1.Successful treatment of post-transplant lymphoproliferative disorder in allogeneic hemopoietic stem cell transplant recipient for severe aplastic anemia
Hui-Lan LIU ; Zi-Min SUN ; Liang-Quan GENG ;
Chinese Journal of Organ Transplantation 2005;0(10):-
Objective To study early diagnosis and treatment of post transplant lymphoprolifer- ative disorder in allogeneic hemopoietic stem cell transplant recipients.Methods A 16 years old patient with severe aplastic anemia received HLA-mismatched sibling allogeneic hemopoietic stem cell trans- plant after conditioning with cyclophosphamide/antithymocyte globulin/methylprednisolone(CY/ ATG/MP)regimen.Results On the day 72 posttransplantation,he developed lymphoproliferative disorder.After withdrawal of CsA,he was treated with methylprednisolone,intravenous immune globulin and IFN alpha,and recovered completely from PTLD.Conclusions PTLD is a rare and fatal complication of both solid-organ and hemopoietic stem cell transplantation.Surveillance for PTLD by PCR for circulating EBV-DNA may be appropriate in high risk settings.Early diagnosis,immunosup- pression therapy reduction or even withdrawal in time is important.
3.Not Available.
Zi hao LIU ; Shu quan ZHAO ; Jian yi CHEN ; Wei nian DENG ; Qing SHI ; Yi wu ZHOU
Journal of Forensic Medicine 2021;37(5):718-720
4.Analysis of the therapeutic effect and safety of diagnosis and treatment regimen in Chinese adult patients with acute lymphoblastic leukemia--the comparative study of one single centre.
Juan TONG ; Zi-min SUN ; Hui-lan LIU ; Liang-quan GENG ; Dong-yue CUI ; Xing-bing WANG ; Kai-yang DING ; Bao-lin TANG ; Xin LIU ; Wei-bo ZHU
Chinese Journal of Hematology 2013;34(4):349-352
5.Comparison of curative efficacy after G-CSF-mobilized sibling HLA-matched peripheral blood hematopoietic stem cell transplantation versus that combined with BMT for patients with hematologic malignancies in a single center.
Fu-Peng REN ; Hiu-Lan LIU ; Zi-Min SUN ; Liang-Quan GENG ; Xing-Bing WANG ; Kai-Yang DING
Journal of Experimental Hematology 2011;19(2):404-409
This study was aimed to retrospectively analyze and compare the clinical curative efficacy of patients with hematologic malignancies after G-CSF-mobilized sibling HLA-matched (sm) peripheral blood hematopoietic stem cell transplantation (sm-allo-PBHSCT) and sm-allo-PBHSCT combined with bone marrow transplantation (BMT). 100 patients received sm-allo-HSCT in a single center from October 2001 to October to 2010, included 38 patients received sm-allo-PBHSCT and 62 patients received sm-allo-PBHSCT combined with BMT. The myeloablative or reduced intensity conditioning regimens were chosen according to the condition of patients. All patients received standard cyclosporine (CsA) and mycophenolate mofetil (MMF) as prophylaxis for GVHD. The results showed that the rapid hematopoietic reconstitution was observed in all patients. The median time of ANC ≥ 0.5 × 10(9)/L in both groups were 12 days, the median time of platelet count ≥ 20 × 10(9)/L was 15 days in sm-allo-PBHSCT group and 16 days in sm-allo-PBHSCT + BMT group. The incidence of acute GVHD, acute GVHD of III-IV grade and chronic GVHD in sm-allo-PBHSCT and sm-allo-PBHSCT + BMT groups were 37.1% and 34.2%, 7.89% and 8.06%, 36.11% and 41.38% respectively, there were no statistical differences. The relapse rates were similar in two groups (sm-allo-PBHSCT 13.16% vs sm-allo-PBHSCT + BMT 12.9%). The 3-year disease-free survivals in sm-allo-PBHSC and sm-allo-PBHSCT + BMT groups were 57.1 ± 8.7% and 61.3 ± 6.4% respectively (p = 0.852). The 2-year overall survival of high-risk patients was 41.4 ± 12.8% in sm-allo-PBHSCT group, while 60.9 ± 9.6% in sm-allo-PBHSCT + BMT group (p = 0.071). It is concluded that the rhG-CSF mobilized sibling matched allo-PBHSCT + BMT is superior to the rhG-CSF mobilized sibling matched allo-PBHSCT in increasing the overall survival of high-risk hematologic malignancies.
Adolescent
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Adult
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Aged
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Bone Marrow Transplantation
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Child
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Child, Preschool
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Female
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Granulocyte Colony-Stimulating Factor
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therapeutic use
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HLA Antigens
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immunology
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Hematologic Diseases
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immunology
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therapy
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Humans
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Male
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Middle Aged
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Peripheral Blood Stem Cell Transplantation
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Retrospective Studies
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Siblings
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Tissue Donors
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Young Adult
6.Nuclear matrix protein 22 and urinary cytology test in the diagnosis of bladder cancer: a meta-analysis.
