The aim of this study was to explore the protective effect of compound tianpupian (TPP) against (2)O(2)-induced the apoptosis of murine splenic lymphocytes and its mechanism. The cell apoptosis rate was detected by MTT method; the cell apoptosis and mitochondrial membrance potential were detected by flow cytometry (FCM) with Annexi-V/PI double staining and JC-1 staining method, respectively; and caspase 3 relative activity was determined by colorimetry. The results indicated that after treating with (2)O(2), the absorbance value of cultured lymphocytes and the red/green ratio of JC-1 were reduced, and the apoptotic rate and caspase 3 activity were increased, coculture of (2)O(2)-treated cells with compound TPP increased the cell absorbance ratio and red/green rate of JC-1, while reduced the apoptosis rate and caspase 3 activity. It is concluded that compound TPP alleviates intracellular oxidative damages and dose-dependently inhibited apoptosis of murine splenic lymphocytes through reducing mitochondrial membrane potential and inhibiting caspase 3 activity. This suggests that compound TPP is a potential anti-apoptotic agent.
Animals ; Apoptosis ; drug effects ; Caspase 3 ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Grape Seed Extract ; pharmacology ; Hydrogen Peroxide ; adverse effects ; Lymphocyte Count ; Lymphocytes ; cytology ; drug effects ; Mice ; Mice, Inbred ICR ; Proanthocyanidins ; pharmacology ; Rhodiola

OBJECTIVETo investigate the best combination of monoclonal antibodies for the diagnosis of hepatobiliary intraepithelial neoplasia and/or cancer.

METHODSCK7, CK20, Villin, CEA, P53 and Ki-67 antigens were detected in the tissues of high-level hepatobiliary intraepithelial neoplasia and cancer by immunohistochemistry and the results were analyzed statistically.

RESULTSVillin was 100% positive in hepatobiliary intraepithelial neoplasia and/or cancer, while 100% negative in the adjacent normal bile duct epithelium. The expression rate of CEA was significantly lower in high-level hepatobiliary intraepithelial neoplasia tissues than in the cancer tissues (P<0.05). Ki-67 indexes were significantly lower in most of the high-level hepatobiliary intraepithelial neoplasia than in the cancer tissue (P<0.01). P53 indexes were also lower in high-level hepatobiliary intraepithelial neoplasia (P<0.01).

CONCLUSIONDetection of multiple antigens (CEA, Villin, Ki-67 and P53) provides specific clues to the diagnosis of high-grade hepatobiliary intraepithelial neoplasia and/or cancer.

Adult ; Aged ; Antibodies, Monoclonal ; Antigens, Neoplasm ; analysis ; Bile Duct Neoplasms ; diagnosis ; pathology ; Bile Ducts, Intrahepatic ; pathology ; Carcinoma in Situ ; diagnosis ; pathology ; Epithelium ; pathology ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged

Objective To investigate the protective effect of prostaglandin E1 on renal injury in rats with obstructive jaundice.Methods The Wistar rats were randomly divided into three groups (n =15):sham operation group,model group and experimental group.The rat model of obstructive jaundice was established by double ligation of common bile duct.The experimental group rats were injected intraperitoneally with prostaglandin E1 0.1 μg · kg-1 on the seventh postoperative day and treated for 14 d.The other two groups were given the same dose of normal saline.The expression of nuclear factor-κB (NF-κB),B lymphocyto tumor-2 (Bcl-2) and intercellular adhesion molecule-1 (ICAM-1) protein were detected by immunohistochemistry.The tubular epithelial apoptosis index was detected by TdT-mediated dUTP nick-end labeling method.Results The expression of NF-κB protein in sham operation group,model group and experimental group were 1.16 ±0.24,9.28 ± 0.48 and 6.52 ± 0.72;the expression of Bcl-2 protein in the three groups were 0.86 ± 0.54,11.28 ± 0.27 and 8.36 ± 0.48;the expression of ICAM-1 protein in the three groups were 1.04 ± 0.52,18.80 ± 3.26 and 11.18 ± 4.62;the renal tubular epithelial cell apoptosis index in the three groups were O,(5.58 ± 0.46)％ and (1.28 ± 0.24) ％.Comparison between sham operation group and model group,the expression of NF-κB,Bcl-2,ICAM-1 protein and cell apoptosis index was significant (all P ＜ 0.05);comparison between model group and experimental group,the diffrence of the factors was significant (all P ＜ 0.05).Conclusion Prostaglandin E1 had a protective effect on renal injury in rats with obstructive jaundice.The mechanism of the protective effect is related to inhibit the activation of NF-κB and decrease the expression of inflammatory factors.

