Adenocarcinoma ; metabolism ; pathology ; Aged ; Carcinoid Tumor ; metabolism ; pathology ; Chromogranin A ; metabolism ; Humans ; Immunohistochemistry ; Male ; Neoplasms, Multiple Primary ; metabolism ; pathology ; Phosphopyruvate Hydratase ; metabolism ; Rectal Neoplasms ; metabolism ; pathology ; S100 Proteins ; metabolism

Objective:This article has summarized the second generation cognitive screening exam(NCSE) about it's subtests,methods of use,applicability,characteristics and flaws.

A statistical analysis was conducted to evaluate the publication status in medical research of Shandong province in 2013, based on papers covered in SCI-Expanded database, aimed to provide an objective assessment of the level of medical research in Shandong Province.Factors involved in the analysis including medical papers authored by researchers in Shandong province, numbers and types of publication, distribution of author's location, institution, type of periodical, and impact factor.

Objective To explore the changes and the significance of left ventricular functions after blunt chest trauma(BCT)in rabbits.Methods 36 rabbit models of BCT with BIM-Ⅱ Bio-impactor in were used to observe the changes of left ventricular functions and free calcium,free calmodulin and total calmodulin were detected at pre-injury,the 2nd,4th,8th,12th and 24th hour after injury.Results The cardiac function were impaired.The systolic functions of left ventricle were impaired and recovered during 4-12 hour after BCT.The diastolic functions of left ventricle were impaired but not recovered 24h after BCT.The free calcium and total calmodulin in myocardial cells were increased from the 4th hour,reached,peak at the 8th hour post-BCT(P＜0.01),and then decreased,but were still higher at the 24th hour post-BCT than that of pre-BCT(P＜0.05).The free calmodulin in myocardial cells was low and reached peak at the 8th hour after BCT(P＜0.01).There is remarkable positive correlation between free calcium in myocardial cells and LVEDP and dp/dtmax with cardiac function(P＜0.05,P＜0.01).Conclusion The cardiac function is obviously changed after BCT,especially the diastolic functions of left ventricle.The hish concentration of free calcium in myocardial ceils may be one of the reasons of cardiac dysfunction after BCT.

?-tumor necrosis factor can enhance tumor cells autolysis and the expression of HLA antigenes. So we infected Human Hepatoma Cell Line (HHCL)with recombinant retroviral vector containing an insert of human tumor necrosis factor-? cDNA. The transduced tumor cells were isolated by G418 selection. After being irradiated by different doses of 60Co, their expression of TNF in 24 hours were tested. Observations of the relationship between the doses of irradiation and the expression levels of TNF were done. We also preserved the irradiated HHCL-TNF cells into liquid nitrogen, after recovering, tested their expressions of TNF again. This paper shows that: (l)following irradiation by 60Co, the production of ?-TNF persisted for about four weeks, and there is no difference among the different irradiated doses(40G~100G); (2)After being irradiated and kept into liquid nitrogen, the recovering HHCL-TNF cells constitutively secrete ?-TNF for about three weeks.These findings provide a logical basis for designing protocols for active immunotherapy in humans.

OBJECTIVE:To evaluate the compatible stability of cefpiramide sodium for injection with metronidazole in-jection.METHODS:Content,color and pH value of the mixed solution at different time within6hours after mixing under room temperature were monitored.RESULTS:There were no significant changes in content,color and pH value for the mixed solu-tion determined at different time within6hours after mixing under room temperature.CONCLUSION:The mixture of cefpi-ramide sodium with metronidazole injection can be used within6h after mixing.

Objective To explore the changed rules and the diagnositic methods of the cardisc functions after chest impact trauma(CIT).Methods The medals of moderate to severity CIT were established using BIM-Ⅱ Bio-impactor in 20 rabbits.The cardiac functions were examined with cardiac catheterization,single photon-emission computed tomography(SPECT)and the Doppler echocardiography at pre end post 1h,4h,8h and 24h after CIT.Results The cardiac functions were changed significantly after CIT.The expressions of the right ventricular dysfunctions mainly were systolic dysfuction while the left ventricular dysfunctions mainly were diastolic dysfunction after CIT.Conclustion All the cardiac catheterization、SPECT and the Doppler echocardiography are beneficial to the diagnosis of cardiac dysfunction afte CIT.The SPEGT is more exactitude and the Doppler echocardiography is more cheaper compared with non-invasive approaches.

