Forty-one type 2 diabetic patients with fasting plasma glucose≥15 mmol/L and postprandial plasma glucose≥16.8 mmol/L underwent short-term intensive insulin treatment (IIT). Glucose load tests were performed before and after treatment in these patients and proinsulin and C peptide were assayed by RIA. The results showed that IIT decreased the levels of fasting and postprandial proinsulin and improved the ? cell function.

Objective To evaluate effects of two different flap designs (envelope flap and triangular flap) on complica?tions after the mandibular third molar surgery. Methods A randomized, self controlled clinical trial design was selected for 52 patients treated in the outpatient surgery of Stomatological Hospital Affiliated to Tianjin Medical University. Patients were treated with envelope flap design for lower third molar removal in one side and triangular flap on the other side. VAS scores were used to evaluate postoperative pain. The postoperative swelling was evaluated by patient`subjective index. The degree of the upper and lower incisor distance was used to evaluate trismus. Data of postoperative swelling, pain and trismus were re?corded 1, 2 and 7 days after surgery. Data of postoperative wound dehiscence, bleeding situation and alveolitis were also re?corded and compared between two groups. Results There were no significant differences in postoperative pain after 1, 2 and 7 days between two flap designs (P>0.05). After 1 and 2 days there was more severe facial swelling in triangular flap group than that of envelope flap group (Z=2.005, Z=2.017, P<0.05). There was more limited mouth opening after 1 and 2 days in triangular flap group than that of envelope flap group (t=2.578, t=2.410, P<0.05), but on the seventh day there was no significant difference in the severity of facial swelling and trismus between two groups (P>0.05). There were no signifi?cant differences in postoperative pain, alveolitis, bleeding and wound dehiscence between two groups (P > 0.05). Conclu?sion The envelope flap is more conductive to the early recovery in patients after surgery, but in the long term there is no ob?vious difference between the two flap designs.

The reproductive toxicity of environmental endocrine disruptors has attracted substantial attention from researchers in recent years. Bisphenol A (BPA) is among the most prominent environmental estrogens worldwide, demonstrated to be related with the impairment of male reproductive function as well as other health problems, such as diabetes, obesity, cardiovascular diseases, and cancer. BPA acts primarily by mimicking antiandrogenic and estrogenic effects, disturbing the hypothalamic-pituitary-testicular axis and modulating gene expressions and enzyme activities in the hormone biosynthesis affecting steroids or its receptors. BPA is also involved in DNA methylation and the effects of epigenetics, resulting in dyszoospermia, oligoasthenoteratospermia/azoospermia and/or infertility in males. This review addresses the effects of BPA on male reproductive function, focusing on the mechanisms of its toxicity on spermatogenesis, semen quality, and the reproductive system.
Benzhydryl Compounds ; toxicity ; Endocrine Disruptors ; toxicity ; Endocrine System ; drug effects ; Environmental Pollutants ; toxicity ; Estrogens ; toxicity ; Humans ; Infertility, Male ; chemically induced ; Male ; Phenols ; toxicity ; Semen Analysis ; Spermatogenesis ; drug effects

Bone Morphogenetic Proteins ; therapeutic use ; Calcitriol ; analogs & derivatives ; therapeutic use ; Electric Stimulation Therapy ; methods ; Humans ; Osteogenesis ; Osteogenesis, Distraction ; classification ; methods ; Transforming Growth Factor beta ; therapeutic use ; Vitamin D ; analogs & derivatives

BACKGROUND:Recently, the effects of lumbar facet joint morphology on the onset of degenerative lumbar spondylolisthesis have been a discussion point. However, the discussion mainly concerned CT manifestations of joint angle and osteoarthritis. Studies have shown that the changes in cross-sectional area of paraspinal muscle were considered as the essential marker for the pathological diagnosis of degenerative lumbar spondylolisthesis. However, previous studies limited the accuracy of the conclusion due to smal sample size, young age, and measurement.
OBJECTIVE:To observe changes in MRI images of bilateral paraspinal muscles after degenerative lumbar spondylolisthesis.
METHODS:This study adopted the method of retrospective study, selected 80 cases of degenerative lumbar spondylolisthesis as lumbar spondylolisthesis group, and 80 healthy persons as control group. Image J software was utilized to measure cross-sectional areas of bilateral erector spinae and psoas major muscle on the L 3-5 vertebral endplate levels using T2-weighted MRI in both groups. The ratio of relevant data was calculated.
RESULTS AND CONCLUSION:Compared with the control group, the ratio of cross-sectional area of paraspinal muscle was increased on the same side and the same level in the lumbar spondylolisthesis group (P<0.05), and the mean value of cross-sectional area of bilateral psoas major muscle was decreased on the same level (P<0.05). No significant difference in the mean value of cross-sectional area of left and right erector spinae was detected on the same level (P>0.05). Results indicated that the changes in cross-sectional area of paraspinal muscle could induce degenerative lumbar spondylolisthesis, and could be considered as a diagnostic standard for degenerative lumbar spondylolisthesis.

