ObjectiveTo explore the effect of various forms of psychological intervention on psychological stress,mental state and treatment process of out- patients. Methods 198 outpatients from February 2010 to July 2011 were randomly divided into the observation group and the control group with 99 cases in each group.The control group received conventional treatment and care.The observation group was given multiple forms of psychological intervention on the basis of conventional treatment and care.Both groups were taken with Zung Anxiety Rating Scale (SAS)and the Hamilton Anxiety Scale(HAMA)to measure the psychological stress and the degree of anxiety before and after treatment.The total efficiencies of various disorders for the two groups before and after the diagnosis and treatment were compared. ResultsThere were no significant differences in SAS score and HAMA scores before psychological intervention.After the intervention,the SAS score and HAMA score in the control group were significantly higher than the observation group.The total effective rate after a course of treatment in the observation group was 90.6％.The total efficiency in the control group was 78.8％,which was significantly lower than that of the observation group. ConclusionsThe multiple forms of psychological intervention can reduce the mental stress,ease the patients' emotional state and improve the patients' therapy effect at the same period for out-patients.

Studies on the variation of amylase, lipase and lrotease activity of Whitmania pigra in 0-6 months old using 3, 5-dinitro- salicylic acid colorimetry, right-nitrophenyl palmitate ester (ρ-NPP) colorimetry and folin-phenol method. The results showed that pro- tease activity remained low before 1.5 months old and with the highest activity in 2 months old, but after showing a small peak in 4 months, alkaline protease rapid declined. Amylase was low at born, then gradually increased the activity of the highest in 2.5 months old. Lipase with a strong vitality at birth, then 1 month with minimum and 2 months peaked, but appeared a small peak in 4 months old. In summary, only lipase exhibits strong activity at birth, lipase with the strongest activity in the digestive tract during develop- ment. Protease, lipase and amylase with the strongest activity at 2-3 months old, but were decreased after 4 months old.
Age Factors ; Amylases ; metabolism ; Animals ; Leeches ; enzymology ; Lipase ; metabolism ; Peptide Hydrolases ; metabolism

Mouthpart developmental histology of Whitmania pigra at different month of age were studied by paraffin section, HE staining combined alcian blue and periodic acid schifts reaction procedure (AB-PAS). The following results was obtained: Change ranges: oral width 0.6 mm (1-3 month), 1.2 mm (34 month); oral diameter 0.3 mm (1-3 month); 1.2 mm (34 month), the oral size reached maximum during 4-6 months and unchanged thereafter. Oral lip had a thin protective film located in the front of the mouthpart. The W. pigra possessed three jaws in oral cavity, the big one was in dorsum, the other two separated on both side of abdomen respectively. Jaws and muscular pharynx were interrelated closely. The jaws were composed by cuticle, epithelial layer, muscularis and jaw cavity from outside to inside. In the front of jaws had mastoid abdomen with function of secreting acidophilic granule from 2 month age. Oral cavity was composed by mucosa, submucosa and muscularis inside and outside. Oral cavity was rich of peristomial nerves. And pharynx was composed of mucosa, muscularis, adventitia from inside to outside. The folds height and width become heighten and thicken. Mucosa epithelium from complex flat epithelium changed into columnar epithelium, muscularis gradually developed into thickened along with growing. Muscular thickness reached maximum at 4 months. Mucous cells of W. pigra were classified into I-IV types based on different staining and two mainly morphological shapes (Tubular, Pear-shaped). Jaws, oral cavity, pharynx by AB-PAS staining showed little changes at different month of age. Mucous cells were few at 1 month age, and type II cells were increased rapidly in 2-3 month age in oral lip. Oral cavity contains more mucous gland cells type I. Under the muscularis there were connective tissues which distributed a few of mucous cells type II.
Animals ; Female ; Histology ; Leeches ; anatomy & histology ; chemistry ; classification ; growth & development ; Male ; Mouth ; anatomy & histology ; chemistry ; Mucous Membrane ; chemistry

OBJECTIVETo study the oxygen consumption rate and suffocation point of Whitmania pigra.

