BACKGROUND:When leucocytes were activated in the process of lung ischemia-reperfusion,various cell factors will be released,including tumor necrosis factor ?(TNF-?) and interleukin 1?(IL-1?),which lead to ischemia-reperfusion injury.Recent research demonstrates that transforming growth factor-?1 plays a protective role in ischemia-reperfusion injury after lung transplantation OBJECTIVE:To investigate the level changes of TNF-? and IL-1?after interfered with transforming growth factor-?1 after lung ischemic-reperfusion injury DESIGN,TIME AND SETTING:The randomized controlled experiment of animal was performed in the Cardiothoracic Surgery Laboratory of Changhai Hospital,Second Military Medical University of Chinese PLA from August to October 2007.MATERIALS:Thirty Sprague-Dawley rats with clean grade,were randomly divided into the sham operation,ischemia-reperfusion and transforming growth factor?1 groups,with 10 animal in each group.The transforming growth factor?1 was phuchased from Peprotech INC.METHODS:The left lung of rat in the ischemia-reperfusion and transforming growth factor?1 groups was prepared for to ischemia-reperfusion model by blocking pulmonary circulation with hilar vessels ligation for 60 minutes,followed by reperfusion for 2 hours.Animals in the sham operation group were crossed hilar with 10# silk thread.Heparin was administered intravenously through vena cervicalis at 15 minutes before ischemia in each group.Concurrently,10 ?g/kg transforming growth factor?1 was administered in transforming growth factor?1 group.MAIN OUTCOME MEASURES:After 2 hours of ischemia-reperfusion,the tissues of left lung were removed,histomorphological changes,wet-dry weight ratio,the index of histological lung injury and the content of TNF-?and IL-1?were determined by light microscope,enzyme-linked immunosorbent assay(ELISA),respectively.RESULTS:A total of 30 animals were enrolled in the result analysis.There was no obvious pathological change of lung in the sham operation group.Visible tissue edema and neutrophil infiltration could be found in the ischemia-reperfusion group,while,there was mild tissue edema and little neutrophil infiltration in the transforming growth factor?1 group.The wet-dry weight ratio and index of histological lung injury in the ischemia-reperfusion group was obvious greater than that of the sham operation group(P

Adult ; Electrolysis ; Humans ; Lipid Peroxidation ; drug effects ; Male ; Middle Aged ; Nickel ; adverse effects ; blood ; pharmacokinetics ; Occupational Exposure ; adverse effects

Animals ; Arterioles ; cytology ; growth & development ; Cell Culture Techniques ; methods ; Cell Proliferation ; Cells, Cultured ; Lung ; anatomy & histology ; Myocytes, Smooth Muscle ; cytology ; physiology ; Rats ; Rats, Sprague-Dawley

Objective To discuss the consequences of two different methods of high pressure injection in CT contrast enhancement to display the aortic dissecting aneurysm.Methods 100 patients underwent Lightspeed 16 MS CT with contrast enhancement of Stellant D high pressure injector(Medrad),injecting speed of 4.0 ml/s and 80 ml dosage of contrast medium.All patients were divided into A and B groups with 50 in each.The single high pressure injection was applied to A group without isotonic Na chloride flush.B group underwent the same high pressure injection and followed by isotonic Na chloride flush.The method of evaluation was carried out by double blind observation.Results A group revealed radiologic artifact up to 40 cases with positive rate of 80%.B group demonstrated the same kind of radiologic artifact in 26 cases with positive rate of 52%.Conclusions Using normal saline(sodium chloride solution)flush after high pressure injection of contrast medium during MSCT angiography is obviously better to demonstrate the internal structures of treatvessels.

