Objective To study the levels of interleukin(IL)-2 ,interferon-γ(IFN-γ) and IL-10 in peripheral blood and the effec-tive concentration of lactate dehydrogenase(LDH) in serum of patients with acute leukemia .Methods 65 patients with acute leuke-mia were divided into non-remission group(n=35) and remission group(n=30) .50 healthy volunteers were used as control group . The levels of IL-2 ,IFN-γ ,IL-10 in peripheral blood were measured by enzyme linked immunosorbent assay(ELISA) .The effective concentration of LDH was measured by automatic biochemical analyzer .Results The levels of IL-2 ,IFN-γin non-remission group were significantly decreased compared with control group and remission group(P<0 .05) ,but the level of IL-10 in non-remission group was significantly increased compared with control group and remission group(P<0 .05) .The effective concentration of LDH in non-remission group was significantly higher than that in control group or remission group(P<0 .05) .Conclusion The levels of IL-2 ,IFN-γand IL-10 in peripheral blood and the effective concentration of LDH in serum of patients with acute leukemia have changed .Clinical detection of IL-2 ,IFN-γ,IL-10 and LDH may contribute to the early diagnosis of patients with acute leukemia .

Objective To investigate the diagnostic threshold value and the influencing factors of high‐sensitivity troponin I measurement in the diagnosis of patients with acute myocardial infarction .Methods Collecting 9 236 patients who are the first time hs‐cTnI testing in the internal emergency department of our hospital(including 9 099 patients with non‐AMI and 137 patients with AMI) as participants ,then dividing non‐AMI patients into elder group(>60 years old) and younger group(≤60 years old) ,hs‐cTnI concentrations were compared between the elder and the younger group with different gender .The optimal threshold value of hs‐cT‐nI for acute myocardial infarction was determined by receiver operating characteristic(ROC) curve of AMI patients with different age and gender .Results The hs‐cTnI concentrations of non‐AMI patients in the elder male group were 39 .21 ng/L [(37 .48-40 .93)ng/L] ,which was higher than those of non‐AMI patients in the younger male group 22 .38 ng/L[(21 .16-23 .6)ng/L] and the difference was statistically significant(P<0 .05) ,the same result was seen in the elder female group and the younger female group .The total male group was 31 .26 ng/L [(30 .17 -32 .34)ng/L] ,it was higher than the total female group 26 .8 ng/L [(25 .73-27 .86)ng/L] .According to the ROC curve ,the optimal threshold value for AMI diagnosis was 45 ng/L in total AMI group and 135 ng/L in the elder male group and 45 ng/L in the elder female group ,which was different with the cut off value(120 ng/L) .Conclusion the hs‐cTnI levels were related with age and gender ,it is very important for us to consider these factors when we make the optimal threshold value with our own data .

Objective: To study the protective effect of ? carotene in anoxia injured cardiomyocytes. [WT5FZ]Methods: The cultured rat neonatal cardiomyocytes were subjected to 2h anoxia and the changes of lactate dehydrogenase(LDH) activity, malondialdehyde (MDA), oxygen free radical (OFR) contents and superoxide dismutase (SOD) activity were detected. The effects of pre treatment with ? carotene were then investigated. Results: The LDH, MDA, OFR and SOD of cardiomycytes were changed significantly after 2h anoxia (P

Objective To observe the clinical change of cytokines expression in peripheral blood in patients with allergic asthma and its significance .Methods Forty patients with allergic asthma and thirty healthy controls were enrolled .The concentrations of IL‐17 ,IL‐22 and IL‐10 in peripheral blood were measured by ELISA ;the serum IgE levels were measured by electrochemical lumi‐nescence method ;The eosinophil proportion was detected by blood cell analyzer ;the IL‐17 ,IL‐22 ,IL‐10 and the severity of asthma were conducted Pearson correlation analysis .Results The level of serum IgE and eosinophil proportion was significantly higher in patients with allergic asthma compared with the controls (P<0 .05);compared with the control group ,the concentration of IL‐17 and IL‐22 in peripheral blood of patients with allergic asthma were increased ,but the expression of IL‐10 were decreased (P<0 .05);the level of IL‐17 and IL‐22 were positively correlated with severity of allergic asthma (P<0 .01) ,but the level of IL‐10 was negatively correlated with severity of allergic asthma (P<0 .05) .Conclusion The expression of IL‐17 ,IL‐22 and IL‐10 play an im‐portant role in the pathogenetic mechanism of allergic asthma ,and a dynamic monitoring could be helpful for the diagnoses and treatment of allergic asthma .

