hospitals of universities and colleges based on a logical model,and to provide reference for scien-tific and systematic measurement of scientific and technological achievements transformation in affiliated hospitals of universities and colleges.Methods The first draft of the evaluation index system was drawn up by combing the re-search literature and laws and policies related to the evaluation of scientific and technological achievements transfor-mation in domestic hospitals,and two rounds of Delphi expert consultation were carried out to determine the evalua-tion index system.Results The expert positive coefficient of both rounds was 100%,the authority coefficient was 0.875 and 0.874,and Kendall's W coefficient increased round by round.Finally,an evaluation system covering 4 first-level indicators,12 second-level indicators and 44 third-level indicators of scientific and technological achieve-ments transformation in affiliated hospitals of colleges and universities was determined.Conclusion The evaluation in-dex system of scientific and technological achievements transformation in affiliated hospitals of colleges and universi-ties has certain reliability and scientificity,with obvious main characteristics,and has important guiding significance and practical value for promoting the optimization of scientific and technological achievements transformation in affiliated hospitals of colleges and universities and improving the transformation efficiency.

Objective To construct an evaluation index system of scientific and technological achievements transfor-mation in affiliated hospitals of universities and colleges based on a logical model,and to provide reference for scien-tific and systematic measurement of scientific and technological achievements transformation in affiliated hospitals of universities and colleges.Methods The first draft of the evaluation index system was drawn up by combing the re-search literature and laws and policies related to the evaluation of scientific and technological achievements transfor-mation in domestic hospitals,and two rounds of Delphi expert consultation were carried out to determine the evalua-tion index system.Results The expert positive coefficient of both rounds was 100%,the authority coefficient was 0.875 and 0.874,and Kendall's W coefficient increased round by round.Finally,an evaluation system covering 4 first-level indicators,12 second-level indicators and 44 third-level indicators of scientific and technological achieve-ments transformation in affiliated hospitals of colleges and universities was determined.Conclusion The evaluation in-dex system of scientific and technological achievements transformation in affiliated hospitals of colleges and universi-ties has certain reliability and scientificity,with obvious main characteristics,and has important guiding significance and practical value for promoting the optimization of scientific and technological achievements transformation in affiliated hospitals of colleges and universities and improving the transformation efficiency.

Objective To construct an evaluation index system of scientific and technological achievements transfor-mation in affiliated hospitals of universities and colleges based on a logical model,and to provide reference for scien-tific and systematic measurement of scientific and technological achievements transformation in affiliated hospitals of universities and colleges.Methods The first draft of the evaluation index system was drawn up by combing the re-search literature and laws and policies related to the evaluation of scientific and technological achievements transfor-mation in domestic hospitals,and two rounds of Delphi expert consultation were carried out to determine the evalua-tion index system.Results The expert positive coefficient of both rounds was 100%,the authority coefficient was 0.875 and 0.874,and Kendall's W coefficient increased round by round.Finally,an evaluation system covering 4 first-level indicators,12 second-level indicators and 44 third-level indicators of scientific and technological achieve-ments transformation in affiliated hospitals of colleges and universities was determined.Conclusion The evaluation in-dex system of scientific and technological achievements transformation in affiliated hospitals of colleges and universi-ties has certain reliability and scientificity,with obvious main characteristics,and has important guiding significance and practical value for promoting the optimization of scientific and technological achievements transformation in affiliated hospitals of colleges and universities and improving the transformation efficiency.

Objective To construct an evaluation index system of scientific and technological achievements transfor-mation in affiliated hospitals of universities and colleges based on a logical model,and to provide reference for scien-tific and systematic measurement of scientific and technological achievements transformation in affiliated hospitals of universities and colleges.Methods The first draft of the evaluation index system was drawn up by combing the re-search literature and laws and policies related to the evaluation of scientific and technological achievements transfor-mation in domestic hospitals,and two rounds of Delphi expert consultation were carried out to determine the evalua-tion index system.Results The expert positive coefficient of both rounds was 100%,the authority coefficient was 0.875 and 0.874,and Kendall's W coefficient increased round by round.Finally,an evaluation system covering 4 first-level indicators,12 second-level indicators and 44 third-level indicators of scientific and technological achieve-ments transformation in affiliated hospitals of colleges and universities was determined.Conclusion The evaluation in-dex system of scientific and technological achievements transformation in affiliated hospitals of colleges and universi-ties has certain reliability and scientificity,with obvious main characteristics,and has important guiding significance and practical value for promoting the optimization of scientific and technological achievements transformation in affiliated hospitals of colleges and universities and improving the transformation efficiency.