Objective To explore the diagnostic value of the quantitative detection of serum miRNA-193a in pulmonary adenocarcinoma.Methods 40 pulmonary adenocarcinoma patients and 40 healthy people were recruited to receive quantitative detection of serum miRNA-193a and CEA.And then the relationship between the concentration of miRNA-193a and CEA was analyze by spearman correlation analysis.12 of the 40 patients underwent operation and the different levels of miRNA-193a expression among pulmonary adenocarcinoma tissues,adjacent tissues and normal tissues was detected by qRT-PCR.The different miRNA-193a levels were compared among groups of by t-test.The diagnostic performance of miRNA-193a was evaluated with receiver operating characteristic curves(ROC) and the area under the curve (AUC) and its 95%CI were calculated.Results The relative expressions of miRNA-193a in pulmonary adenocarcinoma tissues,adjacent tissues and normal tissues were 1.07±0.16;0.80±0.10;0.11±0.07.miRNA-193a expression in pulmonary adenocarcinoma tissues was significantly lower than that in other two groups(P<0.05).The expression of serum miRNA-193a in pulmonary adenocarcinoma group was significantly lower than that in healthy group(P<0.05).There was no correlation between miRNA-193a and CEA(r=0.079,P=0.618).The detection of miRNA-193a yielded a ROC AUC of 0.829(0.741-0.917) in discriminating pulmonary adenocarcinoma from healthy group.Conclusion The relative expression of miRNA-193a in pulmonary adenocarcinoma was significantly lower than that in healthy controls no matter in tissue or serum,serum miRNA-193a may prove to be a non-invasive biomarker for the auxiliary diagnosis of pulmonary adenocarcinoma.

Percutaneous transluminal coronary angioplasty (PTCA) was done successfully in 33 patients,6 of them were associated with 8 acute coronary events. Radioimmunoassay was used for the determination of plasmaET,AII and CGRP levels. The samples were drawn successively from femoral artery just before PTCA and 0min, 15min,1h,3h,24h after the final balloon inflation. The results showed that the levels of plasma AII were higher in the complication group than those of noncomplication group before PTCA and 0min, 24h after PTCA. The differences in plasma ET levels before and after PTCA between the complication group and the non-complication group were not significant. The level of plasma CGRP was decreased at 15min and returned to basal level at 1h after PTCA in the complication group.In the patients without complication,there were no significant differences in their plasma CGRP levels before and after PTCA. All + ET/CGRP in the complication group raised more rapidly,recovered later and the peak appeared earlier than that of non-complication group. For a better understanding pathophysiologic meaning of the above changes,further studies are needed.

There were no data concerning restenosis rate after percutaneous transluminal coronary angio-plasty available in China so far. Our study was focused on clinical and angiographic follow-up and tried to reveal the predictive value for angiographic restenosis using noninvasive tests among 592 patients whose 841 coronary arteries were dilated in our department from Dec. 1987 to Dec. 1994.This patient group consisted of 503 males and 89 females had average age of 58. 4 ? 9. 1. The overall success rate was 93. 3% and acute vessel complications was 4. 1%. After successful procedures of PTCA,the patients were followed up with recurrence of angina, Holler ECG, submaximal excercise ECG and 99mTc-MIBI exercise scintigraphy regularly. 62 of these patients underwent coronary angiographic follow-up from 4-24 months (average time: 10. 4 ? 7. 6 months) after PTCA. We found 35 patients with angiographic restenosis and 27 patients without. Thus, we divided the patients into restenosis group and non-restenosis group according to the angiographic results. Subsequently, we oberserved whether the symptom and noninvasive tests were valuable in prediction of restenosis. The result showed that recurrence of angina, Holter ECG, submaximal exercise ECG and 99mTc-MIBI scintigraphy were useful in detecting restenosis with their specificity and sensitivity 75.7% and 72.0%, 71. 4% and 86. 7%, 66. 7% and 73. 1% ,76. 5% and 83. 3 %, respectively. Moreover, If any two of above four ischemic indexes were combined together to detect restenosis,the specificity and sensitivity were significantly enhanced (85. 2% and 92. 9%). It is concluded that recurrence of angina and the noninvasive tests were valuable in predicting restenosis. The combination of these ischemic indexes would greatly improve their predictive value.

Objective: To explore the express of type A behavior in families with and without history of hypertension Method: 178 members of 40 families with history of hypertension and 27 families without completed type A behavior questionnaire Result: Type A behavior was not associated with hypertension 35 2% children of both parents with type A was type A, 34 4% children of both parents with type B was type B Children of one parent with type A were more commonly manifested as type B Conclusion: Type A behavior has no association with hypertension Behavior pattern itself has family constellation

Objective To determine whether the raised serum uric acid(UA) level is the tindependent risk factor Hypertension.Methods A retrospective study was performed including 128 Hypertension patients(male 99,female 29) with average age of(64.49?11.03) and 87 norma Hypertension patients(male 53,female 34) with average age of(61.25?10.98) .A fasting blood samp les were collected for measurement of serum UA,blood cholesterol,and so on. And all patients were carefully inquired for their history including smoking、forcoronary artery disease and DM.Results Althoughraised serum UA was observed in Hypertension patients(496.31?42.33) mmol/L vs(356.08?50.68) mmol/L(P

Objective:To repair unilateral incomplete cleft lip with Noordhoof's incomplete cheiloplasty. To analyze the properties of the technique and to emphasize the key points of the operation design and manipulation. Methods:52 cases of unilateral incomplete cleft lip were received Noordhoff treatment. The characterization of operative method, results and attention were analyzed. Results: 51 cases are totally healed successfully except 1 case is healed successfully by re-operation for losing balance onto the floor. Conclusion: With Noordhoof's surgical technique for unilateral incomplete cleft lip, parenchyma of the lip is utilized mostly, the nosewing outboard incision is avoided. The shape of vermilion is more satisfactory.

