Autonomic nerve dysfunction is common after onset of ischemic stroke.Heart rate variability (HRV) has been used widely because of the regulation effect of heart rate by the quantitative analysis of the autonomic nervous system.This article reviews the evaluation method of HRV and its clinical significance in ischemic stroke.

The gastrointestinal motility in which clock genes play a role as molecular basis loses rhythm after spinal cord injury. This article reviewed the mechanism of impacts of acupuncture on the genes.

Congresses as Topic ; Pediatrics ; United States

Traditional Chinese Medicine (TCM), especially acupuncture and herbal medicine, is mainly applicated in musculoskeletal diseases, gynaecopathia, functional gastrointestinal diseases and cancer related pain in recent decade over the world. The influence of TCM on quality of life of pain patients received more attention, but still lacked evidence-based basis. Recent years, the basic studies were focusing on the function researches and tried to find out the relationship between functional units with systems biology methods to explain the holistic concept of TCM. After searching the database during 2002 to 2012, this article reviewed the character, development and innovation of TCM applicated in pain management.

Alveolar Process ; Housing ; Humans ; Incisor ; Maxilla ; Tooth Root

Objective To observe and compare the effects of 2-week YiJinjing Gong practice and pelvic traction in the treatment of acute low back pain(ALBP)in patients with lumbar intervertebral disc herniation(LIDH).Methods Sixty two patients with diagnosed LIDH accompanied with ALBP were randomly divided into YiJinjing Gong group (n=32,received YiJinjing Gong practice twice a day,45min per practice)and pelvic traction group(n=30,pelvic traction,once a day for 60 minutes).All of the treatments lasted for 2 weeks.Roland-Morris questionnaire(MRMQ) and ache vision analogy grading were used for evaluating the effectiveness of treatment before and after the treatment.Before the treatment,and 3 and 6 months after treatment,telephone survey was used to collect the condition of ALBP.Results(1) MRMQ dysfunction scores and visual analog scores were significant reduced after the treatment in both groups(P<0.01).MRMQ dysfunction score Was significantly improved in YiJinjing Gong group comparing to the traction group(P<0.05),but there was no significant difference in visual analog scores between the two groups (P>0.05).(2)Follow-up showed 1 recrudescence in YiJinjing group and 4 in traction group 3 months after the treatment;3 in YiJinjing group and 7 in traction group 6 months after treatment.Conclusion YiJinjing Gong practice can effectively improve patient's functional disturbance,quickly alleviate the LBP,and was superior to conventional pelvic traction in the treatment of LIDH patients with ALBP.

Objective To investigate the effects of mitochondrial DNA (mtDNA) on the pathopoiesis mechanisms of paraquat poisoning in vitro.Methods Firstly,the survival rate of A549 cells (human type Ⅱ alveolar epithelial cells) was measured with cell counting kit-8 after exposure to paraquat.Afterwards,the concentration of mtDNA in supernant of culture medium for culturing A549 and the chauge of mitochondrial membrance potential were detected with absolute quantitative PCR and confocal laser microscopy,respectively.Then,The chemotactic activity of mtDNA in peripheral blood mononuclear cells (PBMC) and neutrophils (PMN) were detected by transwell chemotaxis,and the subtype of chemotactic cells was measured with flow cytometry.Meanwhile,the role of mtDNA in vascular permeability was measured by using Xcelligence system and in vitro using vascular permeability kits.Finally,the effects of mtDNA in cell proliferation were to verify.Results The 50％ of lethal concentration (LD50) of paraquat for A549 was 600 μmol/L.Cell viability and concentration of mtDNA following challenge of PQ revealed in a concentration-and time-dependent manner (P ＜ 0.05).The mtDNA had a power in aggregating PBMC nonspecifically,but there was no effect on the vascular permeability was found.Moreover,the proliferation of human fibroblasts was not stimulated directly by mtDNA,but TGF-β1 (transforming growth factor-beta 1),a major pro-fibrotic factor,was increased compared to control group (P ＜ 0.05).Conclusions The mtDNA could play an important role in the inflammatory and proliferation responses to paraquat poisoning.