Objective To investigate the rationality and prognostic value of the Criteria for clinical staging of esophageal cancer treated by non-surgical methods (Draft) by comparison with the criteria for postoperative pathological staging.Methods A retrospective analysis was performed on the clinical data of 162 patients with esophageal cancer who underwent radical resection at the Second Hospital of Soochow University from December 2008 to July 2012.Preoperative clinical staging and postoperative pathological staging were performed,and the Kappa statistic was used to evaluate the agreement between the two staging systems.The Kaplan-Meier method was used to calculate overall survival (OS) rates,and the log-rank test was used for survival difference analysis and univariate analysis.Results The coincidence rates of T,N,and TNM stages were 67.9％,57.4％,and 67.9％,respectively,and the agreements were moderate,poor,and moderate,with Kappa values of 0.544,0.302,and 0.509.The follow-up rate was 93.2％.The 1-,2-,and 3-year sample sizes were 127,66 and 27,respectively.The 1-,2-,and 3-year OS rates were 82.6％,56.2％,and 37.7％,respectively.There were no significant differences in OS between patients with preoperative T1 and T2 stages and between patients with preoperative N0 and N1 stages (P =0.086,0.101),but significant differences were observed between patients with different T stages,N stages,or TNM stages (P =0.000-0.028).This was in line with the prognostic results based on the postoperative pathological staging.Conclusions The clinical staging criteria (draft) have moderate agreement with the criteria for postoperative pathological staging and have good prognostic value,but they require further refinement and improvement.

Objective To study anti-tumor effects on human ovarian cancer xenografted tumors in mice by constructing adenoviral expression vector containing canstatin gene and hTERT gene core promoter (AdhTERT-Can). Methods AdhTERT-Can vector was constructed and identified by means of enzyme cutting, electrophoresis and sequencing. Then, transfected into HO8910PM cells by means of lipofectamine and confirmed by green fluorescence protein(GFP) expression under laser confocus microscope. The mRNA expression of canstatin gene was tested by RT-PCR. Human ovarian cancer xenografted tumor models in nude mice were established and randomly divided into AdhTERT-Can group received viral supematant solution of AdhTERT-Can by tail vein injection, Ad-Can groups received viral supernatant solution of Ad-Can by tail vein injection, and control groups received phosphate buffer solution (PBS). The volume of tumors were measured and compared in each group to evaluated the anti-tumor efficacy. Results All the constructed vectors of AdhTERT-Can were verified by enzymed digestion. There were green fluorescence from 60% of HO8910PM cells transfected by AdhTERT-Can under laser confocus microscope, and the mRNA expression of canstafin gene in HO8910PM cells were also verified by RT-PCR The growth of tumor in AdhTERT-Can group was significantly inhibited compared with those in Ad-Can groups and control groups from the 8th day (P <0.01 ). However, there were not significant difference between Ad-Can group and PBS group (P> 0.05). On the 30th day, the tumors showed liquefaction necrosis and cystic degeneration in each group, especially in AdhTERT-Can group. Conclusions The recombinant adenovirus vector of AdhTERT-Can has been constructed successfully and could steady express in ovarian cancer cell lines HO8910PM. The results shown that it could inhibit significantly the growth of human ovarian cancer xenografted tumors in mice and shown to be the target of gene therapy.

Objective: To investigate the rule of the alignment of the major and minor segments of the maxillary alvedar bone in infants with comp lete unilateral cleft palate (CUCP).Methods: 30 models of CUCP were used in the study,Markers on the model were designed.The lenth,width, midline and the malposition of minor and major segments on both sides of the cle ft were measured and analyzed.The models of 9 normal infants were employed as th e controls.Results: In CUCP group,the width of alvelar arc h was found significantly larger ( P

Objective To investigate the proliferation and the change of antigen on the cord blood CIK cells. Methods Cord blood CIK cells were generated by culture cord blood mononuclear cells in the presence of ?-IFN on day 0 and rhIL-2, antiCD3 MCAb on day 1. CIK cultures were analysed on different time point by FACS(Fluorescence activated cell sorter). The killing efficacy in tumor cell lines and primary leukemia cells were detected by MTT assay. Results After 2~3 weeks of culturing cord blood CIK cells expanded about 64.14?16 folds and CD+3 CD+56 cells which were the major cytolytic effector in CIK cells proliferated about 900 folds. Cord blood CIK cells have great cytotoxity against tumor cell lines as well as primary leukemia cells. Conclusions Cord blood MNCs can be cultured to CIK cells and the times of expansion are very high. Cord Blood CIK cells are highly efficient cytolytic effector cells.

To study the mechanism and influence of pneumonia on myocardium in D-galactose(D-gal) treated mice, 36 normal male NIH mice were randomly selected and pretreated with D-gal, and then they were divided into three groups, namely control group, the first day after pneumonia group, and the third day after pneumonia group. Another group of 36 mice were pretreated with normal saline to form a general control group. The result showed that in the D-gal prtreated pneumonia mice, the degeneration of myocardial mitochondria was more pronounced, the content of MDA in myocardium was increased, and the activity of SOD and the content of ATP in the myocardium were reduced. There was a positive correlation between the content of ATP and the activity of SOD, and there was a negative correlation between the content of ATP and MDA in the myocardium. These results suggusted that the myocardial lipid peroxidation in the D-gal treated mice increased the susceptibility to the damaging factors, such as pneumonia.