Objective] To discuss the relationship between CT perfusion imaging and TCM syndrome of liver cancer patients. [Methods]Through testing the Perfusion parameters of Lesions, surrounding liver tissue and liver tissue perfusion in the distance, we may explore the correlation among perfusion parameters, TCM syndrome of primary liver cancer, then explore the phase rule.[Results] There exists significant difference between perfusion parameters and TCM syndrome of liver cancer patients. Hepatic arterial perfusion(HAP), portal venous perfusion(PVP):deficiency syndrome of both liver and kidney yin>syndrome of heat-damp>syndrome of qi stagnation and blood stasis>syndrome of hepatic stagnation and spleen deficiency;Hepatic perfusion index(HPI):syndrome of hepatic stagnation and spleen deficiency>syndrome of qi stagnation and blood stasis>syndrome of heat-damp>deficiency syndrome of both liver and kidney yin. Child-Pugh classification: syndrome of hepatic stagnation and spleen deficiency(5.34 ±1.46),syndrome of qi stagnation and blood stasis(6.82±0.94),syndrome of heat-damp(8.34±1.12),deficiency syndrome of both liver and kidney yin(9.01±1.19).There exists significant difference between Child-Pugh classification and TCM syndrome of liver cancer patients(P<0.05). The result shows that a high positive correlation between AF, PI and Child-Pugh classification(P<0.05);There exists a negative correlation between PF and Child-Pugh classification(P<0.05).[Conclusion] CT perfusion parameters can be used as an objective indicator of middle-late stage of TCM syndrome of liver cancer patients.

· AlM: To investigate clinical therapeutic effect and safety of small incision extracapsular cataract extraction combined with intraocular lens implantation in blindness prevention and treatment.
· METHODS: Clinical data of 425 patients with cataract (425 eyes) were analyzed retrospectively, who received small incision extracapsular cataract extraction combined with intraocular lens implantation in the No.413 Hospital of Chinese PLA, with the help from “handicapped rehabilitation engineering in Dinghai District of Zhoushan City” from September 2013 to August 2014.Visual acuity before and after operation, average corneal curvature, corneal astigmatism and intraoperative and postoperative complications were compared statistically and analyzed emphatically.
·RESULTS: ln all of the 425 patients with cataract (425 eyes) , the preoperative best corrected visual acuity of 99 patients were less than 0.05, and the other 326 patients were 0.05 to 0.3.Classification of lens nucleus hardness:level Ⅲ, 63 cases; level Ⅳ, 257 cases; and level Ⅴ, 105 cases.The preoperative average corneal curvature and corneal astigmatism of all patients were 44.6 ±1.52D and 1.35±0.96D.All the 425 patients underwent small incision extracapsular cataract extraction combined with intraocular lens implantation.Postoperative follow-up of 3mo results: the best corrected visual acuity: 5 cases were less than 0.05 (blindness-free rate 98.8%);8 cases were 0.05 to <0.3 ( handicap-overcome rate 96.9%);42 cases were between 0.3 to <0.5;127 cases between 0.5 to 0.8 and 243 cases were ≥0.8.Postoperative average corneal curvature and cornea astigmatism were 44.5 ± 1.42D and 1.47 ±1.00D respectively. There were no statistically significant difference compared with preoperative condition.No severe complications such as infectious endophthalmitis or retinal detachment occurred, except 4 patients ( 0.9%) with posterior capsular rupture and 3 patients (0.7%) with Descemet’s membrane detachment during operation;and 18 patients (4.2%) with transient corneal edema and 4 patients (0.9%) with hyphema after operation.
· CONCLUSlON: lt is effective and safe to apply small incisionextracapsular cataract extraction combined with intraocular lens implantation in blindness prevention and treatment, and it gives excellent visual rehabilitation to cataract patients.Especially in those areas and hospitals having no condition of phacoemulsification, it’s a safe and effective alternative when carrying out the program of blindness prevention and treatment.

Objective To investigate surgical technique and clinical efficacy of Sky bone ex- pander kyphoplasty in the treatment of osteoporotic vertebral body compression fractures.Methods Eighteen cases with osteoporotic vertebral body compression fractures were treated with Sky bone expander kyphoplasty from August 2004 to November 2005.Under the local anesthesia,3.5-5ml of bone cements were injected into each pathologic vertebral body through unipedicle approach after reduction procedure was done with Sky bone expander.Results The postoperative follow-up ranged from 3 to 11 months, with an average of 4.5 months.Back pain was effectively relieved after the operation in all cases.No complications occurred.Conclusion The Sky bone expander kyphoplasty has the advantages of safe- ty,easy operation,minimal invasion,effective restoration of the vertebral body height and fast relief of pain.

