The content of gastrointestinal stromal tumors had changed many times since the appearance of this idea. Along with the progress of molecular and biological study in recent years, it was a kind of tumor with specific clinical, pathological and molecular genetic characteristics, and was different from other specific tumors originated from the smooth muscle or nerves. Along with the success of targeted therapy of GIST, the disease was paid more attention. Therefore, a review of the clinical pathological study of GIST was needed.

Gastrointestinal stromal tumors is a new kind of tumor known recently, especially the important progress in the molecular and biologic study of GIST were used quickly in the clinical diagnosis and treatment, and made a close combination of basic research and clinical applications. The study and application of target therapy of new molecules on the mechanism of molecular genetics of GIST was another successful example. Therefore, GIST was paid extensive attention by the scholars. The progress on the molecular and biological study was summarized.

Objective To study the expression of E-cadherin protein and Snail protein in the labial glands of patients with primary Sj(o)gren's syndrome(pSS) and to analyze their roles in the pathogenesis of pSS.Methods The expression of E-cadherin protein and Snail protein were detected by immunohistochemical method.The labial glands were obtained from 50 patients with pSS and 30 healthy controls.The data was analyzed by Chi-Square test,Fisher's exact probability and Spearman's rank correlation analysis.Results The expression rate of E-cadherin in the labials gland of the patients with pSS were lower than that in normal salivary tissues (x2=14.651,P＜0.01).The positive rates of Snail in the labial glands of the patients with PSS were 40％ and it's expression rates were significantly higher than those in normal salivary tissues (x2=28.800,P＜0.01).There was a negative correlation between lymphocyte focus score and the expression of E-cadherin (P＜0.01) and a positive correlation between lymphocyte focus score and the expression of Snail in pSS group (P＜0.01).There was a negative correlation between the expression of E-cadherin and Snail (rs=-0.484,P＜0.01).Conclusion The interaction of E-cadherin and Snail may have played an important role in the pathogenesis of PSS.

Objective To study the protocol that rat bone marrow stromal cells (BMSCs) can be induced to differentiate into Schwann cell-like in vitro. Methods BMSCs were treated with beta-mercaptoethanol followed by retinoic acid and cultured in the presence of forskolin,basic-FGF,PDGF and heregulin. The positive percentages of GFAP,S-100 protein expression were measured by immunocytochemistry SABC staining. Results After the induction,BMSCs displayed morphologies of Schwann cell,such as ellipsoidal cell bodies and fences-like array,with two or three sligh cell processes. The positive percentages of GFAP protein expression was from 42.5% to 68.7%,S-100 was from 39.6% to 60.2%. Conclusions Rat BMSCs could be induced to Schwann cell-like by beta-mercaptoethanol,retinoic acid,forskolin,basic-FGF,PDGF and heregulin

Objective To evaluate the effectiveness of using induced bone marrow stromal cells into a tissue-engineered bioartificial nerve on bridging a 10mm long sciatic nerve defect Methods Twenty-eight F344 female rats weighing 160～200 g were randomly divided into four groups of nerve grafting,with 7 rats in each group A group:PLGA tubes with an intrinsic framework were seeded with syngeneic bone marrow stromal cells induced 5 days;B group:PLGA tubes with an intrinsic framework were seeded with syngeneic Schwann cells;C group:PLGA tubes were only filled with an intrinsic framework;D group:autografts Three months later,a series of examinations were performed,including:electrophysiological methods,Walking trace's analysis,hisotological staining of nerves,S-100 and neurofilament immunostaining and axon counts Results At 12 weeks after the operations,all the examinations of A group were better than C group( P 0.05). Conclusions Induced bone marrow stromal cells as seeding cells for peripheral nerve tissue engineering were used to repair 10mm nerve gap,the outcome was satisfied.

