OBJECTIVE:To discuss clinical pharmacists' role in clinical drug treatment. METHODS: Case study was performed retrospectively on clinical pharmacists' involving in clinical drug treatment. RESULTS & CONCLUSION: Hospital pharmacists' participating in drug treatment had achieved remarkable outcome. Clinical pharmacists have to cooperate with medical staff in clinical drug treatment so as to bring their due role into full play.

The purpose of the present study is to establish the physical fitness service network for Shanghai citizens aged 20～69 years,based on the national physical fitness monitoring system and lab testing techniques.The established physical fitness testing network now is ready for use through internet in Shanghai to promote the Shanghai as a "Healthy City".

China ; epidemiology ; Hepatitis, Viral, Human ; epidemiology ; Humans

Objective To establish an equation of oxygen consumption in obese children and adolescents by multilevel linear model analytical method. Methods Sixty obese children and adolescents aged 11-16 years were randomly divided into the formula group(n=40) and back substitution verification group(n=20), with equal male and female. Subjects in formula group started exercise in the speed of 2km/h, increased lKm/h every 2.5 minutes till 8km/h, without gradient in treadmill and 80% HRmax was set as standard of exercise termination. Gaseous metabolism parameter and heart rate were measured for the set up of multiple linear regression equation of oxygen consumption. Back substitution verification was also made. Results Males' R~2 was 0.795 and females' R~2 was 0.75 in regression equation; oxygen consumption had a linear relationship (P0.001) with HR and LBM; The significance of variation coefficient and constants were obvious. The regression equation were set up as male:oxygen consumption(ml/min)=20.098xheart rate(bmp)+25.160×LBM(kg)-2935.835 and female: oxygen consumption(ml/min)=19.863xheart rate(bmp)+26.551×LBM(kg)-2675.676. The predictive value of oxygen consumption of the average relative error was 6.84%～8.72% in male, and 6.53%～8.23% in female. Conclusion The multiple regression equation of oxygen consumption with heart rate and body weight as independent variables might be applied in the calculation of the oxygen consumption of physical activity in obese children and adolescents.

Objective To detect the expressions of CXCR4,Smoothened (Smo) and Patched (Ptch) in breast cancer,to analyze the clinical significance of the expression of CXCR4,and to evaluate the relationships among CXCR4,Smo and Ptch.Methods The expressions of CXCR4,Smo and Ptch from 121 cases of breast cancer specimens were detected by immunohistochemistry,and the results were analyzed.Results The positive expression rate of CXCR4 in breast cancer was 66.9％,which was positively associated with the lymph node metastasis (r =0.181,P =0.044).The positive expression rates of Smo and Ptch in breast cancer were respectively 58.9％ and 64.5％.The expressions of Smo and Ptch were positively associated with the expression of CXCR4 in breast cancer (r =0.189,P =0.036 ; r =0.230,P =0.010).Conclusion The expression of CXCR4 in breast cancer is associated with the lymph node metastasis,and it is positiviely associated with the expressions of Smo and Ptch.Patients with breast cancer who express both CXCR4 and Hedgehog signaling pathway may have a higher risk of recurrence.

Objective To expolore the changes of coagulation index in newborns of different age and its clinical significance. Methods We studied 129 newborn infants without complication admitted to the neonatal department in an all-around hospital in Gnangzhou from January to December in 2005. These neonates were divided into two groups, including 63 cases of full-term infants and 66 cases of premature infants. Prothrombin time (PT), activated partial prothrombin time (APTT), Fibrinogen (Fg), thrombin time (TT), D-dimer (D-D) of these newborn infants were assayed on the first day, the third day and the tenth day. Results In the postnatal three periods of time, there was no difference in PT and D-dimer between two groups(P >0.05), but APTT and TT in premature infants were higher than that in full-term infants( P < 0.05), and Fg in premature infants were lower than that of full-term irfants(P<0.05). PT,APTT, TT,Ddimer were in decrease tendency and Fg increased as age increased( P < 0.05 ). Conclusion Geststional age and age had effect on coagulation index in newborn infants, and both can provide clinical references to clinical diagnosis and therapy.

BACKGROUND:SenseWear Pro Armband (SWA) is a monitoring device of physical activity energy consumption which consists of many kinds of motion sensors, and it has been widely used in the study of physical activity energy consumption in recent years. OBJECTIVE:To summarize the SWA accuracy, software version, time of wear as wel as its application in different populations and at different physical activity levels, for the purpose of providing some practical reference in the future usage of SWA. METHODS:A computer-based search of CNKI (ful-text database), PubMed and Web of Science databases was done for relevant articles published before January 2015 using the keywords of “physical activity, sensewear pro armband, energy expenditure, review” in Chinese and English, respectively. Articles published in core journals or SCI database were preferred. RESULTS AND CONCLUSION:Double labeled water method and indirect measurement method serve as standard criteria, and the relevant study shows that SWA has higher accuracy and can effectively evaluate the daily physical activity energy consumption. But under the special circumstances, there are some differences, such as different people and different exercise intensities. Meanwhile, to determine the appropriate wearing time and software version for data statistics are also one of the factors influencing the accuracy of the final result. SWA can accurately predict physical activity energy consumption, but for different populations and exercise intensities, it is necessary to improve the further calculation formula of SWA.

OBJECTIVE:To prepare Qingyang tea bag and establish the standard of quality control.METHODS:The Flos Lonicera and Radix Glycyrrhize were identified by TLC.The content of chlorogenic acid,the main active component in the tea derived from Flos Lonicera,was determined by TLCs.RESULTS:Linearity was found in the range from1.2?g～4.8?g of chlorogenic acid,r=0.9993;the average recovery was99.50%and RSD=2.39%.CONCLUSION:The method was found to be simple,precise and may be used for the quality control of the preparation.

OBJECTIVE:To promote the computerized management of drug database.METHODS:The function of drug database software development system(SDS)developed by the co-efforts of pharmacy department and information center in our hospital was introduced.RESULTS:With hospital information system,the software can conduct statistics and queries of patients’drug database information at any time.CONCLUSION:The computerized management of drug database can provide prompt and efficient data support for rational drug administration and which is in the benefit of the development of clinical pharmacy work.

Aim To investigate the molecular mechanism of Rosiglitazone on the expression of integrin ?1 in lung pulmonary carcinoma A549 cell line. Methods RT-PCR and flow cytometry methods were used to examine the expression of mRNA and cell total protein of PPAR? and integrin ?1 subunit. Results The expression of PPAR? mRNA and integrin ?1 mRNA had dose-dependent regulating action by Rosiglitazone,using PPAR? inhibitor GW9662 and ERK pathway inhibitor PD98059 could abolish the inhibition of PPAR? on the expression of integrin ?1. Conclusion The effect of PPAR? ligand Rosiglitazone on integrin ?1 expression is mediated through PPAR?-dependent signaling parthway. The mechanism may be partly involved in ERK pathway.