Objective:To eliminate the interference of matrix in urea cream on the microbial limit test. Methods: A certain a-mount of magnesium sulfate was added to the test solution followed by stirring and placing sometime to make the cream layering, and the water layer was used for the microbial limit detection. Results:After magnesium sulfate was added to urea cream, microbial limit test was not affected by the matrix. The average recovery of Escherichia oli, Staphylococcus aureus, Bacillus subtilis, Candida albicans and Aspergillus niger was 96. 00%, 97. 00%, 100. 00%, 99. 00% and 101. 00%, respectively. The negative and positive results of Psudomonas aeruginosa and Staphylococcus auress were reliable. Conclusion:In the detection of microbial limit for urea cream, mag-nesium sulfate can be used as a good auxiliary agent to make the detection method more convenient and reliable.

Objective To explore the relation between traumatic disseminated intravascular coagulation (DIC) and the level of plasma thrombomodulin (TM) in severe multiple-injury patients. Methods Sixty-six multiple-injury patients were divided into minor-injury group (ISS

To evaluate the role of nitric oxide in local function of endometrium, the pattern of expression of endothelial and inducible nitric oxide synthases in the human endometrium at proliferative and secretory phases and decidua was studied by using immunocytochemistry. Results showed: (1) At the proliferative phase, the eNOS immunostaining was confined to vascular endothelial, whereas the iNOS was not detected in any composition of endometrium. (2) At the secretory phase, surface epithelium and grandular epithelium showed positive staining for both eNOS and iNOS. The stroma remained uniformly negative throughout the menstrual cycle. (3) In the decidua, the expression of both isoforms was increased, while moderate iNOS immunoreactivity was observed in decidualized stromal cells. It was demonstrated that the expression of eNOS and iNOS was elevated at the secretory phase and in decidua, indicating the increased production of NO at these phases. The increasing of NO might take part in implantation through dilating the vessels and relaxing the smooth muscles and in menstration through promoting apoptosis.

To evaluate the role of nitric oxide in the embryo implantation in mice, three experiments were carried out using the mice implantation model. In the experimentⅠand experimentⅡ, NG-nitro-L-arginine methyl ester (L-NAME), the non-specific NOS inhibitor, was administered intraperitoneally at doses of 0.5 mg-5 mg on the day 3 of pregnancy and at dose of 3 mg on different days of pregnancy (day 1-6) . In the experiment Ⅲ, L-aragnine, a donor of NO, was co-administered with L-NAME to evaluate the effect of L-aragnine on L-NAME, and the embryo development was assessed. In all these three experiments, the endometrium was histologically examined. Results showed compared with the control groups intraperitoneal administration of a dose of L-NAME between 1 and 5 mg on the day 3 of pregnancy led to the decrease in the number of implantation sites (P<0.05), and 4 to 5 mg of L-NAME caused inhibition of implantation completely. L-NAME resulted in failure of pregnancy when administrated at 3 mg between day 3 and 5 of pregnancy. The characteristic vascular permeability changes and decidualization in the endometrium were significantly attenuated and embryo growth was retarded. The L-NAME-mediated effects were significantly reversed when L-aragnine was co-administered with L-NAME. This study demonstrated that NO promoted the implantation in mice through regulating the permeability and decidulization of endometrium and the development of embryo.

Background and purpose:Fallopian tube carcinosarcoma is a rare malignant tumor that is prone to metastasis and recurrence. Patients with the malignancy often have poor prognosis. This paper retrospectively analyzed the clinical manifestation, pathology, treatment and prognosis of fallopian tube carcinosarcoma.Methods:The clinical manifestations, pathological features, treatment and survival time of 7 cases of fallopian tube carcinosarcoma were retrospectively analyzed.Results:The most common symptoms of 7 cases of fallopian tube carcinosarcoma were irregular vaginal bleeding (4/7, 57.1%). Among 7 cases, one case was stageⅠa, 1 stageⅡa, 1 stageⅡb, 1 stageⅢb, and 3 stageⅢc. Four cases (57.1%) were heterologous and found to have cartilage components whereas 3 cases (42.9%) were homologous. Seven cases were performed with tumor volume reduction surgery, and 6 cases with chemotherapy, while no one received radiotherapy. Five patients survived and 2 were dead. The survival time of 3 patients was more than 5 years. The longest survival time to the end of the follow-up was 114 months.Conclusion:The pathological examination is the diagnostic means of fallopian tube carcinosarcoma. The prognosis of the patients with the pathological characteristics of the homologous is better than that of the heterologous. Satisfactory reduction of tumor, postoperative chemotherapy with adequate TP regimen, combined with systemic chemotherapy and intraperitoneal chemotherapy may be an effective treatment for fallopian tube carcinosarcoma.

