Child ; Graft Survival ; HLA Antigens ; genetics ; immunology ; Hematopoietic Stem Cell Transplantation ; Humans ; Polymerase Chain Reaction ; Tissue Donors ; Transplantation Chimera ; genetics ; Transplantation Conditioning ; methods ; Transplantation, Homologous ; immunology

Lysins are efficient bacteria cell wall digesting enzymes encoded by DNA bacteriophage. Gram-positive bacteriophage lysins feature similar domain structure, high lytic efficiency, synergic antibacterial effect with antibiotics, rare neutralization by antibodies, less chance of developing drug-resistant strains, et al. The past decade has seen a considerable amount of research worldwidely focused on lysin, and lysins have been used successfully in a variety of animal models to control pathogenic antibiotic resistant bacteria found on mucosal surfaces and infected tissues. The great potential of lysins as an anti-infective agent prompted this review.

The optimum medium for Lactobacillus M7 was systematically studied with SAS system. Firstly, the prime factors affecting lactic acid yield were selected by means of Plackett-Burman design; secondly, the pnme facias were optimized by response rurface analysis. Under the optimum level determined, the yield is increased by 15%.

Objective To explore the effect of Xinwei Granules on cell proliferation in rats with precancerous lesions of gastric cancer based on CyclinD1-CDK4/6-P16 pathway.Methods 180 Wistar rats were randomly divided into 6 groups,30 rats in each group:blank control group(blank),model group,Weifuchun control group,Xinwei Granule(low,medium and high dose)group.The PLGC rat model was made by MNNG method.Xinwei granule low,medium and high dose group were 0.72 g·kg-1,1.44 g·kg-1,2.88 g·kg-1;weifuchun group was given 0.52 g·kg-1,once a day by intragastric administration,and the blank group and the model group were given the same amount of normal saline by intragastric administration.The experimental period was 16 weeks.After the end of the experiment,the general condition of the rats was observed,and the mRNA and protein expressions of CyclinD1,CDK4/6 and P16 in gastric mucosa were detected by RT-PCR and Western blotting.Results The activity and diet of rats in each group of Xinwei Granules and Weifuchun group were improved to varying degrees compared with the model group.Compared with the blank control group,the mRNA and protein expression levels of CyclinD1,CDK4 and CDK6 in the model group were significantly increased.The mRNA and protein expression levels of P1 6 were significantly decreased(P＜0.05).The expression levels of CyclinD1,CDK4,CDK6 mRNA and protein in Xinwei granule(low,medium and high dose)group and Weifuchun group were lower than those in model group.The mRNA and protein expression levels of P16 were significantly increased(P＜0.05),and the effect of Xinwei granule medium dose group was the most significant.Conclusion Xinwei granules participate in the regulation of cell cycle through the CyclinD1-CDK4/6-P16 pathway,thereby inhibiting the proliferation of PLGC cells.

Central composite design (CCD) is one of the most commonly used design methods in response surface optimization and has been widely applied in the field of pharmaceutics to optimize preparations. On the 20th anniversary of the introduction of CCD into China, the paper reviews its application in domestic pharmaceutical researches. Based on the brief introduction of basic principle and operation steps of CCD, the mistakes emerging in the application of CCD are summarized, including conceptual confusion with Box-Behnken design and face-centered CCD as well as wrong designs. Besides, the issues concerning the selection of factors and responses are discussed. The article is helpful for researchers to comprehensively understand the CCD and facilitates the rational application of this method.

Objective To study the effects and mechanisms of BuShenYangGuTang on proliferation and apoptosis of multiple myeloma cell line KM3. Methods The inhibitory effect of BuShenYangGuTang on cell proliferation was assessed by CCK-8. BuShenYangGuTang induced KM3 cell cycle arrest was analyzed by flow cytometry after propidium iodide staining. Flow cytometry was also used to analyze the cell apoptosis after Annexin V-FITC staining. The expressions of Bcl-2, Bax and NF-κB were determined by RT-qPCR and Western blot assay. Results BuShenYangGuTang inhibited the KM3 cell proliferation in a dose-dependent manner. The expression levels of Bcl-2 and NF-κB were decreased, the expression level of Bax was increased, and the cell cycle was arrested in G0 / G1 phase after treatment with BuShenYangGuTang. Conclusion BuShenYangGuTang could inhibit the proliferation, arrest cell cycle and induce the apoptosis in KM3 cells, which may be related to the abnormal expressions of Bcl-2, Bax and NF-κB.

Animals ; History, 20th Century ; Humans ; Influenza A Virus, H1N1 Subtype ; genetics ; immunology ; isolation & purification ; Influenza, Human ; epidemiology ; history ; immunology ; virology ; Russia ; epidemiology

Animals ; Global Health ; History, 20th Century ; Humans ; Influenza A Virus, H1N1 Subtype ; genetics ; isolation & purification ; metabolism ; pathogenicity ; Influenza, Human ; epidemiology ; history ; virology ; Spain ; epidemiology ; Viral Proteins ; genetics ; metabolism

Action Potentials ; drug effects ; Calcium Channel Blockers ; pharmacology ; Cinnarizine ; analogs & derivatives ; pharmacology ; Heart Atria ; Humans ; Isoproterenol ; antagonists & inhibitors ; Microelectrodes ; Purkinje Fibers ; drug effects ; physiology