Objective To identify the predictive factors associated with hepatitis B surface antigen (HBsAg) loss in hepatitis B e antigen (HBeAg)-positive chronic hepatitis B (CHB) patients treated with pegylated interferon (PEG-IFNα-2a).Methods Seventy-two HBeAg positive CHB patients were treated with PEG-IFNa-2a 180 μg weekly for 48 weeks. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and hepatitis B virus (HBV) DNA,HBeAg, and HBsAg were quantitatively detected every 3 months. The relationship between HBV DNA, HBeAg, and HBsAg levels at baseline, week 12, 24 of treatment and HBsAg loss was analyzed.The data were statistically assessed by Fisher's exact test,and receiver operating characteristic (ROC) curve. ResultsTotally 65 patients accomplished the therapy, and 7 (10.8％)patients achieved HBsAg loss. HBsAg loss at week 48 of treatment was associated with HBeAg level at week 12 of treatment (Fisher's exact test, P= 0. 023), HBeAg level at week 24 (Fisher's exact test, P=0. 004), and lower HBsAg levels (＜250 IU/mL) at week 12 and 24 of treatment (Fisher's exact test,P=0. 001 and 0.002, respectively). HBsAg loss was associated with HBV DNA negative ( ＜ 1000 copy/mL) at week 12 of treatment (Fisher's exact test, P = 0. 039), while not associated with HBV DNA negative at week 24 of treatment (Fisher's exact test, P=0. 130). ROC curve analysis revealed that the AUC was 0. 8584(P=0. 0021) of HBsAg level at week 12, 0. 9606(P=0. 001) of HBsAg level at week 24, and 0. 8350(P=0. 040) of HBeAg level at week 24. ConclusionLevels of HBsAg and HBeAg at week 24 of treatment might serve as effective factors to predict HBsAg loss in patients received PEG-IFN monotherapy.

Objective To achieve millisecond grade precision in the design model of event-related fMRI experiment.Methods The impact factors of time precision were analyzed,and solutions were put forward.Results The parameter of E-Prime(a software dedicated to psychology study) was adjusted to get millisecond precision.Conclusion General computer is very difficult to achieve millisecond grade due to software and hardware factors,but it can be achieved through adjusting the parameter of E-Prime.

Objective To investigate the relationship of uteroglobin gene polymorphism to the sus-ceptibility to, clinical type and pathological type of Henoch-Schsnlein purpura (HSP) and Henoch-Schonlein purpura nephritis (HSPN). Methods Totally, 118 patients with clinically diagnosed HSP, including 80 cases of HSPN and 38 cases without renal involvement were recruited in this study together with 100 normal human healthy controls. Genomic DNA was isolated from peripheral blood leucocytes of all subjects. The uteroglobin G38A polymorphism was determined by PCR-restriction fragment length polymorphism (RFLP). Results The frequencies of genotypes 38GG, 38GA and 38AA in normal human controls did not differ from those in patients with HSP, patients with HSP but without nephritis, patients with HSPN, patients with HSP and joint involvement, patients with HSP and gastrointestinal involvement (all P > 0.05). Also, no sig-nificant difference was observed between patients with HSPN and patients with HSP but without nephritis (P > 0.05). Furthermore, the frequency of genotypes 38GG, 38GA and 38AA had no significant correlation to the clinical phenotype of HSP, the occurrence of gross hematuria and nephrotie syndrome or the degree of renal damage (all P > 0.05). A significant increase was observed in the frequency of genotype 38AA in patients with HSP with elevated serum IgE compared with those with normal serum lgE (58.82% vs 8.43%, χ2 = 21.946, P < 0.05, OR = 15.51, 95% CI range: 4.93% - 48.84%), whereas the frequency of genotype 38GG was significantly increased in patients with HSPN and hypertension than in those with HSPN but without hypertension (75.68% vs 18.60%, χ2 = 26.172, P < 0.05, OR = 13.61, 95% CI range: 5.01% -37.01%). Conclusions The uteroglobin G38A polymorphism seems unrelated to the susceptibility to and degree of renal damage in patients with HSP and HSPN. The genotype 38AA may be associated with elevated level of serum IgE In patients with HSP, while genotype 38GG is associated with a high incidence of hyper-tension in patients with HSPN.

Corticotropin-releasing factor(CRF)is one neuroendocrine peptide which is closely related with the stress.It has been proved that any stress can lead to the person and animal's blood CRF level increasing,and high-level CRF urges the hypothalamic neuron calcium ion inflow.Newborn pediatricians pay more attention to whether blood CRF level can act as one director of assessing severity of newborn hypoxic-schemic brain damage.This article reviews the CRF and it's acceptors,CRF secretion,CRF physiological action and it's adjustment,and hypoxia-ischemia stress and CRF,in order to provide the theory basis for reviewing the severity of hypoxic-schemic brain damage in neonates.

