Objective To discuss the relationship between anxiety and nutriture of postoperative patients with laryngeal carcinoma.Methods SAS was used to investigate and analyze 150 patients 24-48 hours after laryngectomy of their anxiety,and 78 cases were chosen for further study and divided into the mile group,moderate group and severe group according to their anxiety degree.NRS2002 was used to evaluate their nutriture a day before and 10 days after operation,and lymphocyte count,stitches removal time,extubation time and hospitalization time after surgery were compared.Results There were statistically significant differences of BMI,ALB,lymphocyte count 10 days after surgery,stitches removal time,extubation time and hospitalization time after surgery among three groups (F =58.540,88.062,61.581,113.31,23.77,37.10,respectively; P ＜ 0.01).Patients with higher degree of anxiety had worse nutriture and worse prognosis.Conclusions There is close relationship between anxiety and nutriture in postoperative patient with laryngeal carcinoma.Patients with higher level of anxiety had worse nutriture,worse wound healing and longer hospitalization time,alleviating their anxiety can help improve their nutriture,and psychological intervention can improve their prognosis.

Objective To investigate the correlative factors between anxiety and prognosis of sudden deafness. Methods To analyze the changes of sudden deafness inpatients' anxiety by using homemade questionnaires and Zung anxiety self-evaluation chart (SAS) ,and to analyze the relationship of sudden deafness with anxiety and prognosis factors. Results 84 out of 105 patients (80%) had various degrees anxiety, deafness occurred most frequently whose ages were from 21 to 39 and from 40 to 59,the incidence rate were 33.33% and 50% respectively; Anxiety was associated with accompanying symptoms of tinnitus and vertigo; After 10 - 14 days regular treatment, the results demonstrated that the total effective rate of non-anxiety patients was 71.43% and the anxiety patients' was 54. 76%. Conclusions Sudden deafness patients show significant anxiety. Psychological counseling and health education were applied to these patients aiming directly at anxiety emotion, these actions are hopeful to improve the treatment effect.

ObjectiveOur recent study has demonstrated that extracellular acidification promotes lipid accumulation in macrophages via the activation of acid sensing ion channel 1 (ASIC1), but the underlying mechanism remains unclear. This study aims to explore the effect of extracellular acidification on macrophage lipophagy and the underlying mechanism. MethodsRAW264.7 macrophages were incubated with 25 mg/Lox-LDL in a pH 6.5 culture medium for 24 h to build macrophage-derived foam cell models induced by extracellular acidification. Then, RAW264.7 macrophages were cultured in the acidic medium of pH 6.5 with or without PcTx-1 (ASIC1 specific blocker, 10 μg/L) or Nec-1 (RIP1 specific inhibitor, 20 μmol/L) for 24 h, intracellular lipid accumulation was observed by oil red O staining. The expressions of total ASIC1, plasma membrane ASIC1, RIP1, p-RIP1 Ser166, TFEB, p-TFEB Ser142, LC3 and p62 were measured by Western blot. The co-localization of lipids (indicated by Bodipy) with LC3II (autophagosomes) and LAMP1 (lysosomes) was analyzed by a confocal laser scanning microscopy, respectively. Morphological changes of lipophagy in the cells were observed by using transmission electron microscopy. ABCA1-mediated cholesterol efflux was determined by cholesterol fluorescence kits. ResultsCompared with pH 7.4+ox-LDL group, the intracellular lipid accumulation in the pH 6.5+ox-LDL group was significantly increased. Meanwhile, the expressions of plasma membrane ASIC1, p-RIP1 Ser166, p-TFEB Ser142, and p62 proteins were elevated significantly, while LC3II protein level and LC3II/LC3I ratio were decreased. Accordingly, compared with pH 7.4+ox-LDL group, the macrophage lipophagy of the pH 6.5+ox-LDL group was inhibited as indicated by the decreased localization of lipid droplets with LC3 and LAMP1, a decrease in the number of lipophagosomes as well as an increase in lipid droplets. Furthermore, ATP binding cassette transporter A1 (ABCA1)-dependent cholesterol efflux from the macrophages of pH 6.5+ox-LDL group reduced dramatically. However, these above effects of extracellular acidification on RAW264.7 macrophages were abolished by PcTx-1 and Nec-1, respectively. ConclusionThese findings suggest extracellular acidification promotes the phosphorylation of TFEB at Ser142 via activating ASIC1/RIP1 pathway, thereby impeding lipophagy in RAW 264.7 macrophages, and that ASIC1 may be a new potential target for preventing aberrant lipid accumulation diseases including atherosclerosis.