Objective: To investigate the interaction between fragile histamine triad (FHIT) and protein kinase C alpha (PKCα) in human non-small cell lung cancer tissues. Methods: FHIT and PKCα double positive samples were screened by immunohistochemical staining from 13 human non-small cell lung cancer tissues. Co-immunoprecipitation was performed by using anti-FHIT and anti-PKCα. The immune precipitate was analyzed by SDS-PAGE and Western blot. Results: Immune precipitate staining detection showed that 3 samples out of the 13 cases were double positive for FHIT and PKCα. FHIT protein was present in the immune precipitate of anti-PKCα while there was PKCα in the immune precipitate of anti-FHITmAb. Conclusion: FHIT and PKCα exist as a complex in human non-small cell lung cancer tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human non-small cell lung cancer.

Objective: To investigate the interaction between fragile histamine triad (FHIT) and protein kinase C alpha (PKCα) in human non-small cell lung cancer tissues. Methods: FHIT and PKCα double positive samples were screened by immunohistochemical staining from 13 human non-small cell lung cancer tissues. Co-immunoprecipitation was performed by using anti-FHIT and anti-PKCα. The immune precipitate was analyzed by SDS-PAGE and Western blot. Results: Immune precipitate staining detection showed that 3 samples out of the 13 cases were double positive for FHIT and PKCα. FHIT protein was present in the immune precipitate of anti-PKCα while there was PKCα in the immune precipitate of anti-FHITmAb. Conclusion: FHIT and PKCα exist as a complex in human non-small cell lung cancer tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human non-small cell lung cancer.

Objective To investigate comparability between Sysmex different hematology analyzers.The consistency of all he-matology analyzers with the same fresh sample.Methods Selected Sysmex XE-2100 as reference instrument which attend external quality assessment.Fresh high,medium and low blood were detected in Sysmex XS-800i,Sysmex XT-4000i and Sys-mex XT-1800i,compared results of WBC,RBC,HGB,HCT,MCV and PLT.Results The bias of XS-800i and XE-2100 was WBC 2.85%,RBC 1.44%,HGB 0.75%,HCT 2.11% and PLT 5.53%.The bias of XT-4000i and XE-2100 was WBC 1.26%,RBC 0.95%,HGB 0.68%,HCT 1.35% and PLT 2.68%.The bias of XT-1800i and XE-2100 was WBC 5.21%, RBC 1.96%,HGB 1.60%,HCT 1.96% and PLT 4.95%.It had good compatability.All various parameters was in the al-lowed range.Conclusion Should maintenance and compare the hematology analyzers periodically,found the problem then calibration instruments in time,ensure the consistency of measurement results between different instruments and guarantee accuracy of the results.

Objective To probe the effects of the size and location of cranial defect on the neurological symptoms of patients with skull bone defect.Methods The patients were classified into the following groups according to the influence to symptoms by changes in body position and changes of symptoms before and after craniectomy: True syndrome of the trephined(ST,Ⅰ), partial ST patients(Ⅱ), other patients or symptom-free patients (Ⅲ).Results The group I consisted of 33 cases, their defects were located in the parieto-occipital region in 23 cases, frontoparietal in 10 cases. All these patients had flaccid skin flaps that became concave while in the upright position. The group Ⅱ was composed of seven cases, their defects were located in frontoparietal and parieto-occipital region in three respectively. All these patients had flaccied skinflap in defects pars.Group Ⅲ included twelve patients, flat and rigid skin flaps were usually demonstrated , predominantly in the tempora and parietal regions.Conclusions The size and location of the cranial could play an important role in the development of the neurological symptoms.