Purpose: To observe the clinical therapeutic effect in the treatment of enuresis by ginger-partitioned moxibustion plus cupping therapy. Methods: Ginger-par -titioned moxibustion was applied on Guanyuan (CV 4)and bilateral Zusanli (ST 36) and cupping therapy was applied on Shenque (CV 8) in the treatment of 82 cases of enuresis, in comparison with 76 cases treated by Chinese herbal medicine. Results: The effective rate was 84.1% in the treatment by ginger-partitioned moxibustion plus cupping therapy and was 64.5% in the treatment by Chinese herbal medicine. Conclusion: Ginger-partitio -ned moxibustion plus cupping therapy was better than Chinese herbal medicine in the treatment of enuresis (P ＜0.05).

In the treatment of 80 cases of the patients with deficient and cold pattern in the spleen and stomach by external application of herbal paste on the acupoints, the results showed remarkable effect in 52 cases, improvement in 24 cases, failure in 4 cases and the total effective rate in 95.0%. Remarkable therapeutic effects were achieved in three different diseases of same pattern. External application of herbal paste on the acupoints is definitely effective for stomachache in deficient and cold pattern of the spleen and stomach.

Making dental impressions and models is a part of daily routine work for dental nurses in the orthodontic department in China. This paper analyzes the common problems encountered in the process of making dental impressions and models, and emphasizes the importance of quality control and supervision during this process. A method of combining real-time monitoring and periodic inspection based on nurse-doctor cooperation was put forward to ensure the quality of dental impressions and models. Firstly, the nurses should be well trained with the instruction of doctors before on duty; Secondly, the head nurse should be responsible for implementing real-time monitoring and periodic inspections to find the problems and take measures in time. Also, the doctors should check the dental impressions and models in time, especially for those models with special demands, and help the nurses to improve their skills constantly.

BACKGROUND: Tissue engineering has a promising prospect in corneal transplantation. Scaffolds are always restricting the development of tissue-engineered cornea. OBJECTIVE: To analyze application of different seed cells and scaffolds, and to summarize the progress of tissue-engineered cornea in recent years.METHODS: First author searched literature from CNKI (2000/2010-10) and PubMed database (2000/2010-10). The key words are tissue engineering, corneal transplantation in Chinese or English. A total of 223 literatures were seized by computers, according to the inclusion criteria, papers concerning the advance, application and reconstruction of tissue-engineered cornea were analyzed. Finally, 33 papers were included for further analysis. The present study was to analyze the seed cells, scaffolds, organ building and clinical applications of tissue-engineered cornea and investigate the development direction in the future. RESULTS AND CONCLUSION: The results show that seed cells and scaffolds are the focus of the studies now. Corneal transplantation has a high success rate in organ transplantation since the immune privilege of eye and avascular cornea, and it will be able to be a tissue engineering organ that can be largely built, easy to transplant. Reconstruction of cornea has reached first base, but every kind of scaffold has certain drawbacks, so the next goal is to find an ideal scaffold material.

Objective To explore the clinical application of function-preserving pancreatic surgery for treating pancreatic neuroendocrine neoplasms (pNENs).Methods Twenty-eight patients with pNENs treated by the function-preserving pancreatic surgery at Anhui Province Hospital from January 2002 to December 2015 were analyzed retrospectively.Results Among the 28 patients, enucleation surgery was performed in 18 cases, distal pancreatectomy was performed in 8 cases, and middle pancreatectomy was performed in 2 patients.Total average operation time was (197±68) min, and the blood loss was (106±99) ml.The postoperative pancreatic fistula was observed in 7 patients, seroperitoneum was in 4 patients, incisional infection was in 1 patient, pulmonary infection was in 2 patients, and all the patients were cured by conservative treatment.There was no death during perioperative period.The postoperative hospital stay was (13±6)d.Analysis of pathological examination and clinical symptoms showed that 24 cases were diagnosed as functional pNENs (including 23 with insulinoma and 1 with gastrinoma), and 4 cases were as non-functional pNENs.The tumor grade G1 was categorized in 19 cases, G2 was in 9 cases.The TNM was I stage in all patients.There were no vascular or nerve invasion or lymph node metastasis.The follow-up period ranged from 4 to 144 months.2 patients were lost, and other patients were all alive.No diabetes or hypoglycemia occurred.Conclusions The function-preserving surgery for pNENs was safe and feasible, especially for well-differentiated functional pNENs.