AIMTo improve specificity and accuracy of endogenous ouabain measurement assay.

METHODSAnti-ouabain polyclonal antibody egg yolk (IgY) and anti-ouabain rabbit antibody (IgG) were prepared respectively. In the presence of two kinds of antibody, then the specificity and accuracy of enzyme-linked immunosorbent assay (ELISA) were compared.

RESULTSThe ELISA, in the presence of IgY, provided a sensitivity of the average intraassay coefficient of variation(CV) was 2.03%, and the inter-assay CV was 2.34% respectively. In contrast, IgG were 2.83% and 3.29%. No significant interferences were observed with hydrocortisone and dexamethasone. There was 3.45% vs. 5.95%, 3.20% vs. 5.20% of crossreaction with cedilanid and digoxin.

CONCLUSIONThe specificity and accuracy of ELISA, in which IgY was used, were more better than IgG.

Animals ; Antibody Specificity ; Chickens ; immunology ; Cross Reactions ; Egg Yolk ; immunology ; Enzyme-Linked Immunosorbent Assay ; methods ; Immunoglobulin G ; immunology ; Immunoglobulins ; immunology ; Male ; Ouabain ; analysis ; Rabbits