METHODUsing the method of respiratory chamber, the oxygen consumption rate, oxygen consumption volume and suffocation point were obtained.

RESULTThe oxygen consumption rate(OCR) ranged from 0.049 to 0.094 mg x g(-1) x h(-1), the oxygen consumption volume(OCV) from 0.44 to 0.67 mg x p(-1) x h(-1), and suffocation point(SP) from 0.9 to 1.51 mg x L(-1), when the average weight of W. pigra was 10 g and the water temperature varied from 15 to 35 degrees C. OCR ranged from 0.044 to 0.058 mg x g(-1) x h(-1), OCV from 0.19 to 0.77 mg x p(-1) x h(-1) and SP from 1.4 to 1.57 mg x l(-1) when water temperature was 20 degrees C.

CONCLUSIONOCR rose when the water temperature and weight increased, but SP was justly opposite. The weight was negatively correlated with OCR and positively with OCV. OCR had little difference from day to night.

Animals ; Leeches ; metabolism ; physiology ; Oxygen ; metabolism ; Oxygen Consumption ; physiology ; Temperature ; Water

Objective To investigate the inhibitory effect of adriamycin in combination with hyperthermia on apoptosis and bcl-2 expression in the chronic leukemic cell line K562/AO2 in vitro.Methods The working con- centration of adriamycin against K562/AO2 determined by using methyl thiazolyl tetrazolium(MTT) assay was used to treat the chronic leukemic cell line K562/AO2 in vitro alone or in combination with hyperthermia induced using a hot water bath at 40,41 or 42℃.The inhibitory effect was evaluated by MTT assay.The apoptosis rates and bcl-2 ex- pression of K562/AO2 were determined by flow cytometry.Results The working concentration of adriamycin in the study was defined as its 50% inhibition concentration (IC50).A 60 min session of hyperthermia at 40℃,41℃or 42℃was associated with significant growth inhibition of the cell line K562/AO2.Adriamycin chemotherapy alone and with hyperthermia significantly inhibited the growth of K562/AO2.All treatments significantly increased apoptosis rates and down-regulated bcl-2 expression of the K562/AO2 cell line.Conclusion Adriamycin chemotherapy com- bined with 60 min sessions of hyperthermia showed significant suppression effect on K562/AO2 cell proliferation.The treatment can increase apoptosis rates and down-regulate bcl-2 expression.

0.05).2.There were significant differences in the levels of TNF-? after treated for 4 weeks(P0.05).4.There were no significances between the 2 groups before and after treated 6 weeks in the change of liver.Conclusions Matrine injection can inhibit HCMV DNA replication.It can also control the expression of TNF-? and regulate the function of the immune system.There fore matrine injection has an antivirus efficacy in HCMV infection.

OBJECTIVETo analyze the changes of inhibitory killer cell immunoglobulin-like receptors (KIRs), NKG2D receptor and the cytotoxicity of natural killer (NK) cells induced by persistent exposure to CNE2 cells.

METHODSThe HLA-class I genotypes of CNE2 cells and KIR genotypes were determined by PCR with sequence-specific primers (PCR-SSP). The expressions of KIR2DL1, KIR2DL3, KIR3DL1, and NKG2D by the NK cells (freshly isolated NK cells, NK cells cocultured with 100 U/ml IL2 or with 100 U/ml IL2 and CNE2 cells as the control, IL2 and CNE2 groups, respectively) were analyzed by flow cytometry. Cytotoxicity of NK cells against CNE2 cells were detected by LDH releasing assay.

