Standardized management for medical equipment department of the second-class traditional Chinese medical hospital is of great importance to enhance working and economic efficiencies. When standardized management performed, it's suggested that related organization, regulation as well as maintenance and file management be involved in.

Objective:To study the influence of hepatocellular carcinoma cells microenvironment on the biophysical characteristics of mature dendritic cells(mDCs)and explore the immune escape mechanism of tumor cell from interdiscipline viewpoint.Methods:The monocytes were then cultured in RPMI1640/10% FBS supplemented with 100 ng/ml rhGM-CSF and 100 ng/ml rhIL-4 for 7 days to develop into immature DCs(imDCs).mDCs was induced by addition of 20 ng/ml rhTNF-? to imDCs for another 3 days of culture.Acquired mDCs were co-cultured with human HCCs for 48 hours in Transwell.The treated cells were investigated the viscoelastic property,membrane fluidity and transendothelial migration by micropipette,fluorescence polarization and Transwell,respectively,untreated cells as control.Results:Compared with untreated DCs,the DCs co-cultured with HCC appeared decreased viscoelastic property,membrane fluidity and impaired transendothelial migration.Conclusion:HCCs can impaire the biophysical characteristics of mDCs leading to their deteriorated transendothelial migration.This might be one aspect of escape from immune surveillance of tumor.It is significant for further understanding the immune escape mechanism of tumor.

Antibodies, Monoclonal ; administration & dosage ; Antigens, CD20 ; drug effects ; metabolism ; Antineoplastic Agents ; administration & dosage ; Drug Delivery Systems ; methods ; Fusion Proteins, bcr-abl ; drug effects ; metabolism ; Genetic Therapy ; Humans ; Neoplasms ; metabolism ; pathology ; therapy ; Receptor, ErbB-2 ; drug effects ; metabolism

Diagnosis, Differential ; Humans ; Neoplasms ; classification ; genetics ; pathology ; Survival Analysis ; Terminology as Topic ; World Health Organization

In this paper, we designed an oxygen saturation, heart rate, respiration rate monitoring system based on smartphone of android operating system, physiological signal acquired by MSP430 microcontroller and transmitted by Bluetooth module.
Cell Phone ; Equipment Design ; Heart Rate ; Humans ; Monitoring, Physiologic ; instrumentation ; Oxygen ; Respiratory Rate ; Software ; Wireless Technology

Adenocarcinoma ; classification ; genetics ; pathology ; Adenocarcinoma, Bronchiolo-Alveolar ; genetics ; pathology ; Exons ; Humans ; Lung Neoplasms ; classification ; genetics ; pathology ; Mutation ; Oncogene Proteins, Fusion ; genetics ; Proto-Oncogene Proteins ; genetics ; Proto-Oncogene Proteins p21(ras) ; Receptor, Epidermal Growth Factor ; genetics ; ras Proteins ; genetics

Percutaneous transluminal coronary angioplasty (PTCA) is a very effective approach to treat coronary artery disease.However,restenosis after PTCA affects 40% to 60% of patients in the months after an initially successful intervention.Although a number of new techniques and pharmacological approaches have been tried to reduce the rate of restenosis,only a few have shown even preliminary efficacy.Radiation therapy seems to provide an interesting,nonpharmacological approach to prevent the restenosis after PTCA during recent years.The experiments and clinical data of this new approach are reviewed in this paper.

Ligustrazine is one of the active ingredients of chuanxiong. Modern medical research indicated that ligustrazine had the effect of improving microcirculation, anti-inflammatory, anti-fibrosis, which showed the potential function of ligustrazine in the prevention and treatment of postoperative abdominal adhesions. The basic theory of traditional Chinese medicine (TCM) also believed that ligustrazine had the unique double effect of regulating both qi and blood. Therefore, ligustrazin had broad research and application prospects in the prevention and treatment of postoperative abdominal adhesions. This article reviewed studies of ligustrazine in the prevention and treatment of postoperative abdominal adhesions in recent years from four aspects, which were theoretical basis, clinical application, mechanism research and pharmaceutical dosage forms. It identified the research status and advantages of ligustrazine in the prevention and treatment of postoperative abdominal adhesions. It also pointed out shortages in the clinical application, such as unstandardized medication, small sample size study and etc. There were insufficient deep and clear studies in the research of mechanism. In the aspect of pharmaceutical dosage forms, the thinking should be expanded with innovation. It will provide basis and direction for research and application in the future, in order to further elucidate mechanisms in the prevention and treatment of postoperative abdominal adhesions as well as provide theory basis, research thinking and methods for the real display of advantages in TCM.

0.05),but significant difference existed on the 7th day after injury. The Cor content of group Arg was noticeably lower than that of group EN on each time point(P

Objective To study the influence of microenvironment created by dissoluble cytokines derived from hepatocellular carcinoma cells (HCCs) on the functional status of mitochondria in dendritic cells (DCs) in order to investigate the mechanisms of tumor immune escape.Methods CD14+ monocytes,which were isolated with immune magnetic beads from fresh peripheral blood of healthy human,were then cultured in RPMI1640/10% FBS supplemented with 100 ng/ml rhGM-CSF and 100 ng/ml rhIL-4 for 7 d to develop into immature DCs (imDCs).Maturation was induced by addition of 20 ng/ml rhTNF-? to imDCs for another 3 d of culture.Acquired imDCs and mDCs were respectively co-cultured with human HCCs for 48 h in Transwell chamber.These treated cells were investigated for mitochondrial membrane potential,enzyme activity and intracellular Ca2+ concentration ([Ca2+]in) in the cytoplasma by immune fluorescence and MTT assay.Those untreated cells served as control.Results After co-cultured with HCCs,flow cytometry showed that the mitochondrial membrane potentials of imDCs and mDCs were respectively decreased from (659.991?17.052)and(473.741?11.676)to(482.681?7.935)and(407.189?5.051)(P