Objective To observe the effect of blood-cooling and blood-stasis-removing Decoction on anti-double-strain DNA ( anti-dsDNA) antibody, anti-nucleosome antibody ( AnuA) and serum levels of interleukin ( IL) -6, 17, 21 in spontaneous systemic lupus erythematosus ( SLE) MRL/lpr experimental rats. Methods The experimental rats were divided into blank control group, model group, and high- and low- dose Chinese medicine groups ( 25.2, 12.6 g/kg respectively) , the treatment lasting 8 weeks. At the end of the experiment, the blood taken from the orbital veins was separated for obtaining serum, and then the serum anti-dsDNA antibody, AnuA, IL-6,17,21 levels were tested by enzyme-linked immunosorbent assay ( ELISA). Results The serum autoantibody and IL-6, 17, 21 levels of rats in the model group were increased significantly as compared with the blank control group ( P<0.01). The serum antibody and cytokine levels of Chinese medicine groups were reduced as compared with the model group, the difference between high-dose Chinese medicine group and model group being significant ( P<0.01). Conclusion Blood-cooling and blood-stasis-removing Decoction has certain effect on reducing the serum levels of anti-dsDNA antibody, AnuA, and IL-6,17,21, which may coniribute to one of its therapeutic mechanisms for SLE.

Objective To discuss the application of cervical lateral mass plate for the treatment of fractures and dislocations of the cervical spine. Methods From January 1998 to January 2001, internal fixation with cervical lateral mass plate and bony fusion were performed in 21 patients during posterior decompression for the treatment of fractures and dislocations of the lower cervical spine . A posterior median approach was used to expose the lateral edge of the articular process. All patients received spinal decompression and reduction according to the types of fracture and dislocation. A cervical lateral mass plate was applied in each lateral mass, a suitable bicortical screw was inserted using Magerl technique (the inserting point of the screw was 2 mm medial and superior to the center of lateral mass, the direction was 20?-25? laterally and 30?-40? cranially). Results The follow-up of all 21 cases ranged from 9 months to 3 years and 9 months (with the mean of 27 months). There were 20 cases with complete reduction of antero-posterior and rotational dislocation, and 1 case with incomplete reduction according to post-operative X-ray film. Patients could sit up with the protection by wearing soft collar 4 days (2-7 days) after the surgery. The mean off-bed time of those without spinal cord injuries or with spinal cord injuries less than Frankel C grade were 9 days (3-14 days) after operation. All cases obtained solid bony fusion 6 months postoperatively. Eighteen cases with spinal cord injury improved one ASIA grade, 2 cases without root injury obtained complete recovery eventually. No severe complications such as injuries of vertebral artery, nerve root and spinal cord or aggravation of spinal cord injury occurred. Conclusion Cervical lateral mass plate fixation has the advantages of wide indications, strong stability, short segment fixation and easily reduction. The results suggested that this technique is an efficient, reliable segmental posterior fixation.

Objective To review the clinical results of percutaneous laser disc decompression(PLDD)in the treatment of cervical disc herniation.Methods PLDD was adopted in the treatment of cer-vi cal disc herniation in32patients.There were19males and13females with an average age of 54years,ranging from33to67years.The lesions were located at the levels of C 3,4 in4cases,C 4,5 in11cases,C 5,6 in13cases and C 6,7 in7cases.The laser fiber was introduced into the herniated disc space with the needle puncturing percu taneously in anterior surface of neck under fluoroscopic guidance.The ND:YAG laser was of 1060nm wave-length,and the diameter of the fiber was400?m.The laser output power was15w with1s emission and5s interval.The compression of spinal cord and surrounding tissue was eliminated by reducing the intra-disc pressure through the vaporization of the disc nucleus.The total laser output power was decid ed depending on the age of the patients,degenerative degree of the disc and the reactive process of heat.The total powers of the group were360J to1100J.Results The mean follow-up was8months,ranging from3to15months.The clinical evaluations were classified as excellent in16cases(50%),good8cases(25%),fair4cases(12.5%)and poor 4cases(12.5%).The general effective ratio was87.5%,the ex cellent and good ratio was75%.The remission ratio of the symptoms was80.7%for the neck and shoulder pain,84.2%for ra-diating pain of the nerve roots of upper extremities,60%for symptoms of the lower ex tremities and78.5%for cervi cal dizziness.No complications occured.Conclusion PLDD could relieve the symptoms and signs of patients cervical disc herniaton.PLDD is easy to manipulate,safe,mini-invasive,and the patients recov-ered sooner with less complications.

