Objective To investigate the quality of life ( QOL ) in ophthalmologic inpatients with common diseases , and to analyze the relation between QOL and visual function and the related influence factors . Methods One hundred and twenty-eight ophthalmologic inpatients were surveyed by the America National Eye Institute 25-Item Visual Function Questionnaire ( NEI-VFQ-25 ) , and the related influence factors were analyzed.Results The average score of NEI-VFQ-25 in patients was (58.1 ±22.0).The average score of total health status was (40.8 ±24.3), and the score in bilateral blind patients was (18.8 ±11.0), and the score in unilateral blind patients was (52.4 ±18.1), and the score in non low vision patients was (69.6 ±18.3), and the score in unilateral low vision patients was (60.2 ±21.2), and the score in bilateral low vision patients was (52.2 ±23.8).The difference was found in the score of QOL in different age and vision patients (F=4.966, 8.557, respectively;P<0.01).The vision of good eye was negatively related to the age (r=-0.306,P<0.01).Conclusions The vision and age can affect the score of scales , and the score of bilateral blind patients is significantly decreased .The nurses should correctly assess self-care ability of inpatients , and provide the necessary physical and mental nursing , and do conduct propaganda well so as to improve the QOL in patients .

OBJECTIVETo screen rare blood groups Fy(a-), s-, k-, Di(b-) and Js(b-) in an ethnic Zhuang population.

METHODSSequence-specific primers were designed based on single nucleotide polymorphism (SNP) sites of blood group antigens Fy(b) and s. A specific multiplex PCR system I was established. Multiplex PCR system II was applied to detect alleles antigens Di(b), k, Js(b)1910 and Js(b) 2019 at the same time. The two systems was were used to screen for rare blood group antigens in 4490 randomly selected healthy donors of Guangxi Zhuang ethnic origin.

RESULTSWe successfully made the multiplex PCR system I. We detected the rare blood group antigens using the two PCR system. There are five Fy(a-), three s(-), two Di(b-) in 4490 Guangxi zhuang random samples. The multiplex PCR system I has achieved good accuracy and stability. With multiplex PCR systems I and II, 4490 samples were screened. Five Fy(a-), three s(-) and two Di(b-) samples were discovered.

CONCLUSIONMultiplex PCR is an effective methods, which can be used for high throughput screening of rare blood groups. The rare blood types of Guangxi Zhuang ethnic origin obtained through the screening can provide valuable information for compatible blood transfusion. Through screening we obtained precious rare blood type materials which can be used to improve the capability of compatible infusion and reduce the transfusion reactions.

Blood Group Antigens ; genetics ; Duffy Blood-Group System ; genetics ; Genotype ; Humans ; Multiplex Polymerase Chain Reaction ; methods ; Receptors, Cell Surface ; genetics