OBJECTIVETo investigate the effects of polysaccharide 2-1 from Gastrodia elata (PGE2-1) on blood coagulation and thrombosis.

METHODClotting time (CT) and bleeding time (BT) of mice were measured by glass method and tail-cutting method. Bleeding capacity (A540) was measured by cutting tail in 5 min. Plama recalcificatic time (RT) were measured in mice. Platelet aggregation was caused by adenosine diphosphate (ADP). Activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) were measured by reagent boxes. During thrombosis in vitro, their lengths, wet and dry weights were measured by instrument; wet weights of arteriovenous experimental thrombosis were measured and the impressive rates were analyzed.

RESULTCT and BT of groups PGE2-1 (60, 120 mg x kg(-1)) were remarkably prolonged, and bleeding capacity (A540) were significantly increased (P < 0.05 or P < 0.01). RT of groups PGE2-1 (30, 60, 120 mg x kg(-1)) were remarkably prolonged, and platelet aggregation (PAG) were inhibited (P < 0.05 or P < 0.01). Human serous TT and APTT of groups PGE2-1 (10, 20, 40 mg x mL(-1)) were remarkably prolonged (P < 0.05 or P < 0.01), but the difference of effect on PT had no statistic significance. PGE2-1 (30, 60, 120 mg x kg(-1)) could make the mice obviously eliminate thrombus symptom and reduce the time of restoring independent activity (P < 0.05 or P < 0.01); thrombosis in vitro: Lengths, wet and dry weights of groups PGE2-1 (30, 60, 120 mg x kg(-1)) were significantly decreased (P < 0.05 or P < 0.01); wet weights of arteriovenous experimental thrombosis were dramatically decreased (P < 0.01), and impressive rates were respectively 32.5%, 49.0% and 61.5%.

CONCLUSIONPGE2-1 has remarkable effects of anticoagulation and antithrombosis, so it may be the main component of the isolation from G. elata in the field of antithrombosis.

Animals ; Anticoagulants ; isolation & purification ; pharmacology ; Blood Coagulation Tests ; Dose-Response Relationship, Drug ; Female ; Fibrinolytic Agents ; isolation & purification ; pharmacology ; Gastrodia ; chemistry ; Glycoproteins ; isolation & purification ; pharmacology ; Humans ; Male ; Mice ; Partial Thromboplastin Time ; Phytotherapy ; Plants, Medicinal ; chemistry ; Platelet Aggregation ; drug effects ; Prothrombin Time ; Random Allocation ; Rats ; Rats, Wistar ; Thrombin Time ; Thrombosis ; pathology ; prevention & control

OBJECTIVETo understand the epidemics and antigenic drift of influenza viruses in China from 2000 to 2001.

METHODSThe viruses were grown in embryonated hen eggs with 9 - 10 days old. The egg allantoic fluids with influenza viruses were used. Virion RNA was transcribed into cDNA by reverse transcriptase while cDNA amplified by PCR. Products of PCR were purified. RNA sequence analysis was then performed. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign (Version 1.03) and Editseq (Version 3.69) software.

RESULTSData from comparison of amino acid sequence on HA1 domain of HA protein molecule between H1N1 viruses isolated in 2001 and A/Shanghai/7/99 (H1N1) strain indicated that there was only one difference of amino acid located at 190 position (antigenic determinant D). However, phylogenetic analysis showed that there were two distinguishable genetically lineages of H1N1 viruses co-circulating in men in China in 2001. Two antigenically distinct genetic lineages of influenza B viruses were still existing in men in China. Most of influenza B viruses were Yamagata-like strain and there were two different amino acid sequences located at 197 and 199 position on HA1 domain of HA protein molecule, between Victoria-like virus isolated and B/Shandong/7/97 strain. When comparing amino acid sequences on HA1 protein domain of H3N2 viruses isolated in 2000 with those of A/Sydeney/5/97 (H3N2) virus, it was revealed that there were 7 - 8 differences of amino acid sequences between them. However, there were four differences related to amino acid sequences on HA1 protein domain between H3N2 viruses isolated in 2000 and in 2001. These results were further demonstrated by analysis of phylogenic tree.

CONCLUSIONSInfluenza was not prevalent in China from 2000 to 2001. The antigenic drifts of H3N2 and B/Victoria-like viruses occurred. Two antigenically distinct genetic lineages of influenza B viruses were still co-circulating in men in China. Two genetically distinct lineages of influenza A (H1N1) virus were also co-circulating in men in China.

