1.Bacterial Endotoxins Test for Ilex Pubescens Compound Injection
Yi ZHUO ; Yue HUANG ; Yilu CHEN
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(02):-
Objective To establish a method for the determination of bacterial endotoxin in Ilex pubescens compound injection(LPCJ) .Methods According to China Pharmacopoeia (second part,2000),interference test was carried out to detect the endotoxins in LPCJ with limulus lysate agents (?was 0.25 EU/mL).Results Inhibition action was found in bacterial endotoxin test for LPCJ and in the 10-,20-,30-and 40-fold diluted liquid of LPCJ.It was proved that the maximum noninterference concentration was the 50-fold dilution.The inspection results are the same as that of rabbit pyrogen test.Conclusion The 50-fold diluted LPCJ is suitable for the determination of bacterial endotoxin and can be used as the quality control standard for its preparation.
2.Research on Teaching Innovation of Infectious Disease
Chao ZHUO ; Wenxiang HUANG ; Ze YUAN
Chinese Journal of Medical Education Research 2003;0(02):-
The problems in teaching infectious disease were discussed .The measures should be taken to improve the teaching quality from the varies of aspects, such as the aim and the model of the teaching, the arrangement of curricula, the cultivation of the clinical ability, and the method of examination and the application of multi-media materials.
3.Developing Pharmacists' Clinical Thinking with Clinical Pharmaceutical Practice as the Core
Jin HUANG ; Zhuo WANG ; Jinhong HU
China Pharmacy 2007;0(35):-
OBJECTIVE:To promote the development of pharmacists' clinical thinking ability.METHODS:By analyzing the differences between physicians and pharmacists in clinical thinking together with our practical experience,we expounded the key points of pharmacists' clinical thinking,i.e.critical thinking,comprehensive thinking,evidence-based thinking,patient-cored thinking and creative thinking.RESULTS & CONCLUSION:The clinical thinking that is in line with pharmacists' characteristics should be developed with clinical pharmaceutical practice as the core and it can be achieved by enhancing pharmacists' ability to detect and solve the drug related problems.
4. Biflavans or biflavons from stems of Daphne acutiloba
Chinese Traditional and Herbal Drugs 2013;44(14):1887-1892
Objective: To study the chemical constituents from the stems of Daphne acutiloba. Methods: The constituents were separated by column chromatography and their structures were elucidated by spectral data analyses. Results: Fourteen biflavans or biflavons were isolated from the EtOAc fraction of 95% ethanol reflux extract in the stems of D. acutiloba and were identified as daphnodorin A (1), daphnodorin C (2), daphnodorin C' (3), daphnodorin F (4), daphnodorin E (5), wikstrol A (6), daphnodorin K (7), iso-chamaejasmin (8), (+)-chamaejasmin (9), daphnodorin D1 (10), daphnodorin H (11), 3-methoxyl daphnodorin H (12), daphnodorin K' (13), and daphnodorin B (14). Conclusion: All the compounds except 14 are obtained from the stems of this plant for the first time.
5.Saponins Composition of Both Panax Notoginseng and Aplysia Neural Connective Analyzed with Matrix-assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry
Lijian FENG ; Lin HUANG ; Huiqin ZHUO ; Heqing HUANG
Chinese Journal of Analytical Chemistry 2009;37(12):1727-1732
Mixed saponin molecules in the extraction of Panax notoginseng(PNE) can be effectively desorpted into the molecular ionization for measurement and analysis by mass analyzer from matrix assisted laser desorption ionization-time of flight-mass spectrometry(MALDI-TOF MS). The saponin samples with chromatography purity were directly analyzed by MALDI-TOF MS, which indicated that the sensitivity of the method was higher than that of RP-HPLC. A technology of MALDI-TOF MS was directly employed to analyze the saponin kinds and their relative contents in Panax notoginseng(PN) while the saponins were perfectly extracted from the Chinese traditional medicine of PN. It was indicated that there were at least 20 saponins consisting of different molecular structure and that the content of both ginsenoside Rg1 and notoginsenoside R1 in PNE was relative high. R1 saponin was extracted and identified to follow its metabolism pathway by both thin layer chromatography and MALDI-TOF MS, respectively. The saponin fingerprinting maps in PNE can be established to evaluate the quality of PE and to study both metabolism pathway and mechanism of extra minim saponins in vivo.