Objective To evaluate retroperitoneal laparoscopic adrenalectomy. Methods From June of 1999 to January of 2001, retroperitoneal laparoscopic adrenalectomy was performed on 52 patients with adrenal diseases, including 34 cases of aldosterone-preducing adenoma,3 nodular hyperplasia bilateral in 1, 5 Cushing's syndrom,1 Cushing's disease, 3 adrenal pheochromacytoma (bila- teral in 1), 4 nonfunctional adrenal adenoma, 1 myelolipoma and 1 metastasis carcinoma. Results 54 sessions of retroperitoneal adrenalectomy have been carried out for 52 patients (2 on both sides) with success in 51 procedures. The procedure was converted to open operation in 3 occasions because of bleeding or adhesion. The mean operation time was 135 minutes (40～270 min), and the estimated blood loss 45 ml (5～150 ml) with no need of transfusion. The postoperative hospital stay was 6 d (3～14 d) and the mean analgesia consumed 8.5 mg (0～50 mg) of morphine equivalents with no need of any analgesic at all in 17 patients. Conclusions Retroperitoneal laparoscopic adrenalectomy was less traumatic to the patients, with less postoperative discomfort and quicker recovery. The procedure should be considered as the first choice of therapy for benign adrenal diseases.

Background Brn-3a is a newly discovered specificity marker for retina ganglion cells(RGCs).It is well-known that RGCs damage is a important pathological basis of hypertension-visual disorder.But the study concerning expression of Brn-3a in RGCs in glaucoma eye is still rately.Objective The purpose of this work was to investigate the changes of Brn-3a expression in model eye with chronic high intraocular pressure(IOP) and its relation with morphology of retina and the expression of Brn-3a in chronic ocular hypertension rats.Methods Thirty-five clean adult SD rats were randomly divided into normal control group(5 rats) and model group(30 rats).Experimental chronic ocular hypertension models were induced unilaterally in the left eyes of 30 health adult SD rats by cauterizing super-scleral veins,and the conjunctival incision was made in the right eyes as sham operative group.The operated rats were subdivided into 6 groups according to the examination time points and 6 rats for each group.IOP was measured with Tono-Pen tonometer before and after 30 minutes,1,3,7,14,28 day after surgery respectively.The rat models were sacrificed in 1,3,5,7,14,28 days after operation by excessive anesthesia method,and retinal section was prepared for the histopathological examination and the RGCs were counted using Nissl staining method.Expression of Brn-3a in RGCs was detected by immunohistochemistry.This experimental complied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results The IOP was significantly raised from 30 minutes to 28 days after operation in model eyes with the top IOP( 34.46±4.65 )mmHg in the 30 minutes after operation,showing statistically significant differences in various time groups ( F =95.631,P =0.001 ) and different eyes ( F =287.473,P =0.001 ).Compared with sham operative group,the IOP were elevated from 1 day through 14 days after operation ( q =18.418,15.261,10.987,6.931,4.975,2.962,P ＜ 0.05 ).The numbers of RGCs were ( 29.08 ± 1.98 ) in the normal control group and decreased gradually by 3.17％,7.84％,14.60％,22.40％ in 1,7,14,28 day after surgery in the model eyes with the considerable differences in comparison with normal eyes ( t =5.943,8.034,15.023,17.004,19.371,P ＜ 0.05 ).Immunochemistry revealed that Brn-3a was specially expressed in the RGCs layer and the positive RGCs for Brn-3a were evidently decreased as the prolong of high IOP duration ( F =127.583,P =0.000 ).Conclusions Chronic glaucoma model can be successfully established using Shareef-Sharma method.The loss of RGCs is associated with the high lOP duration.The expression level of Brn-3a is a reliable index of high-IOP-induced damage of optical nurve.

The anesthetists not only pay attention to the exploitation and application of the information resource but also rely more and more on the convenience caused by the informatization. Based on HIS, LIS, and PACS, the Anesthesia information system has been constructed in order to guarantee the quality and safety of the anesthesia. The Anesthesia information system shares the information with HIS and LIS and manages the medical information such as the surgery and anesthesia closely. The paper introduces the notion of the anesthesia information system and its main function and also the predicted effect during the management of the anesthesia information system. Meanwhile, some problem about the application has been mentioned.