RESULTSThe HLA genotypes of CNE2 cells were A2, 24, B18, 35, Cw4, 7. NK cells isolated from 3 healthy donors expressed KIR2DL1, KIR2DL3, and KIR3DL1. After 4, 24 and 48 h of culture, NK cells in CNE2 group displayed higher KIR2DL1, KIR2DL3 but lower NKG2D expression than those in the control and IL2 groups (P<0.01), whereas the latter two groups showed no significant difference in KIR2DL1, KIR2DL3, and NKG2D expressions (P>0.05), and no difference in KIR3DL1 expression was found between the 3 groups (P>0.05). After 24 h of culture, the cytotoxicity against CNE2 cells mediated by the NK cells in IL2 and CNE2 groups were (26.96-/+1.47) % and (2.74-/+1.64) % at E:T ratios of 10:1, and (35.74-/+3.59)% and (4.57-/+2.41) % at E:T ratio of 20:1, respectively. NK cells in CNE2 group displayed lower cytotoxicity than those in IL2 group (P<0.01).

CONCLUSIONSPersistent exposure to tumor cells expressing NKG2D ligands can lead to downregulated expression of NKG2D receptor, increased expression of KIRs and reduction of NK-mediated cytolysis. These results elucidate the molecular mechanism of reduced cytotoxicity mediated by the edited NK cells and indicate that blocking HLA-class I-bound KIRs or enhancing the expression of NKG2D may promote NK cell-mediated cytolysis.

Cell Line, Tumor ; Cell Survival ; immunology ; Cytotoxicity, Immunologic ; immunology ; Flow Cytometry ; Humans ; Killer Cells, Natural ; cytology ; immunology ; metabolism ; NK Cell Lectin-Like Receptor Subfamily K ; Nasopharyngeal Neoplasms ; immunology ; metabolism ; pathology ; Receptors, Immunologic ; metabolism ; Receptors, KIR ; metabolism ; Receptors, KIR2DL1 ; metabolism ; Receptors, KIR2DL3 ; metabolism ; Receptors, Natural Killer Cell

OBJECTIVETo determine the best spawning temperature and weight of Whitmania pigra and the hatching temperature of its eggs.

METHODObserve the state of W. pigra spawning and hatching were observed under different temperatures and weights conditions.

RESULT AND CONCLUSION25 degrees C is the most appropriate temperature for W. pigra spawning and incubation, the best spawning weight of W. pigra was around 20 g. The temperature influenced the lost weight after spawning significantly and was proportion to the lost weight. The body weight showed no substantial influence to the lost weight after spawning. The egg weight of W. pigra which body weight was below 2.0 g was proportion to the hatching rate. The same relationship was not observed in W. pigra over 2.0 g.

Animals ; Body Weight ; Breeding ; methods ; Female ; Leeches ; growth & development ; physiology ; Male ; Ovum ; growth & development ; Temperature

OBJECTIVETo observe Whitmania pigra growth and intake law in different temperature conditions and patterns.

METHODComparison of W. pigra growth in different weight and different temperature conditions, and observe the intake law of W. pigra within 24 h.

RESULT AND CONCLUSIONThe appropriate temperature for W. pigra growth is 15 - 25 degrees C. Its largest weight of intake increas with the weight of W. pigra and temperature rise, but with the intake rate increase, individual intake reduced. W. pigra intake have the two peaks in 24 h.

Animals ; Circadian Rhythm ; Feeding Behavior ; physiology ; Leeches ; growth & development ; physiology ; Temperature

OBJECTIVEThrough observation of the tissue structures of internal organs and skin of Whitmania pigra, to provide basic method for identification of W. pigra and research of its physiological functions.

METHODThis experiment adopted the W. pigras as the material and carried on dissection observing,and W. pigra's cavity, swallow, esophagus, crop, bowel, rectum, parasomal sac stomach, testicle, seminal vesicle, vasa deferentia duct, ejaculatory ball, prostate gland and skin was observed by the method of the paraffin organization slice and microphotography.

RESULT AND CONCLUSIONThe structures and functions of the skin and the organ of the digestive and reproductive system, as well as the types and distributions of the mucous cells were acquired,which provided abasis for further study on the living behaviour, propagating behaviour and breeding environment of W. pigra.

Animals ; Digestive System ; anatomy & histology ; Genitalia ; anatomy & histology ; physiology ; Leeches ; anatomy & histology ; physiology ; Skin ; anatomy & histology ; Skin Physiological Phenomena