To study the effect of total matrines and extracts from Periplaneta americana on negative endometrial cancer cell JEC of progesterone receptors. After detecting the effect of total matrine, extracts from P. americana and their combination on JEC cells' growth inhibition, cell cycle, P53 and c-erbB-2 gene protein expressions through MTT, flow cytometry instrument and Western blot method, the author found that, (1) MTT: total matrines and extracts from P. americana could inhibit the growth of JEC cell, with significant increase in the inhibitory effect in the combination group. (2) Flow cytometry instrument: the cell cycle at G0/G1 increased after the treatment with total matrines, the cell cycle at G2/M increased after the treatment with extracts from periplaneta americana, and the ratio of G0/G1 cell cycle in the combination group was significantly higher than the other groups, with inhibition in cell growth and statistical difference in inter-group comparison (P < 0.05). (3) Western blot: the expression level of P53 increased and c-erbB-2 decreased after the treatment with total matrines, extracts from P. americana and their combination on JEC cell, with statistical difference in inter-group comparison (P < 0.05). The above results suggested that total matrines, extracts from P. americana and their combination could induce cell cycle arrest and inhibit the growth of JEC cell by up-regulating P53 and down-regulating the c-erbB-2 level.
Alkaloids ; therapeutic use ; Animals ; Cell Line, Tumor ; Endometrial Neoplasms ; chemistry ; drug therapy ; pathology ; Female ; Flow Cytometry ; Humans ; Periplaneta ; Phytotherapy ; Plant Extracts ; therapeutic use ; Quinolizines ; therapeutic use ; Receptors, Progesterone ; analysis ; Tumor Suppressor Protein p53 ; analysis ; physiology

DNA, Viral ; genetics ; Hep G2 Cells ; Hepatitis B virus ; drug effects ; Humans ; Oligonucleotides, Antisense ; pharmacology

OBJECTIVE: To identify two different characters of Imperatae Rhizoma on a molecular level. METHODS: A total of 30 samples of Imperatae Rhizoma were analyzed using DNA barcoding method based on ITS1, ITS2, psbA-trnH, matK, and rbcL genes. The DNA sequences were further used for site difference analysis. RESULTS: There is no site difference on psbA-trnH, matK, rbcL of two different characters of Imperatae Rhizoma, three key site differences on ITS1, and one key site difference on ITS2. The key site difference on ITS2 among two different characters of Imperatae Rhizoma can be recognized by the restriction enzyme HpyCH4III. CONCLUSION: ITS2 /ITS1 regions can accurately and efficiently identify two different characters of Imperatae Rhizoma, and PCR-RFLP analysis of the ITS2 region with HpyCH4III is a method to distinguish two different characters of Imperatae Rhizoma.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Male ; Mucopolysaccharidosis II ; genetics ; Pedigree

OBJECTIVE To investigate genes associated with aminoglycosides modification enzymes(AMEs) in Pseudomonas aeruginosa(PAE) isolated from ICU patients.METHODS Drug-reisistant genes encoding AMEs such as aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ were detected by polymerase chain reaction(PCR)(amplification) in 21 PAE isolates.RESULTS The positive rates of aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ,ant(2″)-Ⅰ and aac(3)-Ⅰ genes were positive in 19.0%,23.8%,9.5%,4.8%,19.0% and 0% of 21 isolates,respectively. Drug-resistant genes encoding AMEs were detected positively in 42.8% of 21 isolates.(CONCLUSIONS) AMEs genes are present in high percentage of PAE isolated from ICU patients.

OBJECTIVETo assess the feasibility and safety of robot-assisted laparoscopic radical prostatectomy (RLRP) in the treatment of prostate cancer.

METHODSUsing the da Vinci robot surgical system, we performed RLRP for 34 patients with localized prostate cancer and analyzed the intraoperative and follow-up data.

RESULTSThe procedures were performed successfully in all the patients, with the mean operation time of 198 min (range 135-340 min), average blood loss of 257 ml (range 50-700 ml), and 1 case of blood transfusion, but no postoperative complications. Three cases had positive surgical margins. Postoperative examination at 4 weeks showed PSA > 0.2 microg/L in 2 cases, suggestive of residual tumor, for which maximal androgen block therapy was administered. The other 32 patients were followed up for 3-10 (mean 7.5) months, during which the average level of serum tPSA remained < 0.2 microg/L. Urinary continence was found in 94% (32/34) and 97% (33/34) of the patients at 3 and 6 months, respectively, of whom 77% (26/34) and 88% (30/34) had no urinary leakage (0 pad per day).

CONCLUSIONRLRP, with its advantages of less perioperative blood loss, low rate of positive margin, and good urinary continence, is a safe and effective surgical option for the treatment of prostate cancer.

Aged ; Humans ; Laparoscopy ; methods ; Male ; Middle Aged ; Prostatectomy ; methods ; Prostatic Neoplasms ; surgery ; Retrospective Studies ; Robotics

Dendritic Cells ; cytology ; immunology ; virology ; HIV ; physiology ; HIV Infections ; immunology ; Humans ; Immunity, Mucosal ; immunology ; Mucous Membrane ; cytology ; immunology ; Virus Attachment ; Virus Internalization

Animals ; Anti-HIV Agents ; pharmacology ; HIV ; drug effects ; genetics ; physiology ; HIV Infections ; drug therapy ; immunology ; virology ; Humans ; Mucous Membrane ; immunology ; virology ; Virus Replication ; drug effects

Animals ; Cells, Cultured ; Hepatocytes ; drug effects ; secretion ; Male ; Pancreatic Polypeptide ; pharmacology ; Rats ; Rats, Sprague-Dawley

Aim: To explore the effect of urolithin A (UA) on autophagy of cardiomyocytes in diabetic mice. Methods: Glucose (5. 5, 20, 33, 47 mmol · L-1) and UA at different concentrations were used to stimulate the neonatal rat cardiomyocytes for 48 h. MTT assay was used to detect the survival rate of cardiomyocytes. Western blot was used to detect the expression of autophagy proteins LC3 and p62. Healthy male C57BL/6 mice were intraperitoneally injected with STZ for 6 days to establish diabetic mouse model. UA was given by intragastric administration for 10 weeks and the cardiac function indicators were detected by ultrasound. Masson staining was used to observe the myocardial pathological sections. Results: After treatment with 33 mmol · L-1 of high glucose for 48 h, the survival rate of cardiomyocytes decreased, and autophagy protein p62, LC3 II, the ratio of LC3 II/I increased, which were reduced with UA treatment. Compared with the control mice, the cardiac function of diabetic cardiomyopathy mice showed decreased ratio of E/A peak. Masson showed that cardiac fibrosis expression decreased after UA administration. Conclusion UA can protect diabetic cardiomyopathy by affecting myocardial autophagy.