Objective:To evaluate the diagnostic value of KaVo DIAGNOdent(DD)by comparing the diagnostic results of visual examination(VE)and stereomicroscope.Methods:The occlusal surface of 68 extracted teeth were examined by DD and VE,confirmed the examining points and marked,then cut these teeth along the markers.The results of stereomicroscope were classified into D0,D1,2,D3,4 according to the occlusal caries' depth,and recognized as gold standard.The difference of sensitivity,specificity between VE and DD,and their coherence were analyzed.Results:Sensitivity of DD,in D3,4 level of occlusal caries,was the highest.Compared to the results of stereomicroscope,the accuracy of DD and VE was the highest in D3,4 level.The coherence of DD and VE was low in all levels.Conclusion:The present cut-off point of DD affects the assessment of DD diagnostic result.Future study about key points of diagnosis of DD can widen its use in clinic.

The effect of Vitamin E on the metabolism of selenium in weaning Wistar rats raised with low selenium and low vitamin E Keshan disease diet and with the basal diet supplemented with selenium and/or a-tocopherol acetate was investigated. The results showed that:(1)On the same level of selenium in the diets, a-tocopherol supplemented to the diets might increase the selenium level of liver. No significant effect was found in other organs.(2)Whether the selenium was deficient in the diet or not, the GSHPx activities in blood, heart and liver of vitamin E deficient animals were lower than that of animals supplemented with vitamin E.(3)Supplementation of vitamin E to the diet decreased the selenium excretion in urine, and increased the selenium retention in tissues both in selenium deficient group and in selenium supplemented group.

Objective Delivery of oxygen in tissues is limited in the space where oxygen must diffuse between the vascular and the surrounding tissues. Normal capillaries are relatively straight and well spaced, in contrast, the tumor vascular networks usually display more irregularity and the vessel wall shows higher permeability and less elasticity. The purpose of this study was to investigate the effect of capillary elasticity and tortuosity on the oxygen distribution and make further investigation on the mechanism of the formation of hypoxic regions in tumor. Method One-dimensional capillary model was coupled with the oxygen diffusion model. Oxygen transport was investigated in a Krogh and tortuous tissue model. The capillarygeometrywas obtained bythe one-dimensional model and transferred to the tissue model. Finite element method was employed in the analysis. Result The capillary radii along the flow direction under pressures were obtained for different initial radii and the oxygen distribution in the Krogh cylinder tissue model and the model with a tortuous capillary were computed. Conclusions when the capillary radius is small, the effect of vessel elasticity may have not significant effect on the oxygen distribution. However, with the capillary radius increasing, the effect on the oxygen transport becomes obvious. Moreover, with the tortuosity of the capillary increasing, the oxygen distribution becomes more heterogeneous, which is in agreement with the result in available reference. This work will be helpful to the investigation of oxygen transport within tumor.

Objective To observe the changes of serum 25‐hydroxy vitamin D[25‐(OH)D] level of patients with rheumatoid ar‐thritis(RA) and study its clinical significance .Methods The method of chemiluminescence was adopted to detect peripheral blood serum 25‐(OH) D of 60 cases of RA patients(RA group) and 60 cases of healthy people(control group) .Results The serum 25‐(OH) D level of patients in RA group was(34 .37 ± 8 .87) nmol/L ,while that of the control group was(61 .33 ± 7 .74) nmol/L ;after comparison ,the differences were statistically significant (t= -6 .892 ,P< 0 .05);the serum 25‐(OH) D level of patients in RA group was in negative correlation with RA patient age ,body mass index(BMI) ,time of morning stiffness ,tender joint count(TJC) , swollen joint count(SJC) ,C‐reactive protein(CRP) ,erythrocyte sedimentation rate(ESR) ,visual analog scale(VAS) for disease o‐verall pain and health assessment questionnaire (HAQ) and disease activity score (DAS28)(r= -0 .201 ,-0 .255 ,-0 .362 ,-0 .296 ,-0 .273 ,-0 .198 ,-0 .323 ,-0 .356 ,-0 .379 ,-0 .418 ;P<0 .05 in all cases);but had no correlation with course of dis‐ease ,rheumatoid factor (RF) ,anti‐cyclic citrullinated peptide (CCP) antibody (anti‐CCP) and antibody concentration (P> 0 .05) . Conclusion The serum 25‐(OH) D level of RA patients is significantly low ,which might be associated with the RA disease activi‐ty .

