Polymerase chain reaction (PCR) technique for the diagnosis of hematological disease brought about revolutionary progress. It has become indispensable and basic technology for genotyping of acute and chronic leukemia and diagnosis of myeloproliferative disorders, thalassemia, hemophilia and other genetic diseases. Based on the PCR technology, multiple nested-PCR, real-time fluorescence quantitative PCR (Q-PCR), denaturing high pressure liquid chromatography (DHPLC), capillary electrophoresis, the molecular hybridization and directly sequencing technique have been developed. The multip]e nested-PCR can be used to examine a variety of leukemia genes and confirm the type of leukemia. The application of Q-PCR has effectively solved the problem of pollution of the PCR and it can be used to monitor minimal residual disease of leukemia. Capillary electrophoresis is more suitable for gene fragment length change. DHPLC can be applied for high-throughput screening of gene mutation. Sequencing is a reliable method and it has been used for routine diagnosis.

Immunophenotyping with flow cytometry can clearly identify multiple myeloma cells and monitor minimal residual disease by using the characteristic of light chain restriction in plasma cells and some cell surface markers( eg CD138, CD38, CD56, CD117 ). This technology may provide important evidence in the diagnosis of multiple myeloma.

Application of imatinib has made a lot of chronic myeloid leukemia (CML) patients longterm survival,but the CML BCR/ABL1 kinase domain mutations (KDM) will result in drug resistance of imatinib.There are many KDM types.The different types appear randomly.The leukemic clone with different KDM characteristics has a certain evolution.In the period of imatinib treatment,a higher degree of drug resistance mutations in cells likely to develop as the dominant clone.It is suggested that the treatment of imatinib-resistant CML in addition to efforts to develop next-generation tyrosine kinase inhibitors outside,but also consider the use of traditional medicine to help cure.

With the fierce competition of higher education market,the public university and college face the pressure from mana-gement toward economic operation.This article tries to make some study on what the economic operation innovation of the public higher education institutions is,to make a synopsis analysis on disadvantageous factors and advantageous factors,and to put forward some suggestions on its promising system.

Thirty-four patients with acne vulgaris were treated with pricking and ear-pressure. Acupoints were Baihui (GV 20), Dazhui (GV 14), Taodao (GV 13), Shenzhu (GV 12), Shendao (GV 11), Lingtai (GV 10), Feishu (BL 13), and Sanyinjiao (SP 6), for priking. Lung (MA-IC1), Stomach (MA-IC),Endocrine (MA-IC3), Subcortex (MA-AT1), EarShenmen (MA-TF1) and Cheek (MA-L) were applied for ear-pressure. After 4 courses' treatments, 33 cases obtained effectiveness and 1 case ineffectiveness.

OBJECTIVEThis study aims to compare the fracture resistance of teeth restored with different post and core systems by cyclic loading test and to predict long-term effects.

METHODSA total of 15 recently extracted mandibular premolars without crowns were endodontically treated. The teeth were randomly divided into three groups with five teeth in each group. Group A, teeth restored with cast metal post and core. Group B, teeth with fiber post and resin core. Group C, teeth with resin filling. All of the teeth were restored with full-cast metal crowns. The specimens were exposed to 300,000 cyclic loadings in a cyclic loading machine and 1,000 thermal cycles between 5 and 55 °C. All of the samples were loaded in AG-IS mechanical testing machine with a compressive load until fracture occurred. The fracture load and the fracture mode of each tooth were recorded.

RESULTSThe fracture resistance in group B and C were higher than that of the cast metal post and core restoration (P < 0.05). Repairable fractures were observed in group B and C, whereas unrestorable and catastrophic fractures were found in group A.

CONCLUSIONFiber post and resin filling is a good choice to restore endodontically treated teeth.

Objective: To investigate the effect of oat on blood glucose、insulin and serum lipid in aged patients with type-2 diabetes mellitus.Methods: Blood glucose(FBG,PBG),insulin(F-INS,P-INS) and serum lipid(TG,TC) were determined in eighty aged patients with type-2 diabetes mellitus before and after 50-100 g oat intake. Results: FBG,PBG and P-INS were decreased significantly(P

In this review,the pathogenesis,clinical presentations,diagnosis of occupational asthma(OA)are discussed.The agents that cause OA are more than 300 kinds of high and low molecular-weight substances.The diagnosis of OA is generally established on the basis of a suggestive occupational history,symptoms,abnormal lung function and lab examination.Specific inhalation of bronchial challenge(SIC)comes closest to a gold standard test for some agents causing(sensitizer-induced)OA.

Objective To investigate the effect of the selective cyclooxygenase-2 inhibitor celecoxib on the expression of PGE2 and VEGF induced by IL-1? in A549 cell lines.Methods A549 cells were incubated with IL-1? in the presence or absence of celecoxib.After indicated incubation time,cells were harvested to detect VEGFmRNA by reverse transcription-polymerase chain rection(RT-PCR),the medium was collected to determine the concentration of PGE2 and VEGF by enzyme-linked immunosorbent assay(ELISA).Results After Celecoxib intervention,the formation of PGE2 and VEGF expression decreased more than control group(IL-1? induced),the difference being significance(P

Objective To compare the operative efficacy of low collar incision and L-shaped incision plus cervical plexus preser-ving lymphadenectomy in treating papillary thyroid carcinoma.Methods 9 3 inpatients with papillary thyroid carcinoma were ran-domly divided into the low collar incision group (n=37)and the L-shaped incision group(n=56)according to the random number table.The low collar incision group underwent the low collar type cervical plexus preserving lymphadenectomy,while the L-shaped incision group received the L-incision cervical plexus preserving lymphadenectomy.The postoperative 24-month follow up was per-formed.Then the clinical efficacy and the life quality of the patient were evaluated and compared between the two groups.Results (1)1 case of lymph node recurrence in the district Ⅱ after 20 months was found in the low collar incision group,1 case of lymph node recurrence in the district Ⅰ after 12 months and 1 case of lymph node recurrence in the districtⅣ after 22 months was found in the L-shaped incision group;no case of distant metastasis in the low collar incision group occurred during the follow up period, but 1 case of pulmonary metastasis in postoperative 5 months and 1 case of bone metastasis in postoperative 24 months occurred in the L-shpaed incision group.The differences in the occurrence rates of postoperative recurrence and metastasis had no statistical sig-nificance (2.70%vs.7.14%).(2)The scores of neck shoulder pain and numbness,incision appearance satisfaction degree in the low collar incision group were significantly higher than those of the L-shaped incision group (P<0.05).Conclusion Compared wtih L-shaped incision cervical lymphadenectomy,low collar incision cervical plexus preserving cervical lymphadenectomy has simi-lar high clinical efficacy,can significantly reduce neck shoulder pain and numbness,meet aesthetic requirements and especially is suitable for the patients with higher demands of the neck function preserving and the neck appearance.