1.The value of detecting mycoplasma pneumonia antibody and total serum IgE in patients with pneumonia in children
International Journal of Laboratory Medicine 2015;(14):2073-2074
Objective To discuss clinical value of the detection of Mycoplasma pneumoniae IgG with antibody (MP‐IgG) and the serum total IgE in child pneumonia .Methods Detected the serum M P‐IgG and total IgE in 480 cases of hospitalized children pa‐tients with pneumonia ,and grouped by the results of MP‐IgG .Results There was significantly difference between each category in study group and control group on the positive rate and concentration of serum total IgE(P<0 .05) ,comparison of the positive rate and concentration of serum total IgE between each category in study group had statistically significant differences(P<0 .05) .There was a positive correlation between the positive rate and concentration of IgE with the titer of MP‐IgG(R2 was0 .957 1 and 0 .917 7 respectirely) .Conclusion The combined detection of serum MP‐IgG and total IgE could provide reference for diagnosis of Myco‐plasma pneumonia and basis for clinical treatment .
2.Characteristics of motor neuron differentiation from stem cells
Chinese Journal of Tissue Engineering Research 2009;13(49):9739-9742
Studies have shown that embryonic stem cells and neural stem ceils could be induced to differentiate into motor neurons by adding inducing factors to the culture to improve differentiation proportion of motor neurons. The development and maturation process of motor neurons in vitro culture is similar to that in embryo, involving participation of Pax6, Nk×6.1, Olig2 and HB9. Moreover, cells with HB9 as separation mark almost differentiate into motor neurons in vitro. In addition, other types of protein marks have been used for identification of motor neuronal induced differentiation, such as neuron mark MAP-2, β-tubulin-Ⅲ, cholinergic cell mark ChAT, VAChT, Schwann cell mitogen REG2, and voltage-dependent calcium ion channel 11.2 subunit. The identification of induced differentiation of motor neurons also involves detection of function of differentiated motor neurons by two methods. One method is to detect the function of in vitro cultured motor neurons, and the other is to detect the function of transplanted motor neurons in vivo. Although induced differentiation of stem cells into motor neurons becomes mature, many issues remain in clinical application, such as cell sources of induced differentiation of motor neuron, efficiency of induced differentiation, purification of differentiated motor neurons, and function of motor neurons.
3.Meta-analysis of fusion with and without instrumentation in the treatment of chronic low back pain
Chinese Journal of Orthopaedics 2008;28(8):628-633
Objective To evaluate the efficacy of fusion with and without instrumentation in the treatment of chronic low back pain.Methods To search Medline(from 1966 to Deeember.2006).Embase (1984 to December,2006),Cochrane Central Register of Controlled Trial f4th Quarter 2006),Current Controlled Trials.The China Biological Medicine Database(1984 to December,2006).and hand searched several related journals.The quality of included trials was evaluated.Data were extracted by two reviewers independently with a designed extraction form RevMan 4.2.8 software was used for data analysis.Results Eight studies involving 656 patients were included.The results of meta-analysis indicated that statistically significant difierences were observed between the two operative procedures in the fusion rate(OR=2.26,95% CI 1.51-3.38,P=0.0001),clinical outcome(OR=1.54,95%CI 1.09-2.19,P=0.01),incidence of re-operation (OR=2.16.95% CI 1.08-4.33,P=0.002).There were no statistically significant difierences in back pain scores (WMD=-0.22,95% CI-0.81-0.36),leg pain scores(WMD=0.22,95%CI-0.50-0.93),complications(OR=1.68,95%CI 0.78-3.63),satisfaction of surgery by patients(OR=1.54,95%CI 0.93-2.55).Three studies described the mean surgical time,perioperative blood loss,days in hospital after surgery,which revealed that single fusion was superior to fusion with instrumentation.Three studies described the work status of patients post-operatively.Two studies indicated smoking could decrease the fusion rate.Conclusion To compare with single fusion,fusion with instrumentation can increase the fusion rate and improve clinical outcome slightly,but it also increases the incidence of re-operation.More high quality large-scale randomized controlled trims are required.
