1.CT perfusion study of acute local cerebral infarction in rhesus monkeys.
Zhuo-kai HE ; Yi-kai XU ; Wei-jia QIU ; Yan-xian DENG ; Zhi-peng ZHOU ; Zhi-hong HUANG ; Fei-ling FENG ; Ying WEI ; Rui-jing GAN ; Jun-xiong YU
Journal of Southern Medical University 2007;27(5):720-724
OBJECTIVETo explore the value of CT perfusion in early diagnosis and management of superacute local cerebral infarction in rhesus monkeys.
METHODAcute local cerebral infarction was induced in the rhesus monkeys during digital subtraction angiography (DSA) by introduction of pale thrombus prepared from autologous blood into the M1 branch of the middle cerebral artery (MCA). Plain CT scan and CT perfusion scanning were performed at different time points before and after DSA operation, and the results were analyzed in conjunction with the pathologic changes.
RESULTSIschemic lesions were displayed on CT perfusion images, which showed local hypoperfusion, reduced cerebral blood flow and volume, and mean transit time delay in the compromised area. Local hypointense infarct area was identified in plain CT scan 24 h after the DSA operation, and the results were in good agreement with pathological examination during autopsy.
CONCLUSIONCT perfusion imaging of the brain can accurately capture the cerebral perfusion deficits in acute ischemic stroke before morphologic changes take place, and therefore provides good means for thrombolytic treatment evaluation of stroke.
Acute Disease ; Animals ; Brain ; diagnostic imaging ; Brain Ischemia ; diagnosis ; Cerebral Infarction ; diagnosis ; Contrast Media ; administration & dosage ; Early Diagnosis ; Macaca mulatta ; Perfusion ; Sensitivity and Specificity ; Tomography, X-Ray Computed ; methods
2.Cloning and expression of the succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum Chinese strain in E. coli.
Jun-long YU ; Shi-ping WANG ; Zhuo HE ; Gan DAI ; Xiao-xin JIANG ; Shao-hua ZENG ; Xiao-qing XIAO ; Song-hua ZHOU ; Wen-kai LI ; Shao-rui XU ; Zhi-yue LÜ ; Xian-chu PENG
Journal of Central South University(Medical Sciences) 2006;31(4):458-463
OBJECTIVE:
To clone the full-length gene encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) Chinese strain and express it in Escherichia coli.
METHODS:
According to the published incomplete EST (BU804141) of SjSDISP and the sequence of multiclone sites of lambda gt11 vector, 2 pairs of primers were designed and synthesized. Then the 3' and 5'ends of the EST of the SjSDISP from adult Schistosoma japonicum cDNA library were amplified by anchored PCR. After sequencing, a full-length cDNA sequence of the SjSDISP was obtained, and then it was cloned into prokaryotic expression vector pGEX-4T-1. Identified by agarosed gel electrophoresis, endonucleases digestion and PCR, the resultant recombinant plasmid was used for the expression under the temperature-dependent condition and Western blot analysis.
RESULTS:
A 1,071 bp sequence was obtained. Sequence analysis showed that the fragment contained a complete open reading frame (ORF), encoding 278 amino acid residues. This target fragment was cloned into the prokaryotic expression vector pGEX-4T-1, and expressed in Escherichia coli. SDS-PAGE revealed that the molecular weight of the expressed fusion recombinant product was 56 kD. Western blot showed that the recombinant protein was recognized by polyclonal rabbit antiserum immunized with Schistosoma japonicum adult worm antigen.
CONCLUSION
Cloning of the full-length gene encoding SjSDISP and its bacterial expression were successfully done.
Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Escherichia coli
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metabolism
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Helminth Proteins
;
biosynthesis
;
genetics
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Iron-Sulfur Proteins
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biosynthesis
;
genetics
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Molecular Sequence Data
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Recombinant Proteins
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biosynthesis
;
genetics
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Schistosoma japonicum
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genetics
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metabolism
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Sequence Homology
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Succinate Dehydrogenase
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biosynthesis
;
genetics
3.Study on Sub-minimal Inhibitory Concentration of Tanreqing Injection in Inhibiting Hemolytic Activity of Staphylococcus aureus α-hemolysin
Qian TONG ; Yi WANG ; Jin-he ZHOU ; Kai-yu CUI ; Zhuo MA ; Wei-feng YANG
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(9):86-93
Objective:To study whether Tanreqing injection (TRQ) can alleviate the body injury in the process of infection by inhibiting the production and release of
4.Synthesis and biological evaluation of novel tanshinone IIA derivatives for treating pain.
Qi-Nan LI ; Zhi-Peng HUANG ; Qin-Lan GU ; Zhuo-Er ZHI ; Yu-Han YANG ; Long HE ; Kai-Li CHEN ; Jin-Xin WANG
Chinese Journal of Natural Medicines (English Ed.) 2018;16(2):113-124
Due to ineffectiveness and side effects of existing analgesics, chronic pain has become one of the most complex and difficult problems in the clinic. Monoacylglycerol lipase (MAGL) is an essential hydrolase in the endocannabinoid system and has been identified as a potential target for the treatment of pain. In the present study, we designed and synthesized twelve tanshinone IIA analogs and screened their activity against MAGL. Selected compounds were tested for analgesic activity in vivo, with the acetic acid writhing test model. Among the test compounds, compound III-3 (IC 120 nmol·L) showed significant activity against MAGL and ameliorated the clinical progression in the mouse pain model. Additionally, compound III-3, substitution with N-methyl-2-morpholinoacetamide, demonstrated improved solubility relative to tanshinone IIA.
Abietanes
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administration & dosage
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chemical synthesis
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chemistry
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Analgesics
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administration & dosage
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chemical synthesis
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chemistry
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Animals
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Chronic Pain
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drug therapy
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enzymology
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Drug Evaluation, Preclinical
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Enzyme Inhibitors
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administration & dosage
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chemical synthesis
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chemistry
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Female
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Humans
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Male
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Mice
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Mice, Inbred ICR
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Monoacylglycerol Lipases
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antagonists & inhibitors
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metabolism
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Structure-Activity Relationship
5.Clinical analysis of 10 patients of acute promyelocytic leukemia with a variant RARα translocation.
Rui Hua MI ; Lin CHEN ; Jia LIU ; Tao LIU ; Kai WANG ; Li Hua DONG ; Xu LI ; Yu Zhuo HE ; Zheng Biao LIU ; Xue Jun GUO ; Shu Li GUO ; Hong Mian ZHAO ; Jia Hong TANG ; Xiao Miao MA ; Yu Fu LI ; Xu Dong WEI
Chinese Journal of Hematology 2020;41(3):257-260