Antigens, Viral ; genetics ; China ; epidemiology ; DNA, Viral ; genetics ; Female ; Genes, Viral ; genetics ; Genetic Variation ; Hemagglutinin Glycoproteins, Influenza Virus ; genetics ; Humans ; Influenza A virus ; classification ; genetics ; immunology ; isolation & purification ; Influenza B virus ; classification ; genetics ; immunology ; isolation & purification ; Influenza, Human ; epidemiology ; virology ; Male ; Orthomyxoviridae ; classification ; genetics ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; Sentinel Surveillance ; Sequence Analysis, RNA

Cardiomyocyte apoptosis leads to the functional incapacitation of myocardial plasmodium and plays an important role in the pathogenesis of heart failure transformed from compensable cardiac hypertrophy. Mitochondria are the main source of apoptosis-inducing molecule of various cells, and the role of caspartate-specific cysteinyl proteinase (caspase)-dependent mechanism has generally been accepted in the cardiomyocyte apoptosis. However, the significance of caspase-independent apoptosis-inducing factor (AIF) mechanism is not yet understood. The purpose of this study was to evaluate hypoxia-reperfusion-induced alterations of AIF mRNA and protein expressions in hypertrophic cardiomyocytes. Cardiomyocyte hypertrophy was produced by angiotensin II (0.1 mumol/L). The cells were cultured under the condition of hypoxia (95% N2 and 5% CO2; the O2 partial pressure was lower than 5 mmHg) for 8 h or 12 h (named as H8h and H12h groups, respectively), and then exposed to normal culture environment (named as H8h/R and H12h/R groups, respectively). Apoptosis was detected with Hoechst 33258 staining. The AIF mRNA and protein expressions were detected by RT-PCR and Western blot and quantified by gel scanning. The results were as follows: (1) The level of AIF mRNA expression was 0.29+/-0.08 (optical density, relative value) in the control group (hypertrophic cardiomyocytes cultured in normal environment). Compared with that in the control group, the levels of AIF mRNA expression were significantly higher in the groups of H8h and H12h (0.52+/-0.04 and 0.85+/-0.10), indicating that this effect was time-dependent. A further increase of AIF mRNA expression was observed in the groups of H8h/R (1.09+/-0.12) and H12h/R (1.41+/-0.23). (2) The level of AIF protein expression was 0.29+/-0.04 in the control group. Compared with that in the control group, the levels of AIF protein expression were significantly higher in the groups of H8h and H12h (2.07+/-0.15 and 3.12+/-0.19). The AIF protein expression was increased further in the groups of H8h/R (4.57+/-0.25) and H12h/R (5.71+/-0.27). The nuclear translocation of AIF protein was obvious only in the groups of H8h/R and H12h/R. (3) The expressions of AIF mRNA and protein were almost completely inhibited by AIF siRNA transfection. The siRNA transfection also reduced the apoptosis of hypertrophic cardiomyocytes in the groups of H8h/R and H12h/R but not in the groups of H8h and H12h. The apoptosis rate was significantly reduced by both AIF siRNA transfection and Ac-DEVD-cmk, an inhibitor of caspase-3. This reduction induced by two factors was more evident than that by one factor. (4) AIF nuclear translocation induced by hypoxia-reperfusion was not affected by inhibition of the activity of caspase-3. These data suggest that AIF plays a pivotal role in the apoptosis of hypertrophic cardiomyocytes induced by hypoxia-reperfusion.
Apoptosis ; Apoptosis Inducing Factor ; metabolism ; Cardiomegaly ; Cell Hypoxia ; Myocytes, Cardiac ; cytology ; Reperfusion Injury

Preparation of a poly (gamma-glutamic acid)-cisplatin conjugate was introduced and its in vitro antitumor effect was investigated. Poly (gamma-glutamic acids) was obtained by using fermentation methods. The hydrolyzed small molecular weight of poly (gamma-glutamic acids) was prepared by acid hydrolysis. The interaction between poly (gamma-glutamic acids) -cisplatin conjugate (PGA-CDDP) and DNA was investigated by PCR model. MTT assay was used to investigate the in vitro anticancer activity of the conjugate. Apoptosis assay of the conjugate was investigated by FCM assay and the in vivo toxicity was also proceeded. The results showed that the poly (gamma-glutamic acids) -cisplatin conjugate was obtained successfully and its yield is 10% - 12%. It has obvious antitumor effects on human liver tumor BEL7404 cells, human lung tumor H446 cells and human colon tumor RKO cells. At the same time, it also has apoptosis effects on the three kinds of tumor cell lines. The in vivo toxicity of PGA-CDDP was examined in normal mice and the results showed that the in vivo toxicity of this conjugate was significantly lower than that of free CDDP. In conclusion, the poly (gamma-glutamic acids) -cisplatin conjuate could be used as a potential clinic antitumor drug. The poly (gamma-glutamic acids) obtained by fermentation can be used as a valuable drug carrier system.
Animals ; Antineoplastic Agents ; administration & dosage ; pharmacology ; Apoptosis ; drug effects ; Cell Survival ; drug effects ; Cisplatin ; administration & dosage ; pharmacology ; Drug Carriers ; Female ; Fermentation ; Hydrogen-Ion Concentration ; Male ; Mice ; Polyglutamic Acid ; administration & dosage ; pharmacology

OBJECTIVETo develop a human ovarian carcinoma SKOV3 model in severe combined immunodeficiency (SCID) mouse and to study its biologic characteristics.