80 extracted human mandible premolars were divided into 10 groups(n =8)based on the post with different tilted angles(0°, buccal 15°and 30°,ligual 15°and 30°).The samples in experiment group were restored with fiber post while in the control group with cast post.Then all the teeth were restored with cast crown and bonded by glass ionomer.Compressive load with the speed of 1.0 mm/min was ap-plied to the restored teeth with a universal material testing machine until failure and the fracture modes were observed.The fracture load of fi-ber post and cast post restoration with the same tilted angles was similar(P >0.05).The main fracture mode of the teeth in all groups was unfavorable tooth fracture.No significant interaction was observed(P =0.217)between the 2 kinds of post and angulation of tilted teeth.

Objective To explore the role of integrin in CCL18 for promoting breast cancer SK-3 rd cells invasion and migration process to illuminate the molecular mechanism of CCL 18 for promoting breast cancer SK-3 rd cells invasion and migration process . Methods The flow cytometry was adopted to detect CCL18-induced integrin aggregation ;Western blot was used to detect the focal adhesion kinase(FAK ) activation ,the infiltrating migrationin experiment was adopted to determine the invasion and migration of SK-3rd cells and the siRNAs transfection was used to detect the expression of silence integrin β1 .Results CCL18 promoted the in-tegrinβ1 aggregation in breast cancer SK-3rd cell surface and further promoted the integrin-mediated phosphorylated activation of FAK .Under the reaction of CCL18 ,the cells number of SK-3rd cellular invasion and migration was increased by ten times (P<0 .01) ,which was obviously decreased by siRNA silenced integrin β1 .Conclusion CCL18 promotes breast cancer invasion and mi-gration via integrin aggregation .

OBJECTIVE: To study the mechanisms of the antitumor and immunoregulation functions of polyporus polysaccharide (PPS). METHODS: The production of nitric oxide (NO), the activity and mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages of mice administered with different dose of PPS were observed by Griess reaction, fluorimetry assay and RT-PCR, respectively. RESULTS: PPS could elevate the iNOS activity with dose-dependence and stimulate the iNOS mRNA expression of peritoneal macrophages in mice. CONCLUSION: The regulation of PPS on the production of NO in peritoneal macrophages of mice may occur at transcriptional level of iNOS. This indicates that the mechanism of PPS's antitumor and immunoregulation functions may be related to increasing NO output of macrophages through stimulating iNOS's denovo synthesis.

AIM:The effective abortifacient drug,mifepristone,was incorporated in the solid lipid nanoparticles (SLNs). The aim of this paper was to study the influence of ratios of drug to lipid on the characteristics of SLNs.METHOD:The physicochemical properties of the fine dispersed systems,such as the size distribution,Zeta potential had been analyzed by laser diffractometry(LD).The measurements of entrapment efficiency(EE)and thermal analysis of DSC were performed as well.RESULT:It was showed if the weight of lipid remained unchanged,the mean particle size of SLNs increased with the increase of drug amount.Drug entrapment efficiency was the highest while about 50 mg drug was loaded on weight 1 g lipid.Simultaneously,the charge of Zeta potential agreed well with the amount of free drug in the colloidal system.DSC analysis results showed that the melting peak of mifepristone at approx.190 ℃ disappeared.CONCLUSION:It was evidence that the drug incorporation would affect the average particle size and Zeta potential of the colloidal systems and the physical state of crystalline drug mifepristone in SLNs was present in the amorphous form or molecularly dispersed at even so high adding amount of 250 mg/g(lipid),which there was no report about the physical state of the drug and SLN with so high drug-loading.It was suggested that the model drug mifepristone could influence the property of nanodispersion system and the crystalline character of the drug was altered by the nanometer carrier system vice versa.

Logistics supplies are essential for normal operations of hospitals,while effective management of hospital suppliers is key to optimizing the materials supplies.An evaluation system is built for both key suppliers and standby suppliers respectively.Based on such a system,a dynamic management mechanism is built to provide long-lasting supplier incentives,for competitive selection and elimination of the suppliers.These efforts enable the hospitals to pick the best suppliers by means of systems and regulations.