Objective To compare and determine the reproducibility of apparent diffusion coefficient (ADC)values of the normal liver parenchyma in respiratory-triggered,breath-hold,and free-breathing diffusion weighted magnetic resonance imaging (DWI). Methods 11 volunteers did three times of breath-hold (excitation number is 1,at the end of the expiration,thickness/gapping=5 mm/1 mm), respiratory-triggered (excitation number is 2,at the end of the expiration,thickness/gapping=5 mm/1 mm),free-breathing (excitation number is 4,thickness/gapping=5 mm/1 mm)and for a total of nine axial liver DWI,the gradient factor was 800 s/mm2 .Then they were all coronally reconstructed (thickness/gapping=8 mm/1 mm).The nonparametric test was used to compare all axial and coronally reconstructed liver ADC values;and the Bland-Altman method was used to evaluate the ADC values’repeatability in coronally reconstructed images.Results The breath-hold DWI’s mean ADC value was (1.09±0.07)×10-3 mm2/s significantly lower than the respiratory-triggered DWI(1.16± 0.09)×10-3 mm2/s and the free-breathing DWI(1.19±0.10)×10-3 mm2/s(P<0.005),the axial and coronal images’results were similar.The ADC values’average difference and scope of consistency of coronal image were (-0.09 to -0.01±0.09 to 0.48)for breath-hold DWI,(-0.04 to 0.01±0.11 to 0.17)for respiratory-triggered DWI,(-0.07 to -0.02±0.11 to 0.18)for free-breathing DWI,respectively.Conclusion The breath-hold DWI’s ADC value of normal liver parenchyma is lower than the respiratory-triggered and free-breathing DWI,and showed more poorer repeatability.It is suggested that the measurement of ADC values of the liver parenchyma should be done within the respiratory-triggered or free-breathing DWI.

Objective:To investigate the infuluence of electroacupuncture on blood lipid and liver function in nonclcoholic fatty liver rats. Methods:Thirty-three male SD rats were randomly divided into 2 groups:normal control group (n=11,fed with normal food) and non-alcoholic fatty liver model group (NAFLD) (n=23,fed with high-fat food). Eight weeks later 1 rats were randomly selected from the normal control group and 2 rats were randomly selected from the NAFLD group and were killed to undergo pathological examination of the liver. When the establishment of experimental model of NAFLD rats was confi rmed the remaining 20 NAFLD rats were sublishment into 2 equal subgroups:NAFLD control group to be fed continuously with high-fat food ,acupuncture treatment group (to fed continuously with high-fat food). Take Zusanli,Hong Leong,Sanyinjiao,Taichong points,electroacupuncture -spacing parameters,2Hz frequency,intensity 4mV to the appropriate vibrating slightly lower limb. By the end of the 12th week,all rats were killed to isolate the samples of test the levels of serum total cholesterol (TC),serum triglyceride (TG),high-density lipoprotein cholesterol (HDL-L),low-density lipoprotein cholesterol (LDL-CH),liver index,Valley serum ALT(ALT),Aspartate aminotransferase (AST),and liver pathology. Results:Treatment of rats --serum total cholesterol (TC),serum triglyceride (TG),low-density lipoprotein cholesterol (LDL-CH),liver index,Valley serum ALT(ALT),Aspartate aminotransferase (AST) were significantly lower than those of NAFLD control group (P

Objective To explore the activities of daily living (ADL) and related factors of patients with Parkinson's disease (PD). Methods118 PD patients were assessed with the ADL scale, Unified Parkinson Disease Rating Scale-Ⅲ (UPDRS-Ⅲ), the Hamilton Rating Scalefor Anxiety (HAMA) and Hamilton Rating Scale for Depression (HAMD). Results ADL score in all the patients was (40.75±14.63); ADLscore was higher in anxiety or depression group than in non-anxiety or non-depression group respectively (P<0.05). Scores of UPDRS-Ⅲcorrelated with the ADL (r=0.506, P=0.000). Conclusion ADL decreases in PD patients, especially those with anxiety and depression. Themotor disorder of PD patients correlated with ADL.