Objective: To study the relevant risk factors of polycystic ovarian syndrome (PCOS) of Li People so as to provide basis for early diagnosis and treatment of PCOS. Methods: With case-control study method, 285 cases of PCOS of Li People were as recruited case group, and 580 cases of non-PCOS of female Li People as control group. Questionnaire was adopted to collect data regarding risk factors of PCOS, then the risk factors of PCOS was searched by univariate and multivariate analysis. Results: Multivariate analysis showed that the risk factors of PCOS included in menstrual cycle disorder (OR = 5.824), bad mood (OR = 2.852), family history of diabetes (OR = 7.008), family history of infertility (OR = 11.953), menstrual irregularity of mother (OR = 2.557) and lack of physical exercise (OR = 1.866). Conclusions: To target the high risk factors of menstrual cycle disorder, family history of diabetes, family history of infertility, family history of diabetes, bad mood and lack of physical exercise of female population, we should implement early screen, diagnose and treatment of POCS in order to reduce the incidence rate of PCOS and improve prognosis of PCOS.

"Karoshi" originates from Japan's economic take-off period in the 1960s and 1970s. It is generally believed that overwork lead to the accumulation of fatigue, which triggers the outbreak of potential diseases, and results in sudden death. Karoshi causes great harm to both the community and families because it occurs primarily in 30 to 60 year old young adults. Japan put Karoshi into the category of industrial injury for the first time in 2001 and started to undertake a series of studies in the sociological and pathological fields. However, there is a tremendous gap in the forensic pathological diagnosis domain. In China, research on Karoshi started from the 1990s and is closely related to the reform and opening up policy as well as economic development. According to the incomplete statistics, 600 thousand people die from overwork each year in China, the highest in the world. Karoshi has become one of the most serious social problems in China at the present stage, thus a systematic study in the sociology and forensic pathology fields is urgently required. This paper summarizes the past and present status of Karoshi, and puts forward the problems that need attention during the judicial expertise of Karoshi from forensic pathology perspective.
Adult ; China ; Death, Sudden/etiology* ; Fatigue/epidemiology* ; Forensic Pathology ; Humans ; Middle Aged ; Occupational Stress/epidemiology* ; Risk Factors

Acoustic Stimulation ; Brain Mapping ; Hallucinations ; Humans

Objective To study the effect of overwork stress response on the expression of connexin 43（Cx43） and connexin 45（Cx45） in cardiomyocytes and on cardiac function. Methods The experimental animals were divided into control group, overworked 1-month group and overworked 2-month group. A overworked rat model was established by forcing swimming of overworked group. The expressions of Cx43 and Cx45 in myocardial tissues of experimental animals were detected by Western blotting, while the corresponding myocardial tissues were stained with hematoxylin-eosin （HE） staining and Masson's staining, then histologically observed. Results Western blotting results showed that, compared with the control group, Cx43 expression in myocardial tissues of overworked rats decreased while Cx45 expression increased. HE staining and Masson's staining results showed that hypertrophy, rupture and interstitial fiber tissue hyperplasia were observed in myocardial fibers of overworked rats. Conclusion Overwork stress response may affect cardiac function as an independent factor and may even cause heart failure or arrhythmias and lead to death.
Animals ; Arrhythmias, Cardiac/metabolism* ; Connexin 43/metabolism* ; Connexins/metabolism* ; Heart Failure ; Myocardium ; Myocytes, Cardiac/metabolism* ; Rats