6.Clinical observation of cut healing with gelatin paste after cervical intraepithelial neoplasia surgery by LEEP
Yao CHEN ; Jing ZHUO ; Chun LI ; Mingtong HUANG ; Huijun HUANG
Chinese Journal of Primary Medicine and Pharmacy 2008;15(9):1409-1410
Objective To observe the poet-operative clinical inpact on cervical intraepithelial neoplasia surgery by Loop elcctrosurgical excision procedure(LEEP),gelatin paste to improve the healing of the Cut,prevent the post-operative bleeding,and find out effective methods of preventing LEEP complications.Methods 100 patients with cervical intraepithelial neoplasia underwent LEEP in clinic.Divide them into two groups randomly,60 patients were adopted gelatin paste post-LEEP ag the cure group.while 40 patients were adopted as control group.Observation Vaginal bleeding,infection and cervical rear in short-term.Results In 60 patients of cure group,never patients with vaginal bleeding,infection and the cut healing in the short time.In 40 patients of control group,three patients with vaginal bleeding,two patients with infection,and three patients with bad healing.The different between two group with vaginal bleeding,infection and the cut healing time was significant(P<0.05).Condusion gelatin paste can reduce bleeding.infection and cervical repair bad cmpllcations after cervical intraepithelial neoplasia surgery by LEEP.
7.Scutellarin antagonizes ischemia-reperfusion injury to human cardiac microvascular endothelial cells by increasing ERK phosphorylation
Xian HUANG ; Xiaoxue LI ; Zhuo YU ; Weimin YANG ; Lin LI
Journal of Medical Postgraduates 2017;30(9):912-916
Objective Scutellarin (SCU), a Chinese traditional medicine, has a protective effect against ischemia-reperfusion (IR) induced myocardial injury, but it is not yet clear whether SCU acts against vascular endothelial IR injury via extracellular signal-regulated kinase 1/2 (ERK1/2).The aim of this study was to explore the effect of SCU on hypoxia-reoxygenation (HR)-induced injury to human cardiac microvascular endothelial cells (HCMECs) and its influence on the ERK1/2 signaling pathway.Methods HCMECs were subjected to normal culture and divided into a normal control, a DMSO, an SCU 1 μmol/L, and an SCU 10 μmol/L group.The model of HR injury was established by exposing the HCMECs to 12-h hypoxia and 12-h reoxygenation after treated with DMSO or SCU at 1 and 10 μmol/L for 2 hours.Then, the survival rate of the HCMECs was detected by MTT and trypan blue staining, the concentration of malondialdehyde (MDA) in the cells measured, and the expressions of the p-ERK1/2, ERK2 and GAPDH proteins determined by Western blot.Results SCU at 1 and 10 μmol/L significantly increased the survival rate of the normally cultured HCMECs ([110.40±2.34] and [122.00±1.25] %) as compared with that of the normal control (100%) (P<0.05), while HR injury markedly decreased the vitality of the HCMECs ([68.00±4.06] %) in comparison with that of the blank control (100%) (P<0.05).The survival rate of the HCMECs was remarkably higher in the HR+SCU 1 μmol/L and HR+SCU 10 μmol/L groups than in the HR model group ([90.53±3.67] and [92.04±2.32] %) (P<0.05), and so was their vitality in the SCU 10 μmol/L group than in the normal control ([96.78±2.01] vs [90.06±1.85] %, P<0.01), while their survival rate was significantly lower in the HR model than in the blank control ([73.72±4.91] vs [91.83±2.34] %, P<0.01) and remarkably higher in the SCU 10 μmol/L ([87.59±2.64] %) than in the HR model group (P<0.05).The MDA concentration in the HCMECs was markedly increased in the HR model and HR+DMSO groups as compared with the blank control (P<0.01), but decreased in the HR+SCU 1 μmol/L and HR+SCU 10 μmol/L groups in comparison with the HR model group (P<0.05).The expression of the p-ERK1/2 protein was significantly down-regulated in the HR model group as compared with the blank control (P<0.01), but up-regulated in the HR+SCU 10 μmol/L group in comparison with the HR model (P<0.01).Conclusion HR injury reduces the vitality of HCMECs, increases the MDA concentration, and down-regulates the expression of the p-ERK1/2 protein in HCMECs, while SCU acts against ischemia-reperfusion injury to HCMECs by increasing ERK phosphorylation.