Objective To evaluate the laparoscopic ovarian cystectomy of benign lesions of security,superiority.Methods Laparoscopic ovarian cystectomy of benign lesions of security,superiority.Results The operation time of laparoscopic group (40.5 ± 5.3) min,significantly shorter than the control group (60.7 ± 6.8) min (t =-14.815,P =0.000) ; Bleeding (55.1 ± 9.8)ml was significantly less than that in the control group (90.0 ±15.0) ml (t =-18.275,P =0.001) ; Anus exhaust time (13.5 ± 8.3) hour was significantly shorter than that in control group (24.5 ± 9.6) hour (t =-4.351,P =0.000).The average postoperative hospital stay was (3.5 ±1.3) days was significantly shorter than that in control group (5 ± 2) days (t =-2.158,P =0.000).Postoperative morbidity was not statistically different from controls (t =0,5,P =0.053).Postoperative pain was significantly lower than that in control group (x2 =8.525,P =0.002).Conversion to open operation in laparoscopic group:2 cases,postoperative analgesic drugs were not needed,the postoperative 12h after catheter removal started eating and ambulation,postoperative shoulder pain in 2 cases ; open operation group after all need to use analgesics,postoperative 24h after catheter removal started eating and ambulation,abdominal incision fat liquefaction and delayed healing in 4 cases.The two groups had no visceral injury,postoperative bleeding,and chemical peritonitis.Conclusion Laparoscopy in the treatment of benign ovarian tumor is safe,less bleeding,rapid recovery,fewer complications and other characteristics.

Objective To investigate the effect of heparin on the apoptosis and proliferation of human myeloid leukemia cell line K562 induced by vincristine.Methods K562 cells were pretreated by heparin for 1h,then cultured with 0.05 mg/L vincristine in 37℃ 5% CO2 for 24 h.Apoptosis of KS62 cells was evaluated by Hoechst 33342 staining,flow cytometer and DNA agarose gel electrophoresis after culture for 24 hours.The effect of heparin on KS62 cell proliferation and toxicitv was determined by Trypan blue staining and MTT assay.Results In the apeptosis induced group,the apeptosis rate was 40.10% dected by Hoechst 33342 fluorescence staining.The hepafin in different concentrations was found to be able to inhibit the apoptosis of K562 cells triggered by vincristine and the apoptosis rate was 32.47%,29.7%,25.5%,19.53% in the heparin groups of 25,50,100,200 U/ml,respectively.The apeptosis rate was significantly lower in the apeptosis induced group than in the heparin groups of 25,50,100,200 U/ml(P＜0.05).The typical DNA ladder could be found in the apoptosis-induced group,and the DNA ladder gradually disappeared along with the increase of heparin(5～200 U/ml).The sub-G1 peak of K562 cells could be found in the induced group by FACS and the apoptosis rate was 21.61%.In the heparin groups of 25,50,100,200 U/ml,the sub-G1 Peak of K562 cells gradually dropped and the apoptosis rate was 13.64%,11.75%,8.59%,6.03%(P＜0.05),respectively.After K562 cells were incubated with different hepafin concentrations(5～200 U/ml)for 24 hours,there was no difference compared with the normal control group in both the total live cell numbers and the cell proliferation rate measured by trypan blue staining and MTT assay(P＞0.05).Conclusion The results suggested that heparin had no influence on KS62 cell toxicity and proliferation,but may inhibit the apoptosis of KS62 cells induced by vincristine.

Objective To investigate the relationship between hepatitis B virus (HBV) genotype and liver function. Methods The method of microboard nucleate molecular hybridization was employed to detect the genotype in 93 HBV patients of different clinical types and the function of liver. Results Among the 93 HBV patients of different clinical types, there were 24 cases of genotype B (25.81%), 59 cases of genotype C (63.44%), 5 cases of genotype D (5.38%), and 5 cases of mixed type (3 cases of B/D, 2 cases of C/D, 5.38%). Therefore, genotype C took up the largest proportion, followed by genotype B, and then D and mixed genotypes, but there was no genotype A, E or F. The detection rate of genotype C increased according to the sequence of chronic hepatitis B, subacute severe hepatitis and hepatocirrhosis while the detection rate of genotype B decreased gradually. However, the detection rate of genotype C in hepatocellar carcinoma did not rise correspondingly. The levels of ALT, AST and TBIL of genotype C were higher than those of genotype B, but the level of ALB in genotype C was lower than that of genotype B. None of the differences had significance. Conclusion Most of HBV genotypes in Xi'an were C, some of them were B, D and mixed genotypes, but no genotype A, E or F was detected. Except hepatocellar carcinoma, the detection rate of genotype C rose according to the severity of clinical type.