Objective To study the genotype distribution and its association with acute coronary syndrome (ACS) of polymorphism of osteoprotegerin (OPG) gene promoter rs2073617T/C(950T/C) and the first exon rs2073618G/C(1181G/C) in Fujian Han population. Methods A total of seven hundred and twenty consecutive patients of unrelated Fujian Han people with ACS were enrolled in the present study. They were divided into ACS group and control group, 360 each. OPG gene 950T/C and 1181G/C were genotyped in all the patients using polymerase chain reaction restriction fragment length polymorphism (PCRRFLP). DNA sequences of enzyme digestion products were also analyzed. Results In Fujian Han population, the frequency of TC, TT, CC types of OPG 950T/C polymorphism in ACS patients and controls were 47.8%, 26.7% and 25.5% vs 43.3%, 33.3% and 23.3%; The frequency of GG, GC, CC types of OPG 1181G/C polymorphism in ACS patients and controls were 51.1%, 40.0% and 8.9% vs 60.0%, 35.0% and 5.0%. No significant differences were observed in the genotype and allelic frequency of OPG gene 950T/C and 1181G/C between the ACS and control groups. OPG gene 950T/C and 1181G/C showed no statistically significant difference between ACS patients with single-vessel, double-vessel and three or more-vessel disease. Conclusion OPG gene polymorphism of 950T/C and 1181G/C are not associated with ACS in Han population of Fujian.

Objective To observe the hemodynamic changes during operation of piggyback liver transplantation.Methods The Swan-Ganz catheter was inserted and the parameters of hemodynamics were analyzes in 36 patients with chronic hepatic diseases following piggyback liver transplantation. The cardiac output(CO)and pulmonary arterial pressure(PAP)were measured.Results CO and PAP were decreased significantly in the anhepatic phase(P

Objective To study the genotype distribution and its association with acute coronary syndrome (ACS) of polymorphism of osteoprotegerin (OPG) gene promoter rs2073617T/C(950T/C) and the first exon rs2073618G/C(1181G/C) in Fujian Han population. Methods A total of seven hundred and twenty consecutive patients of unrelated Fujian Han people with ACS were enrolled in the present study. They were divided into ACS group and control group, 360 each. OPG gene 950T/C and 1181G/C were genotyped in all the patients using polymerase chain reaction restriction fragment length polymorphism (PCRRFLP). DNA sequences of enzyme digestion products were also analyzed. Results In Fujian Han population, the frequency of TC, TT, CC types of OPG 950T/C polymorphism in ACS patients and controls were 47.8%, 26.7% and 25.5% vs 43.3%, 33.3% and 23.3%; The frequency of GG, GC, CC types of OPG 1181G/C polymorphism in ACS patients and controls were 51.1%, 40.0% and 8.9% vs 60.0%, 35.0% and 5.0%. No significant differences were observed in the genotype and allelic frequency of OPG gene 950T/C and 1181G/C between the ACS and control groups. OPG gene 950T/C and 1181G/C showed no statistically significant difference between ACS patients with single-vessel, double-vessel and three or more-vessel disease. Conclusion OPG gene polymorphism of 950T/C and 1181G/C are not associated with ACS in Han population of Fujian.

This study was aimed to compare the effects of acupuncture stimulation on different acupoints, which included LR14-Qimen, ST25-Tianshu, GB34-Yanglingquanand CO11-pancreas and gallbladder (auricular vagus nerve intensively distributed areas) on gallbladder pressure and Oddi sphincter function of guinea pig, in order to discuss acupuncture effect on different acupoints in the regulation of motor function of extrahepatic biliary system. A total of 20 adult guinea pigs of both sexes were randomly divided into two groups, with 10 in each group. The detection was made on changes of Oddi sphincter electromyography and gallbladder pressure before and after acupuncture. A double-hook electrode was implanted into the Oddi sphincter for the detection of Oddi sphincter electromyography. And one small artificial balloon was put into the gallbladder to measure the intrabiliary pressure. After the guinea pig was anesthetized, changes of gallbladder pressure as well as the electromyography frequency and amplitude index of Oddi sphincter of the normal guinea pig were recorded. When the guinea pig was stabilized, acupuncture was given on LR14, ST25, GB34 and CO11 for 1 min. Observations were made on acupuncture effects of gallbladder pressure and Oddi sphincter function of guinea pig. The results showed that after acupuncture on LR14 and ST25, the electromyography frequency of Oddi sphincter was obviously increased compared with before acupuncture (frequency:P < 0.01, amplitude:P < 0.05); the gallbladder pressure was obviously decreased (P < 0.05). After acupuncture on CO11 and GB34, the electromyography frequency and amplitude index of Oddi sphincter were obviously decreased compared with before acupuncture (P < 0.05); the gallbladder pressure was obviously increased (P < 0.01). It was concluded that acupuncture on LR14 and ST25 can obviously inhibit the movement of gallbladder and promote the myoelectricity release of Oddi sphincter. Acupuncturing on CO11 and GB34 can apparently promote the shrinking movement of gallbladder and inhibit the myoelectricity release of Oddi sphincter.

