ObjectiveTo explore relation of muscle tension on recovery of upper limbs motor function of patients with hemiplegia after stroke.Methods102 hemiplegia patients were divided into 4 groups according to the degree of muscle tension,and treated with routine rehabilitation training. After one month,upper limbs motor ability and activities of daily living (ADL) of patients were assessed.ResultsAfter treatment,upper limbs motor ability and ADL of all patients were raised,but patients with light muscle tension had a better effect compared with those with serious muscle tension (P<0.01).ConclusionExcessive muscle tension will block the motor functional recovery of patients with hemiplegia after stroke.

Stroke is one of the most common diseases among middle and senior - aged people,and depressive disorder,which hinders severely recovery and prognosis,is one of the complications of stroke.This paper analyzes the efficiency of social intervention mode on patients with post - stroke depressive disorder,and comes up with the conclusion that social intervention mode can not only help to ameliorate the occurrence of post - stroke depressive disorder,but also promote the recovery of patients' nervous function.Therefore,the social intervention mode can be regarded as an effective means for medical treatment.

· AlM: To investigate clinical therapeutic effect and safety of small incision extracapsular cataract extraction combined with intraocular lens implantation in blindness prevention and treatment.
· METHODS: Clinical data of 425 patients with cataract (425 eyes) were analyzed retrospectively, who received small incision extracapsular cataract extraction combined with intraocular lens implantation in the No.413 Hospital of Chinese PLA, with the help from “handicapped rehabilitation engineering in Dinghai District of Zhoushan City” from September 2013 to August 2014.Visual acuity before and after operation, average corneal curvature, corneal astigmatism and intraoperative and postoperative complications were compared statistically and analyzed emphatically.
·RESULTS: ln all of the 425 patients with cataract (425 eyes) , the preoperative best corrected visual acuity of 99 patients were less than 0.05, and the other 326 patients were 0.05 to 0.3.Classification of lens nucleus hardness:level Ⅲ, 63 cases; level Ⅳ, 257 cases; and level Ⅴ, 105 cases.The preoperative average corneal curvature and corneal astigmatism of all patients were 44.6 ±1.52D and 1.35±0.96D.All the 425 patients underwent small incision extracapsular cataract extraction combined with intraocular lens implantation.Postoperative follow-up of 3mo results: the best corrected visual acuity: 5 cases were less than 0.05 (blindness-free rate 98.8%);8 cases were 0.05 to <0.3 ( handicap-overcome rate 96.9%);42 cases were between 0.3 to <0.5;127 cases between 0.5 to 0.8 and 243 cases were ≥0.8.Postoperative average corneal curvature and cornea astigmatism were 44.5 ± 1.42D and 1.47 ±1.00D respectively. There were no statistically significant difference compared with preoperative condition.No severe complications such as infectious endophthalmitis or retinal detachment occurred, except 4 patients ( 0.9%) with posterior capsular rupture and 3 patients (0.7%) with Descemet’s membrane detachment during operation;and 18 patients (4.2%) with transient corneal edema and 4 patients (0.9%) with hyphema after operation.
· CONCLUSlON: lt is effective and safe to apply small incisionextracapsular cataract extraction combined with intraocular lens implantation in blindness prevention and treatment, and it gives excellent visual rehabilitation to cataract patients.Especially in those areas and hospitals having no condition of phacoemulsification, it’s a safe and effective alternative when carrying out the program of blindness prevention and treatment.

ObjectiveTo explore the best applying time and effect of baclofen on recovery of motor function of stroke patients with hemiplegia.Methods83 stroke patients with hemiplegia were divided into group A and group B according to the degree of muscle tension, every group was divided into the treatment group and control group. Patients of the four groups were treated with routine rehabilitation training, and baclofen was added to the patients of two treatment groups. Motor ability and activities of daily living (ADL) of patients were evaluated before and after one month treatment.ResultsAfter one month treatment, motor ability and ADL of all patients were improved, but in group A, there were not significant differences existed between treatment group and control group (P>0.05), while, there was a significant difference existed in group B(P<0.01).ConclusionBaclofen is necessary to stroke patients with hemiplegia only when the muscle tension increased obviously (≥Asworth 3).

Objective To explore the effective method for in vitro culture of the dendritic cells(DCs) and the specific anti-leukemic cell effect mediated by dendritic cells pulsed with acute myelogenous leukemia antigen. Methods Bone marrow mononucleas cells (BMMNCs) isolated from AML patients were induced to undergo differentiation with 500 ng/ml A23187 and pulsed with AML antigen. After 96 h, DCs and T cells were co-cultured for 5 to 7 days. The cytotoxic activity of cytotoxic T lymphocyte(CTL) to AML were detected with MTT colorimetry. Results BMMNCs isolated from AML patients treated with 100 ng/ml rhGM-CSF in combination with 500 ng/ml A23187 for 96 h exhibited typical morphology of DCs with rapidly increased expression of CD1a and CD83 (P<0.01). Dendritic cells pulsed with acid eluted tumor antigen peptides group displayed the strongest cytotoxic activity of CTL to AML. There was a significant difference between two groups(P<0.01). Conclusion BMMNCs isolated from AML patients can be successfully induced to DCs with rhGM-CSF and A23187 and dendritic cells pulsed with acid eluted tumor antigen peptides group had the strongest cytotoxic activity of CTL to AML.