Hai-yang HU ; Zi-li HU ; Hong-quan WANG ; Chuan LIU ; Sheng-jie YU
Chinese Journal of Surgery 2012;50(12):1126-1130
OBJECTIVESystematic reviews of diagnostic value of the nuclear matrix protein 22 (NMP22) and urine cytology for bladder cancer.
METHODSDevelopment of inclusion criteria, exclusion criteria and search strategy to retrieve relevant literature. Screening the literature according to inclusion criteria and exclusion criteria. Quality evaluation of the screening and data extraction, using MetaDiSc 1.4 software for Meta analysis.
RESULTSIn total, 266 relevant studies were searched, excluded 256 studies, and then 10 studies were included, with 4895 patients involved. The pooled sensitivity and specificity of NMP22 to detect bladder cancer were 0.76 (95%CI: 0.74 - 0.77), 0.80 (95%CI: 0.79 - 0.82), respectively. The pooled sensitivity and specificity of urine cytology were 0.36 (95%CI: 0.34 - 0.38), 0.94 (95%CI: 0.93 - 0.95), respectively. The area under curve (AUC) for NMP22 and urine cytology were 0.8533 and 0.8628, and Q(*) index were 0.7863 and 0.7934, respectively.
CONCLUSIONSFor the diagnosis of bladder cancer, the sensitivity of NMP22 was higher than urine cytology, but the specificity was lower than urine cytology. Overall diagnostic performance of NMP22 was medium, it was no significant difference with urine cytology. It can't replace urine cytology now.
Cytological Techniques ; Humans ; Nuclear Proteins ; analysis ; Sensitivity and Specificity ; Urinalysis ; Urinary Bladder Neoplasms ; diagnosis
7.Functional defect of partial homing receptor on human cord blood hematopoietic stem/progenitor cells.
Xu-Han ZHANG ; Zi-Min SUN ; Hui-Lan LIU ; Xing-Bing WANG ; Liang-Quan GENG
Journal of Experimental Hematology 2010;18(2):445-449
This study was aimed to investigate the function defect of partial homing receptor on cord blood hematopoietic stem cells (CBHSC) and explore efficacy and feasibility of intervention in vitro. The expression and activity of active groups in P, E-selectin ligands on CD34+ cells from cord blood, bone marrow and peripheral blood were detected by flow cytometry; meanwhile the expression of active groups in selectin ligands on CD34+ cells treated by fucosyl transferase in vitro was determined by flow cytometry. The results indicated that the expression levels of CD26 on the surface of stem/progenitor cells (CD34+) from cord blood, bone marrow and peripheral blood were (7.62+/-0.63)%, (6.35+/-0.89)% and (6.18+/-0.91)% (p>0.05) respectively. And the activities of CD26 of the three sources of stem cells were 67.15 U/1000 cells (1 U=1 pmol/min), 26.85 U/1000 cells and 20.95 U/1000 cells respectively, in which the activity of CD26 on surface of CD34+ from cord blood was significantly higher than that from other both sources (p<0.01). The expression levels of P-selectin ligand on the stem/progenitor cells three kinds were (83.46+/-6.33)%, (15.65+/-0.89)% and (80.17+/-6.85)%, and the expression levels of E-selectin ligand on stem/progenitor cells of three kinds were (25.31+/-1.03)%, (26.34+/-0.89)% and (29.79+/-1.78)% respectively. The expression of E-selectin ligand on the surface of cord blood stem/progenitor cell CD34+ increased from (25.31+/-1.03)% to (63.23+/-1.08)% after glycosylation engineering. It is concluded that there is no significant difference of the expression of CD26 between the three sources of stem/progenitor cells, but the activity of CD26 in cord blood was obviously higher than that in bone marrow and peripheral blood. The expression of P-selectin ligand on bone marrow stem/progenitor cell was lower than that on stem cells of cord blood and peripheral blood. Glycosylation engineering can promote and elevate the expression of E-selectin ligand on the surface of CD34+ cells from cord blood.
Antigens, CD34
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metabolism
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Bone Marrow Cells
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cytology
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metabolism
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Cells, Cultured
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Dipeptidyl Peptidase 4
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metabolism
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Fetal Blood
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cytology
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Hematopoietic Stem Cells
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cytology
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metabolism
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Humans
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Receptors, Fibroblast Growth Factor
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metabolism
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Sialoglycoproteins
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metabolism
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Stem Cells
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cytology
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metabolism
8.Protective effects of hydroxyethylpuerarin on cultured bovine cerebral microvascular endothelial cells damaged by hydrogen peroxide.
Hong-mei GUANG ; Xiu-mei ZHANG ; Ying-quan LI ; Xin-bing WEI ; Zi-ying WANG ; Hui-qing LIU
Acta Pharmaceutica Sinica 2005;40(3):220-224
AIMTo observe the damages induced by hydrogen peroxide in cultured bovine cerebral microvascular endothelial cells (BCMEC) and evaluate the protective effects of hydroxyethylpuerarin on hydrogen peroxide-injured BCMEC.
METHODSBCMEC were cultured and transferred into modified Eagle medium (MEM). The viability of cells was detected by MTT assay. Cell injury was determined by lactate dehydrogenase (LDH) activity in the extracellular medium. Flow cytometry was employed to observe the occurrence of apoptosis. Morphologic changes of cells were visualized under phase contrast and electron microscopes.
