1.Stem cells and repair of injured retina
yong, LIU ; dong-fu, FENG ; zhi-an, ZHU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(02):-
Recently,the progress in employing transplanting stem cells to cure injured retina is very fast and has been continuously yielding exciting results.Various sources are used in the studies,including retina-derived cells such as M?ller cells and ciliary body cells,and non-retina-derived cells such as embryonic stem cells and brain-derived stem cells.This review briefly discusses the recent progress of these studies.
2.Progresses in studies on allogeneic hematopoietic stem cell transplantation in children with acquired severe aplastic anemia.
Chinese Journal of Pediatrics 2013;51(5):345-348
Anemia, Aplastic
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mortality
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pathology
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therapy
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Child
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Disease-Free Survival
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Graft vs Host Disease
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prevention & control
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Hematopoietic Stem Cell Transplantation
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methods
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Humans
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Immunosuppressive Agents
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therapeutic use
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Risk Factors
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Severity of Illness Index
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Tissue Donors
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Transplantation Conditioning
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methods
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Transplantation, Homologous
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Unrelated Donors
3.Resveratrol improves intestinal injury in hemorrhagic shock rats by protection of mitochondria and reduction of oxidative stress
Shaofan KE ; Feng LIU ; Zhuanghui ZHU
Journal of Central South University(Medical Sciences) 2014;(12):1259-1265
Objective: To explore whether resveratrol can reduce intestinal damage in hemorrhagic shock rats and the underlying mechanism. Methods: A total of 24 Sprague-Dawley rats of speciif c pathogen free (SPF) were randomly divided into a control group(n=8), a resveratrol group (SR group,n=8) and a vehicle group (SS group,n=8). hT e mean arterial pressure was recorded. Two hours atf er hemorrhagic shock, 15 mg/kg resveratrol or 0.3 mL equal volume of vehicle and autologous blood were given, respectively. The intestinalspecimens were collected for hematoxylin-eosin (HE) staining and calculated the pathological score. The superoxide dismutase 2 (SOD2) and cytochrome C (Cyt C) protein expression was examined by immunohistochemistry and/or Western blot. ATP level, activities of glutathione peroxidase (GXH-px), catalase (CAT) and SOD were also detected. Results: Two hours atfer autologous blood transfusion, the mean arterial pressure in the SR group was signiifcantly higher than that in the SS group (P<0.01). Compared with the SS group, the pathological injury was signiifcantly alleviated and pathological scores were dramatically reduced in the SR group (P<0.05). hTe activities of GXH-px, CAT, SOD and the ATP levels in the SR group were signiifcantly higher than those in the SS group (allP<0.01). Compared with the SS group, the SOD2 expression was significantly higher while the Cyt C expression was dramatically lower in the SR group (both P<0.01). Conclusion: Resveratrol could alleviate the intestinal injury in hemorrhagic shock rats, which might be associated with its effects on reduction of oxidative stress and protection of mitochondria.
5.ACTH-independent Cushing syndrome(report of 89 cases)
Dingyi LIU ; Yongqi FENG ; Yu ZHU
Chinese Journal of Urology 2000;0(01):-
Objective To study the diagnosis and treatment of adrenocorticotrophic hormone (ACTH) independent Cushing syndrome. Methods A retrospective review was done on 89 patients with ACTH independent Cushing syndrome, including 85 cases of adrenal tumors with hypercorticism, 2 cases of adrenocortical adenocarcinoma and 2 cases with ACTH independent macronodular adrenal hyperplasia. Results Favorable results were observed from the surgery for 85 cases of adrenal tumors combined with hypercorticism.Two cases with ACTH independent macronodular adrenal hyperplasia were cured by bilateral adrenolectomy. Adrenal cortical adenocarcinoma was unresectable for both the patients who survived 7 and 9 months respectively. Plasma ACTH,dexamethasone suppression test,B ultrasonography and CT scan were useful for the diagnosis and differentiated diagnosis of ACTH independent Cushing syndrome. Conclusions Prognosis of adrenal tumors with hypercorticism and ACTH independent macronodular adrenal hyperplasia is favorable after surgery,whereas the prognosis of adrenal cortical adenocarcinoma is poor.
