To clone and sequence the human tissue kallikrein gene of Chinese, and to construct eukaryotic expression recombinant of KK, total RNA was extracted from human pancreas and human tissue kallikrein gene cDNA was amplified by PCR after reverse-transcription by using Oligo(dT) primer. The original kallikrein cDNA was recovered and filled with Klenow enzyme and inserted into KS plasmid. After restriction endonuclease digestion, KK cDNA was sequenced by ABI377 analyzer. Then the KK gene was amplified from pBluescript KSKK and inserted into pcDNA3. A sequence comparison showed that the cloned kallikrein gene was only one nucleotide different from that reported in the Genbank. The coding amino acid was Asp in the Genbank gene, while the coding amino acid of Chinese kallikrein gene was Asn. The KK cDNA fragment was inserted into the eukaryotic expression vector pcDNA3. The cloned kallikrein gene and the pcDNA3KK can be used for further study in gene therapy.. .

Objective To explore the effects of course diary in preventing lumen catheter infection in patients who received hemodialysis (HD).Methods Totally 105 patients who received HD in Civil Aviation General Hospital between January 2013 and December 2016 were selected by convenience sampling and divided into an observation group (53 patients) and a control group (52 patients) according to the random number table. Patients in the control group received conventional nursing, while patients in the observation group, on this basis, were supervised by nurses to implement the course diary including content related to infection prevention, and the nurses undertook to keep records. 12 weeks later, the blood flow volume, urea clearance index (KT/V), incidence of insufficient blood flow and infection rate were compared between the patients in the two groups.Results The patients in the control group showed a higher blood flow volume than the patients in the observation group (P<0.05), and there was no statistically significant difference in the urea clearance index (P>0.05) before the intervention, but the patients in the observation group showed a higher blood flow volume and urea clearance index than the patients in the control group (P<0.05) after the intervention. There was no statistically significant difference in the incidence of insufficient blood flow (P>0.05) before the intervention, while the patients in the observation showed a lower incidence of insufficient blood flow than the patients in the control group (P<0.05) after the intervention. And the patients in the observation group showed a lower infection rate than the patients in the control group (P<0.05) after the intervention.Conclusions Course diary can help to improve the blood flow volume and urea clearance index, reduce the incidence of pathogens, and lower the infection rate in patients receiving HD with lumen catheter.

To clone and sequence the human tissue kallikrein gene of Chinese, and to construct eukaryotic expression recombinant of KK, total RNA was extracted from human pancreas and human tissue kallikrein gene cDNA was amplified by PCR after reverse-transcription by using Oligo(dT)primer. The original kallikrein cDNA was recovered and filled with Klenow enzyme and inserted into KS plasmid. After restriction endonuclease digestion, KK cDNA was sequenced by ABI 377 analyzer.Then the KKgene was amplified from pBluescript KSKK and inserted into pcDNA3. A sequence comparison showed that the cloned kallikrein gene was only one nucleotide different from that reported in the Genbank. The coding amino acid was Asp in the Genbank gene, while the coding amino acid of Chinese kallikrein gene was Asn. The KK cDNA fragment was inserted into the eukaryotic expression vector pcDNA3. The cloned kallikrein gene and the pcDNA3KK can be used for further study in gene therapy...