ObjectiveTo investigate the efficacy of intravenous parecoxib combined with femoral nerve block on postoperative analgesia and knee functional rehabilitation in elderly patients following total knee replacement.MethodsForty elderly patients with osteoarthritis schedu[ed for total knee arthroplasty under general anesthesia,aged 65-74 years,weighing 45-90 kg,ASA [Ⅱ,were randomly divided into two groups( n ＝ 20 each).FNB group received femoral nerve blockade for postoperative analgesia ( local injection of 0.25 ％ ropivacaine 25 ml at the end of surgery,repeated every 8 h up to 72 h after surgery).FNB-Pa group received the femoral nerve blockade plus the parecoxib (administered Ⅳ bolus of 40 mg 30 min before the end of surgery,12,24,and 48 h after surgery respectively).Pain was scored at rest or during continuous passive motion using Visual Analog Scale (VAS).The Hospital for Special Surgery (HSS) knee score was determinated before surgery,at 24,48 and 72 h after surgery respectively.Side effects were also recorded in both groups.Results VAS score was significantly lower at rest and during continuous passive motion in FNB-Pa group than in FNB group ( P < 0.05).Compared with baseline value,HSS score increased significantly during postoperative analgesia in both groups( P < 0.05 or 0.01 ).HSS score were significantly higher at 48 h and 72 h after surgery in FNB-Pa group than in FNB group ( P < 0.05).No severe related side effects occured in both groups.ConclusionFemoral nerve block in combination with intravenous parecoxib can safely and effectively relieve the static and dynamic pain after total knee replacement and is helpful for rehabilitation of the prosthesis knee joint function after surgery.

Based on the present situation analysis of nursing experimental teaching for undergraduate,reform was carried out through our innovating experimental teaching philosophy,integrating experimental teaching curriculum,compiling experimental teaching materials,establishing experimental teaching inspection and assessment system and consummating management mechanism of experimental teaching center,and some developing thoughts were also proposed.

CYP3A plays central roles in the oxidative and reductive metabolism of a variety of enogenous as well as exogenous compounds, it is very important to study about regulation of CYP3A geneexpression. This article reviews the recent development of molecular mechanism of regulation of CYP3A gene expression and CYP3A mutation. The relationships between CYP3A mutant and some diseases are also introduced.

Objective To probe the transplantation of skeletal muscle satellite cells of new-born rats for repairing skeletal muscle contusions. Methods Thirty-six Sprague-Dawley adult rats were separated at random into experimental and control groups (including a normal saline control group and a normal control group). Contusions were created on the left hind legs of rats in the experimental group. The cells were cultured and antibodies were identified through immunocytochemical staining and fluorescence labeling. In the experimental group, 0.2 ml of a muscle satellite cell suspension was slowly injected into the medial and lateral heads of the right calf gastrocnemius. The normal saline control group was injected with an equal volume of normal saline solution at the same sites. On the 5th, 10th and 15th day after the operation, EMG wave-amplitude and wave-width were recorded. The recovery rate of the muscle's wet weight and the gray value of a muscle fiber transverse section were determined.Two-factors analysis of variance was performed on the data. Results Before transplantation the muscle satellite cells were bright red after staining and their nuclei were blue after fluorescence labeling. After the operation the number of cells fluorescing and the strength of the fluorescence gradually decreased. The transplanted cells formed myotubes, melted into the muscle tissue and gradually took part in the restoration. The stained muscle satellite cell nuclei were free in intercellular space and melted into the basement membrane gradually. The outlines of the muscle tissue became progressively clearer. EMGs showed the fibrillation potentials as well as positive sharp waves reduced, but they too tended to become similar to those from the normal side. Restoration of EMG wave-amplitude and wave-width in the experimental group was better than in the saline control group and close to those in the normalcontrol group. The gray value of the muscle fiber transverse section area under HE staining was significantly lower at the 5th day post transplantation in both the experimental group and the saline control group than in the normal control group. At the 10th and 15th days post transplantation, the average gray value in the experimental group had increased significantly more than in the saline control group, and it was close to the normal control group average. In the experimental group the recovery rate of muscle wet weight was close to 1, but in the saline control group it was less than 1. Conclusions Transplanting skeletal muscle satellite cells of new-born rats can produce evident effects in renovating injured muscles.

