Objective To establish the Valproate (VPA) exposure-induced autism spectrum disorder (ASD) model at early pregnancy in rats,and to study the changes of social interaction test and neuroimmune system in autism model rats,and discuss the pathogenic mechanism.Methods Twelve-week-old rats were randomly divided into the non-exposed group (n =10) and the VPA-exposed group (n =10).Their babies were respectively just the normal control group (n =10) and the model group (n =10).The autism-like social behavior was evaluated via the social interaction test.The level of interleukin (IL)-1β,IL-4,IL-6,interferon-gamma (IFN-γ),transforming growth factor-beta (TGF-β) in mothers and offspring were detected by using enzyme-linked immunosorbent assay (ELISA).The histopathological damage in brain was observed with immunohistochemical staining.Results The score of social interaction test in the model group [(-163.16 ± 101.92) scores]was lower than that in the normal control group [(132.73 ± 114.63) scores] (t =-6.100,P ＜ 0.05).The levels of IL-1β,IL-4,IL-6,IFN-γ in VPA-exposed group were higher than those in the non-exposed group (t =5.883,6.394,5.655,5.393,all P ＜ 0.05),while the level of TGF-β was lower than that in the non-exposed group (t =-6.726,P ＜ 0.05).The levels of IL-1β,IL-4,IL-6,IFN-γ in the model group were higher than those in the normal control group(t =3.058,3.048,6.670,5.486,all P ＜ 0.05),while the level of TGF-β was lower than that in the normal control group (t =-6.516,P ＜ 0.05).The change trend of the level of cytokines in the serum of ASD model rats was similar to the change trend of the level of cytokines in the serum of VPA exposed rats.The result of social interaction test the approach-avoidance score was inversely correlated with the levels of IL-1β and IL-4 (r =-0.802,-0.781,all P ＜ 0.05).The result of immunohistochemical staining showed the expressions of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (IBA1) in the model group were higher than those in the normal control group.Conclusions The main neuroimmunological pathogenesis mechanism can be explained as follows:at early pregnancy,the maternal cytokines skewing influenced by VPA exposure can make immune activation,induce immune system dysfunction and affect the brain growth and development,which result in autism-like behavior in offspring.

Objective To explore the diagnostic value of multi-slice spiral CT MIP-MPR image in regional lymph node metastasis of colonic carcinoma.Methods CT findings of 33 cases with pathological confirmed colonic carcinoma were analyzed retrospectively . All patients were underwent three-phase 256-slice CT dynamic enhancement scanning.All data were processed in workstation,to obtain routine MPR and MIP-MPR image.The regional lymph nodes of colon cancer were counted,and divided into 3 groups accord-ing to the short diameter of lymph node:group I (3.0-5.0 mm),group Ⅱ(5.1-10 mm),and group Ⅲ(10.1 mm up).The diag-nostic sensitivity of CT MPR and MIP-MPR image in checking out lymph nodes of different groups was compared by paired test.Re-sults The average value(x ±s )of lymph node number of three groups with routine MPR and MIP-MPR image were:group I, 3.66±1.99,6.09±2.44 (t=1 1.6,P <0.05);group Ⅱ,3.72±1.79,4.03±1.89 (t=3.73,P <0.05);group Ⅲ,1.21±1.31, 1.27±1.46(t=1.44,P >0.05).There were statistical differences in group I and group Ⅱ.Sensitivity of MIP-MPR image in chec-king out regional small and middle lymph nodes of colonic carcinoma was higher than routine MPR image.Conclusion Multi-slice spiral CT MIP-MPR image is a good way to checking out regional small and middle lymph nodes of colonic carcinoma.

Objective To clone rat neuroglobulin (NGB) gene and construct its eukaryotic expression vector. Methods The total RNA was extracted from Wistar rat brain and the full length cDNA encoding NGB was obtained by RT-PCR. After the sequence was confirmed by sequencing and BLAST, it was inserted in the eukaryotic expression vector pCDNA3.1 (+), then the sequence and reading frame were confirmed by two restriction endonucleases and sequencing. Results The NGB gene was cloned with four bases mutated and its eukaryotic expression vector was constructed. Conclusion NGB expressed in Wistar rat brain. NGB gene was successfully cloned and inserted in eukaryotic expression vector.

Objective To observe the protective effect of recombinant plasmid pCDNA3.1(+)/Ngb during focal cerebral ischemia in rat brain.Methods Fifty-four male Wistar rats were randomly divided into three groups:normal saline(NS)control group,plasmid control group,and recombinant neuroglobulin group.NS,plasmid pCDNA3.1(+)and recombinant plasmid pCDNA3.1(+)/Ngb were respectively injected into two sites of the rat cerebra1 cortex 24 hours before induction of neocortical focal ischemia by occlusion of the right middle cerebral artery for 24 hours.The condition of local ischemic damage,expression of bcl-2 and the apoptosis in neural cells were confirmed by staining with 2% 2.3.5-triphenyltetrazolium chloride,in-site cell apoptosis detection,indirect immunofluorescent staining and Western blotting,respectively.Results The extent of cerebral infarction tissue and the apoptosis cells in the pCDNA3.1(+)/Ngb group were significantly reduced than those in other control groups(P

Objective To develop the darkroom(tent) needed for X-ray film developing in the field conditions.Methods Three layers of colored cloth in red,black and army green were used for light-proving.Methods as field tent was used to support ground and create space.Results Tested in comprehensive field medical support drilling,this tent could develop film in 10 minutes despite the high temperature in the tent.The result was accepted by doctors.Conclusion It is waterproof,windproof and lightproof,and can develop satisfactory films in practical drill.