In this paper, the importance of using advanced medical equipment to check treatment was ana-lyzed, and then pointed out that nowadays there were the following problems: the medical staff had excessive de-pendence on medical equipment; medical equipment inspection treatment of irrationality; medical equipment in-spection treatment with humanity was not enough; medical equipment management of dereliction of duty. Put for-ward to strengthen medical personnel professional moral education;to strengthen the medical students' ethical edu-cation;regular hospital manage concept;improve the cognitive level of medical staff of medical equipment.

Objective To investigate the effects of Ligustrazine on TNF-α-induced TGF-β and CTGF expression in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were isolated from human omenta by trypsin di-gestion. Then, the subcultured HPMCs were divided into control group, TNF-α-induced (1 μg/L) group and TNF-α-induced plus low-, medium-and high-dose Ligustrazine (10, 20 and 40 mg/L Ligustrazine respectively) groups. The viability of HPMCs was measured by MTT assay. RT-PCR was used to detect the expressions of TGF-β1 and CTGF mRNAs in HPMCs. TGF-β1 and CTGF in supernatants were measured by ELISA. Cell protein concentration was measured by trace bicinchoninic acid (BCA) method to validate the ELISA assay results. Results Ligustrazine significantly decreases TNF-α-induced TGF-β1 and CTGF expression in a dose-dependent manner at both protein and gene levels ( P < 0. 05 ). In addition, medium-and high-dose Ligustrazine injection significantly ameliorates the viability of HPMCs inhibited by TNF-α ( P < 0. 05 ). Conclusion Ligustrazine inhibits expressions of TGF-β and CTGF of HPMCs in an inflammatory conditions.

At present,there are many problems in the cultivation for medical students including lack of humanistic education,insufficient healthy personality shaping and innovation education.We made explorations in aspects of updating education concept,enhancing faculty construction,emphasizing basic course teaching and promoting students' abilities so as to cultivate the comprehensive quality of students majored in medical imaging (ultrasonic medicine).

Objective To investigate the effects of Ligustrazine on TNF-?-induced TGF-?1 and CTGF expression in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were isolated from human omenta by trypsin digestion. Then,the subcultured HPMCs were divided into control group,TNF-?-induced (1 ?g/L) group and TNF-?-induced plus low-,medium-and high-dose Ligustrazine (10,20 and 40 mg/L Ligustrazine respectively) groups. The viability of HPMCs was measured by MTT assay. RT-PCR was used to detect the expressions of TGF-?1 and CTGF mRNAs in HPMCs. TGF-?1 and CTGF in supernatants were measured by ELISA. Cell protein concentration was measured by trace bicinchoninic acid (BCA) method to validate the ELISA assay results.ResultsLigustrazine significantly decreases TNF-?-induced TGF-?1 and CTGF expression in a dose-dependent manner at both protein and gene levels (P

0.05) in the results of Vd of mannitol and glycerol for both rabbits and human studies

[Objective]The article summarizes the experience of Professor HE Lingzhi in treating purpura nephritis . [Methods]To collect and analyse the theory of HE's experience of herb pairs in treating purpura nephritis by studying from her for a long time and one medical record. [Results]Professor HE Lingzhi believes that the disease needs stage therapeutics and it is caused by pathogen wind pyretic pathogenic factor and poisonous evil in the stag of onset. Deficiency of both qi and yin in remission period. Syndrome of blood stasis consistently throughout purpura nephritis. Pro. HE treats purpura nephritis by pairing drugs,such as schizonepetae and honeysuckle, caulis sinomenii and saposhnikovia divaricata, astragalus membranaceus and eclipta alba, the root of red-rooted salvia and puccoon. [Conclusion] Professor HE's clinical experience in treating purpura nephritis from pairing drugs is well worth learning for it always gets outstanding effect.