4.Expression of clusterin during the nervous functional rehabilitation of rats after acute spinal cord injury
Chinese Journal of Tissue Engineering Research 2006;10(28):167-169
BACKGROUND: Clusterin has many biological functions, especially the great protection effects on histiocytes in pathological conditions. It will be helpful to discuss the expression of clusterin in the spinal cord injury tissue to further identify the mechanism of secondary spinal cord injury and to provide possible treatments.OBJECTIVE: To observe the expression of clusterin in acute spinal cord injury tissue.DESIGN: Randomized controlled animal study.SETTING: Department of Orthopaedics, First Affiliated Hospital, China Medical University.MATERIALS: The experiment was performed in Experimental Animal Department, China Medical University from January 2003 to January 2006.A total of 65 adult healthy SD rats of 250-300 g provided by Experimental Animal Department, China Medical University were selected. METHODS: All the rat models by modified Allen assay for acute spinal cord injury were randomized into injury group, sham operation group and normal control group with 30, 30 and 5 rats in each group, respectively.The rat injury models in dorsal spinal cord were prepared by modified Allen assay: the target injury site was T10 segment; the sham operation group was given T10 total laminectomy. The normal control group received no operation. Rats in the injury group and sham operation group were killed at hour 12, days 1, 3, 7, 14 and 21 after spinal injury and operation with 5 rats at each time point, respectively. Frozen sections of the injury part were made at different time point after injury. Degeneration and necrosis of spinal cord injury tissues were observed with hematoxylin-eosin (HE) staining. Deposition and disposition of positive reactants of clusterin were observed with clusterin immunohistochemical staining. Average gray value of positive reactants of clusterin was detected with semi-quantitative image analysis (went with the intensity of clusterin immunoreaction in inverse ratio).MAIN OUTCOME MEASURES: ①Degeneration and necrosis as well as positive expression of clusterin of spinal cord injury tissues of rats in each group. ②Average gray value of clusterin positive reactants in spinal cord injury tissues of rats in each group.RESULTS: A total of 65 rats were involved in the result analysis, without dropout. ①In the injury group, positive expression of clusterin began to increase at day 1 after injury, reached peak at about day 7, declined gradually at day 14 and tended to be stable at day 21 after injury. This dynamic course of change was along with the prolongation of time. The expression of clusterin appeared in the sham operation group and normal control group at each time point, and the expression was stable. There was no dynamic change with the prolongation of time. ②In the injury group average gray value of positive reactants of clusterin was significantly lower at days 1, 3,7, 14 and 21 as compared with that in the sham operation group and normal control group (t=6.33-15.57, P < 0.01 ). There was no significant difference in average gray value of positive reactants of clusterin at each time point in the sham operation group as compared with the normal control group (P > 0.05).CONCLUSION: The expression of clusterin increases greatly in acute spinal cord injury tissues, and has dynamic change, which is the same with spinal cord injury.
5.Tumor associated gene PFTK1
Journal of International Oncology 2013;(2):100-102
PFTK1,an important member of cyclin dependent kinase Cdc2 family,is over expressed in many malignancies.PFTK1 involves in multiple processes,such as regulation of cell cycle,tumor invasion and metastasis,and the Wnt signal transduction pathway,which has a significant impact on prognosis of tumor.
6.PROLIFERATIVE EFFECT OF INSULIN-LIKE GROWTH FACTOR-1 ON MUSCLE-DERIVED STEM CELLS
Acta Anatomica Sinica 1953;0(01):-
Objective To explore the effect of insulin-like growth factor-1(IGF-1) on the growth of muscle-derived stem cells(MDSCs). Methods MDSCs were isolated from the hindlimb muscle of new born mice through serial preplates.DMEM with 2% fetal bovine serum was used to induce MDSCs to differentiate into skeletal muscle lineage.The expression of stem cell marker Sca-1 or skeletal muscle cell marker ?-sarcomeric actin was examined by immunocytochemistry SP method.MTT colorimetric microassay was conducted to determine the effect of IGF-1 on MDSCs proliferation.The relationship between functioning and culture time and the relationship between functioning and IGF-1 concentration were studied. Results MDSCs were successfully isolated from the hindlimb of neonatal mice.Over 90% of the MDSCs showed Sca-1 positive immunoreactivity.MDSCs could give rise to ?-sarcomeric actin positive myotubes in differentiation cultures.The proliferative effect of IGF-1 on MDSCs increased gradu ally with prolonged culture time.The elevation of IGF-1 concentration also increased the proliferative effect until it came to a saturation point.Conclusion IGF-1 can promote the proliferation of in vitro cultured MDSCs.