METHODSHuman ovarian carcinoma SKOV3 cells were injected intraperitoneally into female SCID mouse to establish a transplantation model of human ovarian carcinoma. The biological characteristics, metastasis and morphology of transplanted tumors were studied.

RESULTAll tumors grew progressively with no sign of regression. The tumor cells spread around the peritoneal cavity and mainly on the diaphragm, mesentery, peritoneum and around the liver, which was confirmed by histopathology. The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.

CONCLUSIONAn intraperitoneal transplantation model of human ovarian carcinoma SKOV3 in SCID mice has been developed successfully, which can simulate the biological behavior of peritoneal metastasis of human ovarian carcinoma.

Animals ; Disease Models, Animal ; Female ; Humans ; Mice ; Mice, SCID ; Neoplasm Transplantation ; Ovarian Neoplasms ; pathology ; ultrastructure ; Peritoneal Neoplasms ; secondary ; Transplantation, Heterologous

BACKGROUNDTo understand the antigenic and genetic characteristics of Victoria like strain of influenza B virus isolated recently and to provide a scientific evidence for influenza surveillance and monitoring of influenza epidemic in future.

METHODSViruses were passed in embryonated hen eggs and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit. Afterwards RNA sequence analysis was performed by dideoxynucleotide chain termination and a cloning method. Finally, phylogenetic analysis of the sequencing data was performed with MegAlign.

RESULTSB/Sichuan/63/2001 and B/Zhejiang/2/2001 viruses were antigenically different from B/Shandong/7/97 strain. The substitution of nucleotide sequences of HA1genes of them compared with those of B/Shandong/7/97strain resulted in the change of amino acid sequences in antigenic determinants on HA1 protein domain. The phylogenetic analysis also indicated that strains isolated recently were genetically different from B/Shandong/7/97/strain. However, there was neither differences on the antigenicity nor genetic partern between these two isotates.

CONCLUSIONSThe antigenic drift of Victoria-like strain of influenza B virus isolated recently in China has further occurred.

Amino Acid Sequence ; Antigenic Variation ; China ; Epitopes ; Genetic Variation ; Hemagglutinin Glycoproteins, Influenza Virus ; genetics ; immunology ; Humans ; Influenza B virus ; genetics ; immunology ; Influenza, Human ; virology ; Molecular Sequence Data ; RNA, Viral ; analysis ; Sequence Analysis, RNA

Human glutamate decarboxylase 65(hGAD65) is an enzyme that catalyzes the transformation of L-glutamic acid into ?-aminobutyric acid.It has been found that Type 1 diabetes mellitus(T1DM)is an autoimmune disease,in which pancreatic islet ?-cells are destroyed due to immune response mediated by autoantigen.hGAD65 is considered as a key autoantigen of the autoimmune response,so anti-hGAD65 antibody(hGAD65-Ab) is the most effective and specific immune marker for T1DM diagnosis,and hGAD65 can be used to detect hGAD65-Ab in serum of T1DM patients.The hGAD65 gene was cloned into pET32a(+),then the recombinant plasmid with hGAD65 was transformed into E.coli BL21(DE3) and expressed by IPTG induction.The fusion protein containing thioredox,hexahistidine and hGAD65(Trx-hGAD65) was mostly insoluble,but the band of soluble Trx-hGAD65 could also be detected by SDS-PAGE,and it was a great improvement compared with the results reported.Trx-hGAD65 was isolated from lysate and purified by immobilized metal ion affinity chromatography(IMAC).After enterokinase digestion and IMAC purification,hGAD65 with high purity was obtained.Detection of thin-layer chromatography(TLC) showed that both Trx-hGAD65 and hGAD65 had enzymatic activity,whereas Trx-hGAD65 had better stability.Furthermore,it was confirmed that Trx-hGAD65 was able to conjugate with hGAD65-Ab in the serum of T1DM patients by ELISA assay.In conclusion,Trx-hGAD65 instead of hGAD65 can be used for T1DM diagnosis,and its application in prophylaxis and therapy of T1DM is expectable.