Published reports of benign nerve sheath tumors of the oropharynx especially at tongue base are extremely rare. They may slowly growing but represent a potential threat to the airway, prompt diagnosis and treatment are vital. To our knowledge, this case presents the rare clinical condition and the first giant one. Due to the rarity, awareness of the possibility of a schwannoma in various otolaryngological conditions is an important step in making a correct clinical diagnosis. Here we represent a rare case of a giant schwannoma that arose from valleculae projecting to supraglottis to cause swallowing difficult but no breathing problem. The mass was completely removed, and no recurrence during next 2 years follow-up.
Humans ; Male ; Middle Aged ; Neurilemmoma ; Tongue Neoplasms

Objective To assess patterns of the gray and white matter atrophy in patients with multiple system atrophy-P(MSA-P)variant of whole brain compared with normal controls.Methods Three dimensional fast spoiled gradient echo(3D-FSPGR)T1WI of whole brain were obtained from 13 patients with probable MSA-P and 14 age-matched normal controls.The volume of gray matter(GM)and white matter (WM)of MSA-P patients and normal controls was analyzed with voxel-based morphometry(VBM) using statistical parametric mapping(SPM)8.Results Compared with the controls,the MSA-P patients showed decreased gray matter and white matter in bread areas. Gray matter loss mainly symmetrically distributed in bilateral supplementary motor area(SMA),dorsal posterior cingulate cortex(DPCC),medial frontal gyrus,superior temporal gyrus,cerebellum cortex,etc.Unilateral involvement of cortices mainly located in right primary motor cortex.somatosensory association cortex(SAC),and left ventral anterior cingulate cortex (VACC).There Was white matter loss in bilateral superior frontal gyrus,bilateral precuneus,bilateral subgyrus of frontal lobe,left superior temporal gyrus,left cingulate gyrus,right orbitofrontal area,right subgyrus of temporal lobe,etc.Conclusion VBM method is an automatic and comprehensive volumetry method and Can objectively detect the difference of the whole brain structure in patients with probable MSA-P comparing with normal controls.

Objective To investigate the clinical significance of NKp44+NK cells in the peripheral blood and synovial fluid of rheumatoid arthritis (RA) patients.Methods The proportions of NKp44+NK cells in the PB of 20 active and 15 remission patients with RA and 20 healthy individuals were detected using flow cytometry.The proportions of NKp44+NK cells in the SF of 10 active patients were detected.Multiple nonparametric test was used to compared the difference of NKp44+NK cells proportions.Clinical data including DAS28,anti-CCP antibody and RF were collected.The relationship between NKp44+NK cells and clinical data was analyzed by Spearman correlation analysis.Results The proportions of NKp44+NK cells in PB of active and remission patients and healthy individuals were (1.480±4.750)%,(0.540±0.590)%,(0.000±0.000)%respectively.The proportion of NKp44+NK cells in PB of active patients was significantly higher than that of remission patients and healthy individuals (x2=46.708,P=0.000).The proportion of NKp44+NK cells in the SF of ten active RA patients was significantly higher than matched PB.There was positive correlation between NKp44+NK cells proportion in PB and SF of active patients and DAS 28 and anti-CCP antibody level.There was no correlation between NKp44+NK cells proportion in PB,SF and RF.Conclusion There is a marked increase in the proportion of NKp44+NK cells in PB and SF of RA patients.Moreover,NKp44+NK cells are positively correlated with DAS28 and anti-CCP antibody,suggesting that NKp44+NK cells may be correlated with disease activity and severity.

