In order to enhance the quality of teaching,cultivate students’thought-habit ofdynamically development and mutuality,and to improve their ability of analysis and integration towards complex issues,we experimentally applied Philosophic Mode of the Bicyclic and Tricyclic mutual crosses put forward by Professor Feng Zhiqiang of Luzhou Medical College to physiological experimental teaching,and got good teaching effect.

Objective:To observe the toxic effects of artemisia aqueous extract on the ability of reproduction in male mice.Methods:Mice were randomly divided into 0,20,100 and 500 mg/kg dose groups and administered intraperitoneally for 60 days then mated and exeduted. The coefficient of testicle and epididymis,fertility index,sperm,the percentage of motile sperm,deformity of sperm and histopathological changes of testicle were detected.Results:The concentrations of artemisia aqueous extract used in this study have no significant influence on the aforementioned parameters.Conclusion:Reproductive functions of male mice does not obviously impaired by artemisia aqueous extract.

Objective: To investigate the effects of Zhu Yun Pill(ZYP) in the treatment of infertility due to luteal phase defect(LPD).Methods: 163 infertility patients due to LPD were randomly divided into three groups in terms of ZYP group,progesterone group and combination group(ZYP and progesterone).Then the therapeutic efficacy was judged by the pregnancy rate(PR),endometrial biopsy,the level of serum hormone and basal body temperature before and after the treatment.Result: 40 women were pregnant in ZYP group(cure rate,33.33%),8 in the progesterone group(cure rate,26.67%) and 9 in the combination group(cure rate, 69.23%).The symptomatic amelioration and total effective rate of ZYP group were obviously higher than single exogenous progesterone group(P

Objective To explore the clinical effect of laparoscopy combined with deep hyperthermia in the treatment of pelvic abscess.Methods Clinical data of 56 patients with pelvic abscess treated by laparoscopy combined with deep hyperthermia were retrospectively analyzed.Results 56 cases were completed in laparoscopic surgery,before surgery,after diagnostic accordance rate was 76.8%,average operation time was 51 minutes,postoperative average hospitalization was 5.1 days.After operation they were performed in vitro hyperthermia to increase the efficacy. Patients were followed up for 6 months,no recurrence.32 patients with fertility requirements of the fallopian tube,1 to 3 months after the operation of bilateral fallopian tube radiography,20 cases of patency,8 cases of tubal patency and poor.Conclusion Laparoscopy combined with deep hyperthermia in vitro is an ideal and safe treatment,which is conducive to protect the patients'reproductive function,especially for young patients with no pregnancy.

Human bocavirus ; genetics ; isolation & purification ; metabolism ; pathogenicity ; Humans ; Neonatology ; Research Report

Objective To investigate the efficacy of injecting insulin in family diabetes mellitus in Xiaofeng town community in Huzhou area and the problems of injection technique.Methods From February 2015 to December 2016, 94 patients with diabetes were injected with insulin in Xiaofeng town community in Huzhou area.From February 2015 to 3016, the fasting blood glucose(FPG), 2 h postprandial blood glucose 2hPG) and glycosylated hemoglobin(HbA1c), and to investigate the problems in the process of injecting insulin by questionnaire.Results After 3 months of injection, FPG, 2hPG and HbA1c were decreased in all patients(P<0.05).71 cases(75.53) were regularly rotated in the injection of insulin and 82 cases were alcoholic(81.9%), the use of 5 mm needle 86 cases(91.49%)[our hospital only 5 mm and 8 mm Needle, jurisdiction of the surrounding hospital needle is 6 mm.(76.20%), 12 cases(12.77%) of insulin needle, and 2 to 3 days of insulin needle(72.60%) were injected in the subcutaneous 10s(71.28%) after injection, 16 cases(17.2%) were replaced, 10 cases(48.94%) were replaced on the 10th day, and 11 cases(11.70%) were replaced when the insulin was replaced.9 cases(9.57%) were replaced after the pain.Conclusion Diabetic patients have definite therapeutic effect on insulin injection in family, but there are some defects in injection technique and injection hygiene.Therefore, health care workers need to strengthen their knowledge of injection of insulin injections in families.

