0.75).Conclusions The method of single-slice and multi-slice TSE sequence in T_2 measurement is feasible.For bone marrow and extremely long T_2 tissue(for instance,cerebrospinal fluid),however,there may be considerable discrepancy on T_2 values obtained by TSE sequence.

Humans ; Jaw, Edentulous ; therapy ; Maxilla ; Prostheses and Implants

Objective To study the expression of macrophage inflammatory protein 2(MIP-2) and the interfering effects of naloxone in the brain edema caused by lioposacchride (LPS)in rats.Methods Eithty-four SD rats were randomly divided into 3 groups:normal saline group(NS group,n=28) 0.2 mL normal saline was injected by carotid into each rat;LPS group(n=28) with 200 ?g LPS;naloxone interfering group(NAL group,n=28)1 mg/kg naloxone was intraperitoneally injected at 10 min,1,2,6,12 h and following LPS injected 2 h before decapitation.The content of MIP-2 and even blue(EB) in brain tissue were detected at different time point.The brain water content was measured by drying method.Results The content of water and EB in LPS group were significan higher than those in NS group(P

Objective To determine the diagnostic value of shoulder MR arthrography in revealing the injuries of anterior glenoid labrum,and to compare MR arthrography with arm in neutral position with MR arthrography with arm in abduction and external rotation(ABER)position.Methods MR arthrography of the shoulder,including additional oblique axial sequences with the patient in the ABER position,was performed in 44 patients.The injuries of anterior glenoid labrum of these patients were retrospectively evaluated.The result was compared with that of arthroscopy.Results For displaying the abnormalities of anterior glenoid labrum,the sensitivity,specificity,and accuracy of axial MR images with arm in neutral position were 79.3%(23/29),100%(15/15),and 86.4%(38/44),respectively.While the results of ABER position oblique axial images were 93.1%(27/29),100%(15/15),and 95.5%(42/44), respectively.The difference of sensitivity between axial and ABER-position scans was statistically significant (P

70 bpm during scan),the proportion of segments that could not be assessed because of motion artifact were 0.1%(1/759),1.1%(7/649),2.5% (10/407),42.6%(103/242),and 75.5%(108/143),respectively.With conventional selective coronary angiography as the golden standard,the sensitivity,specificity,positive and negative prediction values to detect≥50% stenotic lesions in the assessable segments were 79.2%,96.0%,83.8%,and 94.6%,respectively.There was a significant correlation between the number of segments per patient not assessable because of motion artifact and heart rate during the scan(r=0.655,P=0.000).Conclusion MSCT is capable of achieving high accuracy for detection of coronary artery stenosis,and is a reliable technique to diagnose coronary artery disease.

OBJECTIVETo explore the protective effect of recombinant beta-defensin-2 (BD2) on acute lung injury induced by sepsis in rats.

METHODSRecombinant adenovirus carrying an expression cassette of rat BD2 (rBD2) or control adenovirus carrying empty vector was administered intratracheally to Sprague-Dawley rats 48 h before acute lung injury, which was induced by cecal ligation and double puncture (CLP). Histological changes of the lungs and 7-day survival rate were determined.

RESULTThe alveolar damage, interstitial edema, and infiltration of neutrophils were reduced and the survival rate was increased significantly in BD2 group than those in control group (P < 0.05). The CFU of abdominal bacteria was comparable to that in the control rats (P > 0.05).

CONCLUSIONThe administration of recombinant BD2 protects lung injury and improves the survival of rats with induced sepsis.

Acute Disease ; Adenoviridae ; genetics ; Animals ; Genetic Therapy ; methods ; Genetic Vectors ; Lung Diseases ; etiology ; therapy ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Recombinant Fusion Proteins ; genetics ; physiology ; Sepsis ; complications ; Survival Analysis ; beta-Defensins ; genetics ; physiology

OBJECTIVETo explore the possibility of stable expression of cationic peptide beta-defensin-2 in eukaryocytes with adenovirus vector.

METHODSThe rat beta-defensin-2 (rBD2) gene was cloned at the downstream of CMV promoter of the adenoviral shuttle plasmid pShuttle-CMV. Then pShuttle-CMV-rBD2 was transformed into E. coli BJ5183-AD-1, in which recombination occurred between plasmids and pAdEasy-1 to construct pAdEasy-rBD2. After confirmation by endonuclease, linear pAdEasy-rBD2 was transformed into 292 cells to obtain packaged adenoviral expression vector, which was used to infect cos-7 cells and to establish respiratory adenovirus infection model of rat. The in vivo and in vitro expression activity of recombinant adenovirus was detected by Western blot and immunohistochemistry.

RESULTThe inserted DNA of pShuttle-CMV-rBD2 consisted of rat beta-defensin-2 gene. The pathological effect of infected cells, electronic microscopic observation and PCR showed that the recombinant adenovirus vector was constructed successfully. The concentration of the adenovirus was 10(9) PFU/ml. The vector expressed rat beta-defensin-2 efficiently in vivo and in vitro.

CONCLUSIONThe recombinant adenovirus vector can express cationic peptide beta-defensin-2 in eukaryocytes.