Objective:To observe the clinical effects of acupuncture at Jiaji (EX-B 2) points plus tuina manipulation for thoracic facet joint disorder, and get new clinical evidence for treatment of thoracic facet joint disorder. Methods:Totally 106 patients with thoracic facet joint disorder were randomly allocated into an observation group and a control group based on the random number table. Patients in the control group were treated by tuina manipulation, while those in the observation group were treated by acupuncture at Jiaji (EX-B 2) points before tuina manipulation. Patients in the two groups were treated once a day. The improvements of signs and symptoms and the efficacy were observed after 3 treatments. Results:After treatment, there were intra-group statistical differences in scores of 8 signs and symptoms in both groups (P<0.01); the score of each item in the observation group was lower than that in the control group, and there was statistical significance in the inter-group difference (P<0.05). The cure rate of the observation group was 50.9%, versus 26.4% in the control group, and there was a significant difference between the two groups (P<0.05). Conclusion:In treating thoracic facet joint disorder, acupuncture plus tuina manipulation can restore the biomechanical balance of thoracic vertebrae, fully maximized the combined effect, and significantly improve the clinical efficacy.

REG4,as a member of regenerating gene family,involves in proliferation and differentiation of gastrointestinal tract cells.Recent studies have shown that REG4 gene expression is upregulated in gastrointestinal cancer,pancreatic cancer,gallbladder cancer and prostate cancer,and associated with tumorigenesis,metastasis and poor prognosis of these carcinomas.Further studies have indicated that not only does REG4 play an important role in the diagnosis of gastrointestinal carcinoma,but also is closely linked with the effects of radiotherapy and chemotherapy.

Objective To study the deep vein thrombosis (DVT ) in lower extremities of patients with or without cardiovascular disease after fracture .Methods Five hundred and eighty-four frac-ture patients were divided into cardiovascular disease (CVD) group (n=235) and non-cardiovas-cular disease (NCVD) group (n=349) .Patients in CVD group were further divided into subgroup A (with essential hypertension) ,subgroup B (with CHD) ,and subgroup C(accompanying essen-tial hypertension and CHD) .Incidence of DVT in CVD group ,NCVD group ,subgroups A-C at different ages after fracture was compared .Results The incidence of DVT was significantly high-er in CVD group than in NCVD group (P<0 .01) ,in two groups on days 0-7 after fracture than on days 8-14 or >14 after fracture ,and in patients with their age ≥60 years than in those with their age <60 years .Conclusion DVT is easier to occur in CVD patients with their age ≥ 60 years than in those with their age <60 years after fracture .DVT usually occurs within 1 week af-ter fracture .Importance attached to high risk population and measures taken as soon as possible against it can reduce DVT after fracture .

In order to understand the prevalence of the obesity in children and adolescence and provide the basic information for developing scientific strategy for obesity prevention,this paper described the common methods to evaluate the obesity of children and adolescence,and its prevalence,distributions of population and tendency of changing.The paper also analyzed the effects of sex,age,region and socioeconomic status on obesity.

Objective To investigate and analyze the clinical efficacy of high dose hCG combined with psychological intervention in threatened abortion. Methods 50 patients with threatened abortion in our hospital from February 2015 to October 2016 were selected,and divided into study group and control group,25 cases in each groups. The study group for the first time the dosage of 10000U, after the dose to 5000U, use drug 2 times every week, and at the same time, the implementation of the corresponding psychological nursing intervention; patients in the control group for the first time the dosage was 2000U, after every day of medication 1 time, each time the dosage is still 2000U, to observe and compare the therapeutic effect. Results The patients in the study group, the success rate of pregnancy was significantly higher than the control group, while the patients in the study group, β -hCG and progesterone levels were significantly higher than those in the control group, while the study group after the nursing care of patients with anxiety and depression scores were significantly lower than the control group, the differences between the groups was obvious (P<0.05). Conclusion High dose hCG combined with psychological intervention in threatened abortion is more effective and effective in treatment of threatened abortion.

OBJECTIVE:To investigate the role of clinical pharmacists in pediatric severe infection treatment,and to provide reference for rational drug use in clinic. METHODS:Based on related typical cases,the breakthrough points of clinical pharmacists providing pharmaceutical care in pediatric severe infection treatment plan were introduced. RESULTS:Clinical pharmacists formu-lated multi-drug resistance pathogenic antibacterial plan according to the results of pathogenic microbiological examination,use pharmacokinetics/pharmacodynamics,excluded interference to pediatric anti-infection treatment from ADR,and guideline and eco-nomical factors of patients. CONCLUSIONS:Clinical pharmacists adopt professional knowledge of pharmacy to provide pharmaceu-tical care in pediatric severe infection treatment and play professional assisted role so as to guarantee safe,effective and economical treatment.