Adult ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Heart Neoplasms ; pathology ; Humans ; Myxoma ; pathology ; Ovarian Neoplasms ; secondary ; surgery ; Pulmonary Veins ; Sarcoma ; diagnostic imaging ; metabolism ; pathology ; secondary ; surgery ; Tomography, X-Ray Computed ; Vascular Neoplasms ; diagnostic imaging ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; adverse effects ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; pathology ; Cisplatin ; administration & dosage ; adverse effects ; Deoxycytidine ; administration & dosage ; adverse effects ; analogs & derivatives ; Drug Chronotherapy ; Female ; Humans ; Leukopenia ; chemically induced ; Lung Neoplasms ; drug therapy ; pathology ; Male ; Middle Aged ; Nausea ; chemically induced ; Neoplasm Staging ; Neutropenia ; chemically induced ; Remission Induction ; Taxoids ; administration & dosage ; adverse effects

Background Studies showed that microRNA (miR)-375 suppresses the growth,apoptosis,migration and adhesion of tumor cells,and it plays a regulation to the changes of vascular endothelial growth factor (VEGF) in tumor tissue to arrest neovascularization.However,whether miR-375 intervenes the formation of new blood vessel in eyes is unelucidated.Objective This study was to explore the effects of miR-375 on human retinal capillary endothelial cells (HRCECs) function induced by hypoxia.Methods HRCECs were cultured using IMDM and divided into normal control group,CoCl2 model group,CoCl2 +miR-375 mimic group,CoCl2 +miR-375 mimic control group,CoCl2+miR-375 inhibitor group and CoCl2 +miR-375 inhibitor control group,and hypoxia cell models were created by adding 200 μmol/L CoCl2.MiR-375 and frizzled 4 (FZD4) small interfering RNA (siRNA) were transfected into the cells by 50 nmol/L miRNA lipidosome for 48 hours.The proliferation of the cells was detected by MTT assay;migrated number of the cells was examined by Transwell chamber assay;ELISA was employed to detect the concentrations of VEGF and VE-cadherin in the medium;the expression of β-catenin,cyclinD1,matrix metalloproteinase-2 (MMP2) and VEGF proteins were analyzed by Western blot;tube length of vessel formation was evaluated by Matrigel assay.Cultured cells were divided into normal control group,CoCl2 model group,CoCl2 +mock group and CoCl2 + FZD4 siRNA group,the relative expression of FZD4,a miR-375 targeted gene,was detected by luciferase reporter.Results The relative expression of miR-375 mRNA was significantly increased in the CoCl2 +miR-375 mimic group compared with the CoCl2 + miR-375 mimic control group and reduced in the CoCo2 + miR-375 inhibitor group compared with the CoCo2 + miR-375 inhibitor control group (t =-19.237,8.764,both at P＜0.01),with a higher transfected efficacy for miR-375.The cell proliferative fold,migrated cell number,VEGF and VE-Cadherin contents in the medium and the tube length were significantly different among the CoCl2 model group,CoCl2 +miR-375 mimic group,CoCl2 +miR-375 mimic control group,CoCl2 +miR-375 inhibitor group and CoCl2 +miR-375 inhibitor control group (F =24.324,26.776,14.113,19.225,15.040,all at P＜0.001),and those in the CoCl2 +miR-375 mimic group were evidently reduced in the CoCl2 +miR-375 mimic group compared with the CoCl2 +miR-375 mimic control group,while those in the CoCl2 +miR-375 inhibitor group were considerably elevated in comparison with the CoCl2 +miR-375 inhibitor control group (all at P＜0.01).The expressions of β3-catenin,cyclinD1,MMP2 and VEGF protein were significantly different among the normal control group,CoCl2 model group,CoCl2 +miR-375 mimic group,CoCl2+miR-375 mimic control group,CoCl2 +miR-375 inhibitor group and CoCl2 +miR-375 inhibitor control group (F=11.753,13.283,16.770,10.334,all at P＜0.001).In addition,the cell proliferative fold,migrated cell number and the tube length were significantly increased in the CoCl2 model group and CoCl2+mock group,and those in the CoCl2+FZD4 siRNA group were decreased in comparison with the CoCl2 +mock group (all at P＜0.05).Conclusions MiR-375 inhibits the growth,migration and tube formation ability of HRCECs in hypoxic status probably by regulating the activation of Wnt pathway via directly targeting FZD4.

AIM:To find out the mean corneal spherical aberration and its changes with age in Kaifeng population.
METHODS: A total of 466 eyes of 427 Kaifeng subjects with cataract, whose age ranged from 52 to 75 years old, were divided into three groups according to the age. All the subjects were included in measuring K-readings of cornea, the corneal anterior and posterior surfaces of the spherical aberration and the total corneal spherical aberration using Pentacam(Oculus, Germany) at the 6-mm optical zone.
RESULTS:The mean corneal spherical aberration( CSA) of the whole groups was 0. 339±0. 153μm. Patients from 50 to 60 years old had CSA of 0. 322±0. 137μm, 0. 326±0.157μm was the CSA of the patients from 60 to 70 years old, while those from 70 to 80 years old have a CSA of 0.401 ± 0. 182μm. The corneal K - readings had no correlation with the total corneal spherical aberration ( r=0. 034, P>0. 05), anterior surfaces CSA, posterior surfaces and total CSA were positively correlated with age ( r =0.253, 0. 327, 0. 233, all P<0. 01).
CONCLUSION:CSA is varied among the population and CSA of elderly in Kaifeng area are slightly higher than white people, and positively correlated with age.