Objective To explore the clinical effect of laparoscopy combined with deep hyperthermia in the treatment of pelvic abscess.Methods Clinical data of 56 patients with pelvic abscess treated by laparoscopy combined with deep hyperthermia were retrospectively analyzed.Results 56 cases were completed in laparoscopic surgery,before surgery,after diagnostic accordance rate was 76.8%,average operation time was 51 minutes,postoperative average hospitalization was 5.1 days.After operation they were performed in vitro hyperthermia to increase the efficacy. Patients were followed up for 6 months,no recurrence.32 patients with fertility requirements of the fallopian tube,1 to 3 months after the operation of bilateral fallopian tube radiography,20 cases of patency,8 cases of tubal patency and poor.Conclusion Laparoscopy combined with deep hyperthermia in vitro is an ideal and safe treatment,which is conducive to protect the patients'reproductive function,especially for young patients with no pregnancy.

The Logminer utility has been provided by the latest Oracle 8i version.It can analyze archived log files and online log files.This paper introduces how to install and use this utility,especially how to make the use of the auditing function on key users and key data tables,as well as recover the hostility operation without the shutdown of database.

Objective To explore the effects of exercise with psychological rehabilitation on motor function of upper limbs for stroke patients with hemiplegia. Methods 34 stroke patients with hemiplegia were divided into treatment group (n=17) and control group (n=17). Both groups were treated with basic medication and exercise while the treatment group received psychological rehabilitation additionally. The motor function of upper limbs was evaluated with Fugl-Meyer assessment (FMA), and activities of daily living was assessed with Barthel index (BI) before and after treatment. Results After treatment, the scores of FMA and BI improved significantly in both groups (P<0.001), and was better in the treatment group than in the control group (P<0.001). Conclusion Exercise with psychological rehabilitation can effectively improve the motor function of upper limbs and activities of daily living for stroke patients with hemiplegia.

Objective To investigate the feasibility and effect of recanalization of inferior vena cava with percutaneous transluminal angioplasty(PTA)by Inoue-balloon. Methods Eighty-nine patients with Budd-chiari syndrome(BCS)were treated with PTA by Inoue-balloon. Results After PTA, the median(interquartile range)diameter of hepatic segment inferior vena cava increased from 0.00(0.20 ～ 0.00) cm to 1.90(2.00 ～ 1.47)cm; (P

Objective To identify the pathogenic microorganism by MALDI-TOF MS.Methods A total of 560 strains were resuscitated,which included 260 gram-positive bacteria strains,180 gram-negative bacteria strains,60 yeast-like-fungi strains and 60 enteropathogenic bacteria strains.Comparing MALDI-TOF MS with Vitek2 Compact,the discordant results were validated by 16S rDNA sequencing.Results Comparing MALDI-TOF MS with Vitek2 Compact,the coincidence rate was 94.6％ (246/260) for gram-positive bacteria,96.7％ (174/180) for gram-negative bacteria,95％ (57/60) for yeast-like-fungi,and 93.3％(56/60) for enteropathogenic bacteria Fifteen strains were validated by 16S rDNA gene sequencing.Comparing with sequencing,the coincidence rate of two methods was 66.7％ ( 10/15 ) for MALDI-TOF MS and 26.7％(4/11 ) for Vitek2 Compact,respectively.Conclusion MALDI-TOF MS shows rapid turnaround time and modest reagent costs,and it will be another effective tool for microorganism diagnosis.

Objective To establish an accurate method for determining the content of three components in Fufang Buwu Syrup. Methods TLC scanner was selected to detect three components with silica gel G thin layer plate. The sample was separated by using cyclohexane-ethyl acetate-methylenechloride-formic acid (3∶1∶1∶0.2),λS=300 nm. Results The linearity between peak area and ferulic acid was achieved in the range of 0.36-0.84μg, psoralen was achieved in the range of 0.12-0.28 μg, emodin was achieved in the range of 0.01-0.05 μg. The average recovery was 100.7%, 100.8%, 101.0%, and RSD was 1.26%, 1.44%, 1.86%, respectively. Conclusion The method is simple and accurate, which can be used for quality control of Fufang Buwu Syrup.

Objective To investigate the effects of different lighting(sulfur lamp, heat radiation lamp, fluorescent lamp and LED lamp) on reproductive system in depressive male rats.Methods CUMS depressive model of rats was established through the stimulations of chronic unpredictable mild stress(CUMS), then they were randomly divided into 5 groups:depressive model group, sulfur lamp group, heat radiation lamp group, fluorescent lamp group and LED lamp group.After 45 d of continuous illumination, serum was collected and reproductive organs were removed from rats, then the organ index of testicle,epididymis,seminal vesicle and kidney was calculated, the concentrations of testosterone (T), estradiol (E2), prolactin (PRL) and progesterone (PROG)in the serum were detected by ELISA, the histomorphological lesion of testicle was observed under microscope with hematoxylin-eosin (HE) staining, the expression of melatonin receptors (Mel 1a), 3β-HSD and P450scc of testicle were detected by Western blot.Results The organ index of testicle, epididymis and seminal vesicle, the concentration of T, E2,PRLand PROG, and the expression of Mel 1a, 3β-HSD and P450scc in the depressive rats were significantly lower than that of the controls(P<0.05), and the seminiferous tubules of the testis of depressive rats were atrophied.However, the level of T, E2, PRL and PROG were increased,the cell morphology of the testicle was improved, and the expression of Mel 1a, 3β-HSD and and P450scc were upregulated after the sulfur lamp lighting in the depressive male rats compared with depressive model group (P<0.05).Conclusions The testis structure and functions of depressive male rats are improved by sulfur lamp lighting.

Objective To gather laboratory results automatically.Methods An interface software was developed with Mscomm32 control and PowerBuilder.Result The results of laboratory instruments were input into LIS database automatically.Conclusion Hospital engineer can realize the connection of laboratory instruments with Mscomm32 control and PowerBuilder.

Objective To explore the clinical efficacy and side effect of hepatocyte growth - promoting factor in treatment of infant hepatitis syndrome. Methods Sixty one cases of infant hepatitis syndrome were chased as the treatment group who hospitalized in our hospital from March 2002 to Feb 2003,and 54 cases of infant hepatitis syndrome as control group who hospitalized during March 2001 to Feb 2002. The treatment group were administrated with hepatocyte growth - promoting factor for 2 weeks. We obser-ved the recovery of patient's liver function (TBIL.ALT, AST) and the side effect of hepatocyte growth- promoting factor after two weeks of the treatment. Results After the treatment,TBIL and ALT decreased significantly in the treatment group of infant hepatitis syndrome. The treatment group was superior to the control group (P

Objective To explore the relationship between tumor necrosis factor(TNF)-? promoter G238A,G308A and interferon(IFN)-?+874A/T polymorphism and susceptibility to cytomegalovirus(CMV) hepatitis.Methods A TaqMan fluorescence polymerase chain reaction(PCR) in the IFN-?+874A/T and TNF-? gene promoter region single nucleotide polymorphism was tested in the subjects,including 87 infants with CMV hepatitis and 89 children without CMV hepatitis.Results No evident difference of TNF-? +238G/A and+308G/A allele frequency was found between infants with CMV hepatitis and controls.The cases of IFN-?+874 AA,AT and TT genotype were 64,20,3 cases in the infant CMV hepatitis group,and 45,26 and 18 cases in the control group,respectively.There was significant difference in IFN-?+874 allele genotype and frequency between infants with CMV hepatitis and the control group(P=0.001,P