Objective To investigate the therapeutical effects of somatostatin in nonoperative treatment of different kinds of intestinal obstruction.Methods 338 cases of intestinal obstruction accepted by our hospital were retrospectively analyzed,which were divided into adhesive intestinal obstruction,inflammatory intestinal obstruction and malignant tumor intestinal obstruction.With nonoperative treatment,the control group were treated with routine therapy,including fasting,gastrointestinal decompression,intestinal lavage,intravenous replacement of fluid and electrolytes,disturbance of acid-base balance and the application of antibiotics.As for the treatment group,they were pumped with 6mg of somatostatin and 120mL physiological saline as well as routine therapy for 24 hours.The effects of the two groups were compared.Results The remission rate of abdominal distension and abdominal pains was 80.2％ in the treatment group and 62.3％ in the control group,the difference was statistical significance (x2 =250.446,P ＜ 0.05).The remission rate of nausea and vomiting was 84.1％ in the treatment group and 87.7％ in the control group.The passage of gas and defecation by anus was 64.7 ％ in the treatment group and 61.6％ in the control group,there was no clear difference between the two groups (x2 =0.902,0.349,all P ＞ 0.05).The gastrointestinal decompression amounts reduced clearly,the treatment group was (328 ±229) mL/d and (694 ± 381) mL/d in the control group,there was significant difference (t =2.883,P ＜ 0.05).After nonoperative conservative treatment,the effects between the two groups were as follows:the effective percentage was 95.7％ in the treatment group and 94.0％ in the control group,there was no significant difference(x2 =0.007,P ＞ 0.05).Except intestinal obstruction patients with external tumors,the rates of transferring to operation was 2.4 ％ in the treatment group and 2.8 ％ in the control group,there was no statistical significance(x2 =0.051,P ＞ 0.05).And except patients of intestinal obstruction with external tumors and transferring to operation,the hospital stay was (10.6 ±4.9) d in the treatment group and (15.3 ± 4.1) d in the control group,the difference was significant (t =2.528,P ＜ 0.05).As for the treatment group with somatostatin,there was no significant difference(x2 =0.008,0.230,t =0.117,all P ＞ 0.05) in abdominal distension and abdominal pains of adhesive and inflammatory intestinal obstruction,promoting the passage of gas and defecation by anus,the improvement of gastrointestinal decompression amounts in 24 hours.The improvement of tumor intestinal obstruction was worse.Conclusion Somatostatin treatment can help to ease the symptoms and physical signs of different kinds of intestinal obstruction rapidly and cut down hospital stay clearly,but can not reduce the rates of transferring to operation.If the time of treatment is ignored,it can not improve the therapeutical effective percentage of intestinal obstruction obviously.There is no significant difference for the application of adhesive and inflammatory intestinal obstruction.However,it can not completely and well improve the symptoms of tumor intestinal obstruction and the symptoms can recrudesce easily.

Objective To evaluate the levels of neutrophil gelatinase associated lipocalin (NGAL) in prediction of acute kidney injury (AKI) in children with burn. Methods Forty seven children with burn were selected in this study and thirty healthy children were enrolled as a control group. Levels of blood routine, urine routine, serum creatinine (Scr), serum NGAL (SNGAL), urine NGAL (UNGAL), blood urea nitrogen (BUN) and C-reactive protein (CRP) were measured. Receiver operating characteristic (ROC) curve were used to estimate the AKI diagnostic value of serum NGAL, urine NGAL and Scr. Results On the day of admission, serum NGAL and urine NGAL were significantly increased in patients with AKI , while Scr did not change significantly as compared with those in non-AKI patients and the healthy controls. In AKI group, serum NGAL and urine NGAL were positively correlated with WBC and CRP on the day of admission , and positively related with Scr on the seventh day. ROC analysis showed the AUC of serum NGAL, urine NGAL and Scr on admission were 0.737(95%CI: 0.653 ~ 0.779, P < 0.05), 0.772(95%CI: 0.632 ~ 0.793, P < 0.05), and 0.508(95%CI: 0.454~0.618, P < 0.05) respectively. The cut-off value of serum NGAL and urine NGAL were 318 μg/L (sensitivity of 72.8% and specificity of 81.6%) and 105 μg/L (sensitivity of 75.6% and specificity of 83.7%). Conclusions Elevation in levels of serum NGAL and urine NGAL occurs earlier than that in Scr level in children with burn. Both serum and urine NGAL level can be used as a biomarker for early prediction of AKI.