The physiological mechanism underlying the acupoint sensitization was evaluated systemically by using the method of electric physiology at spinal cord, medulla, and thalamus levels; the dynamic change of acupoint from the relative "silence" to the relative "activation" function was explained through the study on the dynamic process of acupoint sensitization; the biological process of the therapeutic effect of acupoint stimulation was illuminated through the research of the central mechanism underlining the dose effect relationship between the sensitive acupoint and the related brain area, thus scientific evidence for the functional link between the acupoint and internal organs as well as the nature of the acupoint were provided.
Acupuncture Points ; Acupuncture Therapy ; Animals ; Humans ; Moxibustion ; Nociceptors ; physiology ; Sensation ; Viscera ; innervation ; physiology ; Visceral Afferents ; physiology

Objective Based on the principles of laser radiation protection, medical metroiogy and photoelectron technology, an automatic verification device and testing technology to provide verification and performance evaluation for laser protective spectacles and equipments which have the various functions in laser protection have been developed and established. Methods The current system comprises laser source, laser measuring instruments, software of computer detection and control and modules of optics and mechanics used in auxiliary equipment. By use of the verification device and the special test-recording method to be designed and studied by us, the measured data can be automatically acquired, processed, recorded, saved and printed and, furthermore, many parameters on protective performance of the measured laser protective spectacles can be tested and given. Results Laser wavelength of the verification apparatus are 1 064 nm and 532 nm, response range of wavelength is from 0.4 m to 1.1 μm, measuring range of laser energy is from 10-8 J to 0.3 J, measuring range of optical density for laser protective spectacles is from 0.1 to 8.0, stability is 0.21%, measuring uncertainty is 5%(k=2). Conclusion The automatic verification device is steady and reliable. The achieved performance indexes accord with the requirement of national standard on laser protection.

Objective To investigate the relationship between the single nucleotide polymorphism in exon 7(G894T) or the insert/deletion polymorphism of variable number of tandem repeat in intron 4(4b/a) of the endothelial constitutive nitric oxide synthase(ecNOS) gene and the coronary disease in patients with type 2 diabetes.Methods In 80 patients with type 2 diabetes(group DM),92 type 2 diabetic patients with coronary disease(group DH) and 119 healthy controls(group NC),the polymorphism G894T was determined by polymerase chain reaction-restriction fragment length polymorphism analysis, while the polymorphism 4b/a was determined by polymerase chain reaction-variable number of tandem repeat analysis.Results The frequencies of allele T or genotype containing with allele T(GT or TT) in group DH were significantly higher than those in group DM or group NC(all P

Objective This study was designed to validate the utility of a population pharmacokinetic model established for vancomycin in patients with severe neurosurgical disease . Methods The clinical data including patient gender , age , body weight ,serum creatinine and albumin were collected retrospectively from patients in Nanjing Drum Tower Hospital to calculate the steady trough concentration of vancomycin using the previously established pharmacokinetic model .The predicted value was compared with the actual value .Results During the period from March 2013 to March 2014 ,53 blood samples with serum trough concentration of vancomycin were collected from 42 patients .The average trough concentration of vancomycin was 10 .9 mg/L (range from 1 .6 to 49 .1 mg/L) .The predicted trough level of vancomycin based on the population pharmacokinetic model was significantly correlated to the actual value(r=0 .857 ,P<0 .001) .The mean absolute percentage error was 0 .407 9 . The confidence interval was 9 .36‐14 .07 for the predicted values ,and 8 .92‐14 .32 for the actual values .Conclusions The pharmacokinetic model is valid and useful for planning intravenous dose of vancomycin in patients with severe neurosurgical disease .Large error (about 30% ) was observed in estimation of body weight due to coma .Reduced renal function following contrast agent and/or diuretic drug has an impact on the predicted results . The accuracy of prediction can be increased to nearly 70% after adjusting the covariates .

Objective To investigate the feasibility of macrophages as cell carriers to deliver floate modified oxygen loaded ultrasound contrast agent . Methods The phagocytic activity of macrophages was analyzed by ink phagocytose test , and the expression of folate recepters ( FRs ) on macrophages cell membrane surface was tested by immunofluofluorescence assay . Oxygen/paclitaxel loaded lipid microbubbles( OPLMB) and folate‐targeted OPLMB ( TOPLMB) were synthesized by mechanical shock method and incubated with macrophages in vitro . According to different treatment conditions ,the cells were divided into three groups:group A ( OPLMB) ,group B ( free folic acid + TOPLMB) and group C ( TOPLMB) , the fluorescence intensity of the cells were observed under fluorescence microscope ,and the phagocytic percentage and the phagocytic index of macrophages uptake OPLMB and TOPLMB were observed by bright field microscope . Results The phagocytic percentage of macrophages phagocytose ink was (99 .3 ± 1 .0)% ,FRs was highly expressed on macrophages cultured in vitro . After incubation for 30 minutes ,the fluorescence intensity of group C was significantly higher than those of A and B ,the phagocytic percentage in three groups were (19 .5 ± 0 .2)% ,(21 .0 ± 0 .2)% and (81 .2 ± 10 .0)% respetively . The phagocytic percentage of group C were significantly higher than those in group A and group B ( P <0 .05) . Conclusions Macrophages cultured in vitro possess highly phagocytic activity and these cells highly express FRs ,and can be used as cell carriers to deliver floate modified oxygen loaded multimodality ultrasound contrast agent .

Adult ; Female ; Hip Dislocation ; physiopathology ; therapy ; Hip Joint ; physiopathology ; Humans ; Male ; Middle Aged ; Musculoskeletal Manipulations ; Treatment Outcome ; Young Adult