Objective To explore the eardrum massage to reduce the effect of middle ear barotrauma after hyperbaric oxygenation therapy. Methods A total of 160 hospitalized patients with middle ear barotrauma after hyperbaric oxygenation therapy at first time were averagely divided into experimental group and control group by random number table. Valsalva maneuver had been used in the control group while eardrum message were used in the experimental group guided by pneumatic otoscope. Two groups were observed in patients with ear plug, earswelling, earache, the time of above symptom lighten and the time of eardrum improvement. Results In the observation group, the alleviated time of ear plugging time, earache and ear swelling were shorter than that of control group (t = 11. 304,8. 270,8. 640;P < 0. 01). For the I grade tympanic membrane injury, the healing time was shorter in the experimental group compared with that of the control group ( t =2. 095,P < 0. 05). Conclusions After hyperbaric oxygenation therapy, the symptom of ear plug, ear swelling, earache and tympanic hyperaemia are used eardrum message to promote blood circulation and negative pressure of middle and external ear, so as to minimize the discomfort of ear.