Humans ; Infant, Newborn ; Intestinal Perforation ; complications ; diagnosis ; Mycoses ; complications ; diagnosis

Objective To investigate the clinical application and therapeutic effect of reservation cortical expand resection and reconstruction for extremities bone tumor. Method Twelve cases with extremities invasion bone tumor were reviewed,including 6 male and 6 female.The average age was 37.1 years old.According to the MRI,TypeⅠtumor located in metaphysic and growth eccentricity,and was treated with outside tumor osteotomy with reservation of one side as integumentale,the cut line was shape of "匚".Type Ⅱ tumor was with invasion part articular surface,and was operated with part joint osteotomy with reservation of one side as integumentale,the cut line was shape of"L".Type Ⅲ was with invasion total joint surface and as integmentale,all tumor segment including joint could be cut,but the shape of cut close to the diaphysis was echelonment. Result The operation time was 1~3 h,the operative bleeding quantity were 150~400 ml,all incisions were obtained primary healing.Twelve cases were followed up from 6 months to 8 years.The average extremity functional score according to the system of ISOLS(International Society of Limb Salvage):upper extremity was 22.3 and the lower was 25.3 The average bone healing time of returned deactivation bone graft and bone allograft healing with the osteotomy plane was 3 months,original tumor recurrence in local was 1,the recurrence rate was 7.69%,no malignant change and metabasis was found. Conclusion The reservation cortical expand resection and reconstruction could increase the treatment effect of extremities bone tumor,it is key to decrease the recurrence with strict indication,non-neoplasma and aseptic technique.Tumor shell and osteotomy plane should be deactivated thoroughly.

[Objective]To investigate the restoration of the axial alignment of the lower extremity during operation for fracture of tibia plateau and to assess the curative effect.[Method]A retrospective analysis of 45 cases of fracture of tibial plateau were conducted from May 2000 to June 2005,25 male cases and 20 female,mean age were 45.3.The type of fracture:3 cases were Schatzker I,10 were type Ⅰ,19 were type Ⅲ,6 were type Ⅳ,5 were type Ⅴ,2 were type Ⅵ.All cases were treated with open reduction,bone graft and internal-fixation with plate and screw under arthroscopy-assisted,and the normal axial alignment of the lower extremity was restored.The restoration standard was in accordance with the uninjured side limb.The way of measurement:the axial line from anterior superior iliac to interdigit of the first and second toe was done,then,the perpendicular distance from the center of patella to this line was calculated,and compared the data with uninjured side limb's.[Result]Forty cases were followed up,from 6 months to 24 months,average 15 months,according to the Rasmussen knee score standard,24 were excellent,11 were good,3 were fair and 2 were poor.The satisfactory rate was 87.5%,the restoration of knee function were close correlated with whether the line normal or not,there were abnormal lines recurrence in some cases,the main causes were:(1)Meniscus to be cut excessive to the loading redistribution in joint;(2)The reduced articular surface collapsed,again due to the affected limb weight loading too early;(3)Fractures were too complicated to be compress fixed with common buttress plate,resulted in displace fracture.[Conclusion]It is very important that the axial alignment of the lower extremity to be corrected during operation to the injured knee function restoration after operation,and the causes result in the axial line abnormal above-mentioned should be considered thoroughly,at the same time,the corresponding measures should be taken.

[Objective]To explore the treatment of intertrochanteric fracture with expandable proximal femoral nail and prevention and cure of some related complications.[Method]Fifty-eight cases of closed intertrochanteric fracture,including 28 male cases and 30 female,average 73.2 years old(range from 42 to 88)accepted treatment.The type of fracture according to Evans classification:5 cases were type Ⅰ,7 were type Ⅱ,28 were type Ⅲ,13 were type Ⅳ,and 5 were type Ⅴ.All cases were treated with expandable proximal femoral intramedullary nail after close reduction.[Result]Thirty-eight cases were followed up,from 12 to 36 months,average 18.5 months,all of the cases got clinical healing after 8-10 weeks postoperatively.Hip Harris score average was 91.8.Some complications occurred in 6 cases:1 case had secondary fracture nearby the greater trochanter needling insertion point,1 case femoral head peg was outside of the lock hole,and 1 case femoral head peg tip was broken during operation.And after operation,1 case had coxa vara deformation,1 case had femoral middle and superior segment fracture,and 1 case femoral head peg was exited.[Conclusion]Expandable proximal femoral intramedullary nail has some advantages as:minimal invasive,manipulation convenient,fixation reliability,remove easily,but surgeons must be familiar with the construction and fixation principle of the nail in the primary stage,and perform the operating instruction strictly,to reduce or avoid the complications occurrence because of mis-manipulation.