Objective To observe the expression of fractalkine (FKN or CX3CL1) in serum and lung tissue in early phases after paraquat (PQ) poisoning in rats,and to analyze the effect of FKN on acute lung injury induced by PQ.Methods A total of 66 SD rats were randomly (random number) divided into two groups,namely PQ group (n =36) and control group (n =30).Through intra-peritoneal route,PQ (22 mg/kg) was administered to PQ group,and an equal volume of normal saline to control group instead.Rats were separately sacrificed at 6 h,12 h,24 h,72 h and 120 h after poisoning.Lung coefficient was determined.The levels of FKN in serum and lung tissue homogenate were detected by ELISA.Lung pathological changes were observed by HE staining.FKN changes were investigated by immunohistochemistry staining.Data was analyzed by SPSS 19.0.Results From 6 h to 120 h after poisoning,parameter determined in PQ group had great changes,compared with the control group.At 6 h,12 h,24 h,72 h and 120 h,lung coefficients in PQ group were 5.03 ±0.07,5.17 ±0.10,5.46 ±0.10,5.68 ±0.15 and 5.83 ±0.11,respectively,which were significantly higher than those (4.49 ± 0.20,4.28 ± 0.13,4.45 ± 0.17,4.31 ±0.19 and 4.31 ±0.16) in control group (P＜0.01).Levels of FKN (pg/mL) in serum were 140.9 ± 15.8,157.9 ± 17.6,188.8 ± 24.8,224.4 ± 18.1 and 229.9 ± 10.0,respectively,significantly higher than those (121.7 ± 12.8,121.6 ± 12.1,118.3 ± 14.0,122.8 ± 12.4 and 120.5 ± 8.8) in control group (6 h P ＜0.05,others P ＜0.01).Levels of FKN (pg/mL) in lung tissue homogenate were 4 222.0 ± 641.1,5 021.0 ± 514.5,5 911.6 ± 478.1,7 092.2 ± 652.9 and 7 639.3 ± 666.6,respectively,significantly higher than those (2 860.2 ± 477.3,3 068.9 ± 446.0,3 168.7 ± 728.5,3 178.0 ±488.2 and 3 147.3 ±426.6) in control group (P ＜0.01).In PQ group,pathological changes manifested themselves in inflammatory cell infiltration,congestion,edema,structural damage,etc.The lung injury aggravated gradually from 6 h to 120 h.In control group,there was no significant change.FKN expressed mainly in bronchial cells,alveolar epithelial cells and pulmonary artery endothelial cells.Where there was higher expression of FKN,there were more inflammatory cells infiltrated.The level of FKN in lung tissue homogenate was positively correlated with lung coefficient (r =0.937).The level of FKN in serum was positively related to that in lung tissue homogenate (r =0.968).Conclusion There is correlation between FKN and acute lung injury induced by PQ in rats.

Obstructive sleep disordered breathing (USDB) is a common diseases which caused by upper airway(UA) occlusion, muscle tone problems and collapse of upper airway etc. The article introduces how to select surgical treatment protocol. First, it is necessary of PSG and upper airway evaluation. Then, it is key to master surgical indications. For the children with hypertrophy of tonsillectomy or adenoidectomy, it may first step of tonsillectomy or adenoidectomy, they needed followed up by orthodontic doctor or orthognathic surgeon for tooth and maxillomandibular malformation. For the adult OSDB patients, there are two method, soft tissue reduction/mass excision around UA or maxillomandibular advancement(MMA), and MMA is recommended to the patients such as failure from UPPP and other surgical treatment, but for severe obesity patients, bariatric surgery is commended, it is very effective for soft tissue reduction around UA and weigh loss. For the OSDB patients with craniaomaxillomandibular deformities, such as craniosynostosis syndrome/micrognathia. Finally, it is important of the UA management for the surgical patients around perioperative period. All in all, it is necessary of the multidisciplinary cooperation for the OSDB patients, surgical treatment is useful method but it is not commend to all OSDB patients.
Adenoidectomy ; Adult ; Child ; Humans ; Hypertrophy ; Maxillofacial Abnormalities ; complications ; Sleep Apnea, Obstructive ; diagnosis ; etiology ; surgery ; Tonsillectomy

Objective:To investigate the clinical efficacy and safety of biopolysaccharide colloidal solution (Shutaishu) in the prevention of intestinal adhesion after abdominal surgery.Methods:A total of 100 patients scheduled to undergo abdominal surgery in Quzhou Municipal Hospital of Traditional Chinese Medicine from February to October in 2019 were included in this study. They were randomly divided into an observation group and a control group, with 50 patients in each group. Before closing the abdomen after surgery, abdominal cavity and wound were flashed with Shutaishu and 0.9% sodium chloride injection in the observation and control groups, respectively. The recovery of gastrointestinal function, intestinal adhesion, abdominal pain and the incidence of complications were compared between the observation and control groups.Results:The time to first regular bowel sound [(28.81 ± 5.56) h], time to first passage of flatus [(36.34 ± 6.24) h], time to first defecation [(41.65 ± 8.77) h], time taken for walking [(3.78 ± 0.64) d] in the observation group were significantly shorter than those in the control group [(32.75 ± 5.15) h, (41.51 ± 6.84) h, (48.05 ± 9.81) h, (4.27 ± 0.69) d, t = 3.68, 3.95, 3.89, 3.68, all P < 0.001]. At 5 and 30 days after surgery, the incidence of intestinal adhesion in the observation group [10.0% (5/50), 12.0% (6/50)] was significantly lower than that in the control group [26.0% (13/50), 30.0% (15/50), χ2 = 4.34, 4.88, both P < 0.05]. The total incidence of postoperative complications in the observation group was significantly lower than that in the control group [26.0% (13/50) vs. 54.0% (27/50), χ2 = 8.17, P < 0.05]. With time, visual analogue scale score was significantly decreased in both groups ( t = 3.51, 6.18, both P < 0.05). At 1, 3 and 5 days after surgery, visual analogue scale score in the observation group was significantly lower than that in the control group ( t = 4.07, 4.95 and 8.02, all P < 0.05). Conclusion:Biopolysaccharide colloidal solution is of high clinical value in the prevention of intestinal adhesion after abdominal surgery because it can promote the recovery of gastrointestinal function and early functional exercise, and has no obvious adverse reactions and complications.