Objective To investigate the influence of propofol on the induction of inducible nitric oxide synthase (iNOS) expression in spinal cord after spinal cord injury (SCI) in rats. Methods SCI model was reproduced according to Allen′s method with modification. Seventy-four SD rats were randomly divided into three groups: normal group (n=4), SCI with propofol treatment group (n=35), and SCI with saline treatment group (n=35). Immunohistochemistry and Western blot were employed to detect iNOS expression in spinal cord at different time (1h, 3h, 8h, 1d, 3d, 7d, 14d). Results The results showed that iNOS expression in spinal cord of propofol treatment group was significantly decreased compared with that of saline treatment group (P

Objective To study the clinical, neuro-electrophysiology features of Charcot-Marie-Tooth disease type 1A (CMT1A) and its gene mutation analysis.Methods 9 members of the family with CMT1A underwent detailed clinical examinations and gene mutation analysis was carried out in 7 of them. The probands accepted electromyography and nerve, muscle biopsy.Results Five patients of the family were attacked and consistent with autosomal dominant inheritance type. Except one asymptomatic patient, age at onset was in the first or second decade. The clinical features were slowly progressive distal muscle weakness, atrophy and end-brush form sensory decrement, diminished or absent tendon reflexes, foot deformity(pes cavus).The probands showed highly decreased sensory and motor conduction velocities. Gene mutation analysis showed large fragment tandem duplication containing peripheral myelin protein 22(PMP22) gene in all four patients out of the seven family members who attended the gene diagnosis.Conclusion CMT1A is the most common form of CMT. The disease usually begins in childhood or adolescence. Clinical featurs include progressive distal muscle weekness and atrophy, diminished or absent tendon reflexes. The motor nerve conduction velocity is slowed below the limit of 38 m/s. Tandem duplication on chromosome 17p11.2, encompassing the PMP22 gene is the main mutation type of CMT1A.

Objective To investigate the mutation of Cx32 gene,clinical and electrophysiological features of Chinese patients with Charcot-Marie-Tooth(CMT) disease.Methods 24 CMT probands were selected for Cx32 mutation screening after the exclusion of the CMT1A 1.5 Mb duplication and male-to-male transmission.The motor and sensory nerve conduction studies were performed in all probands and most of their affected family members to establish the clinical CMT1,CMT2 or CMT intermediate diagnosis.The presence of mutations in the coding region of Cx32 was detected by single-strand conformation polymorphism analysis combined with direct sequencing.Results It was found 7 different point mutations in the coding region of Cx32 in 1 sporadic CMT1 patient and 6 X-linked inherited families,including 4 families with CMT1 diagnosis and 2 families with CMT intermediate diagnosis.There were 20 male CMTX patients,6 female CMTX patients and 12 asymptomatic female carriers among 38 family members bearing Cx32 mutation.All of the 26 patients were mildly to moderately affected clinically.Conclusions Seven different Cx32 point mutations were detected and the percentage of Chinese CMT families with Cx32 mutation is about 10% in our study.The inheritance model of CMT secondary to Cx32 mutation could be X-linked dominant,X-linked recessive or sporadic.Male patients are usually more severly affected than females with slower nerve conduction velocities.Cx32 mutation screening should be firstly performed in those CMT families without male-to-male transmission and CMT1A duplication.

Objective To investigate the mechanism of repression of testosterone biosynthesis in rats exposed to dibutyl phthalate (DBP). Methods Immature male rats were randomly divided into 5 groups involving 4 experimental groups and one control group. Animals were administered orally either corn oil only (vehicle control) or DBP (0.25, 0.50, 1.00, 2.00 g/kg) for 30 days. After 30 days exposure, the half of animals were sacrificed with testis, epididymis and adrenal selected and weighed. The ratio of organ to body weight were calculated and histopathological examination were examined. Testosterone (T), luteinizing hormone (LH), 17?-estradiol (17?-E2) and corticosterone (CORT) were detected by radioimmunoassay. After 15 days, the rest of animals were sacrificed and above-mentioned indexes were measured. Results The ratio of testis and epididymis to body weight reduced significantly at doses of 0.50 g/kg DBP and above. The testicle atrophy was observed, germinal epithelium became thin and the amount of reproductive cells obviously decreased. All of those were not repaired in convalescence stage. In above 1.00 g/kg DBP groups, the level of T, LH and 17?-E2 decreased (P

Child, Preschool ; Facial Asymmetry ; congenital ; pathology ; Growth Disorders ; congenital ; pathology ; Humans ; Hypertrophy ; congenital ; Infant ; Male