OBJECTIVE: To observe the changes of intracellular free calcium level ([Ca(2+)]i) in gallbladder cells of guinea pigs with gallstones so as to study the mechanisms of gallstone formation and the prevention and treatment function of traditional Chinese herbs for nourishing the liver. METHODS: Eighty guinea pigs were randomly divided into four groups, which were normal control group, untreated group, nourishing-liver Chinese drug (NLCD) group and ursodeoxycholic acid (UDCA) group, with 20 guinea pigs in each group. Gallstones were induced in the guinea pigs of the latter 3 groups by the feed of diet inducing cholelithiasis with high cholesterol, while the corresponding medicines were used in NLCD group and UDCA group for prevention and treatment for 7 weeks. Then the state of the guinea pigs, the formation of gallstones, and the changes of [Ca(2+)]i in gallbladder cells were observed. RESULTS: The [Ca(2+)]i in gallbladder cells of guinea pigs in the untreated group was decreased significantly. NLCD improved the behavioral signs of the guinea pigs, significantly decreased the formative rate of gallstones and increased the [Ca(2+)]i in gallbladder cells. CONCLUSIONS: The [Ca(2+)]i in gallbladder cells is the important factor for contractile function of gallbladder and the information of gallstones. Traditional Chinese herbs for nourishing the liver may significantly increase the [Ca(2+)]i in gallbladder cells to facilitate contraction of the smooth muscle cells of gallbladder and relieve the cholestatis. It may be one of the mechanisms of traditional Chinese herbs for nourishing the liver in preventing and treating cholelithiasis.

Objective To improve the diagnosis of tuberculosis ( TB) by analyzing Mycobacterium infection in fine-needle aspiration biopsy specimens from children with tuberculous lymphadenitis .Methods Fine-needle aspiration biopsy was performed on 269 children with tuberculous lymphadenitis diagnosed by Shanghai Public Health Clinical Center from January 2011 to September 2013 .The needle aspiration biopsy specimens were processed for acid-fast bacillus (AFB) smear test, mycobacterial culture and Mycobacterium identification ( p-nitrobenzoic acid inhibition test ) .Results Cytological diagnosis of tuberculous lymphade-nitis was made for 269 patients.The positive results by AFB smear test were detected in 63.19% of 269 specimens (n=170) and 40.15%(n=108) specimens were positive in mycobacterial culture .The differ-ence between the two tests were significant (P<0.01).The positive rate of Mycobacterium detected by using BACTEC MGIT 960 automated system and L?wenstein-Jensen culture method were 38 .66% ( n=104 ) and 28.99%(n=78), respectively, showing the significant difference between two tests (P<0.05).AFB smear test in combination with mycobacterial culture could precisely diagnose 70.63% of tuberculous lym-phadenitis in children.Of the 108 clinical isolates, 105 strains (97.2%) were Mycobacterium tuberculosis complex and the rest were non-tuberculous Mycobacterium strains (2.8%).Conclusion The positive rate by AFB smear test was significantly increased in fine needle aspiration biopsy specimens after a series of treatments including sample digestion , centrifugation and precipitation , but the positive rate of mycobacterial culture was reduced .Diagnostic accuracy could be significantly improved by using BACTEC MGIT 960 sys-tem.Mycobacterium tuberculosis complex was the predominant pathogenic bacterium in children with tubercu-lous lymphadenitis .

Objective To investigate CT in combination with MRI in diagnosing and differential diagnosing chondrosarcoma.Methods CT and MRI findings pathologically in 19 cases of chondrosarcoma proved were analyzed retrospectively.Results On CT,the tumors appeared as bony destruction in 15 cases,soft tissue masses in 14 cases,and with multiple calcifications in different shape.Postcontrast CT scan demonstrated peripheral and septal enhancement in 2 cases,and inhomogeneous enhancement in 3 cases.On MRI,the chondrosarcomas were hypointense or isointense on T1WI,heterogeneous hyperintense on T2WI.Postcontrast MRI demonstrated peripheral and septal enhancement with wreath-or honeycomb-like appearance in 9 cases,heterogeneous enhancement in 2 cases,obvious enhancement in 2 cases.On fat-suppression T2WI,the boundary and size of lesions were demonstrated clearly.The phylloid and cauliflower-like shape of tumors were displayed better on sagital and coronal position.Conclusion CT and MRI are both important in diagnosis and differetial diagnosis of chondrosarcoma,the combination of the two methods may improve the diagnosing accuracy of this tumor and minimize misdiagnosis.