7.Analysis of related factors for tracheotomy after cervical spinal injuries
Chinese Journal of Trauma 2003;0(12):-
Objective To investigate the risk and prediction factors for tracheotomy after cervical spinal injuries. Methods A retrospective analysis was done on 1 064 cases suffering from cervical spinal injuries, of which, according to nerve function evaluation criteria of American Spinal Injury Association (ASIA), there were 243 cases at Grade A,327 at Grade B, 306 at Grade C and 188 at Grade D. Except for seven cases with brain injuries, trachea injuries, with a tracheotomy before hospitalization, a total of 106 cases needed tracheotomy during their hospitalization. The following factors were evaluated to predict the possible causes for tracheotomy: segment of injury, ages, smoking history, past diseases (such as diabetes mellitus, hypertension and coronary heart disease) and complicated chest or lung injuries. Results The highest rate of tracheotomy for cervical spinal injury at Grade A was 35.0% (85/243), while the rate of tracheotomy for incomplete injury at Grades B, C and D was only 2.6% (21/814). Of cervical spinal injury at Grade A, all C_3 segment injuries needed tracheotomy. Of all, the percentage for C_4 and C_5 segment injuries accounted for 43.2% (105/243),of which the percentage for tracheotomy was 74% (63/85). The possibility of tracheotomy decreased gradually from below C_5. Besides C_3 segment injuries, C_4 segment injuries had the highest possibility of tracheotomy, with statistical difference compared with other segments (P
8.Advances in endostatin research
Chinese Journal of Pathophysiology 2000;0(07):-
Endostatin, a 20 kD (184 aa) C-teminal fragment of collagenⅩⅧ,is the most potent inhibitor of tumor angiogenesis described so for. Endostatin was initially isolated from a murine hemangioendothelioma cell line (EOMA). Purified recombinant murine endostatin generated in E.coli bacteria injected as unfolded suspension, inhibited the growth of a varity of metastatic and primary tumors in mice. However, its widespread application has been hampered by difficulties in the large-scale production of the antiangiogenic proteins. The limitation may be resolved by in vivo delivery and expression of the antitangiogenic gene. This review summarized the advances in endostain research in recent years including structure, the mechanism of generating ,function and therapy.
9.Liver transplantation for chronic viral hepatitis B and C
Chinese Journal of Hepatobiliary Surgery 2000;6(4):246-252
Liver transplantation is considered an effective form of therapy for patients with end-stage liver disease.Unfortunately the results of transplantation for patients with chronic viral hepatitis have not been as promising as for other liver disorders.A high rate of hepatitis recurrence in the allograft leading to a higher incidence of graft and patient loss have led many transplant centers to reconsider the use of orthotopic liver transplantation(OLTX)in these groups.Current strategies to prevent hepatitis B infection focus on identifying groups of hepatitis B virus(HBV)patients with lower risks of reinfection,specifically those with lack of markers for active HBV replication.Trials to convert"high risk"HBV patients to a "low risk"group have recently been initiated in the "high risk"group of patients with the aim to convert their HBV status from replicating to nonor low-replicating states.Antiviral agents or immunostimulatory therapy before and/or after OLTX have been proposed as a means to minimize recurrence of hepatitis B in the liver allograft.While the focus has been specific issues related to prevention or treatment of viral hepatitis in OLTX patients,there are still a number of areas for investigation.For example,immunosuppression is considered to play a role in the recurrence and chronicity of HBV and hepatitis C virus(HCV)in the liver allograft,especially since corticosteroids have been demonstrated to have a biologic effect on HBV by inducing HBV antigen synthesis.Moreover,most of the results to date are based on the use of cyclosporin A as the primary agent of immunosuppression.Tacrolimus(FK506)based immunosuppression may also prove to have a beneficial effect for HBV patients undergoing OLTX.By minimizing or eliminating the need for corticosteroids,FK560 based immunosuppression may lessen the risk of recurrence or the severity of hepatitis in the liver allograft in HBV patients,and ultimately improve patient and graft survival.The controversies in the use of OLTX for HBV and HCV have persisted,in spite of an appreciation for the risks associated with selection,prophylaxis and treatment of candidates with HBV,principally dut to the uncertainties associated with OLTX for HCV.The routine application of OLTX to HBV candidates with low or no levels of HBV replication appears justified,however patients with active HBV replication should undergo OLTX only in a setting where new strategies for prevention, prophylaxis or treatment are being tested.Recently,the U.S.Department of Health and Human Services has approved OLTX for HBV,reflecting the improvements made in the clinical arena.However,further advances are needed for HCV prophylaxis and treatment of recurrent hepatitis following OLTX.