Objective Oral and Maxillofacial trauma Diagnosis and Treatment Expert system (OMDTES)was used in maxillofacial trauma PBL teaching to improve the quality of PBL teaching,and perfeet the assessment criteria, Methods OMDTES was applied in two procedures of maxillofacial injury PBL teaching activity:the preparation of lesson plan and assessment criteria.Then a questionnaire was designed to assess the effect of this new mode of PBL teaching.Results The new teaching model is welcomed by students and can improve students'interest in learning,the satisfaction of teaching and ameliorate method of assessment of PBL teaching.Conclusion Applying OMDTES in oral and maxinofacial injury PBL teaching has special advantages in raising the leavnevs'activity of learning,training their ability of chnical thinking and analysis.And it is worthy of further research and amelioration.

AIM: To investigate the clinical effect of vitamin B12 eye drops for vision fatigue caused by visual display terminals (VDT).
METHODS: Totally 50 patients (100 eyes) with vision fatigue caused by VDT were averagely divided into two groups. The control group were treated with normal saline,the treatment group were treated with vitamin B12 eye drops, 3 times per day, one drop each time, continuous for 60d. Accommodative parameters and Schirmer Ⅰtest were measured and analyzed before and after treatment.
RESULTS: After treatment, the results of Schirmer Ⅰtest, accommodative amplitude and accommodative facility of the treatment group were higher than those of the control group ( all P < 0. 05 ). And the results of accommodation lag of the treatment group were lower than those of the control group (P<0. 05).
CONCLUSION: Vitamin B12 eye drops can lessen symptoms of dry eye, improve accommodative function and treat vision fatigue caused by VDT.

Mixed saponin molecules in the extraction of Panax notoginseng(PNE) can be effectively desorpted into the molecular ionization for measurement and analysis by mass analyzer from matrix assisted laser desorption ionization-time of flight-mass spectrometry(MALDI-TOF MS). The saponin samples with chromatography purity were directly analyzed by MALDI-TOF MS, which indicated that the sensitivity of the method was higher than that of RP-HPLC. A technology of MALDI-TOF MS was directly employed to analyze the saponin kinds and their relative contents in Panax notoginseng(PN) while the saponins were perfectly extracted from the Chinese traditional medicine of PN. It was indicated that there were at least 20 saponins consisting of different molecular structure and that the content of both ginsenoside Rg1 and notoginsenoside R1 in PNE was relative high. R1 saponin was extracted and identified to follow its metabolism pathway by both thin layer chromatography and MALDI-TOF MS, respectively. The saponin fingerprinting maps in PNE can be established to evaluate the quality of PE and to study both metabolism pathway and mechanism of extra minim saponins in vivo.

BACKGROUND:Currently, human umbilical cord derived-mesenchymal stem cel s are mainly for local transplantation, which has some shortcomings, such as large trauma, bleeding, complications, that limit its widespread application in clinical practice. OBJECTIVE:To investigate the feasibility of intravenous transplantation of human umbilical cord derived-mesenchymal stem cel s for repair of spinal cord injury. METHODS:Eighty Wistar rats with spinal cord hitting were divided into five groups:blank control group with no transplantation (n=10), DMEM local transplantation group (n=15), DMEM intravenous transplantation group (n=15), cel local transplantation group (n=20), cel intravenous transplantation group (n=20). The functional recovery of spinal cord injury was observed with Basso, Beattie and Bresnahan scores at regular time as wel as hematoxylin-eosin staining and immunohistochemistry staining. RESULTS AND CONCLUSION:During 1 day to 2 weeks after transplantation, there was no significant difference in the Basso, Beattie and Bresnahan scores between the five groups;within 4-12 weeks after transplantation, the Basso, Beattie and Bresnahan scores were significantly higher in the two cel transplantation groups than the other three groups, but there was no difference between these two cel transplantation groups (P>0.05). Histological observation showed that the number of voids and glial scars was less in the cel local transplantation group and cel intravenous transplantation group compared with the other three groups, and there was also no difference between the two cel transplantation groups. These results indicate that the intravenous transplantation of human umbilical cord derived-mesenchymal stem cel s is similar to the local transplantation in the repair of acute spinal cord injury, which is simple and avoids secondary injuries and various complications. It is recommended that this method provide a new approach for cel transplantation.