OBJECTIVE:To explore the lessons of plate fixation failure and the experiences of retreatment. METHODS:Computer-based online search of CNKl,CBM,VlP,Wiley,Elsevier,Springer and Kluwer databases were performed with of "plate,failure,breakage" to retrieve literature (1999/2008). RESULTS:The risks of plate fixation failure were hidden in the whole procedure of fracture treatment and rehabilitation course. The integrated evaluation and composite methods should be used in retreatment. CONCLUSION:Strict interal fixation indication,resonable internal/external fixation methods should be emphasized on fracure treatment. Precise principles understanding,perfect operative skills,biological osteosynthesis methods should be emphasized on plate implanting. A step-by-step rehabilitation course and regular follow-up should be emphasized on post-operation. A bone graft plus vascularized periosteal flap transplanting method would greatly increase the bone union rate in retreatment.

Objective To explore the incidence,mechanism and type of attack,relationship between hemorrhage postitions and manifestation,and its prognosis of eplieptic seizure following intracerebral hemorrhage(ICH) in the elderly.Methods Clinical materials are analyzed in 47 elder patients with epileptic seizures following ICH.Results The incidence of epileptic seizures following ICH in the elder patients is 9 6% .Early seizures are related to brain edema;most of them were partial seizures.But late seizures were related to the pathologic stimulation of stroke cyst,neuron degeneration,colloid hyperplasia and conglutination.Generalized tonic-chronic seizure plays an important role in it.Most seizures are between 2 weeks and 6 months after hemorrhage.The incidence of epileptic seizure following lobar hemorrhage was higher than others.Late seizures recurs easily if stopping antiepileptic drugs(AED).The disease's prognosis is well if treated regularly and systematically.Conclusiion Epileptic seizures following ICH are not rare in elder patients .Lobar hemorrhgae easy to lead to epileptic seizures.Regular and systimatic treatment is needed in the patients with late manifestation.

Objective To study the apoptosis of peripheral blood lymphocyte cells in patients with HAM/TSP and the roles of Fas, TNF-? and Bcl-2 in occurrence and progression of HAM/TSP.Methods The single cell gel electrophoresis (comet assay) was used to detect the apoptosis of lymphocyte cells in 8 patients with HAM/TSP, 8 cases with other inflammatory neurologic diseases (OIND) and 8 healthy blood donors. The Fas, TNF-? and Bcl-2 protein expressions were quantitatively determined by flow cytometry from 8 cases of HAM/TSP.Results The apoptotic ratio of lymphocyte cells in patients with HAM/TSP significantly increased compared with the controls (P

OBJECTIVE:To observe the clinical effects of Stronger Neo-Minophagen C(SNMC)in the treatment of liver function damage caused by anti-tumor medicine.METHODS:60cases of liver injury caused by anti-tumor medicine were randomly divided into the treatment group(32cases)and the control group(28cases).SNMC injection was given in the treatment group as a supplement to the primary therapy in the control group.RESULTS:The treatment group with SNMC showed significantly greater improvement than the control group in the liver functions(ALT,AST,T.BIL)(P

Objective To study the effects of propofol on the whole-cell sodium currents in rat sympathetic neurons in order to investigate the mechanisms of propofol-mediated peripheral vasodilation.Methods Whole-cell patch-clamp recordings were made from enzymatically isolated rat superior cervical sympathetic neurons.Results Propofol dose-dependently blocked the whole-cell sodium currents evoked by a voltage step from a holding potential of -- 80mV to 0mV with a mean IC50 value of 32. 19?mol/L (r = 0. 982, P

Objective To investigate the effects of ropivacaine on the whole cell sodium currents in rat sympathetic ganglion neurons in order to determine whether sympathetic ganglion is involved in the ropivacaine cardiotoxity. Methods The sympathetic neurons were enzymatically isolated from the superior cervical ganglion. The effects of ropivacaine on the whole cell sodium channel currents were assessed using patch clamp technique. Results Ropivacaine blocked the whole cell sodium channel currents dose dependently. When the holding potential (Vh) was -80mV and Vt Omv , 0.01?mol/L ropivacaine reduced peak currents by 30.02% with a mean IC50 of 2.68?mol/L. The blockade was also potential dependent. When Vh was -60mv, the mean IC50 was 1.55?mol/l. 1.0?mol/L ropivacaine reduced the peak value of I V curve by 30.66% but did not affect the shape of I V curve and caused 2.56mv shift of voltage dependence activation curve to depolarized potentials and 3.56mv shift of steady state inactivation curve to more hyperdepolarized potentials.The conditioning pulse potential at which half maximal channels were activated (V1/2), changed from -52.99mv to -56.44mv and the test potential at which half maximal channels were activated (V1/2), changed from -25.2mv to -22.64mv. Conclusions Subconvulsive concentration of ropivacaine significantly inhibits sympathetic ganglion neurons in a dose dependent and potential dependent way through the inactivation of sodium channel,indicating that sympathetic ganglion neurons may contribute to the cardiotoxity of ropivacaine.

