Objective To study the space distribution probability model of death cases of hemorrhagic fever with renal syndrome,so as to provide basic information for hemorrhagic fever with renal syndrome prevention and control policy making.Methods Poisson distribution and negative binomial distribution were used to fit the hemorrhagic fever with renal syndrome deaths spatial distribution in Kaijiang county,Dazhou city,Sichuan province between 1979-2010.Results The actual distribution of death cases of hemorrhagic fever with renal syndrome in Kaijiang county was not Poisson distribution (x2 =40.64,P ＜ 0.01),but subjected to the negative binomial distribution(x2 =1.86,P ＞ 0.50).Conclusions The space distribution of death cases of HFRS in Kaijiang county is not random,but has strict regional aggregation,which may be related to the local medical conditions,patient' s lack of knowledge about health care and other factors.

0.05). However, there were quite improvements of survival rate and survival quality for cervical adenocacinoma. Their 5-year survival rates were 91% and 33% (P

Objective To explore the application of computer-assisted telephone interviewing in the follow-up study of endemic diseases,and to provide a reference and basis for wide range application of this method.Methods In this investigation,146 patients with Keshan disease from 2 provinces were recruited in.The study mainly included two questionnaire surveys:disease follow-up questionnaire survey and cardiac insufficiency quality of life scale survey.Survey methods included computer-assisted telephone survey and face-to-face questionnaire survey.In the 18-month of follow-up study,one computer-assisted telephone survey was done in the 3ed,6th,12th,15th month,and one face-to-face questionnaire survey in 9th and 18th month.Time-consuming and quality of the two survey methods were eompaired.The computer-assisted telephone survey database was designed using Epi Info 3.32software.Results The computer-assisted telephone survey took an average of (13.10 t 1.80)min,and face-to-facequestionnaire survey took an average of (22.04 ± 2.28)min,the difference was statistically significant (t =- 36.214,P ＜ 0.05).The error rate of computer-assisted telephone survey was 6.80％ (37/544),and that of face-to-face questionnaire survey was 11.07％(28/253),the difference was statistically significant(x2 =4.195,P ＜ 0.05).The quality of computer-assisted telephone survey was better than that of face-to-face questionnaire survey.Conclusions Through the computer-assisted telephone interviewing,the work efficiency of investigators is improved during the follow-up study of Keshan disease.The validation function improves the accuracy rate during the process of data entry.Because of the application of telephone contact,a lot of research funding is saved.

Propionic acid, a major inhibitor to yeast cells, was accumulated during continuous ethanol fermentation from corn meal hydrolysate by the flocculating yeast under stillage backset conditions. Based on its inhibition mechanism in yeast cells, strategies were developed for alleviating this effect. Firstly, high temperature processes such as medium sterilization generated more propionic acid, which should be avoided. Propionic acid was reduced significantly during ethanol fermentation without medium sterilization, and concentrations of biomass and ethanol increased by 59.3% and 7.4%, respectively. Secondly, the running time of stillage backset should be controlled so that propionic acid accumulated would be lower than its half inhibition concentration IC50 (40 mmol/L). Finally, because low pH augmented propionic acid inhibition in yeast cells, a higher pH of 5.5 was validated to be suitable for ethanol fermentation under the stillage backset condition.
Biomass ; Ethanol ; metabolism ; Fermentation ; Flocculation ; Propionates ; chemistry ; Yeasts ; metabolism

Objective To observe the relationship between rs42524 polymorphisms of collagen Ⅰ COL1A2 gene and atherosclerotic cerebral infarction, stability of carotid arteriosclerotic plaque.Methods Carotid ultrasound detection by Color Doppler ultrasonography was used to detect 289 patients with acute atherosclerotic cerebral infarction.These patients were divided into stable plaque and vulnerable plaque subgroups according to the carotid atherosclerotic plaque stability.Then 107 healthy individuals who had no carotid plaque were collected as the control group.Rs42524 polymorphism of COL1A2 gene was detected by PCR-restriction fragment length polymorphism.The results were analyzed.Results There were no significant differences of the frequencies of genotypes and alleles of rs42524 of COL1A2 gene between the cerebral infarction group and the control group (all P>0.05).According to the results of carotid ultrasound detection, the cerebral infarction group patients were divided into stable plaque subgroup including 108 cases, and vulnerable plaque subgroup including 181 cases.Rs42524 of COL1A2 gene showed no significant differences in the frequencies of alleles and genotypes between stable plaque subgroup and vulnerable plaque subgroup ( all P>0.05) .Conclusion Rs42524 polymorphism of COL1A2 gene is meaningless to the onset risk of atherosclerotic cerebral infarction and the stability of carotid atherosclerotic plaque.