Objective To investigate the relationship between serum nitric oxide (NO) and bone metabolism in streptozotocin induced early diabetic (STZ DM) rats. Methods Twenty SD rats were divided into 2 groups, 12 STZ DM rats and 8 controls. Fasting blood glucose, HbA 1c , serum insulin, bone mineral density (BMD) (whole body, lumbar and femoral bone), bone metabolic parameters 〔such as serum calcium, vitamin D 3, parathyroid hormone (PTH), calcitonin, osteocalcin, and urinary pyridinoline/creatinine〕, as well as serum NO were measured. Results Compared with the controls, serum NO in STZ DM group significantly elevated 〔(51.3?11.9 vs 38.1?12.0)?mol/L, P

Objective To investigate the diagnostic Value of multi-slice spiral CT 3-Dimensional reconstruction in intestinal obstruction.Methods 28 Patients with malignant lesions of colon underwent volume scanning using multislice helical CT.Four types of reconstruction included multiple planner reconstruction(MPR),CT virtual colonoscopy(CTVC),shaded surface display(SSD)and raysum.The results were compared with those of colonoscopy and patholog.Results CT diagnostic accurate rate was 100%,and the accurate rate of the cause was 96% in intestinal obstruction,including primary or secondary neoplasms(18/28),abhesions(2/28),bowel torsion(2/28)and external or internal hernias(3/28) and gallstone ileus(3/28).Conclusion There were advantages and disadvantages in diagnosis of mechanical intestinal obstruction with MPR,CTVC,SSD and Raysum respectively.The combined use of the four post-processing technigues can be more helpful to precise localizing and qualitative diagnosis of lesions.MPR should be the optimal post-processing technigues for the diagnosis of intestinal obstruction

BACKGROUND: Amniotic fluid cells have been widely used in antenatal diagnosis for gene mutation-related diseases. However,there are few reports concerning isolation, culture, surface character identification, differentiation and application perspective of fetal mesenchymal stem cells (MSCs) derived from amniotic fluid.OBJECTIVE: To investigate the differentiation of fetal mesenchymal stem cells (MSCs) derived from second-trimester amniotic fluid into osteoblasts in vitro.DESIGN, TIME AND SETTING: The cytological/n v/tro study was conducted at the Experimental Center of Xinhua Hospital from August 2005 to May 2006.MATERIALS: Ten amniotic fluid samples were obtained from pregnant women (18-22 weeks after conception) or aborted women,The informed consents were obtained from pregnant women.METHODS: Fetal MSCs were separated mechanically from amniocyte culture system and expanded in medium in vitro. At passage 3, fetal MSCs were induced in 100 nmol/L dexamethasone, 10 mmol/L β-glycerophosphoric acid and 50 mg/L vitamin C for 14 days.MAIN OUTCOME MEASURES: Collagen Ⅰ and alkaline phosphatase expression was detected by immunohistochemistry.Collagen Ⅰ protein expression was determined by Western Blot analysis. Calcium tuberoses were measured by Von Kossa staining. The cytoskeletal protein was detected by laser confocal microscopy.RESULTS: The isolated fetal MSCs were uniformly positive for CD44 and HLA-ABC, negative for CD34, CD45 and HLA-DR. After being induced with osteogenic medium for 14 days, 91% cells were positive for alkaline phosphatase, and 87% cells for collagen Ⅰ.Cells expressed collagen Ⅰ protein. Number of calcium tuberoses was increased and became big over time. Cytoplasm microfilament presented green fluorescence and the microfilament surrounding cells formed dense bundle.CONCLUSION: Fetal MSCs derived from amniotic fluid could be induced into osteeblasta and displayed a typical osteoblastic morphology and biological characteristic.