RESULTSHydrogen peroxide (200 micromol x L(-1) for 4 hours) inhibited the viability of cultured BCMEC and stimulated LDH release. Hydrogen peroxide (100 micromol x L(-1) for 4 hours) induced the occurrence of apoptosis. Hydroxyethylpuerarin was shown to increase the survival rate and decrease the activity of LDH of BCMEC damaged by hydrogen peroxide. Hydroxyethylpuerarin was also found to protect BCMEC against apoptosis induced by hydrogen peroxide.
CONCLUSIONHydrogen peroxide induces BCMEC injury either by apoptosis or through necrosis. Hydroxyethylpuerarin protects BCMEC against hydrogen peroxide-induced injury in a concentration-dependent manner. Its antioxidant effects might be involved as the mechanism protection.
Animals ; Antioxidants ; administration & dosage ; isolation & purification ; pharmacology ; Apoptosis ; drug effects ; Brain ; blood supply ; Cattle ; Cell Survival ; drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; pharmacology ; Endothelial Cells ; drug effects ; metabolism ; Hydrogen Peroxide ; toxicity ; Isoflavones ; administration & dosage ; isolation & purification ; pharmacology ; Microcirculation ; drug effects ; metabolism ; Neuroprotective Agents ; administration & dosage ; isolation & purification ; pharmacology ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Pueraria ; chemistry
9.Relationship between apoptosis induced by 2-butylamino-2-demethoxy-hypocrellin B in human pancreatic cancer cells Capan-1 and photosensitization of mitochondria.
Zi-wen LIU ; Yu-pei ZHAO ; Quan LIAO ; Yuan-de WU ; Yu ZHU
Chinese Journal of Surgery 2006;44(1):36-39
OBJECTIVETo explore the possible mechanism of apoptosis induced by photodynamic therapy (PDT) in human pancreatic cancer cells Capan-1 with 2-butylamino-2-demethoxy-hypocrellin B (BAHB) as photosensitizer.
METHODSThe localization of BAHB in Capan-1 cells was studied, apoptosis was determined by DNA gel electrophoresis after PDT. The mitochondria membrane potential (DYm) and cytochrome C release were observed by laser scan confocal microscopy and Western blotting.
RESULTSThe low concentration photosensitizer was mainly localized in mitochondria and also in lysosomes when the concentration is high. DNA ladder analysis showed characteristic of apoptosis. The mitochondria membrane potential (DYm) showed a loss of 30% around, after 6 hours by PDT under laser scan confocal microscopy, which is caused by a sudden increase in the permeability of mitochondria membrane accompanied with apoptosis. In Western blotting, cytochrome C release was observed from the mitochondria into the cytoplasm during BAHB-induced apoptosis.
CONCLUSIONThe research suggests that BAHB-induced apoptosis is related to photosensitization of mitochondria.
Apoptosis ; drug effects ; Dose-Response Relationship, Drug ; Humans ; Membrane Potentials ; drug effects ; Mitochondria ; drug effects ; physiology ; Pancreatic Neoplasms ; drug therapy ; pathology ; Perylene ; administration & dosage ; analogs & derivatives ; pharmacology ; Photochemotherapy ; Photosensitizing Agents ; administration & dosage ; pharmacology ; Quinones ; administration & dosage ; pharmacology ; Tumor Cells, Cultured
10.Perioperative nutrition support of the patients with pancreatic head cancer.
Quan LIAO ; Yu-pei ZHAO ; Wei-bin WANG ; Meng-hua DAI ; Ya HU ; Zi-wen LIU ; Yu ZHU
Acta Academiae Medicinae Sinicae 2005;27(5):579-582
OBJECTIVETo explore the effect of perioperative nutrition support on nutritional condition and complications of the patients with postoperative pancreatic head cancer.
METHODSThirty four patients received perioperative nutrition support, including enteral nutrition and parenteral nutrition (treatment group). Forty eight patients received routine postoperative parenteral nutrition (control group). According to the operative method, these two groups were further divided into two sub-groups: (1) pancreaticoduodenectomy (PD) subgroup, including 13 cases from treatment group, and 24 cases from control group; (2) palliative operation subgroup, including 21 cases from treatment group, and 24 cases from control group. Body weight, total protein (TP), serum albumin (ALB), and the complications after operation were compared.
RESULTSThe concentrations of ALB and TP in the treatment group were significantly higher than those in the control group (P< 0.05). Body weight and TP of the patients received PD in the treatment group were significantly better than those of the control group (P < 0.05).
CONCLUSIONPerioperative nutrition support can improve postoperative nutritional condition and reduce the postoperative complications in patients with pancreatic head cancer.
Adult ; Aged ; Combined Modality Therapy ; Enteral Nutrition ; Female ; Humans ; Male ; Middle Aged ; Nutritional Support ; methods ; Pancreatic Neoplasms ; surgery ; therapy ; Pancreaticoduodenectomy ; Parenteral Nutrition ; Postoperative Complications ; prevention & control