Objective To investigate the change of Th22 cells in peripheral blood of patients with Primary sjogren’s syndrome (pSS)and evaluate clinical significance.Methods 37 patients with pSS from January 2014 to November 2015 were enrolled the study as the observation group.Then 37 healthy adults receiving check-up during the same period were selected as con-trol groups in accordance with the proportion of 1∶1.Flow cytometry was performed to evaluate the levels of Th22 cells in peripheral blood,and tenzyme-linked immuno sorbent assay (ELISA)was used to measure the serum IL-22 levels.Pearson analysis was performed to investigate the relationship between Th22 level and serum IL-22 level,C3,C4,anti-SSA,anti-SSB, ANA antibody,EULAR Sjogren's syndrome disease activity index (ESSDAI)score in observation group.Then the levels of Th22 cells and serum IL-22 were compared among different labial gland pathologic stage in patients with pSS.Results The levels of Th22 cells and serum IL-22 in the observation group were (2.53±1.56)%,718.6±176.8 pg/ml respectively,and significantly higher than (1.24±0.51)%,258.9±72.4 pg/ml in control group (P<0.05).Th22 cell level was positively related with the level of serum IL-22,anti-SSA,anti-SSB and ESSDAI score,and negatively related with the content of C3 and C4 (P<0.05).There were no significant differences in the levels of Th22 cells and serum IL-22 different labial gland pathologic stage in patients with pSS (P>0.05).Conclusion The levels of Th22 cells and serum IL-22 significantly in-creased in patients with pSS,and related to other inflammatory indexes and disease activity,so they may participate in the genesis and development of pSS.

Objective To investigate the effect of nerve growth factor (NGF) and ciliary neurotrofic factor (CNTF) on the regeneration and functional recovery of sciatic nerves following sciatic nerve injuries (SNI). Methods The left sciatic nerves for 6 mm in 120 Wistar ratswere removed and divided randomly into 4 groups. Target muscular injection of NGF at concentration of 100 ng/kg and CNTF at 100 ng/kg was given to Group NGF+CNTF; CNTF at 100 ng/kg to Group CNTF; NGF at 100 ng/kg to Group NGF and equal quantity of normal saline to Group NS. Sciatic nerve functional index (SFI) measurement, morphometric analysis, S-100? and NF200 immunohistochemistry were performed for all the animals after operation. Results SFI, diameter and number of myelinated axons, and axonal quantity of S-100? and NF200 were significantly higher in Group NGF+CNTF than that in other groups. Conclusions Combined application of NGF and CNTF can enhance regeneration and functional recovery of sciatic nerves posterior to SNI.

AIM: To investigate the effects of aging on sperm maturation and fertility in mice. METHODS: Sperm of caput epididymides and cauda epididymides were obtained from Kunming mice aged 6 months (n=15, as control) and 18 months ( n=15). Sperm parameters including sperm density, viability, motility and nor mal morphological rate were recorded. Sperm of cauda epididymides was observed b y transmission electron microscope. The fertility potential and embryo developme ntal competence were performed by in vitro fertilization and embryo culture. RESULTS: Sperm motility, density and normal morphological rate i n aged mice were lower than those in control (P

Objective To investigate the changes of parathyroid hormone receptor in patients with different stages of renal disease and its possible underlying mechanisms. Methods Relatively quantitative RT/PCR method was used to detect PTH receptor mRNA expression in renal specimens obtained through biopsy or operation. Results The levels of PTH receptor mRNA were significantly decreased in all patients with chronic glomerulonephritisx moderate renal failure severe renal failure and acute renal failure, which was correlated with the extent of renal impairment. Conclusion The downregulation of renal PTH receptor occurs much earlier than the changes of renal function., plasma PTH., serum phosphate and calcium in the course of human renal disease.

To study the necessity and teaching evaluation of clinical medical teaching in dual language,we investigated the students of a five-year medical undergraduate in clinical medicine with a questionnaire.The attitude of medical students on necessity and teaching effect in bilingual teaching was surveyed and analyzed to provide the data and reference to effectively improve the bilingual teaching program.

Objective To explore a new modality of nursing care and convalescence guide for ambulatory (day) surgery of cervical disc herniation with CT guided multi-point radiofrequency (RF) thermocoagulation and target ablation. Methods 16 cases were reviewed. Results All the cases recovered satisfactorily without any complications. Conclusion The new modality of nursing care and convalescence guide is effective on RF for cervical disc herniation.