8.Influence of family nursing intervention on daily life ability of senile dementia patients
Fengying LING ; Xin ZHUO ; Yangyu CHEN ; Hongyuan HUANG
Modern Clinical Nursing 2013;(5):45-46,47
Objective To investigate the influence of family nursing intervention on daily life ability of senile dementia patients? Methods Eighty senile dementia patients were equally randomized into two groups: the control group and the observation group? The controls received conventional home nursing instruction and health education and those in the latter group were managed with home nursing intervention? The activity of daily living scale(ADL)was used to assess their daily life ability? Result After nursing intervention,the daily life ability of observation group was better than control group(Z = 18?914,P < 0?05)? Conclusions The family nursing intervention is effective in directing the dementia patients with exercises of daily living ability? Thus it may improve their ability in daily life?
9.Efficacy of integrated swallowing function rehabilitation training in patients with nasal feeding during acute ischemic stroke
Zhuo WANG ; Weiqun SONG ; Yaping QU ; Xiaoqin HUANG ; Ling WANG
Chinese Journal of Cerebrovascular Diseases 2014;(7):342-346,353
Objective To investigate the effect of integrated swallowing function rehabilitation training on nasal feeding patients with pseudobulbar palsy or bulbar palsy after acute ischemic stroke. Methods A total of 54 patients met the diagnosis criteria were enrolled prospectively. They were divided into either an integrated group (n=31)or a control group (n=23)using a random number table method. The patients of the control group received routine treatment of stroke,including brain protection therapy,improvement of cerebral circulation,dehydration,regulating blood pressure,and symptomatic treatment,etc;in addition to conventional treatment,those of the integrated group also received integrated swallowing function rehabilitation training,including neuromuscular electrical stimulation plus swallowing reflex facilitation technique,and oral facial glossopharyngeal function training,etc. The oral intake function in patients of the integration and pseudobulbar palsy or bulbar palsy (according to MRI and signs)was compared between the two groups. The evaluation index was the Functional Oral Intake Scale (FOIS)score. Results (1)The FOIS score (median 4)of the integrated group was higher than that of the control group (median 2). There was significant difference between the two groups (P<0. 01). There was also significant difference before and after comparison between the two groups. (2)The FOIS score of pseudobulbar palsy in the integrated group was higher than that of the control group (median scores 4 and 2 ). There was significant difference (P<0.01);There was no significant difference in the FOIS scores of bulbar palsy between the two groups,but the FOIS score of the integrated group was higher than that of the control group (median scores 4 and 3). (3)The median differences between the integrated group and the control group were 2 and 1 respectively. The increased amplitude of FOIS of the integrated group was higher than that of the control group. There was significant difference (P<0. 01). Conclusion The integrated swallowing function rehabilitation training can improve the oral intake function,significantly reduce irritating cough,increase the capacity of oral intake,and increase the types of food. The effect of the improvement of pseudobulbar palsy is more significant in the integrated group.
10.Analysis of 8 Children with Familial Periodic Paralysis and Literature Review
bo, LI ; wei-hua, ZHUO ; ruo-gu, HUANG
Journal of Applied Clinical Pediatrics 2006;0(14):-
Objective To understand well this disease,8 children with familial periodic paralysis(FPP) were reported and the(rela)-ted literatures were reviewed.Methods The hereditary characters,clinical manifestations,auxiliary examination and managements were summarized retrospectively in 8 cases of FPP patients hospitalized from January 1996 to December 2005,and etiopathogenis and diagnosis were also analyzed.Results Six cases of FPP were diagnosed as hypokalemic periodic paralysis,and all occured as an autosomal dominant condition.During paralytic episodes,the patients showed obviously low serum potassium levels [1.9-2.8 mmol/L,(2.4?0.38) mmol/L)] and hypokalemic electrocardiogram findings,such as U-wave.The level of blood glucose was lower than normal range.Other 2 cases with normal serum potassium ion level at attack were diagnosed as normokalemic periodic paralysis with autosomal dominant pattern.One of the two cases,the level of blood glucose was lower.Thyroid functions,renal functions and electromyograms were all normal in 8 cases.Conclusions FPP is a group of relatively uncommon inherited disorders known as the skeletal muscle channelophathies.It can be diagnosed by hereditary characters,clinical manifestataions,auxiliary examinations.