7.Isolation and antitumor activity in vitro of three kinds of bufadienolides
Dan LIU ; Lin ZHU ; Jianfang FENG
Chinese Traditional Patent Medicine 1992;0(06):-
AIM: To isolate three kinds of bufadienolides composition from Venenum bufonis in order to investigate their antitumor activity in vitro.METHODS: Alcohol extraction and isolation by Silica gel colume were employed in this study.Their antitumor activities in vitro were evaluated by MTT colorimetric method.RESULTS: With such methods,three kinds of bufadienolides,including Bufalin,cinobufagin,and resibufogenin with purity above 90% were prepared.The in-vitro results showed that three kinds of bufadienolides had prominent inhibition effect on human lung cancer A549 cells,human breast cancer MDA-MB-435 cells in the range of 0.001 ?g/mL ~ 100 ?g/mL.CONCLUSION: Isolation by Silica gel colume can be used to separate three kinds of bufadienolides from Venenum bufonis and its constituents has prominent anti-cancer effects.
8.Greenlight Photoselective Vaporization of the Prostate for Benign Prostatic Hyperplasia in Aged Patients: A Report of 90 Cases
Liang ZHU ; Xining JI ; Feng LIU
Chinese Journal of Minimally Invasive Surgery 2005;0(10):-
Objective To evaluate the efficacy of greenlight photoselective vaporization of the prostate(PVP) for benign prostatic hyperplasia(BPH) in aged patients.Methods A total of 90 aged patients with BPH underwent PVP with an 80 W KTP laser(Laserscope Company) under sacral or peridural anesthesia.Results The operative time was(42.5?18.4)min;the postoperative urinary catheter time was(2.5?0.8)d.Qmax increased significantly from(4.8?1.2)ml/s preoperatively to(18.6?1.4)ml/s postoperatively(t=22.566,P=0.000).International prostate symptom score decreased from(25.5?2.4)points preoperatively to(13.2?1.9)points postoperatively(t=12.286,P=0.000).Residual urine decreased from(76.3?9.4)ml preoperatively to(16.5?2.4)ml postoperatively(t=21.901,P=0.000).Transient dysuria occurred in 4 cases and postoperative delayed hemorrhage in 2 cases.No urinary incontinence occurred.Conclusions PVP for BPH is a safe and effective procedure,and suitable for aged and high-risk patients with BPH.
9.Disseminated penicillium marneffei infection:two case reports and review of literature
Yueniu ZHU ; Ying LIU ; Feng CHEN ; Xiaodong ZHU
Chinese Pediatric Emergency Medicine 2011;18(5):427-429
Objective To discover the clinic presentation and therapy of disseminated Penicillium marneffei infection in children.Methods We reported the clinic presenation,pathology and therapy of the disseminated Penicillium marneffei in children.Results Case one,l-year-old boy presented with fever and cough for one month.Penicillium marneffei was cultured from bone marrow and blood.Case two,8-year-old girl presented with delirium.Penicillium marneffei was cultured from blood.Treatment with voriconazole solely was successful in the first case.Conclusion Central nervous system could been infected by Penicillium marneffei.Treatment with voriconazole solely could be effective in children.
10.lntralamellar cryolysis of cornea with amniotic membrane transplantation on painful bullous keratopathy
Jiang, ZHU ; Hui-Feng, LIU ; Bin-Liang, ZHU
International Eye Science 2015;(1):134-135
AlM:To evaluate the effect of intralamellar cryolysis of cornea with amniotic membrane transplantation on painful bullous keratopathy ( PBK) .?METHODS: Randomly selected 156 cases ( 156 eyes ) with PBK who underwent surgery of intralamellar cryolysis of cornea with amniotic membrane transplantation. Followed up with 2 ~ 3mo, the symptoms of eye pain, corneal epithelial blisters, foreign body sensation and postoperative complications were observed.?RESULTS:Pain symptoms disappeared in all patients, and corneal epithelial blisters disappeared in 130 cases (83. 3%). All agonizing pain symptoms disappeared, but patients had occasional foreign - body sensation, occasional corneal epithelial blisters in 24 cases ( 15. 4%) . Two weeks after surgery, corneal stroma dissolved, 2 cases ( 1. 3%) of them were cured by conjunctival flap cover.?CONCLUSlON:The operation of intralamellar cryolysis of cornea with amniotic membrane transplantation can relieve the pain in 98. 7% of PBK patients and simple therapy for treating PBK. Hence, it's be worth to advocate for relieve the pain of patients.