Adenoviridae ; genetics ; Animals ; Blotting, Western ; COS Cells ; Cell Line ; Cercopithecus aethiops ; Eukaryotic Cells ; metabolism ; Gene Expression ; Genetic Vectors ; genetics ; Humans ; Immunohistochemistry ; Male ; Rats ; Rats, Sprague-Dawley ; Recombinant Fusion Proteins ; genetics ; metabolism ; beta-Defensins ; genetics ; metabolism

OBJECTIVETo investigate the relationship between the contour of articular eminence and traces of the condylar kinematic center during jaw opening movement in healthy subjects, and compare trace characteristics of condylar kinematic center and MRI findings in TMD patients.

METHODSIn 10 healthy subjects, jaw-opening motion was recorded. The kinematic center and terminal hinge axis point of the condyle were used as trace reference points. The contour of articular eminence was examined by MRI. Seven patients with TMD signs and/or symptoms (disk displacement) were selected for this study. The condylar trace was recorded during jaw protrusion and opening-closing. The internal derangement in temporomandibular joints was detected by MRI and defined as: (1). normal disk position, (2). disk displacement with reduction, (3). disk displacement without reduction.

RESULTSIn healthy subjects, most of the opening traces of the kinematic center coincided with the contour of articular eminence (8/10 joints in left, 9/10 joints in right). For terminal hinge axis point, no trace coincided with the contour of articular eminence (0/20 joints in left and right). In TMD patients, according to MRI findings, the condylar traces of kinematic center in 3 normal disk position joints showed normal shape. However, in 6 disk displacements with reduction joints and 5 disk displacement without reduction joints, the condylar traces of kinematic center showed irregular patterns except 1 disk displacement with reduction joint.

CONCLUSIONSIn comparison with the terminal hinge axis point, the opening traces of the kinematic center can be interpreted as the translatory movement of the condyle/disc along the articular eminence. The study suggests the use of kinematic center in condylar movement studies.

Adolescent ; Adult ; Humans ; Magnetic Resonance Imaging ; Mandibular Condyle ; physiology ; Temporomandibular Joint ; physiology ; Temporomandibular Joint Disorders ; physiopathology

Objective To clarify the expression levels of miR-934 and miR-1307 in serum of patients with esophageal squamous cell carcinoma(ESCC),and analyze the relationship between their expression and clinicopathological features and prognosis.Methods A total of 125 ESCC patients(ESCC group)who were admitted to our hospital from January 2018 to December 2019 were selected,and 100 healthy subjects(control group)were selected during the same period,the expression of miR-934 and miR-1307 in serum were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR).After 3 years of follow-up,the patients were divided into miR-934 low expression group(＜2.53)and miR-934 high expression group(≥2.53),miR-1307 low expression group(＜2.10)and miR-1307 high expression group(≥2.10),based on their mean values,the prognosis of patients with different expression of miR-934 and miR-1307 in serum was compared,and the related factors affecting the survival and prognosis of patients were analyzed by univariate and multivariate COX regression analysis.Results The relative expression levels of serum miR-934 and miR-1307 in ESCC group were 2.53±0.72 and 2.10±0.68 respectively,significantly higher than those in control group's 1.32±0.39 and 1.12±0.33(all P＜0.05).Serum miR-934 and miR-1307 were closely related to TNM stage,lymph node metastasis,tumor differentiation and distant metastasis in ESCC patients(all P＜0.05).The 3-year progression-free survival(PFS)in serum miR-934 and miR-1307 high expression group was lower than that in miR-934 and miR-1307 low expression group.Multivariate Cox regression analysis showed that,TNM stage Ⅲ～Ⅳ,high expression of miR-934 and high expression of miR-1307,low differentiation degree and distant metastasis were independent risk factors for PFS in ESCC patients(all P＜0.05).Conclusion Serum miR-934 and miR-1307 are abnormally highly express in ESCC patients,and their high expression is an independent risk factor for poor prognosis of ESCC patients,which is expected to be a marker for prognosis evaluation of ESCC.

OBJECTIVETo investigate the effects of cis-combretastatin-A1 phosphate (cis-CA1P) on tumor cell proliferation, and its effects on the blood vessel formations.

METHODSMTT and IC50 values were used to assess the inhibitory effects of cis-CA1P on tumor cell proliferation. Chicken embryo chorioallantoic membrane and thoracic aorta annulations isolated from rats were used to investigate the effects of cis-CAIP on the blood vessel formation.

RESULTSCis-CA1P concentration-dependently inhibited the proliferations of several cancer cell lines, including human gastric carcinoma cell line MGC-803, human leukemic monocyte lymphoma cell line U937, human melanoma cell line A375, human colon cancer cell line HCT116, human breast carcinoma cell line MDA-MB-231, and human leukemia cell line K562. Cis-CAIP significantly decreased the formation of blood vessels in chicken embryo chorioallantoic membrane and in thoracic aorta annulations.

CONCLUSIONCis-CA1P inhibits cancer cell proliferation and prevents blood vessel formation.

Animals ; Aorta ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Chick Embryo ; Chorioallantoic Membrane ; blood supply ; drug effects ; Humans ; In Vitro Techniques ; Neovascularization, Pathologic ; prevention & control ; Phosphates ; pharmacology ; Rats ; Stilbenes ; chemistry ; pharmacology