[Objective]On May 12,2008,an over 8.0-magnitude earthquake occurred in Wenchuan county,Sichuan province,China.The anthor's field hospital was deployed to An county,which arrived on the 3rd day after the disaster.During the first week of the deployment,284 victims with musculoskeletal injuries received orthopedic management.The objective of this article is to highlight the experience gained by this author's medical assistance team,focusing on the orthopedic care in the early part of the post-disaster relief activities.[Method]Records of 284 patients who underwent orthopedic management were reviewed retrospectively.[Result]There were a large number of musculoskeletal injuries amongst the survivors in the acute phase of the disaster,including spinal trauma,fractures and dislocations,peripheral nerve injuries and soft tissue injuries.The average age was 49.3 years(range,11 to 92 years) with 133 men and 151 women.The patients with upper extremity fracture were 85,with lower extremity fracture 73,with spinal fracture 23,with shoulder or hip dislocation 7.The patients with hand or foot injuries,peripheral nerve injuries and soft tissue injuries were 96.Open injuries were 67,the remaining were closed injuries.Totally 131 patients recovered,and the rest were transferred to the rear hospital for further management.The X-ray postoperatively showed that 21 open fractures were reduced functionally by means of external fixator,and the shoulder or hip dislocations were reduced anatomically.The wounds with primary suture had no infection.There were no other complications.[Conclusion]Correct diagnsis of the injuries,providing scientific and resonable therapeutic measures,and correct operative indication should be emphasized for early orllopedic care.Definitive surgeries such as open reduction and internal fixation are not recommended strongly.External fixation for post-earthquake open fractures is simple and effective,allowing simple approach to wound care and transferring to rear hospital during the whole period of fixation.

Objective To explore the influence of the sequential enteral nutrition(SEN) on nutritional status and prognosis in patients with acute cerebral infarction(ACI).Methods 80 ACI patients complicated with dysphagia were divided into SEN group and control group(40 cases in each group).In the first three days patients in SEN group were treated with nasal feeding short-peptide enteral nutrition[84~126(kJ/kg.d) ],than,which would be replaced with nasal feeding integral-protein enteral nutrition from the forth day.Patients in the control group were provided with ordinary fluid food by nasal feeding.Nutrition support for both groups was at last more than 10 d.In the 1 d,7 d and 14 d after treatment,data of both groups concerned with triceps skin fold(TSF),mean upper arm muscle circumference of triceps(MAMC),serum albumen(Alb),serum prealbum(PAB),heamoglobin(Hb),incidence of infectious complications,National Institutes of Health Stroke Scale(NIHSS) score and Barthel index(BI) were examined.Results Nutritional indexes(TSF,MAMC,Alb,PAB,Hb) in the SEN group were significantly higher than those in the control group(P

Objective To investigate the relationship between Oxfordshire community stroke project(OCSP) classification and imaging classification in acute cerebral infarction. Methods Two hundred and thirty-six patients with acute cerebral infarction were retrospectively evaluated with OCSP classification and imaging characteristics. Results According to OCSP classification,of all the 236 patients with acute cerebral infarction,28(11.9%) experienced total anterior circulation infarction(TACI),71(30.1%) partial anterior circulation infarction(PACI),94(39.8%) lacunar infarction(LACI),and 43(18.2%) posterior circulation infarction(POCI).The consistency was found in 171 cases(72.5%) between the OCSP classification and imaging classification,with the accuracy of 76%(25/33) for TACI,81%(34/42) for PACI,71%(81/114) for LACI and 66%(31/47) for POCI. Conclusion OCSP classification can predict the location and size of cerebral infarction with a high accuracy,and is well consisted with the imaging findings.