As the intake routes and hazards to human health of heavy metals were well recognized,therefore the intake amount of heavy metals through foodchain has been effectively controlled and cut down. In many cases,oral ingested soil has become an important source of heavy metals in human. The traditional approach for estimating the bioavailability of heavy metals in oral ingested soil was animal (in vivo)test. Compared with animal test,in vitro test possesses several advantages including satisfactory replicability,time and cost effectiveness. Also,the results of in vitro tests showed well correlation to those of in vitro tests. The progress of development,current status and the methodology of in vitro tests in evaluating bioavailability of heavy metals in contaminated soil via oral intake were reviewed in this article. In vitro test could be used not only to estimate the bioavailability of heavy metals in soil in order to provide information for establishment of soil quality or clean-up criteria with regard to human health,but also to evaluate the effectiveness of soil remediation technologies. The in vitro test has been employed in many developed countries due to its above mentioned advantages. The use of in vitro test for screening of contaminated soil and evaluating the effectiveness of remediation technology is believed to be promising.

AIM: To study the pharmacodynamics of Guanhuang Ganmao Capsule. METHODS: Antiviral effect of Guanhuang Ganmao Capsule was investigated by infecting chicken embryo with N_1H_1; its antipyretic action was evaluated with rabbit pyrogentic test induced by bacterial endotoxin; anti-inflammation by rats swelling test with injection of 1% agar and mice abdominal capillary permeability test with dimethylbanzene; the antitussive activities were investigated through testing cough times induced by ammonium hydroxide and sulfur dioxide in mice; the expectorant activities of it were studied by testing the amount of phlegm secreted in mice; The antibacterial effects in vivo were evaluated in mice with induced systematic infection. RESULTS: Guanhuang Ganmao Capsules had obvious antiviral action, antipyretic action, anti-inflammation and antitussive action. It took expectorant effect. The capsules markedly reduced death rate of staphylococcus aureus infection in mice. CONCLUSION: Guanhuang Ganmao Capsules possess antiviral; antipyretic; antitussive and expectorant effects as well as anti-inflammation

Objective To investigate the status of AmpC β-lactamase and extended spectrum β-lactamases (ESBLs) production and drug-resistance characteristic of Enterobacter cloacae.Methods Enterobacter cloacae was isolated and cultured from variety of specimens from April 2005 to July 2007. VITEK32 automatic microbial analytical system was applied to performing identification of bacteria and susceptibility test. ESBLs were confirmed by NCCLS double disc assay, and three-dimensional test was used to detect AmpC.Results A total of 63 strains of Enterobacter cloacae were isolated, including 13(20.6%) single AmpC producing strains, 24(31.8%) single ESBLs producing strains, 6(9.5%) both AmpC and ESBLs producing strains, and 20(38.1%) neither AmpC nor ESBLs producing strains. The resistance of lactamases producing strains was significantly higher than that of lactamases non-producing ones. Especially in both AmpC and ESBLs producing strains, the drug resistance was serous.Conclusion The prevalence of AmpC β-lactamases and/or ESBLs producing strains is a higher level in Fuyang area. The drug resistance of AmpC β-lactamases producing Enterobacter cloacae isn′t identical to that of ESBLs producing one. Different categories of antibacterials should be chosen according to the different types of producing lactamases.

Objective To investigate the relationship between CT performance of Hver cirrhosis and liver fibrosis factors.Methods 89 hospitalized patients were graded for the CT performance of the liver cirrhosis:O class (have no cirrhosis),Ⅰ,Ⅱ,Ⅲ,Ⅳ class.Then to compare the examination data of senlm liver fibrosis factors:type Ⅲ procollagen(Prop),Ⅳ collagen(Ⅳ-C),laminin(LN),hyaluronie acid(HA).Results O class(have no cirrhosis in the sufferer):four index signs all has no increase.Ⅰ class:the Ⅳ-C slightly goes up.Ⅱ class:Ⅳ-C,the HA slishtly goes up.Ⅲ class:the three of Ⅳ-C,HA,LN rises,the HA is distinct.Ⅳ class:four index signs all go up obviously.Conclusion With the CT performance of liver cirrhosis is obvious,the hver fibrosis factors get obvious,too.Both of them get positive correlation.

Serum prealbumin (PAB) and plasm free amino acids were determined in normal and zinc deficient preschool children. In zinc deficient children, the prealbumin level was much lower than normal;cystine and .proline were higher but most of other amino acids and essential amino acids, branched chain amino acids, essential amino acids/total amino acids, branched chain amino acids/nonbranched chain amino acids were signifcantly lower than those of normal children.