ve To investigate the effects of clinical relevant concentrations of midazolam on whole-cell N-type calcium currents of sympathetic ganglion neurons trying to assess the involvement of sympathetic ganglion in circulation depression produced by midazolam. Methods 7-10 d SD rats were decapitated and bilateral superior cervical ganglions were rapidly removed and kept in 4℃, oxygen saturated incubation solution. TTX 1?mol/L and nifedipine 10 ?mol/L were added to the solution to block the Na+ and L-type calcium currents. Midazolam was also added to the solution. The final concentration of midazolam were 0.1, 0.3, 3, 10, 50, 100?mol/L.The effects of different concentration of midazolam on the whole-cell N-type calcium channel currents were assessed by patch-clamp technique. Results When the holding potential (Vh) was - 80mV and stimulating votage(Vt) was between - 30mV and 10mV midazolam inhibited the whole-cell N-type calcium currents dose-dependently ( r = 0.964, P

Biomarkers ; analysis ; Genomics ; methods ; trends ; Humans ; Preventive Medicine ; methods ; trends

Objective To investigate the clinical application of mutations drug-resistant tuberculosis (MDR-TB) by polymerase chain reaction(PCR)-oligonucleotide probes. Methods 80 cases of MDR-TB were divided into observed group 40 cases and conventional group 40 cases, the resistant gene and the type of mutation were determined by polymerase chain reaction (PCR)-oligonucleotide probes. Results 78 isolates of 4 kinds of resistance genes (KatG,inhA,rpoB,embB) ,the mutation rates were 90.9% ,84.8% ,51.98% and 58.1% ;the gene of rpoB sensitivi-ty of 98.0% and specificity of 100% ,positive predictive value of 82.61% ,the rate of according with traditional drug susceptibility testing were 62.50% by polymerase chain reaction (PCR)-oligonucleotide probes;the serisitivity of the KatG gene mutation is of 50.56% ,specificity of 75% ,positive predictive value of 68.75% ,the rate of according with traditional drug susceptibility testing were 46.43% by polymerase chain reaction (PCR)-oligonucleotide probes;the observed group in focus significant absorption, absorption, invariability, deterioration were 32.5% ,62.5% ,5.0%, 0% ,higher than the conventional group(25.0% ,50.0% ,20.0% ,5.0% ) (χ2=3.98,χ2=3.92, P<0.05;χ2=6.78,χ2=6.80,P<0.01) ;observed group in 3 months after treatment,6 months,9 months sputum the conversion negative rate in observed group (65.0%, 77.5%, 85.0% ) higher than the conventional group (37.5%, 50.0%, 60.0%) (χ2=3.86,χ2=3.85,χ2=3.89,P both<0.05). Conclusion An analysis available 3 kind of MDR-TB, 4 kind gene by PCR-oligonucleotide;the observed group in curative effect is better than the conventional group in pa-tients with MDR-TB.

BACKGROUND:SenseWear Pro Armband (SWA) is a monitoring device of physical activity energy consumption which consists of many kinds of motion sensors, and it has been widely used in the study of physical activity energy consumption in recent years. OBJECTIVE:To summarize the SWA accuracy, software version, time of wear as wel as its application in different populations and at different physical activity levels, for the purpose of providing some practical reference in the future usage of SWA. METHODS:A computer-based search of CNKI (ful-text database), PubMed and Web of Science databases was done for relevant articles published before January 2015 using the keywords of “physical activity, sensewear pro armband, energy expenditure, review” in Chinese and English, respectively. Articles published in core journals or SCI database were preferred. RESULTS AND CONCLUSION:Double labeled water method and indirect measurement method serve as standard criteria, and the relevant study shows that SWA has higher accuracy and can effectively evaluate the daily physical activity energy consumption. But under the special circumstances, there are some differences, such as different people and different exercise intensities. Meanwhile, to determine the appropriate wearing time and software version for data statistics are also one of the factors influencing the accuracy of the final result. SWA can accurately predict physical activity energy consumption, but for different populations and exercise intensities, it is necessary to improve the further calculation formula of SWA.