Objective To study the effect of matrine on the expression of a proliferation-inducing ligand (APRIL) in colorectal cancer cell line (SW480 cell). Methods MTT assay was used to evaluate the inhibitory effect of matrine on SW480 cells. The protein and mRNA levels of APRIL in SW480 cells were determined by immunohistochemistry and real-time fluorescence quantitative PCR (RFQ-PCR). SW480 cells were treated with 0.5,1.0,2.0 mg/ml of matrine for 24 h, 48 h and 72 h. FU and blank were served as drug control and blank control groups, respectively. Results Matrine had obviously inhibitory effect on proliferation of SW480 cells in a time- and dose-dependant manner. The expression of APRIL was strong in SW480 cells. When treated with 50,100,200 ug/ml of FU, the APRIL mRNA levels in SW480 cells raised gradually and reached the highest levels at 72 h after treatment, which were significantly higher than those in blank control group (all P value<0.001). When treated with 0. 5,1.0, 2.0 mg/ml of matrine, the APRIL mRNA levels in SW480 cells increased at 24 h after treatment, which were significantly higher than those in blank control group (all P value<0. 001), and then decreased gradually and almost equal to level of blank control group at 72 h. Conclusion In treatment with FU, the survival cells.may have stronger ability of proliferation due to higher expression of APRIL in SW480 cells. Anti-APRIL therapy might be an important assistant treatment to counter the impact of APRIL. Matrine will not cause persistent increase of APRIL mRNA levels in SW480 cells, so it might be a helpful drug in anti-tumor theraphy.

Objective To investigate the value of emergent endoscopy in the acute nonvariceal gastrointestinal bleeding.Methods Retrospective analysis was done in patients with melena or haematemesis in the ward from Jan 1st 2003 to Dec 31st 2007.For emergent group,the patients took endoscopy in 48 h after bleeding.For selective group,the patients took endoscopy after 48 h.Medical outcomes and resource utilization were compared between two groups.Results The average age of 332 patients(246 males and 86 females)was(45.9?18.8)years old.In the median and low risk patients,the length and the cost of hospitalization,blood transfution and the use of PPI were lower in the emergent group than in the selective group.In the high risk patients,the above variables had no statistical difference.Conclusion For the patients whose Rockall Score were less than 5,emergent endoscopy could decrease the length and the costs of hospitalization,the transfusion volumes and the use of PPI.However,for the patients whose Rockall Score were more than 5,the advantage of emergent endoscopy need further discussion.

Objectives:To investigate the risk factors of cage retropulsion(CR)after posterior lumbar inter-body fusion(PLIF).Methods:Retrospective analysis was made on 17 patients(13 males and 4 females,with an average age of 63.7±9.9 years)who underwent revision surgery due to CR after PLIF in our hospital from September 2017 to September 2021.And the patients were included into the CR group.Another 34 patients without CR were matched in a ratio of 2∶1 according to the same fusion and fixation segments,time of initial surgery(±1 year),gender,and age(±2 years),including 26 males and 8 females,with an average age of 65.2±10.2 years.They were included in the control group.In the CR group,the average number of fixed segments was 1.8±0.8,and the average number of fused segments was 1.5±0.6;The time of CR was 7(0.75-132)months after the first operation;15 patients had single segmental CR and 2 patients had two segmental CR.The lumbar lordosis(LL),pelvic tilt(PT),sacral slope(SS),and pelvic incidence(PI)were measured on full-spine X-ray before operation of the two groups of patients,the disc height(DH)of the diseased segment was mea-sured on lateral lumbar X-ray,and the intervertebral range of motion(ROM)was measured on flexion-exten-sion X-ray.The CT value of lumbar vertebrae was measured in CT examination before operation;The shape of disc was defined through MRI examination.The cage position was measured on X-ray immediately after operation(The ratio of the distance between the marker line of the posterior margin of the cage and the pos-terior upper margin of the lower vertebral body to the length of the upper endplate of the lower vertebra).The paired sample t-test was used for single factor analysis to analyze the data of the two groups,after which,the parameters with statistical significance were analyzed with logistic regression to determine the inde-pendent risk factors of CR.Results:The mean vertebral CT value in the CR group was lower than that in the control group(124.8±39.7 vs 147.7±38.2,P=0.011);The cage position in the CR group was more posterior than that in the control group(0.15±0.09 vs 0.31±0.07,P＜0.001).There was no significant difference between the two groups in LL(40.8°±12.9° vs 42.4°±7.5°,P=0.717),PT(19.6°±7.1° vs 17.1°±6.7°,P=0.356),SS(27.7°±6.5° vs 31.0°±4.3°,P=0.144),PI(44.3°±13.8° vs 44.7°±13.9°,P=0.926),DH(10.1±2.4mm vs 8.8±1.4mm,P=0.066),ROM(4.3°±2.8° vs 4.4°±2.2°,P=0.950),and Pear-shaped intervertebral discs(33.3％vs 21.4％,P=0.40).Logistic regression analysis showed that low vertebral CT value(osteoporosis)(OR=0.975,P=0.043)and cage po-sition(OR=28.393,P=0.003)were the independent risk factors.Conclusions:Osteoporosis and posterior placement of cage are the risk factors for CR after PLIF.

PURPOSE: Recent evidence suggests that B cells can both promote and inhibit the development and progression of allergic disease. However, the characteristics of B cell subsets in patients with allergic rhinitis (AR) have not been well documented. This study aimed to analyze the characteristics of B cell subsets in the peripheral blood of AR patients. METHODS: Forty-seven AR patients and 54 healthy controls were enrolled in this study, and the B cell subsets in peripheral blood of all subjects were analyzed by flow cytometry. Moreover, the serum total immunoglobulin E (IgE) and IgE concentrations secreted into the cultured peripheral blood mononuclear cells (PBMCs) were measured by using enzyme-linked immunosorbent assay. RESULTS: We found the peripheral blood of AR patients contained higher percentages of memory B cells, plasma cells, and CD19+CD24hiCD27+ regulatory B cells (Bregs) than those of age-matched healthy controls (P < 0.05), while the percentages of naïve B cells and CD19+CD24hiCD38hi Bregs were significantly lower in AR patients than in healthy individuals (P < 0.05). In addition, the serum total IgE and IgE concentrations secreted into the cultured PBMCs were elevated in AR patients than in the healthy controls (P < 0.05). CONCLUSIONS: Our findings indicate that AR patients were characterized by increase in terminally differentiated memory B cells or plasma cells and decreases in CD19+CD24hiCD38hi Breg cells in the peripheral blood.
B-Lymphocyte Subsets* ; B-Lymphocytes ; B-Lymphocytes, Regulatory ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Humans ; Immunoglobulin E ; Immunoglobulins ; Memory ; Plasma Cells ; Rhinitis, Allergic*

Radiomics-based early prediction and treatment efficacy evaluation is critical for personalized treatment strategies in patients with colorectal cancer liver metastases(CCLM).Owing to the high artificial intelligence(AI)participation,repeatability,and reliable perform-ance,deep learning(DL)based on convolutional neural networks enhances the predictive efficacy of the models,enabling its potential clinic-al application more promising.Subsequent to the gradual construction of a multimodal fusion model and multicenter large sample database,radiomics and DL will become increasingly essential in the management of CCLM.This review focuses on the main steps of radiomics and DL,and summarizes the value of its application in early state prediction and treatment efficacy evaluation of different treatment modalities in CCLM,we also look forward to the potential of its in-depth application in the clinical management of CCLM.