Fetal Blood ; Humans ; Infant, Newborn ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; blood ; congenital ; diagnosis

Laboratories ; manpower ; standards ; supply & distribution ; Pathology, Molecular ; manpower ; standards ; Quality Control

Acetylcysteine ; urine ; Humans ; Hydrocarbons, Brominated ; metabolism ; urine

Electronics ; organization & administration ; Health Promotion ; Humans ; Occupational Health Services

Automobiles ; China ; Humans ; Occupational Exposure ; Risk Assessment

Adult ; Aged ; Aged, 80 and over ; Carcinoma, Non-Small-Cell Lung ; genetics ; DNA Mutational Analysis ; methods ; DNA, Neoplasm ; genetics ; Female ; Humans ; Lung Neoplasms ; genetics ; Male ; Middle Aged ; Mutation ; Mutation Rate ; Receptor, Epidermal Growth Factor ; genetics

Blood Glucose ; Humans ; Hydrocarbons, Brominated ; toxicity ; Occupational Exposure ; adverse effects

Animals ; Connective Tissue Growth Factor ; metabolism ; Fibrosis ; Herbicides ; poisoning ; Lentivirus ; Lung ; pathology ; Paraquat ; poisoning ; Poisoning ; pathology ; Pulmonary Fibrosis ; pathology ; RNA Interference ; RNA, Small Interfering ; Rats

OBJECTIVETo construct pQE/Dnajb13 recombinant vector and induce the expression of the fusion protein.

METHODSThe open reading frame (ORF) of Dnajb13 gene was amplified from mouse testis cDNA library by PCR. The products were digested by SacI and SalI and subcloned into pQE vector. After identification by DNA sequence analysis, the recombinant plasmids were transformed into competent E.coli M15 cells. The His/Dnajb13 fusion protein was expressed with IPTG induction and purified with Ni(2+) affinity chromatography. Western blotting was used to detect Dnajb13 expression.

RESULTSThe recombinant vector pQE/Dnajb13 was successfully constructed. His/Dnajb13 fusion protein was expressed abundantly at 4 h after IPTG induction, and Western blot analysis demonstrated the presence of Dnajb13 expression in E.coli M15.

CONCLUSIONSWe have successfully constructed pQE/Dnajb13 recombinant vector, which may facilitate further investigation of the role of Dnajb13 in spermatogenesis.

Animals ; Blotting, Western ; Chromatography, Affinity ; Escherichia coli ; Genetic Vectors ; HSP40 Heat-Shock Proteins ; genetics ; Male ; Mice ; Plasmids ; Polymerase Chain Reaction ; Recombinant Proteins

Heuristic teaching is propitious to cultivating students' ideation. We have used different heuristic modes for example problems, stories, contrast, associationin and so on to cultivate medical students' ideation in pathobiology and immunology teaching and acquired good effect.