Objective To explore the effect of atorvastatin on high-sensitivity C-reactive protein (hs-CRP) and interleukin-6 (IL-6) in unstable angina pectoris (UAP) patients after intervention.Methods Eighty cases with UAP who underwent percutaneous coronary intervention (PCI) were divided into group A and group B by random digits table method with 40 cases each after PCI.The patients in group A were given regular heart treatment and atorvastatin 20 mg treatment.The patients in group B were given regular heart treatment and atorvastatin 40 mg treatment.The serum hs-CRP and IL-6 was determined before treatment and 24 h,3 weeks after treatment and compared between two groups.Results There was no significant difference in the serum hs-CRP and IL-6 before treatment between group A and group B [(5.6 ± 1.2) mg/L vs.(5.6 ± 1.1) mg/L and (211.9 ± 21.2) ng/L vs.(209.6 ± 19.9) ng/L,P ＞ 0.05].The serum hs-CRP and IL-6 24 h after treatment in group A and group B were increased compared with that before treatment,and there was significant difference(P ＜ 0.05),but there was no significant difference between group A and group B[(8.1 ± 1.1) mg/L vs.(8.5 ± 1.2) mg/L and (311.1 ± 20.9) ng/L vs.(313.3 ± 18.5) ng/L,P＞ 0.05].The serum hs-CRP and IL-6 3 weeks after treatment in group A and group B were decreased compared with that before treatment,there were significant difference (P ＜ 0.05),and there were significant difference between group A and group B [(3.1 ± 1.1) mg/L vs.(1.9 ±0.8) mg/L and (163.3± 18.5) ng/L vs.(123.3± 19.5)ng/L,P ＜ 0.05].No obvious adverse reaction was observed in two groups after treatment and liver function was not seen obvious anomaly.Conclusions Atorvastatin treatment in UAP patients after PCI can significantly reduce the serum hs-CRP and IL-6,and the 40 mg treatment is better than the 20 mg treatment.It is worth of clinical application.

Objective To investigate the expression and significance of KAI1/CD82 and E-Cadherin in human squamous cervical carcinoma.Methods The expressions of KAI1/CD82 and E-Cadherin were examined respectively by immunohistochemical S-P method in 10 cases of normal cervical tissue,15 cases of atypical hyperplasia of cervical epithelia and 64 cases of squamous cervical carcinoma.We analyzed statistically the correlation between the immunohistochemical results and the clinicopathological features.Results From normal cervical tissue,Cervical intraepithelial neoplasis(CIN) to cervical cancer,the positive expression rates of KAI1/CD82 and E-Cadherin were gradually decreased.Through statistic test,the positive expression rates of both invasive squamous carcinoma of the cervix were significantly lower in normal cervical tissue and CIN(P0.05).Of all the samples,direct correlation was showed in the expressions of KAI1/CD82 and E-Cadherin.Furthermore,the low-expression of both revealed significant correlation with lymphonode metastasis in human squamous cervical carcinoma.Conclusion KAI1/CD82 and(E-Cadherin) interactions may depress lymphonode metastasis in cervical carcinoma.Logistic analysis reveals that(E-Cadherin) gene,specific and interactions of several genes may be more significant during tumor metastasis.

Objective: To study the factors which influence the dose distribution of brachytherapy in cervical cancer. Methods: Ninety-five patients with cervical cancer II - III b received fundamental radiation therapy including brachytherapy in our department from Aug. 2004 to Nov. 2005. The deviation of isodose curve of brachytherapy was based on A-B reference system, and the deviation of dose was defined by measuring in a practical standard body model. Results: The factors influencing isodose offset significantly were parametrial infiltrating degree, and anatomy factor of cervical cancer and operating skill. The degree of isodose offset could not be lowered with the increased frequency of brachytherapy. Conclusion: Making simulation in cervical brachytherapy is necessary not only for the identification of the deviation of isodose curve but also for adjusting the dose distribution and revising the plan of radiotherapy.

Objective: To study the factors which influence the dose distribution of brachytherapy in cervical cancer. Methods: Ninety-five patients with cervical cancer II - III b received fundamental radiation therapy including brachytherapy in our department from Aug. 2004 to Nov. 2005. The deviation of isodose curve of brachytherapy was based on A-B reference system, and the deviation of dose was defined by measuring in a practical standard body model. Results: The factors influencing isodose offset significantly were parametrial infiltrating degree, and anatomy factor of cervical cancer and operating skill. The degree of isodose offset could not be lowered with the increased frequency of brachytherapy. Conclusion: Making simulation in cervical brachytherapy is necessary not only for the identification of the deviation of isodose curve but also for adjusting the dose distribution and revising the plan of radiotherapy.

Objective To study the clinic features and prognosis of juvenile rheumatoid arthritis (JRA)-associated iridocyclitis.Methods Ten cases of JRA -associated iridocyclitis choosed from 107 cases with JRA were reviewed and analysed.Results Polyarticular( n =9) and pauciarticular( n =1) were observed among JRA -associated iridocyclitis.Four were acute cases and all were recovered.Secondary glaucoma ( n =1) and ablepsia( n =1) were find in the chronic cases of 6,respectively.Conclusions Iridocyclitis is a common complication of JRA. Most of them are polyarticular group. The acute cases have better results compared to the chronic cases which often have more complications such as glaucoma,cataract, et al .